The biochemical contents of plants may vary depending on soil and climatic conditions. Under unsuitable conditions, the resistance mechanismof plants is determined by the biochemical properties of the plants. For this reason, it is of great importance to determine thebiochemical contents of some plants that are naturally grown and generally used as dye plants. For this purpose, Salix albaL., Verbascum thapsusL., Urtica dioicaL., Digitalis lanataEhrh, Galium aparineL., Lythrum salicariaL., Hypericum perforatumL., Hypericum scabrumL., Quercus roburL. subsp. robur, Glycyrrhiza glabraL. plants were collected. Antioxidant enzyme activity, hormone, organic acid, total phenolic substance and some other biochemical analyzes were made in these plant samples. As a result of the study, depending on the plant varieties, total antioxidant, total phenolic content and organic acid levels showed significant differences. According to the results of this study, Verbascum thapsusL. plant had high antioxidant enzyme activity, while Digitalis lanataEhrh. plant was found to be higher in terms of hormone and total organic acid content. The highest amount of carotenoid, abscisic acid and hydrogen peroxide were determined in Urtica dioica L. plant. Different results were obtained in other plant varieties, and it was observed that a different biochemical content was more effective in each plant. For this reason, it has been determined that the biochemical contents of these plants, which have different uses. The usage area of this plant should be created after the main active ingredient is determined.