Inhibitory Effects on Melanin Production in B16 Melanoma Cells of Fallen Pear

Phlorotannins isolated from brown algae, such as Eisena bicyclis, have positive physiological effects, including anti-cancer, anti-inflammatory, and anti-Alzheimer’s disease. Although phlorotannins have been shown to inhibit tyrosinase, an enzyme essential for melanogenesis, their effect on melanogenesis remains unexplored. Thus, we isolated phlorotannins from E. bicyclis and examined their effects on α-melanocyte-stimulating hormone (α-MSH)-induced melanogenesis in murine B16 melanoma cells. Both fucofuroeckol-A (FF-A) and phlorofucofuroeckol-A (PFF-A) suppressed α-MSH-induced melanogenesis. Neither inhibited human tyrosinase (TYR) activity, but both inhibited tyrosinase-related protein-2 activity. FF-A downregulated the expression of microphthalmia-associated transcription factor and TYR, which subsequently suppressed melanin production. These results suggest that phlorotannins could be beneficial as melanin control drugs for hyperpigmentation disorders.

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The Effects of Salacia reticulata on Anti-Cellular Oxidants and Melanogenesis Inhibition in α-MSH-stimulated and UV Irradiated B16 Melanoma Cells

Natural Product Communications ◽

10.1177/1934578x1400900433 ◽

2014 ◽

Vol 9 (4) ◽

pp. 1934578X1400900

Author(s):

Prasit Sirwannalert ◽

Ryusho Kariya ◽

Ikuko Suzu ◽

Seiji Okada

Keyword(s):

Melanoma Cells ◽

Pharmaceutical Products ◽

B16 Melanoma ◽

Tyrosinase Activity ◽

Root Extract ◽

Dependent Manner ◽

Inhibitory Effects ◽

B16 Melanoma Cells ◽

Dose Dependent ◽

Salacia Reticulata

The purposes of this study were to investigate the inhibitory effects of Salacia reticulata Tul. root extract on cellular oxidants and melanogenesis in B16 melanoma cells. Cells treated with non-toxic doses of S. reticulata root extract were investigated for their effects on melanogenesis, cellular tyrosinase activity and cellular oxidant scavenging activity. The results indicated that S. reticulata extract inhibited melanin synthesis and tyrosinase activity in α-MSH-induced or UV-irradiated B16 melanoma cells in a dose dependent manner. Additionally, the extract also exhibited anti-cellular oxidants in UV-induced radical melanoma cells. Altogether, these results suggested that S. reticulata root extract has roles in suppression of melanogenesis and oxidant inhibition. S. reticulata root extract may be a potential source for the development of pharmaceutical products for treatment of skin hyperpigmentation disorders.

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ChemInform Abstract: Diarylheptanoids with Inhibitory Effects on Melanogenesis from the Rhizomes of Curcuma comosa in B16 Melanoma Cells.

ChemInform ◽

10.1002/chin.201403218 ◽

2014 ◽

Vol 45 (3) ◽

pp. no-no

Author(s):

Hisashi Matsuda ◽

et al. et al.

Keyword(s):

Melanoma Cells ◽

B16 Melanoma ◽

Inhibitory Effects ◽

Curcuma Comosa ◽

B16 Melanoma Cells

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Cinnamomum cassia Essential Oil Inhibits α-MSH-Induced Melanin Production and Oxidative Stress in Murine B16 Melanoma Cells

International Journal of Molecular Sciences ◽

10.3390/ijms140919186 ◽

2013 ◽

Vol 14 (9) ◽

pp. 19186-19201 ◽

Cited By ~ 45

Author(s):

Su-Tze Chou ◽

Wen-Lun Chang ◽

Chen-Tien Chang ◽

Shih-Lan Hsu ◽

Yu-Che Lin ◽

...

Keyword(s):

Oxidative Stress ◽

Essential Oil ◽

Melanoma Cells ◽

B16 Melanoma ◽

Melanin Production ◽

Cinnamomum Cassia ◽

B16 Melanoma Cells ◽

And Oxidative Stress

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The Inhibitory Effects of Representative Chalcones Contained in Angelica keiskei on Melanin Biosynthesis in B16 Melanoma Cells

Natural Product Communications ◽

10.1177/1934578x1200700812 ◽

2012 ◽

Vol 7 (8) ◽

pp. 1934578X1200700 ◽

Cited By ~ 1

Author(s):

Enos Tangke Arung ◽

Shoko Furuta ◽

Kazuhiro Sugamoto ◽

Kuniyoshi Shimizu ◽

Hiroya Ishikawa ◽

...

Keyword(s):

Melanoma Cells ◽

B16 Melanoma ◽

Inhibitory Effects ◽

Melanin Biosynthesis ◽

Melanin Formation ◽

B16 Melanoma Cells ◽

Angelica Keiskei ◽

Low Cytotoxicity ◽

Antioxidant Effects

In our effort to find new whitening agents, we evaluated the effects of representative chalcones [4-hydroxyderricin (1), xanthoangelol (2), xanthoangelol H (3), deoxyxanthoangelol H (4), and deoxydihydroxanthoangelol H (5)] contained in the stem of Angelica keiskei on tyrosinase and melanin formation in B16 melanoma cells. In addition, the antioxidant effects of these chalcones in ORAC and DPPH assays were also determined. Interestingly, all chalcones (1–5) inhibit melanin formation in B16 melanoma cells, with low cytotoxicity.

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