Isolation and Identification of Bacteria from Urinary Tract Infections

Background: Hands and cell phones are the major source of cross-transmission of urinary tract infections. The aim of this study was to isolate, identify and evaluate Gram-negative bacteria from hand and mobile phones. Methods: This study was conducted in visiting area of Civil Hospital Karachi, Pakistan. Analysis was done by 100 wet sterile cotton tipped swabs, 50 each from mobile phones and hands of their owners. Samples were transported in a Cary Blair transport media, Swabs were streaked on Nutrient agar, Blood agar and MacConkey agar. Organisms were identified by cultural, biochemical, and microscopic characteristics. Results: Total samples n=100 was collected from hand and mobile phones samples (50 hand and 50 mobile swabs) from the Dow university Hospital and Civil Hospital Karachi were tested. Six species of bacteria were isolated along with their identification during the research study. The isolated bacteria were Serratia, Klebsiella, Pseudomonas, Proteus, Shigella and Escherichia coli. The participants’ hands showed high bacterial contamination (50%-56%) in comparison to mobile phones. The frequency (%) of bacteria isolated from mobile phone and hand swabs included Serratia, 12 (24%) with the highest quantity and frequently found bacteria. While, the rest of the results reported Escherichia coli 10 (20%), Klebsiella 9 (18%), Pseudomonas 5 (10%), Shigella 4 (8%) and Proteus 10 (20%) respectively. Conclusion: Patient attendants in hospitals and visitors are more susceptible to nosocomial infections through exchange of mobile phones n=12(24%). Therefore, hygienic practice of hands cleaning while mobile using may help to break the transmission cycle of pathogenic bacteria.

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Phenotypic and genotypic detection of extended spectrum β-lactamases among Escherichia coli and Klebsiella pneumoniae isolates from type 2 diabetic patients with urinary tract infections

African Health Sciences ◽

10.4314/ahs.v21i2.3 ◽

2021 ◽

Vol 21 (2) ◽

pp. 497-504

Author(s):

Souad Youssouf Kani Elmi ◽

Medhat Saber Ashour ◽

Fathy Zakaria Alsewy ◽

Nashwa Fawzy Abd El Moez Azzam

Keyword(s):

Urinary Tract ◽

Urinary Tract Infections ◽

Diffusion Method ◽

Resistance Pattern ◽

Diabetic Patients ◽

Isolation And Identification ◽

E Coli ◽

Extended Spectrum ◽

Tract Infections

Background: T2DM patients are more likely to have UTIs caused by resistant organisms such as ESBLs producing bacteria. Challenging reliable identification and prompt characterization of in-vitro susceptibilities of these bacteria are the first steps of deciding the appropriate antimicrobial therapy for UTIs caused by them. Objectives: To isolate and identify E. coli and K. pneumoniae from urine of T2DM patients with UTIs, to determine antibiotic resistance pattern among isolates, and to identify ESBLs production phenotypically and genotypically. Material and method: All samples were cultured on Cystine-Lactose-Electrolyte-Deficient Agar medium (CLED) by using calibrated loop. Growth of 100 colonies or more, i.e. 105 colony forming units (CFU)/mL urine was considered as signifi- cant bacteriuria. Isolation and identification were done according to standard method. All isolates were tested for antibiotic susceptibility testing by the disc diffusion method according to CLSI guidelines. Phenotypic detection of ESBLs was done by double-disk synergy test. Genotypic detection of blaTEM, blaSHV and blaCTX-M genes by using PCR. Results: Results of this study showed that E. coli and K. pneumoniae were the dominant bacterial isolates, they constituted 103 (91.2%) out of 113 urine isolates. E. coli (58. 4%) K. pneumoniae (32.7%), Enterococcus spp. (4.4%), Proteus spp. (2.7%) and Pseu- domonas spp. (1.8%). About 25 (24.3%) out of 103 E. coli and K. pneumoniae isolates were ESBLs positive by DDST, and 22 (88.0%) out of them had ESBLs encoding genes by conventional PCR. The most common gene detected was blaTEM (59.1%), followed by blaSHV (27.3%). CTX-M had not been detected in any of testes isolates. Conclusion: blaTEM and blaSHV genes were detected in 22 out of 25 ESBLs producing E. coli and K. pneumoniae isolates phenotypically detected by DDST. blaTEM was found to be the predominant gene (59.1%), while blaCTX-Mene was not detected in any of tested isolates. Keywords: Extended Spectrum β-Lactamases; Type 2 diabetes mellitus; Urinary tract infections; Phenotypic; genotypic methods.

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Micro-test system for rapid isolation and identification of Candida species in urinary tract infections

Anthropological Researches and Studies ◽

10.26758/7.1.7 ◽

2017 ◽

Vol 7 (1) ◽

pp. 64-70

Author(s):

Nicolae Puscas ◽

◽

Greta Balan ◽

Olga Burduniuc ◽

◽

...

Keyword(s):

Urinary Tract ◽

Urinary Tract Infections ◽

Candida Species ◽

Test System ◽

Isolation And Identification ◽

Rapid Isolation ◽

Tract Infections

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Prevalence and Antibiotic Resistance of Bacteria Isolated from Urinary Tract Infections of Pregnant Women in Baghdad Hospitals

Biomedical & Pharmacology Journal ◽

10.13005/bpj/1573 ◽

2018 ◽

Vol 11 (4) ◽

pp. 1989-1994

Author(s):

Kais Kassim Ghaima ◽

Zainab Shaban Khalaf ◽

Alaa Aziz Abdulhassan ◽

Noor Yahya Salman

Keyword(s):

Antibiotic Resistance ◽

Urinary Tract ◽

Pregnant Women ◽

Urinary Tract Infections ◽

Age Groups ◽

Resistance Pattern ◽

Isolation And Identification ◽

Microbiological Methods ◽

Resistance Patterns ◽

Tract Infections

The aim of this study was investigating the drug resistance patterns of bacteria isolated from pregnant women with urinary tract infections (UTIs). This study included 1140 pregnant women attending two of main hospitals in Baghdad, Iraq, between September 2016 and August 2017. The isolation and identification of uropathogenic bacteria conducted by standard microbiological methods and Vitek2 system. The disc diffusion test was used to determine the antimicrobial resistance patterns of the isolated bacteria. The present study revealed that three hundred and fifty six isolates were positive for significant bacterial growth. Escherichia coli were the predominant bacteria (34 %) followed by Staphylococcus aureus (22.2 %), Klebsiella spp. (14.6 %), non-coagulase Staphylococcus (11.5 %), Proteus spp. (4.5 %), Pseudomonas spp. (3.7 %), Acinetobacter spp. (2.8 %), Citrobacter (2.8 %), Enterococcus (2.5 %) and Enterobacter (1.4 %). High resistance to Ampicillin (85.6 %), Co-trimoxazole (72.2 %) and Tetracycline (71.3 %) was observed. Also, It was found a moderate resistance to Ceftazidime, Ciprofloxacin, Amoxicillin-clavulanic acid and Ceftriaxone. Imipenem was the most active antibiotic against isolated uropathogens. Among the 356 uropathogenic isolates, 196 (55 %) were from women in the 21 to 30 years old age group, and this rate was higher than other age groups. In conclusion, urinary tract infections are frequent among pregnant women. Early detection and continuous monitoring of antibiotic resistance pattern is very important to determine the appropriate treatment of UTIs among pregnant women without complications.

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