scholarly journals The Effect of Long Storage of Whole Blood Components on the Level of 2,3 Diphosphoglycerate and Lactic Acid in the Blood Bank, Dr. Soetomo General Hospital, Surabaya, Indonesia

Author(s):  
Ashu Dogra ◽  
Devanshi Gosai

Background: Blood is precious and there is no alternative for human blood. Proper utilization as well as rationale use of blood is necessary with minimal discarding of blood units and implementing various interventions that can be used to optimize blood and its components use by training and education. Aim: An analytical study of discarded units of whole blood and its components in a blood bank at a Tertiary care hospital in vadodara. Study designs and methods: Data on the number of discarded whole blood units and its components, reasons for discard, number of blood components processed as well as the number of collected blood units were obtained from blood bank records and registers. The data obtained was analyzed. Results: The total number of blood units collected from Jan 2016 to Dec 2018 was 13249 from which 36447 units of components were prepared. The total number of discarded whole blood units and its components was 5097.


Transfusion ◽  
2021 ◽  
Vol 61 (S1) ◽  
Author(s):  
Julia H. Kolodziej ◽  
Julie C. Leonard ◽  
Cassandra D. Josephson ◽  
Barbara A. Gaines ◽  
Stephen R. Wisniewski ◽  
...  

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Ruby E. Harrison ◽  
Mark R. Brown ◽  
Michael R. Strand

Abstract Background Most female mosquitoes are anautogenous and must blood feed on a vertebrate host to produce eggs. Prior studies show that the number of eggs females lay per clutch correlates with the volume of blood ingested and that protein is the most important macronutrient for egg formation. In contrast, how whole blood, blood fractions and specific blood proteins from different vertebrates affect egg formation is less clear. Since egg formation is best understood in Aedes aegypti, we examined how blood and blood components from different vertebrates affect this species and two others: the malaria vector Anopheles gambiae and arbovirus vector Culex quinquefasciatus. Methods Adult female mosquitoes were fed blood, blood fractions and purified major blood proteins from different vertebrate hosts. Markers of reproductive response including ovary ecdysteroidogenesis, yolk deposition into oocytes and number of mature eggs produced were measured. Results Ae. aegypti, An. gambiae and C. quinquefasciatus responded differently to meals of whole blood, plasma or blood cells from human, rat, chicken and turkey hosts. We observed more similarities between the anthropophiles Ae. aegypti and An. gambiae than the ornithophile C. quinquefasciatus. Focusing on Ae. aegypti, the major plasma-derived proteins (serum albumin, fibrinogen and globulins) differentially stimulated egg formation as a function of vertebrate host source. The major blood cell protein, hemoglobin, stimulated yolk deposition when from pigs but not humans, cows or sheep. Serum albumins from different vertebrates also variably affected egg formation. Bovine serum albumin (BSA) stimulated ovary ecdysteroidogenesis, but more weakly induced digestive enzyme activities than whole blood. In contrast, BSA-derived peptides and free amino acids had no stimulatory effects on ecdysteroidogenesis or yolk deposition into oocytes. Conclusions Whole blood, blood fractions and specific blood proteins supported egg formation in three species of anautogenous mosquitoes but specific responses varied with the vertebrate source of the blood components tested.


2005 ◽  
Author(s):  
Hans C. Fischer ◽  
Eliseo Papa ◽  
Lichuan Liu ◽  
K. Sandy Pang ◽  
Warren C. W. Chan

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 999-999
Author(s):  
Fabiola Grizzatti ◽  
Melissa A Qazi ◽  
David Stroncek ◽  
Nathawut Sibmooh ◽  
Barbora Piknova ◽  
...  

Abstract In light of recent papers stressing the importance of decreased levels of SNO-hemoglobin (SNO-Hb) to the pronounced nature of deleterious effects of transfusion of stored red blood cells (RBCs), there has been an increased interest in the practice of blood storage. Dejam et al. (Blood, 2005) previously demonstrated the critical nature of RBCs in NO physiology: they serve as the major intravascular stores of nitrite, which is eventually converted to NO, an important player in vasoregulation. The purpose of this study is to quantify the NO metabolites, nitrite and nitrate, in three blood components and evaluate their levels over time of storage. Blood obtained from 6 healthy volunteer donors was split into whole blood, leukoreduced, and non-leukoreduced packed RBCs and stored in polyvinyl chloride (PVC) bags for 42 days at 4°C. PVC bags were maintained in either room air or an argon chamber. Nitrite, nitrate, and SNO-Hb/nitrosyl-hemoglobin (HbNO) were measured using reductive gas-phase chemiluminescence. In all blood components, the nitrite and nitrate were detected in higher concentrations in RBCs than in the extracellular fluid compartment. Mean nitrite value immediately before storage was 152±13nM, but fell rapidly upon storage. Nitrite levels continued to decrease with storage time, while nitrate levels remained constant for the duration of storage. In the leukoreduced blood product, nitrite levels were 75±8nM on day 1 and 50±9nM by day 42; the concentration of nitrate in the leukoreduced blood product was 34±3uM on day 1 and 34±4uM on day 42. The nitrite levels in non-leukoreduced blood product were 76±12nM on day 1 and 37±7 by day 42; the nitrate concentration in the non-leukoreduced blood product was 35±3uM on day 1 and 32±0.4uM on day 42. In whole blood, nitrite levels were 64±11nM on day 1 and 44±9nM by day 42; the nitrate concentration was 47±2uM on day 1 and 43±6uM on day 42. SNO-Hb levels were very low in fresh blood and virtually undetectable after one day of storage. Interestingly, nitrite levels never reached zero. Enzyme inhibitors—L-NAME (nitric oxide synthase inhibitor), acetazolamide (carbonic anhydrase inhibitor), and oxypurinol (xanthine oxidase inhibitor)—did not lower nitrite levels enough to explain the remaining nitrite present in the PVC bags after 42 days. pH decreased slightly, while pO2 increased in all three components during storage; this is likely due to the diffusion of oxygen from room air into the PVC bags. Control experiments with saline showed an increase in nitrite levels, while nitrate levels remained stable over 42 days. When stored in an argon chamber, both blood and saline samples showed relatively lower nitrite levels than their room air counterparts. Thus, during blood bank storage, nitrite levels decrease in blood while nitrate levels remain stable. The diffusion of nitrogenous gases may explain why nitrite does not completely disappear under standard storage conditions. Our results suggest that most of the NO pathway is initially retained, but greater changes occur with prolonged storage. These measurements of NO derivatives may have implications for transfusion therapy, explaining some of the adverse effects seen with RBC transfusion and providing a foundation for enhancing blood preservation through improvement of storage practices.


2021 ◽  
Vol 8 ◽  
Author(s):  
Thomas H. Edwards ◽  
Anthony E. Pusateri ◽  
Erin Long Mays ◽  
James A. Bynum ◽  
Andrew P. Cap

Since the inception of recent conflicts in Afghanistan and Iraq, transfusion practices in human military medicine have advanced considerably. Today, US military physicians recognize the need to replace the functionality of lost blood in traumatic hemorrhagic shock and whole blood is now the trauma resuscitation product of choice on the battlefield. Building on wartime experiences, military medicine is now one of the country's strongest advocates for the principle of hemostatic resuscitation using whole blood or balanced blood components as the primary means of resuscitation as early as possibly following severe trauma. Based on strong evidence to support this practice in human combat casualties and in civilian trauma care, military veterinarians strive to practice similar hemostatic resuscitation for injured Military Working Dogs. To this end, canine whole blood has become increasingly available in forward environments, and non-traditional storage options for canine blood and blood components are being explored for use in canine trauma. Blood products with improved shelf-life and ease of use are not only useful for military applications, but may also enable civilian general and specialty practices to more easily incorporate hemostatic resuscitation approaches to canine trauma care.


2002 ◽  
Vol 126 (8) ◽  
pp. 909-914 ◽  
Author(s):  
David A. Novis ◽  
Richard C. Friedberg ◽  
Stephen W. Renner ◽  
Frederick A. Meier ◽  
Molly K. Walsh

Abstract Objectives.—To determine the normative distribution of time elapsed for blood bank personnel to fill nonscheduled operating room (OR) blood component orders in hospital communities throughout the United States, and to examine hospital blood bank practices associated with faster blood component delivery times. Design.—Participants in the College of American Pathologists Q-Probes laboratory quality improvement program collected data prospectively on the times elapsed for blood bank personnel to fill nonscheduled emergent orders from hospital ORs for red blood cell (RBC) products, fresh frozen plasma (FFP), and platelets (PLTs). Participants also completed questionnaires describing their hospitals' and blood banks' laboratory and transfusion practices. Setting and Participants.—Four hundred sixty-six public and private institutions located in 48 states in the United States (n = 444), Canada (n = 9), Australia (n = 8), the United Kingdom (n = 4), and Spain (n = 1). Main Outcome Measures.—The median time elapsed between requests for blood components by OR personnel and the retrieval of those components by blood component transport personnel, and the median time elapsed between requests for blood components by OR personnel and the arrival of those components in ORs. Results.—Participants submitted data on 12 647 units of RBCs, FFP, and PLTs. The median aggregate request-to-retrieval turnaround times (TATs) for RBCs, FFP, and PLTs ranged from 30 to 35 minutes, and the median aggregate request-to-arrival TATs for RBCs, FFP, and PLTs ranged from 33 to 39 minutes. Most of the TAT was consumed by events occurring prior to, rather than after release of components from blood banks. Shorter prerelease TATs were associated with having surgical schedules that listed patients' names and procedures available to blood bank personnel prior to surgeries, and having adequate clotted specimens in the blood bank and completed type-and-screen procedures performed before requests for blood components were submitted to blood banks. Among the fastest-performing 10% of participants (90th percentile and above), request-to-retrieval TATs ranged from 12 to 24 minutes for the 3 blood components, whereas among the slowest-performing 10% of participants (10th percentile and below), request-to-retrieval TATs ranged from 63 to 115 minutes for the 3 components. Median TATs ranged from 33 to 37 minutes for the 3 components. Institutions with TATs in the fastest-performing 25th percentile more frequently stored cross-matched RBCs in the OR daily, stocked PLTs for unexpected surgical use, stored PLTs in or near the OR, and had laboratory rather than nonlaboratory personnel deliver components to the OR than did those institutions with TATs in the slowest-performing 25th percentile. Conclusions.—Hospital blood bank personnel can deliver blood components to the OR in slightly longer than 30 minutes, measured from the time that those units are requested by OR personnel. Practices aimed at saving time before components are released from blood banks will be more efficient in reducing overall TAT than those practices aimed at saving time after components are released from blood banks. Specific practices associated with shorter blood delivery TATs included providing blood bank personnel with access to the names of surgical patients potentially requiring blood components, having pretransfusion testing completed on those patients prior to surgery, having ample blood products on hand, and having laboratory personnel control blood product delivery.


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