scholarly journals DESARROLLO EMBRIONARIO DEL MERO PIEDRERO Epinephelus labriformis (jENYNS, 1840) y DESCRIPCIÓN DEL ELEUTEROEMBRIÓN

2015 ◽  
Vol 30 (2) ◽  
pp. 27
Author(s):  
Pablo Torres -Hernández ◽  
Paulina Cebada -Martínez ◽  
Diego A. Girón -Cruz ◽  
Germán I. Garrido -Fariña ◽  
Carlos A. Alvarez -González
Keyword(s):  
Embryonic Development ◽  
Average Length ◽  
Average Diameter ◽  
Yolk Sac ◽  
Biological Knowledge ◽  
Central Position ◽  
Vitro Fertilization ◽  
Hatching Time ◽  
Controlled Reproduction

A partir del desove inducido y la fertilización in vitro del huevo del mero piedrero Epinephilus labriformis se describe el desarrollo embrionario y el eleuteroembrión. La inducción a la maduración y desove se realizó con dos inyecciones de 700 UI GCH∙kg-1, se procedió a realizar el desove y fertilización in vitro. El huevo se colocó en incubadoras de 6 L, a 35 UPS y se cultivó a 23, 24, 25 y 27°C hasta la eclosión. Se realizó la descripción de los estadios de segmentación, gastrulación, nerula y hasta la eclosión del eleuteroembrión a 25ºC. Se reporta el promedio ± desviación estándar del diámetro de huevo, el diámetro de la gota de aceite, la longitud notocordial y la apertura de la boca. El huevo de E. labriformis es pelágico, con corión liso y con una sola gota de aceite en posición central. El diámetro del huevo fue 726.1 ± 18.4 μm y de la gota de aceite 135.5 ± 17 μm. En el cultivo a 25º C, la primera segmentación se observó a los 40 min, siendo la discoblástula evidente a tres horas despúes la fertilización. La nerula con vesículas ópticas, somitas bien definidos y con presencia de melanóforos a las 25 horas, eclosionando a las 27 horas posteriores a la fertilización. La longitud notocordal fue 1,327 ± 186.3 μm, diámetro de la gota de aceite de 131.3 ± 17.1 μm, longitud del saco vitelino de 526.2 ± 72.8 μm y su altura 359.6 ± 74.6. La ecuación que describe el tiempo de eclosión (Te) con respecto a la temperatura de incubación (Tinc) fue: Te=119.68–(3.5793xTinc), en el intervalo de 23 a 27°C. La descripción del desarrollo embrionario y del eleuteroembrión contribuye en el conocimiento biológico de la especie y en el desarrollo de metodologías para su reproducción controlada y cultivo larval. Embryonic development of the starry grouper Epinephelus labriformis (Jenyns, 1840) and description of its eleutheroembryo From the induced spawning and in vitro fertilization of the eggs of the starry grouper Epinephelus labriformis the embryonic development and the eleutheroembryo were described. The spawning induction was performed with two injections of 700 UI GCH∙kg-1 to carry out spawning and artificial fertilization. The viable eggs were placed in incubators of 6 L with 35 UPS and raised at 23, 24, 25, and 27 °C. A chronological description of segmentation stages, gastrulation, and nerula was made until hatching of the eleutheroembryo at 25°C. The average + standard deviation of the egg diameter and the diameter of oil drop from the eleutheroembryo were reported along with the notocordial length, yolk sac length, and diameter of the eleutheroembryo´s oil drop. The egg is pelagic, spherical, transparent, smooth chorion with only one oil drop in a central position. The average diameter of the eggs is 726.1 + 18.4 μm and 135.5 + 17 μm for the oil drop. At 25°C the first segmentation was observed after 40 minutes post fertilization and the discoblastula is evident at 3 hours post fertilization. The nerula with optical vesicles, well-defined somites, and the presence of melanophores was observed at 25 hours. Eggs hatched at 27 hours post fertilization. The notochord average length of the eleuteroembryo was 1,327 + 186.3 μm, the diameter of the oil drop is 131.3 + 17.1 μm. The length of the yolk sac is 526.2 + 72.8 μm and its height is 359.6 + 74.6 μm. The equation that describes hatching time (Te) with respect to the temperature (Tinc) was: Te = 119.68 – (3.5793 x Tinc) in an interval of 23°C to 27°C (n=8; r2=0.8631; p=0.0008). The description of the embryonic development and eleutheroembryo contribute to the biological knowledge of the species and the development of methods the controlled reproduction and larviculture of E. labriformis.

scholarly journals Decreased miR-149 expression in sperm is correlated with the quality of early embryonic development in conventional in vitro fertilization

2021 ◽  
Vol 101 ◽  
pp. 28-32
Author(s):  
Hongping Li ◽  
Lejun Li ◽  
Chuanping Lin ◽  
Minhao Hu ◽  
Xiaozhen Liu ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryonic Development ◽  
Early Embryonic Development ◽  
Vitro Fertilization

scholarly journals MicroRNA Expression Profiles Analysis in Sperm Reveals hsa-mir-191 is an Auspicious Omen of in Vitro Fertilization

2019 ◽  
Author(s):  
Hua Xu ◽  
Xin Wang ◽  
Zhikai Wang ◽  
Jianhui Li ◽  
Zhiming Xu ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryonic Development ◽  
Roc Curve ◽  
Small Rnas ◽  
Expression Profiles ◽  
High Quality ◽  
Vitro Fertilization ◽  
The Relationship ◽  
Early Human

Abstract Background: MicroRNAs (miRNAs) are a class of noncoding small RNAs that play important roles in many physiological processes by regulating gene expression. Previous studies have shown that the expression levels of total miRNAs increase during mouse embryonic development, and some miRNAs control the regulatory network in development progression. However, few studies have focused on the effects of miRNAs on early human embryonic development. The relationship between miRNAs and early human embryogenesis is still unknown. Results:In this study, RNA-seq data collected from sperm samples from 102 patients with a normal sperm index but treated with assisted reproductive technology (ART) were analyzed for the relationships between differentially expressed small RNAs and the fertilization rate (FR), blastocyst rate and high-quality embryo rate (HQER). The sperm samples with high hsa-mir-191 expression had a higher FR, effective embryo rate (EER) and HQER. hsa-mir-191 was used as a single indicator to predict the HQER. The receiver operating characteristic (ROC) curve had an area under the ROC curve (AUC) of 0.686. We also found that hsa-mir-191 expression is correlated with an abnormal sperm rate (cor = 0.29, p< 0.01). We also evaluated the relationship between hsa-mir-34c and early human embryo development in these 102 sperm samples and obtained negative results. Conclusions: These findings suggest that high hsa-mir-191-5p expression in sperm is associated with early human embryonic quality and that hsa-mir-191-5p could be used as a potential marker to screen high-quality sperm to improve the success rates of in vitro fertilization (IVF).

scholarly journals MicroRNA Expression Profiles Analysis in Sperm Reveals hsa-mir-191 is an Auspicious Omen of in Vitro Fertilization

2019 ◽  
Author(s):  
Hua Xu ◽  
Xin Wang ◽  
Zhikai Wang ◽  
Jianhui Li ◽  
Zhiming Xu ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryonic Development ◽  
Roc Curve ◽  
Small Rnas ◽  
Small Rna Sequencing ◽  
High Quality ◽  
Vitro Fertilization ◽  
The Relationship ◽  
Early Human

Abstract Background : MicroRNAs (miRNAs) are a class of noncoding small RNAs that play important roles in many physiological processes by regulating gene expression. Previous studies have shown that the expression levels of total miRNAs increase during mouse embryonic development, and some miRNAs control the regulatory network in development progression. However, few studies have focused on the effects of miRNAs on early human embryonic development. The relationship between miRNAs and early human embryogenesis is still unknown. Results: In this study, sperm samples from 102 patients with a normal sperm index but treated with assisted reproductive technology (ART) were collected for small RNA sequencing, and the relationships between differentially expressed small RNAs and the fertilization rate (FR), blastocyst rate and high-quality embryo rate (HQER) were analyzed. The sperm samples with high hsa-mir-191 expression had a higher FR, effective embryo rate (EER) and HQER. hsa-mir-191 was used as a single indicator to predict the HQER. The receiver operating characteristic (ROC) curve had an area under the ROC curve (AUC) of 0.686. We also found that hsa-mir-191 expression is correlated with an abnormal sperm rate (cor = 0.29, p < 0.01). We also evaluated the relationship between hsa-mir-34c and early human embryo development in these 102 sperm samples and obtained negative results. Conclusions: These findings suggest that high hsa-mir-191-5p expression is associated with improved early human embryonic development and that hsa-mir-191-5p could be used as a potential marker to screen high-quality sperm to improve the success rates of in vitro fertilization (IVF).

Fertilization and early embryology: The effect of pentoxifylline on mouse in-vitro fertilization and early embryonic development

1994 ◽  
Vol 9 (10) ◽  
pp. 1903-1908 ◽  
Author(s):  
Herman Tournaye ◽  
Marleen Van der Linden ◽  
Etienne Van den Abbeel ◽  
Paul Devroey ◽  
André Van Steirteghem
Keyword(s):  
In Vitro Fertilization ◽  
Embryonic Development ◽  
Early Embryonic Development ◽  
Vitro Fertilization ◽  
Mouse In Vitro Fertilization

scholarly journals SUN-715 IIM May Influence Matured Oocytes’ DNA Methylation of PCOS Patients

2020 ◽  
Vol 4 (Supplement_1) ◽  
Author(s):  
Congru Li ◽  
Yang Yu
Keyword(s):  
Dna Methylation ◽  
In Vitro Fertilization ◽  
Embryonic Development ◽  
Embryo Transfer ◽  
Cpg Island ◽  
Reproductive Technologies ◽  
Childbearing Age ◽  
Vitro Fertilization ◽  
The Impact

Abstract Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in women of childbearing age and is the main cause of anovulatory infertility. To increase the number of oocytes obtained, controlled ovarian stimulation (COS) has become a routine choice for in vitro fertilization-embryo transfer (IVF-ET), which is one of the common assisted reproductive technologies for PCOS patients. However, for these patients, there is a high risk of ovarian hyperstimulation syndrome (OHSS). Obtaining in vitro maturation (IVM) of immature oocytes, and then in vitro fertilization and embryo transfer of mature oocytes provides a possible way for people to solve the above problems. Since the IVM technology will expose oocytes to in vitro conditions for a longer period of time, theoretically increasing the risk of the oocytes being affected by the culture environment, further research and explorations are needed for study in gene programming, epigenetics, etc. Therefore, to explore the impact of IVM operation on embryonic development is of great significance for further clarifying assisted reproductive safety and improving IVM operation conditions. Here we focused on DNA methylation reprogramming process which was essential for embryonic development. We tested the DNA methylation of sperm, IVM oocytes and IVM generated early stage embryos including pronucleus, 4cell, 8cell, morula, inner cell mass, trophoectoderm (TE) as well as six-week embryos by Nimble Gen Human DNA Methylation 3x729K CpG Island Plus RefSeq Promoter Array and compared the data with our published genome-wide DNA methylomes of human gametes and early embryos generated from in vivo maturation oocytes. We showed that IVM embryos show abnormal DNA methylation reprogramming pattern. By analyzing the abnormally reprogrammed promoters, we further found that IVM may affect the functions of demethylation related genes. Oocytes from IVM manipulation were tested with higher DNA methylation levels, and their abnormal methylated promoters mainly enriched in immune and metabolism pathways. Furthermore, we investigated the DNA methylation of TE, which was directly related with implantation process and revealed the abnormal methylated promoters were related with metabolism pathway too. Our data support that IVM may influence the DNA methylome of oocytes, which in turn affects the methylome of their embryos. However, due to the limited number of samples and the inability of the chip to cover all CpG sites, the results of this study require further research and validation.

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