scholarly journals A Molecular Phylogenetic Study of Clarohilum henningsii (Mycosphaerellaceae, Fungi) on Cassava from Indonesia based on the ITS rDNA Sequence

2020 ◽  
Vol 2 (1) ◽  
pp. 40-45
Author(s):  
Indriati Ramadhani, M.Si. ◽  
Iman Hidayat, Ph.D.
Keyword(s):  
Leaf Spot ◽  
Internal Transcribed Spacer ◽  
Rdna Sequence ◽  
Its Rdna ◽  
Phylogenetic Position ◽  
Phylogenetic Study ◽  
Common Disease ◽  
Morphological Examination ◽  
Molecular Phylogenetic Study ◽  
Molecular Phylogenetic

Leaf spot is a common disease of cassava in Indonesia and other tropical countries. The present study aims to determine two isolates isolated from leaf spot of cassava in Indonesia using a molecular phylogenetic analysis based on internal transcribed spacer (ITS) rDNA sequence and morphological examination. The results showed that the two isolates belong to Clarohilum henningsii. This study revealed the phylogenetic position of C. henningsii from Indonesia, where in the previous studies, it has been reported as Cercospora cassavae, C. manihotis, C. henningsii, or Passalora henningsii based on morphological examinations.

scholarly journals Phylogenetic Study of Curvularia on Sorghum from Indonesia Based on ITS rDNA Sequence

2019 ◽  
Vol 3 (2) ◽  
pp. 118
Author(s):  
Iman Hidayat ◽  
Indriati Ramadhani
Keyword(s):  
Elongation Factor ◽  
Species Level ◽  
Rdna Sequence ◽  
Its Rdna ◽  
Phylogenetic Study ◽  
Translation Elongation Factor ◽  
Morphological Examination ◽  
Translation Elongation ◽  
Molecular Phylogenetic ◽  
Elongation Factor 1

Seven species of Curvularia were recorded from Indonesia, including species that pathogen to sorghum (Sorghum bicolor). The present study aims to determine four isolates of Curvularia isolated from sorghum in Indonesia using a combination of molecular phylogenetic analysis based on internal transcribed spacer (ITS) rDNA sequence and morphological examination. The results showed that the four isolates were genetically closed to C. lunata and C. dactyloctenicola. The morphological examination also insufficient to determine the identity of the four Curvularia isolates from sorghum until species level. Therefore, additional sequences from the partial fragments of the glyceraldehyde-3-phosphate dehydrogenase and the translation elongation factor 1-a genes are necessary to determine the identity of the Curvularia sequences isolated from sorghum in Indonesia until species level.

Phylogenetic position and taxonomic assignment of Thlaspi aghricum P.H.Davis & K.Tan (Brassicaceae)

Phytotaxa ◽  
2014 ◽  
Vol 178 (4) ◽  
pp. 287
Author(s):  
Mehmet Fırat ◽  
Barış Özüdoğru ◽  
Burcu Tarıkahya Hacıoğlu ◽  
Ali Savaş Bülbül ◽  
Ihsan Ali Al-Shehbaz ◽  
...  
Keyword(s):  
Taxonomic Status ◽  
Molecular Data ◽  
New Combination ◽  
Phylogenetic Position ◽  
Phylogenetic Study ◽  
Taxonomic Assignment ◽  
Molecular Phylogenetic Study ◽  
Molecular Phylogenetic ◽  
Morphological And Molecular Data ◽  
Seed Coat Morphology

A general review of the taxonomic status of Thlaspi past and present is given, and a critical evaluations of its segregates based on both morphological and molecular data are  presented. ITS molecular phylogenetic study of Thlaspi aghricum and related species, as well as seed-coat morphology and anatomy strongly support the placement of the species in Noccaea. The new combination N. aghrica is proposed, and detailed description and distribution of the species are given.

scholarly journals Carajasia (Rubiaceae), a new and endangered genus from Carajás mountain range, Pará, Brazil

Phytotaxa ◽  
2015 ◽  
Vol 206 (1) ◽  
pp. 14 ◽  
Author(s):  
Roberto Manuel Salas ◽  
PEDRO L. VIANA ◽  
ELSA L. CABRAL ◽  
STEVEN DESSEIN ◽  
STEVEN JANSSENS
Keyword(s):  
New Genus ◽  
Morphological Characters ◽  
Phylogenetic Position ◽  
Phylogenetic Study ◽  
Mountain Range ◽  
Distribution Maps ◽  
Dichotomous Key ◽  
Molecular Phylogenetic Study ◽  
Pollen Types ◽  
Molecular Phylogenetic

Carajasia is described as a new genus of Rubiaceae. It is so far known only from the mountain summits of Serra dos Carajás (Pará, Brazil), where it is part of a shrubby vegetation surrounded by tropical rainforest. The new genus belongs to the tribe Spermacoceae and is positioned within it to the Spermacoce clade. Carajasia is unique within the clade in having a very particular combination of characters: flowering branches with two axillary flowers per node, homostylous flowers, corollas with a fringe of moniliform hairs, pubescent styles with distinct stigma lobes, bilobed nectariferous discs covered by triangular papillae, pollen with a double reticulum and fruits with a peculiar type of dehiscence. A detailed description of Carajasia is presented, including observations of the fruit and pollen, along with distribution maps and images of the plant in its habitat. A dichotomous key to distinguish Carajasia from other genera with deeply divided stigmas is provided. A molecular phylogenetic study was carried out using ITS and ETS sequences to determine the phylogenetic position of the new genus within the Spermacoce clade. The results of the combined analyses demonstrated that Carajasia is sister to Galianthe with moderate to high support. Both genera form a weakly supported clade with Schwendenera. This clade is sister to the other genera of the Spermacoce clade studied in this work. Galianthe and Schwendenera share with Carajasia pollen with a double reticulum, but they are clearly differentiated by suffruticose habit, heterostylous flowers and the pattern of fruit dehiscence. To clarify the phylogenetic position of Carajasia, some morphological characters are discussed based on the molecular results: division of the stigma, pollen types and floral syndrome.

scholarly journals Phylogenetic position of Miliusa vidalii (Miliuseae, Annonaceae) inferred from chloroplast DNA and morphology

2020 ◽  
Vol 13 (2) ◽  
Keyword(s):  
Bayesian Inference ◽  
Chloroplast Dna ◽  
Maximum Parsimony ◽  
Sequence Data ◽  
Phylogenetic Position ◽  
Phylogenetic Study ◽  
Taxon Sampling ◽  
Molecular Phylogenetic Study ◽  
Molecular Phylogenetic ◽  
Cpdna Sequence

A molecular phylogenetic study was conducted to determine the position of the Philippine endemic Miliusa vidalii in the tribe Miliuseae of family Annonaceae utilizing the combined cpDNA sequence data (trnL-F-matK-ndhF-psbA-trnH-ycf1). Bayesian inference and Maximum parsimony analyses revealed a robustly supported monophyletic Miliusa clade (PP=1.00; BS=93%). Miliusa vidalii nested within clade D (PP=1.00; BS=85%) shares similar features by having terminal inflorescences and a notably long pedicel. M. vidalii is resolved as sister to M. lanceolata, which shares morphological features such as length of pedicels, number of secondary veins, shape of leaf apex, and apiculate monocarp. This present study revealed that M. vidalii possesses a conspicuous glandular structure in the inner petals, a feature that is common only to species found in clade A. Therefore, increased taxon sampling is necessary to construct a stronger phylogenetic relationship within the genus.

CIRCUMSCRIPTION AND PHYLOGENETIC RELATIONSHIPS OF GYMNOCARPOS (CARYOPHYLLACEAE-PARONYCHIOIDEAE)

2002 ◽  
Vol 59 (2) ◽  
pp. 221-237 ◽  
Author(s):  
B. OXELMAN ◽  
B. AHLGREN ◽  
M. THULIN
Keyword(s):  
Phylogenetic Relationships ◽  
Internal Transcribed Spacer ◽  
Sister Group ◽  
Morphological Characters ◽  
Phylogenetic Study ◽  
Molecular Phylogenetic Study ◽  
The Status ◽  
Molecular Phylogenetic ◽  
Internal Transcribed Spacer Sequences

A molecular phylogenetic study based on nuclear ribosomal internal transcribed spacer sequences and plastid rps16 intron sequences on the status of the genus Gymnocarpos (Paronychioideae, Caryophyllaceae) is presented. Gymnocarpos decandrus and Sclerocephalus arabicus form a strongly supported clade within a well-supported group consisting also of G. przewalskii, and the rest of Gymnocarpos. Re-examination of morphological characters also supports this conclusion. Paronychia is found to be polyphyletic, with the subgenera Paronychia and Siphonychia forming a strongly supported sister group to Gymnocarpos, whereas P. kapela and P. chlorothyrsa (representing subgen. Anoplonychia) are found to be related to Herniaria and Philippiella. A key to the 10 species recognized in Gymnocarpos is presented, as well as synoptical information on nomenclature and distributions.

scholarly journals Phylogenetic study of endophytic fungi associated with Centella asiatica from Bengkulu and Malaysian accessions based on the ITS rDNA sequence

2019 ◽  
Vol 20 (5) ◽  
Author(s):  
NANI RADIASTUTI ◽  
DWI NINGSIH SUSILOWATI ◽  
HUSHSHILA A BAHALWAN
Keyword(s):  
Phylogenetic Analysis ◽  
Medicinal Plants ◽  
Endophytic Fungi ◽  
Fungal Endophytes ◽  
Centella Asiatica ◽  
Rdna Sequence ◽  
Its Rdna ◽  
Molecular Phylogenetic Analysis ◽  
Phylogenetic Study ◽  
Molecular Phylogenetic

Abstract. Radiastuti N, Bahalwan HA, Susilowati DN. 2019. Phylogenetic study of endophytic fungi associated with Centella asiatica from Bengkulu and Malaysian accessions based on the ITS rDNA sequence. Biodiversitas 20:  1248-1258. Centella asiatica is one of the medicinal plants which is known to be symbiotic with various endophytic fungi. The purpose of this research was to determine diversity of culturable fungal endophyte from C. asiatica. Identification was conducted using molecular phylogenetic analysis based on the ITS rDNA sequence. The result showed that obtained 145 isolates endophytic fungi (from stolons, leaves, roots, petioles) were grouped into 18 morphotaxa (Bengkulu) and 23 morphotaxa (Malaysian). The fungal endophytic were identified as Aspergillus austroafricanus, Aspergillus oryzae, Acrocalymma vagum, Ceratobasidium cornigerum, Ceratobasidium sp., Chaetomium globosum, Colletotrichum karstii, C. gigasporium, C. tabaci, Colletotrichum siamense, Eutypella sp., Fusarium solani, F. oxysporum, F. falciforme, F. keratoplasticum, F. striatum, Fusarium sp., Penicillium capsulatum, Phoma multirostrata, Perenniporia tephropora, Perenniporia sp., Phanerochaete chrysosporium, Phanerochaete stereoides, Phyllosticta capitalensis, Phomopsis asparagi, Peroneutypa scoparia, Phialemoniopsis sp., Mycochaetophora gentinae, Talaromyces sp., Earliella scabrosa, and Trichaptum sp. based on molecular phylogenetic analysis. Fusarium (Nectriaceae) were the most found of fungal endophytes in both C. asiatica Bengkulu and Malaysian accession. The majority strain are including Ascomycetes and Basidiomycetes. The identification of endophytic fungi from medicinal plants is needed as a preliminary study to determine the potential of endophytic fungi producing bioactive compounds.

Description of Ditylenchus acantholimonis n. sp. (Rhabditida: Anguinidae) from Iran, a morphological and molecular phylogenetic study

Nematology ◽  
2021 ◽  
pp. 1-10
Author(s):  
Samira Aliverdi ◽  
Ebrahim Pourjam ◽  
Majid Pedram
Keyword(s):  
New Species ◽  
Rhizosphere Soil ◽  
Phylogenetic Analyses ◽  
Phylogenetic Study ◽  
Pharyngeal Bulb ◽  
Lateral Field ◽  
Molecular Phylogenetic Study ◽  
Molecular Phylogenetic ◽  
Golestan Province ◽  
Tail Tip

Summary Ditylenchus acantholimonis n. sp. is described based on morphological, morphometric and molecular characters. It was isolated from the rhizosphere soil of Acantholimon sp. in Golestan province, Iran, and is mainly characterised by having four lines in the lateral field, a pyriform to bottle-shaped offset pharyngeal bulb, post-vulval uterine sac 36.6-56.1% of the vulva to anus distance long, and a subcylindrical to conical tail with widely rounded tip. It is further characterised by short to medium-sized females, 480-617 μm long, with a fine stylet having small rounded knobs, V = 80.8-83.6, c = 11.0-13.8, c′ = 3.3-4.6, and males with 16.0-17.0 μm long spicules. The new species was morphologically compared with six species having four lines in their lateral field, rounded tail tip and comparable morphometric data namely: D. dipsacoideus, D. emus, D. exilis, D. paraparvus, D. sturhani, and D. solani. It was also compared with two species, D. ferepolitor and D. angustus, forming a maximally supported clade in the 18S tree. The phylogenetic analyses using the maximal number of Anguinidae and several Sphaerularioidea genera based upon partial 18S and 28S rDNA D2-D3 sequences revealed that Ditylenchus is polyphyletic. In the 18S tree, the new species formed a clade with D. ferepolitor (KJ636374) and D. angustus (AJ966483); in the 28S tree it formed a poorly supported clade with D. phyllobios (KT192618) and Ditylenchus sp. (MG865719).

scholarly journals A molecular phylogenetic study of the caecal fluke of poultry, Postharmostomum commutatum (= P. gallinum) (Trematoda: Brachylaimidae)

2018 ◽  
Vol 117 (12) ◽  
pp. 3927-3934 ◽  
Author(s):  
Marisa C. Valadão ◽  
Beatriz C. M. Silva ◽  
Danimar López-Hernández ◽  
Jackson V. Araújo ◽  
Sean A. Locke ◽  
...  
Keyword(s):  
Phylogenetic Study ◽  
Molecular Phylogenetic Study ◽  
Molecular Phylogenetic
Sign in / Sign up

Export Citation Format

Share Document