Molecular data on Phyllodistomum macrocotyle (Digenea: Gorgoderidae) from an intermediate host Dreissena polymorpha (Bivalvia: Dreissenidae) in the Northern Dvina River Basin, Northwest Russia

2021 ◽  
Vol 39 ◽  
pp. 69-75
Author(s):  
Oksana V. Travina ◽  
Yulia V. Bespalaya ◽  
Alexander V. Kondakov ◽  
Olga V. Aksenova ◽  
Irina S. Khrebtova ◽  
...  

We report the first molecular identification of Phyllodistomum macrocotyle (Digenea: Gorgoderidae) from the Northern Dvina River Basin, Northwest Russia. Comparative analysis of our sequence was 100% identical to the 28S and ITS2 rDNA sequences of the species P. macrocotyle in zebra mussels discovered in Poland (Wigry Lake), Lithuania (Elektrenai Reservoir and Sirvinta River), Russia, Belarus (Lepelskoe Lake and Lukomskoe Lake), and in fish Leuciscus idus, Scardinius erythrophthalmus in Russia (Rybinsk Reservoir on the Volga River). At the same time, some sequences of the 28S rDNA of P. macrocotyle in zebra mussel detected in Lithuania, and Belarus were different from our sequence on 0.2-0.6%. The ITS2 sequences which have 100% similarity with our specimens of P. macrocotyle were discovered in sporocyst of phyllodistomes from its intermediate host D. polymorpha in Poland, Lithuania, Russia, and Belarus. Our observation in the Yuras and Isakogorka rivers represents the northernmost record of this host-specific parasite, to the best of our knowledge.

Author(s):  
B. Korzhenevsky ◽  
Gleb Tolkachev ◽  
Nikolay Kolomiycev

The problems of modern geological ecology associated with the study of pollution of sediments of water bodies by heavy metals are considered. The Volga River basin is quite heterogeneous, both in geomorphological and hydrological terms, and in thechnogenical development and usage. A fourrank taxonomy is presented for the selection of sites for monitoring, based on a combination of natural, landscape, climatic and thechnogenical factors. To the largest – the highest taxon – sites of the Ist category – bowls of reservoirs with the slopes and the urban zones, industrial and agricultural structures located within them are carried. Within these areas are allocated to smaller taxa, areas category IInd are the industrial and urban zones, areas category IIIrd are the small rivers without significant contamination and areas category IVth to conduct special observations. The examples of special observations in the study of the annual migration of heavy metals in the system «bottom sediments – water column» on the Ivankovo reservoir are highlighted. The investigations were carried out under the conditions of the standard flow rate for this reservoir and in the conditions of slow water exchange.


2021 ◽  
Vol 95 ◽  
Author(s):  
A. Rana ◽  
A.H. Bhat ◽  
A.K. Chaubey ◽  
V. Půža ◽  
J. Abolafia

Abstract A population of a nematode species belonging to the genus Oscheius was isolated in western Uttar Pradesh, India. Morphological and morphometrical studies on this species showed its high similarity with six species described previously from Pakistan (Oscheius citri, O. cobbi, O. cynodonti, O. esculentus, O. punctatus and O. sacchari). The molecular analysis of the ITS1-5.8S-ITS2 rDNA sequences of the Indian population and the six species described from Pakistan showed that all the sequences are almost identical. Thus, based on morphological and molecular characteristics, all of the six above-mentioned Pakistani species and Indian strain do not differ from each other, hence can be considered synonyms. The correct name for this taxon is the first described species O. citri. Additionally, the phylogenetic analysis of the 18S rDNA and the 28S rDNA sequences showed that Oscheius citri is sister to the clade formed by O. chongmingensis and O. rugaoensis from China. The high similarity of morphological and morphometric characteristics of O. citri and other species, O. maqbooli, O. nadarajani, O. niazii, O. shamimi and O. siddiqii, suggest their conspecificity; however, lack of molecular data for these species does not allow this hypothesis to be tested.


2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Renan Rodrigues Rocha ◽  
Rosana de Mesquita Alves ◽  
Rubens Pasa ◽  
Karine Frehner Kavalco

The Astyanax scabripinnis complex is composed of a large number of almost morphological indistinguishable species, including Astyanax paranae and Astyanax rivularis, which exist in the Paraná and São Francisco Basins, respectively, and sometimes are considered subspecies of the A. scabripinnis group or even are cited just as A. scabripinnis. The two river basins are separated by the Upper Paranaíba Arc, likely the main cause of the isolation of these species. We used geometric morphometric tools and DNA analyses of populations of both species to identify the differences between them. Geometric morphometrics separated the two species into distinct groups, whose main difference was the body depth. This is generally related to the speed of the water flow in the river basins. The maximum likelihood phylogram based on mitochondrial DNA sequences formed two main clades: one composed of the population of A. rivularis and the other, of A. paranae. In the haplotype network, the species were similarly separated into two groups from the same ancestral haplotype, with A. rivularis dispersing into two lineages in the São Francisco River Basin. The distribution of A. paranae is a consequence of a secondary dispersion event in the Paraná River Basin. It forms two lineages from a haplotype derived from the ancestor. The vicariant effect of separate basins, through the elevation of the Upper Paranaíba Arc, led to the allopatric speciation of the populations originating the present species. The results of geometric morphometrics and molecular data of the fish show the importance of this geological event in the biogeography and evolutionary history of the ichthyofauna of the region and indicate that the isolation of these species seems to be effective.


Chemosphere ◽  
2017 ◽  
Vol 168 ◽  
pp. 825-831 ◽  
Author(s):  
Wen-Li Tang ◽  
Douglas Evans ◽  
Lisa Kraemer ◽  
Huan Zhong

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