scholarly journals Direct Analysis of Single Cells by Mass Spectrometry at Atmospheric Pressure

10.3791/2144 ◽  
2010 ◽  
Author(s):  
Bindesh Shrestha ◽  
Akos Vertes
Keyword(s):  
Mass Spectrometry ◽  
Atmospheric Pressure ◽  
Single Cells ◽  
Direct Analysis

scholarly journals Atmospheric Pressure Dark-Current Argon Discharge Ionization with Comparable Performance to Direct Analysis in Real Time Mass Spectrometry

2019 ◽  
Vol 8 (1) ◽  
pp. A0075-A0075
Author(s):  
Kanako Sekimoto ◽  
Motoshi Sakakura ◽  
Hiroshi Hike ◽  
Takatomo Kawamukai ◽  
Teruhisa Shiota ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Real Time ◽  
Atmospheric Pressure ◽  
Dark Current ◽  
Direct Analysis ◽  
Argon Discharge ◽  
Comparable Performance

scholarly journals Ultra-high sensitivity mass spectrometry quantifies single-cell proteome changes upon perturbation

2020 ◽  
Author(s):  
Andreas-David Brunner ◽  
Marvin Thielert ◽  
Catherine Vasilopoulou ◽  
Constantin Ammar ◽  
Fabian Coscia ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Single Cells ◽  
High Sensitivity ◽  
Direct Analysis ◽  
Low Flow ◽  
Isolated Cells ◽  
Chemical Labeling ◽  
Core Proteome ◽  
Proteome Changes

AbstractSingle-cell technologies are revolutionizing biology but are today mainly limited to imaging and deep sequencing. However, proteins are the main drivers of cellular function and in-depth characterization of individual cells by mass spectrometry (MS)-based proteomics would thus be highly valuable and complementary. Chemical labeling-based single-cell approaches introduce hundreds of cells into the MS, but direct analysis of single cells has not yet reached the necessary sensitivity, robustness and quantitative accuracy to answer biological questions. Here, we develop a robust workflow combining miniaturized sample preparation, very-low flow-rate chromatography and a novel trapped ion mobility mass spectrometer, resulting in a more than ten-fold improved sensitivity. We accurately and robustly quantify proteomes and their changes in single, FACS-isolated cells. Arresting cells at defined stages of the cell cycle by drug treatment retrieves expected key regulators such as CDK2NA, E2 ubiquitin ligases such as UBE2S and highlights potential novel ones. Comparing the variability in more than 420 single-cell proteomes to transcriptome data revealed a stable core proteome despite perturbation. Our technology can readily be applied to ultra-high sensitivity analysis of tissue material, including post-translational modifications and to small molecule studies.

Direct Analysis of Carbonyl Compounds by Mass Spectrometry with Double-Region Atmospheric Pressure Chemical Ionization

2019 ◽  
Vol 91 (9) ◽  
pp. 5715-5721 ◽  
Author(s):  
Yihan Zhang ◽  
Wuduo Zhao ◽  
Dingzhong Wang ◽  
Hongtu Zhang ◽  
Guobi Chai ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Atmospheric Pressure ◽  
Carbonyl Compounds ◽  
Chemical Ionization ◽  
Atmospheric Pressure Chemical Ionization ◽  
Direct Analysis ◽  
Pressure Chemical Ionization ◽  
Pressure Chemical
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