scholarly journals Procedures for Rat in situ Skeletal Muscle Contractile Properties

10.3791/3167 ◽  
2011 ◽  
Author(s):  
Brian R. MacIntosh ◽  
Shane P. Esau ◽  
R. John Holash ◽  
Jared R. Fletcher
Keyword(s):  
Skeletal Muscle ◽  
Contractile Properties ◽  
Muscle Contractile Properties ◽  
Muscle Contractile

Effect of caffeine on skeletal muscle function before and after fatigue

1983 ◽  
Vol 54 (5) ◽  
pp. 1303-1305 ◽  
Author(s):  
J. M. Lopes ◽  
M. Aubier ◽  
J. Jardim ◽  
J. V. Aranda ◽  
P. T. Macklem
Keyword(s):  
Skeletal Muscle ◽  
Electrical Stimulation ◽  
Relaxation Rate ◽  
Voluntary Contraction ◽  
Contractile Properties ◽  
Double Blind ◽  
Caffeine Ingestion ◽  
Muscle Contractile Properties ◽  
Before And After ◽  
Muscle Contractile

We studied the effect of caffeine on voluntary and electrically stimulated contractions of the adductor pollicis muscle in five adult volunteers. Caffeine (500 mg) was administered orally in a double-blind fashion. Electrical stimulation of the ulnar nerve was performed at 10, 20, 30, 50, and 100 Hz before and after a sustained voluntary contraction held at 50% of the maximal voluntary contraction (MVC). A brief tetanus at 30 Hz was also performed to calculate relaxation rate in the fresh muscle. Contractile properties, relaxation rate, and endurance were then assessed after caffeine and placebo, as well as the response of the fatigued muscle to different frequencies of stimulation. There was no difference in the maximal tension obtained with electrical stimulation (T100) or in the MVC between placebo and caffeine. The tensions developed with electrical stimulation at lower frequencies increased significantly with caffeine ingestion, shifting the frequency-force curve to the left, both before and after fatigue. Mean plasma caffeine concentration associated with these responses was 12.2 +/- 4.9 mg/l. We conclude that caffeine has a direct effect on skeletal muscle contractile properties both before and after fatigue as demonstrated by electrical stimulation.

Effect of Cold-Water Immersion on Skeletal Muscle Contractile Properties in Soccer Players

2011 ◽  
Vol 90 (5) ◽  
pp. 356-363 ◽  
Author(s):  
Juan Manuel García-Manso ◽  
Darío Rodríguez-Matoso ◽  
David Rodríguez-Ruiz ◽  
Samuel Sarmiento ◽  
Yves de Saa ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cold Water ◽  
Water Immersion ◽  
Cold Water Immersion ◽  
Contractile Properties ◽  
Soccer Players ◽  
Muscle Contractile Properties ◽  
Muscle Contractile

Muscle contractile properties during intermittent nontetanic stimulation in rat skeletal muscle

2001 ◽  
Vol 281 (6) ◽  
pp. R1952-R1965 ◽  
Author(s):  
Esther Verburg ◽  
Hanne-Mari Schiøtz Thorud ◽  
Morten Eriksen ◽  
Nina K. Vøllestad ◽  
Ole M. Sejersted
Keyword(s):  
Skeletal Muscle ◽  
Relaxation Time ◽  
Low Frequency ◽  
Creatine Phosphate ◽  
Contractile Properties ◽  
Second Phase ◽  
Phase Relaxation ◽  
Two Phase ◽  
Muscle Contractile

To examine changes in contractile properties and mechanisms of fatigue during submaximal nontetanic skeletal muscle activity, in situ perfused soleus (60-min protocol) and extensor digitorum longus (EDL; 10-min protocol) muscles of the rat were electrically stimulated intermittently at low frequency. The partly fused trains of contractions showed a two-phase change in appearance. During the first phase, relaxation slowed, one-half relaxation time increased, and maximal relaxation first derivative of force (dF/d t) decreased. Developed force during the trains was reduced and was closely related to the rate of relaxation in this first phase. During the second phase, relaxation became faster again, one-half relaxation time decreased, and force returned to resting levels between contractions in a train. In contrast, developed force remained reduced, so that peak force of the contractions was 51% (soleus) and 30% (EDL) of control. In the soleus muscle, the changes in contractile properties were not related to ATP, creatine phosphate, or lactate content. The changes in contractile properties fit best with a mechanism of fatigue involving changes in Ca2+ handling by the sarcoplasmic reticulum.

scholarly journals Peanut protein reduces body protein mass and alters skeletal muscle contractile properties and lipid metabolism in rats

2009 ◽  
Vol 103 (9) ◽  
pp. 1331-1339 ◽  
Author(s):  
Hélène Jacques ◽  
Nadine Leblanc ◽  
Roxanne Papineau ◽  
Denis Richard ◽  
Claude H. Côté
Keyword(s):  
Skeletal Muscle ◽  
Body Composition ◽  
Lipid Metabolism ◽  
Liver Weight ◽  
Peanut Protein ◽  
Contractile Properties ◽  
Body Protein ◽  
Protein Mass ◽  
Muscle Contractile Properties ◽  
Muscle Contractile

It is well known that diets high in nuts or peanuts favourably affect plasma lipid concentrations. However, few studies have examined the effects of nut and peanut protein (PP) on body composition and skeletal muscle properties. The present study was aimed at evaluating the effect of dietary PP compared with two animal proteins, casein (C) and cod protein (CP) on body composition, skeletal muscle contractile properties and lipid metabolism in rats. Thirty-two male rats were assigned to one of the following four diets containing either C, CP, PP or C+peanut protein (CPP, 50:50) mixture. After 28 d of ad libitum feeding and after 12-h fast, blood, liver and muscle were collected for measurements of plasma and hepatic cholesterol and TAG, plasma glucose and insulin and contractile properties. Rats fed with the low-quality protein, PP, had lower body weight gain, body protein mass, soleus mass and liver weight than those fed with the high-quality dietary proteins, C and CP. PP also caused a deficit in contractile properties in soleus. Likewise, PP increased plasma cholesterol and body fat mass compared with CP. However, these elevations were accompanied with increased hepatic TAG concentrations and lowered intestinal fat excretion. These results show that PP intake alters body composition by reducing skeletal muscle mass and liver weight as well as muscle contractility and lipid metabolism. Adding a complete protein such as C might partially counteract these adverse effects.

scholarly journals Vitamin D supplementation does not improve human skeletal muscle contractile properties in insufficient young males

2014 ◽  
Vol 114 (6) ◽  
pp. 1309-1320 ◽  
Author(s):  
Daniel J. Owens ◽  
Daniel Webber ◽  
Samuel G. Impey ◽  
Jonathan Tang ◽  
Timothy F. Donovan ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Vitamin D ◽  
Human Skeletal Muscle ◽  
Vitamin D Supplementation ◽  
Contractile Properties ◽  
Young Males ◽  
Muscle Contractile Properties ◽  
Muscle Contractile

Effects of N-acetylcysteine on isolated mouse skeletal muscle: contractile properties, temperature dependence, and metabolism

2013 ◽  
Vol 466 (3) ◽  
pp. 577-585 ◽  
Author(s):  
Abram Katz ◽  
Andrés Hernández ◽  
Diana Marcela Ramos Caballero ◽  
Javier Fernando Bonilla Briceno ◽  
Laura Victoria Rivera Amezquita ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Temperature Dependence ◽  
Contractile Properties ◽  
Mouse Skeletal Muscle ◽  
Muscle Contractile Properties ◽  
Muscle Contractile
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