scholarly journals Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes

10.3791/55743 ◽  
2017 ◽  
Author(s):  
Stephanie Chanon ◽  
Christine Durand ◽  
Aurelie Vieille-Marchiset ◽  
Maud Robert ◽  
Charna Dibner ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Insulin Stimulation ◽  
Uptake Measurement

scholarly journals Specialized sorting of GLUT4 and its recruitment to the cell surface are independently regulated by distinct Rabs

2013 ◽  
Vol 24 (16) ◽  
pp. 2544-2557 ◽  
Author(s):  
L. Amanda Sadacca ◽  
Joanne Bruno ◽  
Jennifer Wen ◽  
Wenyong Xiong ◽  
Timothy E. McGraw
Keyword(s):  
Plasma Membrane ◽  
Glucose Uptake ◽  
Glucose Transporter ◽  
Receptor Activation ◽  
Glut4 Translocation ◽  
Insulin Stimulation ◽  
Transport Vesicles ◽  
Glucose Transporter Glut4

Adipocyte glucose uptake in response to insulin is essential for physiological glucose homeostasis: stimulation of adipocytes with insulin results in insertion of the glucose transporter GLUT4 into the plasma membrane and subsequent glucose uptake. Here we establish that RAB10 and RAB14 are key regulators of GLUT4 trafficking that function at independent, sequential steps of GLUT4 translocation. RAB14 functions upstream of RAB10 in the sorting of GLUT4 to the specialized transport vesicles that ferry GLUT4 to the plasma membrane. RAB10 and its GTPase-activating protein (GAP) AS160 comprise the principal signaling module downstream of insulin receptor activation that regulates the accumulation of GLUT4 transport vesicles at the plasma membrane. Although both RAB10 and RAB14 are regulated by the GAP activity of AS160 in vitro, only RAB10 is under the control of AS160 in vivo. Insulin regulation of the pool of RAB10 required for GLUT4 translocation occurs through regulation of AS160, since activation of RAB10 by DENND4C, its GTP exchange factor, does not require insulin stimulation.

scholarly journals X-ray Fluorescence Uptake Measurement of Functionalized Gold Nanoparticles in Tumor Cell Microsamples

2021 ◽  
Vol 22 (7) ◽  
pp. 3691
Author(s):  
Oliver Schmutzler ◽  
Sebastian Graf ◽  
Nils Behm ◽  
Wael Y. Mansour ◽  
Florian Blumendorf ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Irradiation Time ◽  
High Sensitivity ◽  
Nanoparticle Uptake ◽  
X Ray ◽  
Functionalized Gold Nanoparticles ◽  
Uptake Measurement ◽  
Prostate Tumor Cells ◽  
Non Destructive

Quantitative cellular in vitro nanoparticle uptake measurements are possible with a large number of different techniques, however, all have their respective restrictions. Here, we demonstrate the application of synchrotron-based X-ray fluorescence imaging (XFI) on prostate tumor cells, which have internalized differently functionalized gold nanoparticles. Total nanoparticle uptake on the order of a few hundred picograms could be conveniently observed with microsamples consisting of only a few hundreds of cells. A comparison with mass spectroscopy quantification is provided, experimental results are both supported and sensitivity limits of this XFI approach extrapolated by Monte-Carlo simulations, yielding a minimum detectable nanoparticle mass of just 5 pg. This study demonstrates the high sensitivity level of XFI, allowing non-destructive uptake measurements with very small microsamples within just seconds of irradiation time.

In vitro glucose uptake activity of Aegles marmelos and Syzygium cumini by activation of Glut-4, PI3 kinase and PPARγ in L6 myotubes

Phytomedicine ◽  
2006 ◽  
Vol 13 (6) ◽  
pp. 434-441 ◽  
Author(s):  
R. Anandharajan ◽  
S. Jaiganesh ◽  
N.P. Shankernarayanan ◽  
R.A. Viswakarma ◽  
A. Balakrishnan
Keyword(s):  
Glucose Uptake ◽  
Pi3 Kinase ◽  
Syzygium Cumini ◽  
Glut 4 ◽  
L6 Myotubes ◽  
Uptake Activity

scholarly journals Methanolic leaf extract of Gymnema sylvestre augments glucose uptake and ameliorates Insulin resistance by upregulating GLUT-4, PPAR- and #947;, adiponectin and leptin levels in vitro

2016 ◽  
Vol 5 (2) ◽  
pp. 146 ◽  
Author(s):  
Puttanarasaiah Kumar ◽  
Marikunte Venkataranganna ◽  
Kirangadur Manjunath ◽  
Gollapalle Viswanatha ◽  
Godavarthi Ashok
Keyword(s):  
Insulin Resistance ◽  
Glucose Uptake ◽  
Leaf Extract ◽  
Gymnema Sylvestre ◽  
Glut 4

Contraction-related stimuli regulate GLUT4 traffic in C2C12-GLUT4myc skeletal muscle cells

2010 ◽  
Vol 298 (5) ◽  
pp. E1058-E1071 ◽  
Author(s):  
Wenyan Niu ◽  
Philip J. Bilan ◽  
Shuhei Ishikura ◽  
Jonathan D. Schertzer ◽  
Ariel Contreras-Ferrat ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cell Surface ◽  
Glucose Uptake ◽  
Nicotinic Acetylcholine Receptors ◽  
Cell Model ◽  
Cytosolic Calcium ◽  
Atpase Inhibitor ◽  
Cellular Models ◽  
Compound C

Muscle contraction stimulates glucose uptake acutely to increase energy supply, but suitable cellular models that faithfully reproduce this complex phenomenon are lacking. To this end, we have developed a cellular model of contracting C2C12 myotubes overexpressing GLUT4 with an exofacial myc-epitope tag (GLUT4 myc) and explored stimulation of GLUT4 traffic by physiologically relevant agents. Carbachol (an acetylcholine receptor agonist) induced a gain in cell surface GLUT4 myc that was mediated by nicotinic acetylcholine receptors. Carbachol also activated AMPK, and this response was sensitive to the contractile myosin ATPase inhibitor N-benzyl- p-toluenesulfonamide. The gain in surface GLUT4 myc elicited by carbachol or by the AMPK activator 5-amino-4-carboxamide-1 β-ribose was sensitive to chemical inhibition of AMPK activity by compound C and partially reduced by siRNA-mediated knockdown of AMPK catalytic subunits or LKB1. In addition, the carbachol-induced gain in cell surface GLUT4 myc was partially sensitive to chelation of intracellular calcium with BAPTA-AM. However, the carbachol-induced gain in cell surface GLUT4 myc was not sensitive to the CaMKK inhibitor STO-609 despite expression of both isoforms of this enzyme and a rise in cytosolic calcium by carbachol. Therefore, separate AMPK- and calcium-dependent signals contribute to mobilizing GLUT4 in response to carbachol, providing an in vitro cell model that recapitulates the two major signals whereby acute contraction regulates glucose uptake in skeletal muscle. This system will be ideal to further analyze the underlying molecular events of contraction-regulated GLUT4 traffic.

Role of α1A-adrenoceptor in the regulation of glucose uptake into white adipocyte of rats in vitro

2000 ◽  
Vol 84 (3) ◽  
pp. 140-146 ◽  
Author(s):  
Juei-Tang Cheng ◽  
I-Min Liu ◽  
Shi-Ting Yen ◽  
Pei-Chi Chen
Keyword(s):  
Glucose Uptake ◽  
White Adipocyte

Prolonged hyperglycemia in vitro affects glucose transporter protein gluti and glucose uptake in cultured term trophoblast cells

Placenta ◽  
1994 ◽  
Vol 15 (7) ◽  
pp. A4 ◽  
Author(s):  
S. Barth ◽  
T. Hahn ◽  
R. Zechner ◽  
G. Desoye
Keyword(s):  
Glucose Uptake ◽  
Glucose Transporter ◽  
Trophoblast Cells ◽  
Transporter Protein

Kinetics of uptake of glucose in rabbit jejunum: influence of sodium, unstirred layers, and passive permeation

1983 ◽  
Vol 61 (10) ◽  
pp. 1129-1137 ◽  
Author(s):  
A. B. R. Thomson
Keyword(s):  
Glucose Uptake ◽  
Intestinal Transport ◽  
Water Layer ◽  
Lauryl Alcohol ◽  
Unstirred Layer ◽  
Transport Rate ◽  
Rabbit Jejunum ◽  
Layer Resistance ◽  
Kinetics Of Uptake

Failure to account for the effect of the unstirred water layer and the contribution of passive permeation will lead to errors in the estimation of the kinetic constants of glucose uptake into the intestine. It is widely accepted that variations in the concentration of sodium in the bulk phase profoundly influence the rate of uptake of glucose in the intestine, but the kinetic basis for this effect remains in dispute. Accordingly, a previously validated in vitro technique was used to assess the effect of Na+ on the uptake of glucose into rabbit jejunum under conditions selected to reduce the unstirred layer resistance. Varying Na+ had no effect on the uptake of lauryl alcohol and therefore on unstirred layer resistance. The passive permeability coefficient for glucose uptake was estimated from the uptake of L-glucose, of D-glucose at 4 °C, or in the presence of 1 mM phlorizin or 40 mM galactose. The permeability for glucose increased as Na+ rose. The values of both the maximal transport rate and the Michaelis constant (Km) were influenced by Na+. A linear relationship was noted between Na+ and the maximal transport rate; the value of Km fell as Na+ was increased to 75 mequiv./L, but Km did not decline further with higher values of Na+. These results support the theoretical predictions of the presence of both an affinity and a velocity effect of the sodium gradient on the intestinal transport system for glucose.

scholarly journals Interleukin-6 Increases Insulin-Stimulated Glucose Disposal in Humans and Glucose Uptake and Fatty Acid Oxidation In Vitro via AMP-Activated Protein Kinase

Diabetes ◽  
2006 ◽  
Vol 55 (10) ◽  
pp. 2688-2697 ◽  
Author(s):  
A. L. Carey ◽  
G. R. Steinberg ◽  
S. L. Macaulay ◽  
W. G. Thomas ◽  
A. G. Holmes ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Protein Kinase ◽  
Glucose Uptake ◽  
Fatty Acid Oxidation ◽  
Interleukin 6 ◽  
Glucose Disposal ◽  
Acid Oxidation ◽  
Amp Activated Protein Kinase
Sign in / Sign up

Export Citation Format

Share Document