Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

10.3791/59549 ◽  
2019 ◽  
Author(s):  
John Hendershot ◽  
Adam E. Smith ◽  
Thomas A. Werfel
Keyword(s):  
Polymeric Nanoparticles ◽  
Sirna Delivery ◽  
Ph Responsive

scholarly journals Rhein laden pH-responsive polymeric nanoparticles for treatment of osteoarthritis

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Bo Hu ◽  
Feng Gao ◽  
Chunbao Li ◽  
Boqing Zhang ◽  
Mingyang An ◽  
...  
Keyword(s):  
Polymeric Nanoparticles ◽  
Ph Responsive

scholarly journals Smart Polymeric Nanoparticles with pH-Responsive and PEG-Detachable Properties (II): Co-Delivery of Paclitaxel and VEGF siRNA for Synergistic Breast Cancer Therapy in Mice

2021 ◽  
Vol Volume 16 ◽  
pp. 5479-5494
Author(s):  
Mingji Jin ◽  
Yan Hou ◽  
Xiuquan Quan ◽  
Liqing Chen ◽  
Zhonggao Gao ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Therapy ◽  
Polymeric Nanoparticles ◽  
Ph Responsive ◽  
Breast Cancer Therapy

pH-responsive hybrid quantum dots for targeting hypoxic tumor siRNA delivery

2015 ◽  
Vol 220 ◽  
pp. 529-544 ◽  
Author(s):  
HongYan Zhu ◽  
ShengYu Zhang ◽  
Yong Ling ◽  
GuoLiang Meng ◽  
Yu Yang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Sirna Delivery ◽  
Ph Responsive ◽  
Hypoxic Tumor

scholarly journals Breathing Life into Polycations:  Functionalization with pH-Responsive Endosomolytic Peptides and Polyethylene Glycol Enables siRNA Delivery

2008 ◽  
Vol 130 (11) ◽  
pp. 3272-3273 ◽  
Author(s):  
Martin Meyer ◽  
Alexander Philipp ◽  
Reza Oskuee ◽  
Claudia Schmidt ◽  
Ernst Wagner
Keyword(s):  
Polyethylene Glycol ◽  
Sirna Delivery ◽  
Ph Responsive

Water-Soluble, pH Responsive Polymeric Nanoparticles: A Modular Approach

2020 ◽  
Vol 2 (7) ◽  
pp. 2499-2503
Author(s):  
Marcel Klein-Hitpaß ◽  
Jan-Erik Ostwaldt ◽  
Carsten Schmuck ◽  
Michael Giese
Keyword(s):  
Polymeric Nanoparticles ◽  
Water Soluble ◽  
Modular Approach ◽  
Ph Responsive

scholarly journals Strontium Sulfite: A New pH-Responsive Inorganic Nanocarrier to Deliver Therapeutic siRNAs to Cancer Cells

Pharmaceutics ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 89 ◽  
Author(s):  
Md. Karim ◽  
Jayalaxmi Shetty ◽  
Rowshan Islam ◽  
Ahsanul Kaiser ◽  
Athirah Bakhtiar ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Tumor Regression ◽  
Sirna Delivery ◽  
Particle Growth ◽  
Inorganic Nanoparticles ◽  
Ph Responsive ◽  
Growth Inhibition Assay ◽  
Cancer Cell Death ◽  
Biodistribution Studies

Inorganic nanoparticles hold great potential in the area of precision medicine, particularly for treating cancer owing to their unique physicochemical properties, biocompatibility and improved pharmacokinetics properties compared to their organic counterparts. Here we introduce strontium sulfite nanoparticles as new pH-responsive inorganic nanocarriers for efficient transport of siRNAs into breast cancer cells. We employed the simplest nanoprecipitation method to generate the strontium sulfite nanoparticles (SSNs) and demonstrated the dramatic roles of NaCl and d-glucose in particle growth stabilization in order to produce even smaller nanosize particles (Na-Glc-SSN) with high affinity towards negatively charged siRNA, enabling it to efficiently enter the cancer cells. Moreover, the nanoparticles were found to be degraded with a small drop in pH, suggesting their potential capability to undergo rapid dissolution at endosomal pH so as to release the payload. While these particles were found to be nontoxic to the cells, they showed higher potency in facilitating cancer cell death through intracellular delivery and release of oncogene-specific siRNAs targeting ros1 and egfr1 mRNA transcripts, than the strontium sulfite particles prepared in absence of NaCl and d-glucose, as confirmed by growth inhibition assay. The mouse plasma binding analysis by Q-TOF LC-MS/MS demonstrated less protein binding to smaller particles of Na-Glc-SSNs. The biodistribution studies of the particles after 4 h of treatment showed Na-Glc-SSNs had less off-target distribution than SSNs, and after 24 h, all siRNAs were cleared from all major organs except the tumors. ROS1 siRNA with its potential therapeutic role in treating 4T1-induced breast tumor was selected for subsequent in vivo tumor regression study, revealing that ROS1 siRNA-loaded SSNs exerted more significant anti-tumor effects than Na-Glc-SSNs carrying the same siRNA following intravenous administration, without any systemic toxicity. Thus, strontium sulfite emerged as a powerful siRNA delivery tool with potential applications in cancer gene therapy.

Key determinants of siRNA delivery mediated by unique pH-responsive lipid-based liposomes

2019 ◽  
Vol 569 ◽  
pp. 118606 ◽  
Author(s):  
Mariko Sako ◽  
Furan Song ◽  
Ayaka Okamoto ◽  
Hiroyuki Koide ◽  
Takehisa Dewa ◽  
...  
Keyword(s):  
Sirna Delivery ◽  
Ph Responsive

scholarly journals Optimizing Mannose “Click” Conjugation to Polymeric Nanoparticles for Targeted siRNA Delivery to Human and Murine Macrophages

ACS Omega ◽  
2019 ◽  
Vol 4 (16) ◽  
pp. 16756-16767 ◽  
Author(s):  
Evan B. Glass ◽  
Shirin Masjedi ◽  
Stephanie O. Dudzinski ◽  
Andrew J. Wilson ◽  
Craig L. Duvall ◽  
...  
Keyword(s):  
Polymeric Nanoparticles ◽  
Sirna Delivery ◽  
Murine Macrophages

scholarly journals The Effects of Biological Fluids on Colloidal Stability and siRNA Delivery of a pH-Responsive Micellar Nanoparticle Delivery System

ACS Nano ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. 187-197 ◽  
Author(s):  
Dominic W. Malcolm ◽  
Jomy J. Varghese ◽  
Janet E. Sorrells ◽  
Catherine E. Ovitt ◽  
Danielle S. W. Benoit
Keyword(s):  
Delivery System ◽  
Colloidal Stability ◽  
Biological Fluids ◽  
Sirna Delivery ◽  
Ph Responsive ◽  
Nanoparticle Delivery

pH Responsive Polymeric Nanoparticles for Oral Insulin Delivery

2017 ◽  
pp. 6-10
Author(s):  
Charu Tyagi
Keyword(s):  
Insulin Release ◽  
Polymeric Nanoparticles ◽  
Attenuated Total Reflectance ◽  
Ph Responsive ◽  
Eudragit L100 ◽  
Release Studies ◽  
Mean Size ◽  
The Mean ◽  
In Vitro Insulin Release

Gelatin-eudragit L100 nanoparticles of wet size range 170-563nm were prepared by two step dissolvation method and the effect of different concentrations of eudragit L100 and emulsifying agent - sodium lauryl sulphate (SLS) - on the particle size were studied. Synthesized nanoparticles were characterized by attenuated total reflectance-fourier transform infrared spectroscopy (ATRFTIR) and the mean size distribution. Insulin loading was done at a pH 7.4 and the in vitro insulin release studies of nanoparticles were carried out by simulating gastrointestinal tract condition which showed the minimal insulin release at pH 2.5 (20% in 90min) while appreciable release (40% in first 30min) at pH of 7.4. This pH responsive release pattern of the synthesized nanoparticles confers on the insulin protection from proteolytic degradation in acidic environment of stomach and upper intestinal part while enhancing bioavailability in the later part of intestine.

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