High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues

10.3791/61767 ◽  
2021 ◽  
Author(s):  
Ravi Lokwani ◽  
Kaitlyn Sadtler
Keyword(s):  
Flow Cytometry ◽  
Immune Function ◽  
Function Analysis ◽  
High Dimensionality

scholarly journals Effect of Fluoride on Endocytosis and Surface Marker Expression Levels of Mouse B Cells In Vitro

2016 ◽  
Vol 39 (2) ◽  
pp. 596-603 ◽  
Author(s):  
Haili Ma ◽  
Zeyu Shi ◽  
Yaoze Dong ◽  
Rui Liang ◽  
Jianshan Chen ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
B Cells ◽  
Immune Function ◽  
Sodium Fluoride ◽  
Surface Marker ◽  
Expression Levels ◽  
Surface Marker Expression ◽  
Marker Expression ◽  
High Concentrations

Background/Aims: To clarify the effect of fluoride on splenic B cells, the endocytosis and surface marker expression levels of mouse splenic B cells were detected in vitro by flow cytometry. Methods: Cells were stimulated with 10 µg/mL lipopolysaccharide (LPS) and varying concentrations of Sodium Fluoride (NaF) (0, 50 µM, 100 µM, 500 µM, 1000 µM). Results: The results demonstrated that the endocytic capacity of B cells was enhanced by NaF at 50µM. NaF significantly enhanced CD80 expression at 50 µM and decreased CD86 expression at 500 µM. CD40 and CD138 expression on B cells were down-regulated at varying high concentrations of NaF. Conclusion: our results showed that the endocytic capacity, expression levels of CD40 and CD80 of B cells changed significantly at lower concentrations, whereas expression levels of CD138 and CD86 changed significantly at higher concentrations, suggesting that fluoride could inhibit immune function in animals.

Use of Multi-Parameter Flow Cytometry to Determine Cord Blood Innate Immune Function Associated with Prenatal Farming Exposure

2015 ◽  
Vol 135 (2) ◽  
pp. AB149
Author(s):  
Christine M. Seroogy ◽  
Lauren Nettenstrom ◽  
Michael D. Evans ◽  
Matt Keifer ◽  
James E. Gern
Keyword(s):  
Flow Cytometry ◽  
Cord Blood ◽  
Immune Function ◽  
Innate Immune ◽  
Innate Immune Function

scholarly journals In vitro effects of PCDDs/Fs on NK-like cell activity of Eisenia andrei earthworms

2012 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Hayet Belmeskine ◽  
Sami Haddad ◽  
Louise Vandelac ◽  
Michel Fournier
Keyword(s):  
Flow Cytometry ◽  
Immune Function ◽  
Cell Activity ◽  
Eisenia Andrei ◽  
Significant Stimulation ◽  
In Vitro Effects ◽  
Tumoral Cells ◽  
Stimulation Of

In this study, we assessed<em> in vitro</em> the effects of PCDD/Fs on the NK-like cell activity in<em> Eisenia andrei</em> earthworms using flow cytometry for analysis. NK-like coelomocytes isolated from <em>E. andrei</em> and used as effectors were exposed to various concentrations of PCDDs/Fs mixture, C1 (6.25x10-3 ng 2378- TCDD/mL), C2 (12.5x10-3 ng 2378-TCDD/mL) and C3 (25x10-3 ng 2378-TCDD/mL), before adding them to human tumoral cells (K562) used as targets. We evaluated the percentage of targets lysed by Nk-like cells. The results showed a significant stimulation of the NKlike activity at C3 when PCDD/Fs were not removed from effectors before contact with targets, while no effects were noted when the effectors were washed (PCDD/Fs removed) or fixed. Assessment of the viability of the targets (K562), exposed alone and separately from effectors, to the three concentrations of PCDD/Fs, C1, C2 and C3, showed that all these concentrations were cytotoxic for K562. Results suggest that PCDD/Fs concentrations tested in this assay may be considered too low to induce suppressive effects on the immune function such as the NK-like activity in <em>E. andrei</em> earthworms.

scholarly journals Comparative leucocyte populations between two sympatric carnivores (Nasua narica and Procyon lotor)

2019 ◽  
Vol 7 (1) ◽  
Author(s):  
Emilio Rendón-Franco ◽  
Osvaldo López-Díaz ◽  
Oscar Rodríguez-Espinosa ◽  
Nora Rojas-Serranía ◽  
Roberto Rodríguez-Cabo-Mercado ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Immune System ◽  
Immune Function ◽  
Procyon Lotor ◽  
Zoonotic Diseases ◽  
Total Count ◽  
Kinetic Assay ◽  
Phagocytic Capacity ◽  
Blood Samples ◽  
Nasua Narica

Abstract Coatis (Nasua narica) and raccoons (Procyon lotor) potentially play an important role in zoonotic diseases because they may carry pathogens and can transmit them to humans. To date, our understanding of the immune function of these two carnivores is deficient. The aim of this study was to compare the number of leucocyte subtypes and the phagocytic capacity between the coati and the raccoon. Blood samples were collected, and leucocyte subtypes were characterized and counted by flow cytometry and microscopy, respectively. Phagocytosis was analysed by kinetic assay. Differences in leucocytes between these two species were found; the total count of neutrophils was higher in raccoons than in coatis, but lymphocytes and eosinophils were higher in coatis than in raccoons. Antigen reduction was more rapid for the coatis. However, raccoons had a higher efficient endocytic process than coatis. This study provides the basis for understanding the procyonid immune system, which informs conservation, particularly since some procyonids are imperilled.

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