Cytokine flow cytometry: multiparametric approach to immune function analysis

Cytotherapy ◽  
2003 ◽  
Vol 5 (1) ◽  
pp. 1-6 ◽  
Keyword(s):  
Flow Cytometry ◽  
Immune Function ◽  
Function Analysis ◽  
Cytokine Flow Cytometry

scholarly journals Effect of Fluoride on Endocytosis and Surface Marker Expression Levels of Mouse B Cells In Vitro

2016 ◽  
Vol 39 (2) ◽  
pp. 596-603 ◽  
Author(s):  
Haili Ma ◽  
Zeyu Shi ◽  
Yaoze Dong ◽  
Rui Liang ◽  
Jianshan Chen ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
B Cells ◽  
Immune Function ◽  
Sodium Fluoride ◽  
Surface Marker ◽  
Expression Levels ◽  
Surface Marker Expression ◽  
Marker Expression ◽  
High Concentrations

Background/Aims: To clarify the effect of fluoride on splenic B cells, the endocytosis and surface marker expression levels of mouse splenic B cells were detected in vitro by flow cytometry. Methods: Cells were stimulated with 10 µg/mL lipopolysaccharide (LPS) and varying concentrations of Sodium Fluoride (NaF) (0, 50 µM, 100 µM, 500 µM, 1000 µM). Results: The results demonstrated that the endocytic capacity of B cells was enhanced by NaF at 50µM. NaF significantly enhanced CD80 expression at 50 µM and decreased CD86 expression at 500 µM. CD40 and CD138 expression on B cells were down-regulated at varying high concentrations of NaF. Conclusion: our results showed that the endocytic capacity, expression levels of CD40 and CD80 of B cells changed significantly at lower concentrations, whereas expression levels of CD138 and CD86 changed significantly at higher concentrations, suggesting that fluoride could inhibit immune function in animals.

Use of Multi-Parameter Flow Cytometry to Determine Cord Blood Innate Immune Function Associated with Prenatal Farming Exposure

2015 ◽  
Vol 135 (2) ◽  
pp. AB149
Author(s):  
Christine M. Seroogy ◽  
Lauren Nettenstrom ◽  
Michael D. Evans ◽  
Matt Keifer ◽  
James E. Gern
Keyword(s):  
Flow Cytometry ◽  
Cord Blood ◽  
Immune Function ◽  
Innate Immune ◽  
Innate Immune Function
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