scholarly journals Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

10.3791/61802 ◽  
2021 ◽  
Author(s):  
Irini M. Dijkhoff ◽  
Benedetta Petracca ◽  
Roxane Prieux ◽  
Giuseppe Valacchi ◽  
Barbara Rothen-Rutishauser ◽  
...  
Keyword(s):  
Large Scale ◽  
Three Dimensional ◽  
Human Epidermis ◽  
Reconstructed Human Epidermis

scholarly journals In a three-dimensional reconstructed human epidermis filaggrin-2 is essential for proper cornification

2015 ◽  
Vol 6 (2) ◽  
pp. e1656-e1656 ◽  
Author(s):  
V Pendaries ◽  
M Le Lamer ◽  
L Cau ◽  
B Hansmann ◽  
J Malaisse ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Human Epidermis ◽  
Reconstructed Human Epidermis

scholarly journals Development of Epidermal Equivalent from Electrospun Synthetic Polymers for In Vitro Irritation/Corrosion Testing

Nanomaterials ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2528
Author(s):  
Denisse Esther Mallaupoma Camarena ◽  
Larissa Satiko Alcântara Sekimoto Matsuyama ◽  
Silvya Stuchi Maria-Engler ◽  
Luiz Henrique Catalani
Keyword(s):  
Three Dimensional ◽  
Synthetic Polymers ◽  
In Vitro Models ◽  
Human Epidermis ◽  
Polybutylene Terephthalate ◽  
Reconstructed Human Epidermis ◽  
Dermal Irritation ◽  
Thickness Skin ◽  
Cell Growth And Proliferation

The development of products for topical applications requires analyses of their skin effects before they are destined for the market. At present, the ban on animal use in several tests makes the search for in vitro models (such as artificial skin) necessary to characterize the risks involved. In this work, tissue engineering concepts were used to manufacture collagen-free three-dimensional scaffolds for cell growth and proliferation. Two different human skin models—reconstructed human epidermis and full-thickness skin—were developed from electrospun scaffolds using synthetic polymers such as polyethylene terephthalate, polybutylene terephthalate, and nylon 6/6. After the construction of these models, their histology was analyzed by H&E staining and immunohistochemistry. The results revealed a reconstructed epidermal tissue, duly stratified, obtained from the nylon scaffold. In this model, the presence of proteins involved in the epidermis stratification process (cytokeratin 14, cytokeratin 10, involucrin, and loricrin) was confirmed by immunohistochemistry and Western blot analysis. The nylon reconstructed human epidermis model’s applicability was evaluated as a platform to perform irritation and corrosion tests. Our results demonstrated that this model is a promising platform to assess the potential of dermal irritation/corrosion of chemical products.

scholarly journals Knockdown of Filaggrin in a Three-Dimensional Reconstructed Human Epidermis Impairs Keratinocyte Differentiation

2014 ◽  
Vol 134 (12) ◽  
pp. 2938-2946 ◽  
Author(s):  
Valérie Pendaries ◽  
Jeremy Malaisse ◽  
Laurence Pellerin ◽  
Marina Le Lamer ◽  
Rachida Nachat ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Human Epidermis ◽  
Keratinocyte Differentiation ◽  
Reconstructed Human Epidermis

Implementation, availability and regulatory status of an OECD accepted Reconstructed Human Epidermis model in Brazil

2018 ◽  
Vol 6 (1) ◽  
pp. 64 ◽  
Author(s):  
Rodrigo De Vecchi ◽  
Vanja Dakic ◽  
Guilherme Mattos ◽  
Anne-Sophie Rigaudeau ◽  
Veronica Oliveira ◽  
...  
Keyword(s):  
Human Epidermis ◽  
Reconstructed Human Epidermis ◽  
Regulatory Status

scholarly journals A Review on Additive Manufacturing Possibilities for Electrical Machines

Energies ◽  
2021 ◽  
Vol 14 (7) ◽  
pp. 1940
Author(s):  
Muhammad Usman Naseer ◽  
Ants Kallaste ◽  
Bilal Asad ◽  
Toomas Vaimann ◽  
Anton Rassõlkin
Keyword(s):  
Additive Manufacturing ◽  
Large Scale ◽  
Three Dimensional ◽  
Electrical Machines ◽  
Electromagnetic Properties ◽  
Scale Production ◽  
Performance Parameters ◽  
Large Scale Production ◽  
One Step ◽  
Manufacturing Techniques

This paper presents current research trends and prospects of utilizing additive manufacturing (AM) techniques to manufacture electrical machines. Modern-day machine applications require extraordinary performance parameters such as high power-density, integrated functionalities, improved thermal, mechanical & electromagnetic properties. AM offers a higher degree of design flexibility to achieve these performance parameters, which is impossible to realize through conventional manufacturing techniques. AM has a lot to offer in every aspect of machine fabrication, such that from size/weight reduction to the realization of complex geometric designs. However, some practical limitations of existing AM techniques restrict their utilization in large scale production industry. The introduction of three-dimensional asymmetry in machine design is an aspect that can be exploited most with the prevalent level of research in AM. In order to take one step further towards the enablement of large-scale production of AM-built electrical machines, this paper also discusses some machine types which can best utilize existing developments in the field of AM.

scholarly journals Vibration characteristics of triple-gear-rotor system in compressed air energy storage under variable torque load

2021 ◽  
Vol 104 (1) ◽  
pp. 003685042098705
Author(s):  
Xinran Wang ◽  
Yangli Zhu ◽  
Wen Li ◽  
Dongxu Hu ◽  
Xuehui Zhang ◽  
...  
Keyword(s):  
Large Scale ◽  
Three Dimensional ◽  
Element Model ◽  
Rotor System ◽  
Dynamic Responses ◽  
System Response ◽  
Rotating Speed ◽  
Torque Load ◽  
Set Up ◽  
Two Stages

This paper focuses on the effects of the off-design operation of CAES on the dynamic characteristics of the triple-gear-rotor system. A finite element model of the system is set up with unbalanced excitations, torque load excitations, and backlash which lead to variations of tooth contact status. An experiment is carried out to verify the accuracy of the mathematical model. The results show that when the system is subjected to large-scale torque load lifting at a high rotating speed, it has two stages of relatively strong periodicity when the torque load is light, and of chaotic when the torque load is heavy, with the transition between the two states being relatively quick and violent. The analysis of the three-dimensional acceleration spectrum and the meshing force shows that the variation in the meshing state and the fluctuation of the meshing force is the basic reasons for the variation in the system response with the torque load. In addition, the three rotors in the triple-gear-rotor system studied show a strong similarity in the meshing states and meshing force fluctuations, which result in the similarity in the dynamic responses of the three rotors.

Sign in / Sign up

Export Citation Format

Share Document