scholarly journals Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx

10.3791/62154 ◽  
2021 ◽  
Author(s):  
Ruichen Sun ◽  
Daniel Brogan ◽  
Anna Buchman ◽  
Ting Yang ◽  
Omar S. Akbari
Keyword(s):  
Drosophila Melanogaster ◽  
Tissue Specific ◽  
Rna Targeting

scholarly journals Sex and tissue‐specific evolution of developmental plasticity in Drosophila melanogaster

2020 ◽  
Author(s):  
Didem P. Sarikaya ◽  
Katherine Rickelton ◽  
Julie M. Cridland ◽  
Ryan Hatmaker ◽  
Hayley K. Sheehy ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Developmental Plasticity ◽  
Tissue Specific

scholarly journals Three-dimensional organization of Drosophila melanogaster interphase nuclei. I. Tissue-specific aspects of polytene nuclear architecture.

1987 ◽  
Vol 104 (6) ◽  
pp. 1455-1470 ◽  
Author(s):  
M Hochstrasser ◽  
J W Sedat
Keyword(s):  
Drosophila Melanogaster ◽  
Three Dimensional ◽  
Nuclear Architecture ◽  
Close Packing ◽  
Prothoracic Gland ◽  
Cell Type ◽  
Interphase Chromosome ◽  
Tissue Specific ◽  
Spatial Domains ◽  
Four Levels

Interphase chromosome organization in four different Drosophila melanogaster tissues, covering three to four levels of polyteny, has been analyzed. The results are based primarily on three-dimensional reconstructions from unfixed tissues using a computer-based data collection and modeling system. A characteristic organization of chromosomes in each cell type is observed, independent of polyteny, with some packing motifs common to several or all tissues and others tissue-specific. All chromosomes display a right-handed coiling chirality, despite large differences in size and degree of coiling. Conversely, in each cell type, the heterochromatic centromeric regions have a unique structure, tendency to associate, and intranuclear location. The organization of condensed nucleolar chromatin is also tissue-specific. The tightly coiled prothoracic gland chromosomes are arrayed in a similar fashion to the much larger salivary gland chromosomes described previously, having polarized orientations, nonintertwined spatial domains, and close packing of the arms of each autosome, whereas hindgut and especially the unusually straight midgut chromosomes display striking departures from these regularities. Surprisingly, gut chromosomes often appear to be broken in the centric heterochromatin. Severe deformations of midgut nuclei observed during gut contractions in living larvae may account for their unusual properties. Finally, morphometric measurements of chromosome and nuclear dimensions provide insights into chromosome growth and substructure and also suggest an unexpected parallel with diploid chromatin organization.

scholarly journals Highly Tissue Specific Expression of Sphinx Supports Its Male Courtship Related Role in Drosophila melanogaster

PLoS ONE ◽  
2011 ◽  
Vol 6 (4) ◽  
pp. e18853 ◽  
Author(s):  
Ying Chen ◽  
Hongzheng Dai ◽  
Sidi Chen ◽  
Luoying Zhang ◽  
Manyuan Long
Keyword(s):  
Drosophila Melanogaster ◽  
Male Courtship ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression

A novel, tissue-specific integrin subunit, beta nu, expressed in the midgut of Drosophila melanogaster

Development ◽  
1993 ◽  
Vol 118 (3) ◽  
pp. 845-858 ◽  
Author(s):  
G.H. Yee ◽  
R.O. Hynes
Keyword(s):  
Drosophila Melanogaster ◽  
Matrix Proteins ◽  
Beta Subunits ◽  
Integrin Subunit ◽  
Integrin Receptor ◽  
Tissue Specific ◽  
Surface Receptors ◽  
Polymerase Chain ◽  
Maximal Expression

The integrins are a family of cell surface receptors for extracellular matrix proteins and counter-receptors on other cells. We have used the polymerase chain reaction to identify a novel integrin receptor beta subunit in Drosophila melanogaster. The deduced amino acid sequence of this subunit, which we have termed beta v (beta-neu), indicates that it has several unusual properties. The beta v subunit is roughly 33% identical with each of the previously sequenced vertebrate and Drosophila beta subunits and is lacking four of the 56 cysteine residues characteristic of most members of this protein family. The expression of the beta v gene is strikingly restricted. It is temporally regulated, with maximal expression occurring at 12–15 hours of embryonic development. In situ hybridization analyses and antibody localization on whole-mount embryos reveal that beta v expression is tissue-specific and confined to the developing midgut endoderm and its precursors during embryogenesis. Tissue specificity of expression is maintained through later stages of development as beta v transcripts are found exclusively in the larval midgut. Within this structure, beta v transcripts are especially concentrated in the cells of the midgut imaginal islands which give rise to the adult midgut.

Tissue-specific transcription of the neuronal gene Lim3 affects Drosophila melanogaster lifespan and locomotion

2017 ◽  
Vol 18 (5) ◽  
pp. 739-757 ◽  
Author(s):  
Olga Y. Rybina ◽  
Svetlana V. Sarantseva ◽  
Ekaterina R. Veselkina ◽  
Olga I. Bolschakova ◽  
Alexander V. Symonenko ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Tissue Specific ◽  
Neuronal Gene

scholarly journals Tissue-specific expression of the diazepam-binding inhibitor in Drosophila melanogaster: cloning, structure, and localization of the gene.

1994 ◽  
Vol 14 (10) ◽  
pp. 6983-6995 ◽  
Author(s):  
M Kolmer ◽  
C Roos ◽  
M Tirronen ◽  
S Myöhänen ◽  
H Alho
Keyword(s):  
Drosophila Melanogaster ◽  
Transcription Initiation ◽  
Coenzyme A ◽  
Cpg Island ◽  
High Energy ◽  
Malpighian Tubules ◽  
Rnase Protection ◽  
Tissue Specific ◽  
Receptor Modulation ◽  
Acyl Coenzyme A

The diazepam-binding inhibitor (DBI; also called acyl coenzyme A-binding protein or endozepine) is a 10-kDa polypeptide found in organisms ranging from yeasts to mammals. It has been shown that DBI and its processing products are involved in various specific biological processes such as GABAA/benzodiazepine receptor modulation, acyl coenzyme A metabolism, steroidogenesis, and insulin secretion. We have cloned and sequenced the Drosophila melanogaster gene and cDNA encoding DBI. The Drosophila DBI gene encodes a protein of 86 amino acids that shows 51 to 56% identity with previously known DBI proteins. The gene is composed of one noncoding 5' and two coding exons and is localized on the chromosomal map at position 65E. Several transcription initiation sites were detected by RNase protection and primer extension experiments. Computer analysis of the promoter region revealed features typical of housekeeping genes, such as the lack of TATA and CCAAT elements. However, in its low GC content and lack of a CpG island, the region resembles promoters of tissue-specific genes. Northern (RNA) analysis revealed that the expression of the DBI gene occurred from the larval stage onwards throughout the adult stage. In adult flies, DBI mRNA and immunoreactivity were detected in the cardia, part of the Malpighian tubules, the fat body, and gametes of both sexes. Developmentally regulated expression, disappearing during metamorphosis, was detected in the larval and pupal brains. No expression was detected in the adult nervous system. On the basis of the expression of DBI in some but not all tissues with high energy consumption, we propose that in D. melanogaster, DBI is involved in energy metabolism in a manner that depends on the substrate used for energy production.

scholarly journals Smart functional nucleic acid chimeras: Enabling tissue specific RNA targeting therapy

RNA Biology ◽  
2015 ◽  
Vol 12 (4) ◽  
pp. 412-425 ◽  
Author(s):  
Lukas J Aaldering ◽  
Hossam Tayeb ◽  
Shilpa Krishnan ◽  
Susan Fletcher ◽  
Stephen D Wilton ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Tissue Specific ◽  
Targeting Therapy ◽  
Rna Targeting
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