Molecular Spring Constant Analysis by Biomembrane Force Probe Spectroscopy

10.3791/62490 ◽  
2021 ◽  
Author(s):  
Peyman Obeidy ◽  
Haoqing Wang ◽  
Mingqin Du ◽  
Huiqian Hu ◽  
Fang Zhou ◽  
...  
Keyword(s):  
Spring Constant ◽  
Biomembrane Force Probe ◽  
Probe Spectroscopy

scholarly journals Benchmarks of Biomembrane Force Probe Spring Constant Models

2017 ◽  
Vol 113 (12) ◽  
pp. 2842-2845 ◽  
Author(s):  
Lining Ju ◽  
Cheng Zhu
Keyword(s):  
Spring Constant ◽  
Biomembrane Force Probe

ENERGY-RESOLVED ATOM PROBE SPECTROSCOPY OF FIELD-ADSORPTION PHENOMENA

1988 ◽  
Vol 49 (C6) ◽  
pp. C6-197-C6-202
Author(s):  
N. ERNST ◽  
G. BOZDECH
Keyword(s):  
Atom Probe ◽  
Probe Spectroscopy ◽  
Adsorption Phenomena

Limits of Charge Carrier Transport in Halide Perovskites Revealed by Optical-Pump Terahertz-Probe Spectroscopy

2019 ◽  
Author(s):  
Hannes Hempel ◽  
Andrei Petsiu ◽  
Martin Stolterfoht ◽  
Pascal Becker ◽  
Dieter Neher ◽  
...  
Keyword(s):  
Charge Carrier ◽  
Carrier Transport ◽  
Charge Carrier Transport ◽  
Optical Pump ◽  
Halide Perovskites ◽  
Probe Spectroscopy

scholarly journals Biomechanics of Neutrophil Tethers

Life ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 515
Author(s):  
Andrea Cugno ◽  
Alex Marki ◽  
Klaus Ley
Keyword(s):  
Cell Activation ◽  
Optical Trap ◽  
Biomechanical Properties ◽  
Force Microscopy ◽  
Advantages And Disadvantages ◽  
Atomic Force ◽  
Microfluidic Flow ◽  
Biomembrane Force Probe ◽  
Membrane Reservoir

Leukocytes, including neutrophils, which are propelled by blood flow, can roll on inflamed endothelium using transient bonds between selectins and their ligands, and integrins and their ligands. When such receptor–ligand bonds last long enough, the leukocyte microvilli become extended and eventually form thin, 20 m long tethers. Tether formation can be observed in blood vessels in vivo and in microfluidic flow chambers. Tethers can also be extracted using micropipette aspiration, biomembrane force probe, optical trap, or atomic force microscopy approaches. Here, we review the biomechanical properties of leukocyte tethers as gleaned from such measurements and discuss the advantages and disadvantages of each approach. We also review and discuss viscoelastic models that describe the dependence of tether formation on time, force, rate of loading, and cell activation. We close by emphasizing the need to combine experimental observations with quantitative models and computer simulations to understand how tether formation is affected by membrane tension, membrane reservoir, and interactions of the membrane with the cytoskeleton.

scholarly journals Ultrafast Electron/Energy Transfer and Intersystem Crossing Mechanisms in BODIPY-Porphyrin Compounds

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 312
Author(s):  
Yusuf Tutel ◽  
Gökhan Sevinç ◽  
Betül Küçüköz ◽  
Elif Akhuseyin Yildiz ◽  
Ahmet Karatay ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Spectra ◽  
Visible Region ◽  
Energy Transfer Mechanism ◽  
Free Base ◽  
Pump Probe ◽  
Harvesting Efficiency ◽  
Probe Spectroscopy ◽  
Absorption Features ◽  
Transfer Mechanisms

Meso-substituted borondipyrromethene (BODIPY)-porphyrin compounds that include free base porphyrin with two different numbers of BODIPY groups (BDP-TTP and 3BDP-TTP) were designed and synthesized to analyze intramolecular energy transfer mechanisms of meso-substituted BODIPY-porphyrin dyads and the effect of the different numbers of BODIPY groups connected to free-base porphyrin on the energy transfer mechanism. Absorption spectra of BODIPY-porphyrin conjugates showed wide absorption features in the visible region, and that is highly valuable to increase light-harvesting efficiency. Fluorescence spectra of the studied compounds proved that BODIPY emission intensity decreased upon the photoexcitation of the BODIPY core, due to the energy transfer from BODIPY unit to porphyrin. In addition, ultrafast pump-probe spectroscopy measurements indicated that the energy transfer of the 3BDP-TTP compound (about 3 ps) is faster than the BDP-TTP compound (about 22 ps). Since the BODIPY core directly binds to the porphyrin unit, rapid energy transfer was seen for both compounds. Thus, the energy transfer rate increased with an increasing number of BODIPY moiety connected to free-base porphyrin.

Dirac Surface Plasmons in Photoexcited Bismuth Telluride Nanowires : Optical Pump-Terahertz Probe Spectroscopy

Nanoscale ◽  
2021 ◽  
Author(s):  
Mithun K P ◽  
Srabani Kar ◽  
Abinash Kumar ◽  
Victor Suvisesha Muthu Dharmaraj ◽  
Ravishankar Narayanan ◽  
...  
Keyword(s):  
Surface Plasmons ◽  
Bismuth Telluride ◽  
Topological Insulators ◽  
Collective Excitation ◽  
Optical Pump ◽  
Time Resolved ◽  
Mid Infrared ◽  
Plasmonic Materials ◽  
Probe Spectroscopy

Collective excitation of Dirac plasmons in graphene and topological insulators have opened new possibilities of tunable plasmonic materials ranging from THz to mid-infrared regions. Using time resolved Optical Pump -...

scholarly journals A Flexible PI/Si/SiO2 Piezoresistive Microcantilever for Trace-Level Detection of Aflatoxin B1

Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1118
Author(s):  
Yuan Tian ◽  
Yi Liu ◽  
Yang Wang ◽  
Jia Xu ◽  
Xiaomei Yu
Keyword(s):  
Single Crystal ◽  
Aflatoxin B1 ◽  
Single Crystal Silicon ◽  
Silicon Layer ◽  
Wheatstone Bridge ◽  
Silicon On Insulator ◽  
Passivation Layer ◽  
Spring Constant ◽  
Trace Level ◽  
Crystal Silicon

In this paper, a polyimide (PI)/Si/SiO2-based piezoresistive microcantilever biosensor was developed to achieve a trace level detection for aflatoxin B1. To take advantage of both the high piezoresistance coefficient of single-crystal silicon and the small spring constant of PI, the flexible piezoresistive microcantilever was designed using the buried oxide (BOX) layer of a silicon-on-insulator (SOI) wafer as a bottom passivation layer, the topmost single-crystal silicon layer as a piezoresistor layer, and a thin PI film as a top passivation layer. To obtain higher sensitivity and output voltage stability, four identical piezoresistors, two of which were located in the substrate and two integrated in the microcantilevers, were composed of a quarter-bridge configuration wheatstone bridge. The fabricated PI/Si/SiO2 microcantilever showed good mechanical properties with a spring constant of 21.31 nN/μm and a deflection sensitivity of 3.54 × 10−7 nm−1. The microcantilever biosensor also showed a stable voltage output in the Phosphate Buffered Saline (PBS) buffer with a fluctuation less than 1 μV @ 3 V. By functionalizing anti-aflatoxin B1 on the sensing piezoresistive microcantilever with a biotin avidin system (BAS), a linear aflatoxin B1 detection concentration resulting from 1 ng/mL to 100 ng/mL was obtained, and the toxic molecule detection also showed good specificity. The experimental results indicate that the PI/Si/SiO2 flexible piezoresistive microcantilever biosensor has excellent abilities in trace-level and specific detections of aflatoxin B1 and other biomolecules.

Optical pump-terahertz probe spectroscopy utilizing a cavity-dumped oscillator-driven terahertz spectrometer

1998 ◽  
Vol 4 (2) ◽  
pp. 353-359 ◽  
Author(s):  
B.N. Flanders ◽  
D.C. Arnett ◽  
N.F. Scherer
Keyword(s):  
Optical Pump ◽  
Probe Spectroscopy

Photocarriers dynamics in silicon wafer studied with optical-pump terahertz-probe spectroscopy

2012 ◽  
Vol 285 (20) ◽  
pp. 4102-4106 ◽  
Author(s):  
Gaofang Li ◽  
Dong Li ◽  
Zuanming Jin ◽  
Guohong Ma
Keyword(s):  
Silicon Wafer ◽  
Optical Pump ◽  
Probe Spectroscopy
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