Isolation and Genotyping Study of Clostridium Perfringens From Broiler Farms Infected with Necrotic Enteritis in Sulaimania Province

The study is conducting to isolate and toxinotype the suspected cases of Clostridium perfringens infections of broiler farms in Sulaimania province. A total of 108 samples were collected from intestinal contents, mucosal scraping, and hemorrhagic lymphoid nodules from suspected cases of necrotic enteritis in broilers. The result of isolated and identified bacteria were revealed that 63 (58%) out of 108 samples were positive for C. perfringens. The results revealed that the isolates were only positive for alpha and beta2 toxin genes. Phylogenetic and DNA sequence analysis of cpa and cpb2 gene showed that cpa genes were highly identical to isolates from broiler in Iran, poultry stool and broiler in Brazil, and blue calves in Belgium. While cpb2 gene is closely related to the isolates of broiler in Iran, India and isolates of goat in Pakistan. The results indicated that the causative agent of necrotic enteritis in broiler farms in the region was mainly due to C. perfringens type A infection

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Haemorrhagic-necrotic enteritis in heavy breeds broilers

Veterinarski glasnik ◽

10.2298/vetgl1404265j ◽

2014 ◽

Vol 68 (3-4) ◽

pp. 265-273

Author(s):

Nemanja Jezdimirovic ◽

Branislav Kureljusic ◽

Vojin Ivetic ◽

Jasna Kureljusic ◽

Dj. Cvetojevic ◽

...

Keyword(s):

Small Intestine ◽

Clostridium Perfringens ◽

Histopathological Examination ◽

Type A ◽

Necrotic Enteritis ◽

Bacteriological Examination ◽

Liquid Content ◽

Anaerobic Conditions ◽

Intestinal Contents

The aim of the investigation was to determine the influence of Clostridium perfringens type A on the development of pathomorphological substrate, its intensity and distribution in fifteen weeks old heavy breeds broilers. The investigation was carried out on corpses of 8 hens and 7 roosters of heavy breeds of provenance COBB 500. After the completion of the autopsy, samples of altered parts of jejunum and liver were taken for histopathological examination, and jejunum intestinal contents for bacteriological examination. In all the corpses, in open pleuroperitoneal cavity, even in situ, an altered part of jejunum can be noticed. It was extremely dilated the entire length, and its wall was bluish-gray with disseminated subserous punctiform blood extravasates. When opened, semi-liquid content with blood coagulums and patches of necrotic mucosa went out of it. By microscopic examination of small intestine tissue cuttings, colored by HE method, there was observed a diffuse necrosis of intestinal villi. They were desroyed and replaced by eosinophilic structureless mass. Furthermore, there could be noticed submucose oedema, capillary congestion and blood extravasates in mucosa, as well as infiltration of neutrophilic granulocytes in lamina propria. These microscopic alterations reflect hemorrhagic necrotic enteritis. By microscopic examining of small intestine cuttings colored according to Brown & Brenn method, colonies of bacteria in distal parts of the submucosa were found out. Using bacteriological tests in anaerobic conditions, there was isolated a culture identified as Clostridium perfringens. After applying of multiplex PCR, the obtained isolate was genotyped as Clostridium perfringens type A. On the basis of pathomorphological, bacteriological and molecular examinations, it can be concluded that the infection of heavy breeds with Clostridium perfringens type A is manifested by appearance of haemorrhagic-necrotic jejunitis, that the causer penetrates deeply into jejunum tissue and that wheat and wheat bran were a favoring factor for proliferation of the etiological agent.

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Isolation, identification and toxinotyping of Clostridium perfringens isolated from broilers in Pakistan

The Pakistan Journal of Agricultural Sciences ◽

10.21162/pakjas/21.1414 ◽

2021 ◽

Vol 58 (04) ◽

pp. 1367-1372

Author(s):

Zain Ul Abadeen

Keyword(s):

Clostridium Perfringens ◽

Causative Agent ◽

Broiler Chicken ◽

Clinical Signs ◽

Type A ◽

Toxin Gene ◽

Necrotic Enteritis ◽

Alpha Toxin ◽

Enteric Disease ◽

Isolation Rate

Necrotic enteritis (NE) is one of the important enteric disease in the poultry industry worldwide, caused by C. perfringens type A. This study describes the isolation, identification, and toxinotyping of C. perfringens in necrotic enteritis affected broiler chicken in Pakistan. A total of 430 intestinal samples from dead carcasses and birds suspected of NE outbreak, in and around Faisalabad, Pakistan were collected from 36 broiler farms which yielded 87 alpha toxin gene (cpa) positive C. perfringens type A isolates. The birds having 4-5 weeks of age, clinical signs, and reared in open (conventional) sheds showed higher C. perfringens isolation rate. The study concluded netB negative C. perfringens type A as a causative agent for NE outbreaks in broiler birds in Faisalabad, Pakistan.

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Spontaneous necrotic enteritis in young RFM/Ms mice

Laboratory Animals ◽

10.1258/002367786780865124 ◽

1986 ◽

Vol 20 (2) ◽

pp. 114-117 ◽

Cited By ~ 6

Author(s):

S. Matsushita ◽

T. Matsumoto

Keyword(s):

Drinking Water ◽

Clostridium Perfringens ◽

Inflammatory Cell ◽

Type A ◽

Necrotic Enteritis ◽

Cell Reaction ◽

Breeding Colony ◽

Conventional Condition ◽

Intestinal Contents ◽

Gross Lesions

Fatal necrotic enteritis was observed in mice 24-52 days old in the RFM/Ms breeding colony maintained in a clean conventional condition in the National Institute of Radiological Sciences. Gross lesions included hyperaemia, petechiae, erosion and the occasional formation of pseudomembranes in the mucosa of the ileum and caecum. Histologically, there was necrotic enteritis with numerous Gram-positive bacilli-forming spores but no inflammatory cell reaction. Non-type-A Clostridium perfringens was isolated from the intestinal contents. This disease cleared after the addition of chlortetracycline hydrochloride (11 mg/I) to the drinking water.

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