scholarly journals ARBUSCULAR MYCORRHIZAL FUNGI ASSOCIATED WITH PEPPER TEN LINES RHIZOSPHERE CHILLIES Capsicum frutescens

2019 ◽  
Vol 3 (1) ◽  
pp. 107-115
Author(s):  
Wagner Gonçalves Vieira Junior ◽  
Diogo Jânio de Carvalho Matos ◽  
Thales Caetano de Oliveira ◽  
Leidiane Dos Santos Lucas ◽  
Isabelly Ribeiro Lima ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Culture Collection ◽  
Mycorrhizal Colonization ◽  
Colonization Rate ◽  
Spore Density ◽  
Capsicum Frutescens ◽  
Morphological Comparison ◽  
Significant Difference

The peppers business is an important segment of the Brazilian agricultural market, with strong expression in the food, pharmaceutical and cosmetic industries. The objective of this work was to determine the mycorrhizal colonization rate, spore density and mycorrhizal fungi genotypes associated with the rhizosphere of ten lines of chilli peppers (Capsicum frutescens). The experimental design was completely randomized, with 10 treatments and 4 replicates, with the ten strains of Capsicum frutescens: IFET-1121; IFET-1109; IFET-1129; IFET-1119; IFET-1117; IFET-1137; IFET-1131; IFET-1127; IFET-1125 and IFET-1111. The spores of arbuscular mycorrhizal fungi (AMF) will be extracted from the soil using 50 cm³ of each composite sample, using the wet sieving technique. The determination of the percentage of mycorrhizal colonization occurred through the technique of intersection of the quadrants. The identification of mycorrhizal fungi species was by morphological comparison based on the descriptions of the reference cultures present in the International Culture Collection of Arbuscular and Vesicular-Arbuscular Mycorrhizal Fungi. The spore number and mycorrhizal colonization data will be submitted to classical statistical analysis using the Assistat program (2016). The genus Acaulospora, Claroideoglomus, Diversispora, Scutellospora, Sclerocystis, Glomus, Funneliformis and Gigaspora associated with the rhizosphere of the Capsicum frutescens strains were identified. The genera Glomus, acaulosporand Claroideoglomuswere found in all strains analyzed. The IFET - 1127 strain presented higher spore density values when compared to the other strains studied. No significant difference was found in the values of mycorrhizal colonization rate among the investigated strains

The influence of compost and arbuscular mycorrhizal fungi on sugarcane growth and nutrient uptake

BioResources ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. 3005-3016
Author(s):  
Gustavo Mattos Abreu ◽  
Gabrielly dos Santos Bobadilha ◽  
Bruna Duque Guirardi ◽  
Phillipe Mattos Abreu ◽  
Naelmo de Souza Oliviera ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Nutrient Uptake ◽  
Biomass Production ◽  
Mycorrhizal Fungi ◽  
Organic Matter Content ◽  
Arbuscular Mycorrhizal ◽  
Mycorrhizal Colonization ◽  
Colonization Rate ◽  
Initial Growth ◽  
Mycorrhizal Dependency

Compost and Arbuscular mycorrhizal fungi are environmentally sustainable and low-cost materials that can benefit tropical soils with high phosphorus fixation and low organic matter content. This study investigated the effects of Arbuscular mycorrhizal fungi (AMF) and compost on the growth and nutrient uptake efficiency of sugarcane (Saccharum officinarum) seedlings. The experimental design was a completely randomized factorial design, where factor A (n = 5) was the compost doses (0, 15, 30, 60 and 120 t ha-1) and B (n = 3) the AMF inoculum (Rhizophagus clarus, Gigaspora margarita, and non-inoculated). At 30 and 90 d, seedlings’ diameter and height were measured. Mycorrhizal colonization rate, biomass production, nutrient uptake (P and N), and mycorrhizal dependency were assessed at the end of the experiment. The AMF and compost doses affected the colonization rate, initial growth, biomass production, and nutrient uptake of sugarcane seedlings. Overall, the AMF benefited plant growth at lower doses of compost. R. clarus had a higher impact on the shoot diameter of sugarcane seedlings. Mycorrhizal colonization increased with compost addition only in seedlings inoculated with G. margarita. There was no clear trend among AMF treatments for nutrient uptake. In general, sugarcane seedlings dependency on mycorrhizal condition to produce growth was higher at lower compost doses.

scholarly journals Influence of Land Use Intensity and Management on Arbuscular Mycorrhizal Fungi-Avocado Symbiosis

2021 ◽  
Vol 13 (3) ◽  
pp. 10
Author(s):  
P. G. Soti ◽  
Bulent Toprak ◽  
Nina De La Rosa ◽  
Krish Jayachandran
Keyword(s):  
Land Use ◽  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Management Practices ◽  
Arbuscular Mycorrhizal ◽  
Soil Characteristics ◽  
Mycorrhizal Colonization ◽  
Persea Americana ◽  
South Florida ◽  
Significant Difference

This study was done to assess the effect of soil disturbance on arbuscular mycorrhizal fungi spore abundance and root colonization in avocado (Persea americana Mill.). Rhizosphere soil and root samples of avocado were collected from different farms in south Florida and analyzed for degree of mycorrhizal colonization in roots, spore density and diversity in soil along with soil characteristics. There was significant difference in the soil characteristics among the different farms. Similarly, there was a significant difference in the degree of mycorrhizal colonization in the roots and the arbuscular mycorrhizal fungi spore morphotypes among different land use and management practices. However, there was no significant difference in the total number of arbuscular mycorrhizal fungi spores among these sites. There was no correlation between the number of arbuscular mycorrhizal fungi spores and soil characteristics. However, arbuscular mycorrhizal fungi colonization in roots were strongly influenced by soil characteristics such as soil moisture, carbon, nitrogen, and organic matter. Further research is necessary to identify these AMF species and determine the role of in avocado growth tolerance to anthropogenic disturbance in highly disturbed urban soils.

scholarly journals FUNGOS MICORRÍZICOS ARBUSCULARES EM PLANTIOS DE DENDÊ (Elaeis guineensis Jacq.):

2020 ◽  
Vol 4 (2) ◽  
pp. e042005
Author(s):  
Carla Silva Sousa ◽  
Francisco de Sousa Lima ◽  
Silvane de Souza De Jesus
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Oil Palm ◽  
Mycorrhizal Fungi ◽  
Elaeis Guineensis ◽  
Arbuscular Mycorrhizal ◽  
Mycorrhizal Colonization ◽  
Biodiesel Production ◽  
Spore Density ◽  
Production Chain ◽  
Technological Level

O Brasil ocupa posição de destaque na produção e geração de energia renovável de origem agrícola e o dendezeiro está entre as oleaginosas que apresentam maior potencial para produção de biodiesel. A dendeicultura encontrou na região Baixo Sul da Bahia condições edafoclimáticas ideais para seu cultivo, apesar de enfrentar alguns entraves, sobretudo no que diz respeito ao baixo nível tecnológico da cadeia produtiva e a presença predominante de plantios antigos que ultrapassam o período de potencial produção da cultura. A investigação da ocorrência de fungos micorrízicas arbusculares constitui um dos componentes ecológicos importantes em plantios e ecossistemas quando se deseja obter o máximo de associação das espécies micorrízicas em benefício de espécies vegetais. Assim, com o objetivo de avaliar a ocorrência de fungos micorrízicos arbusculares foram coletadas amostras de solo e raízes em plantas de dendê das variedades Dura e Tenera, em três diferentes áreas de plantios de dendezeiros definidas como AR-1(área 1), AR-2 (área 2) e AR-3 (área 3), localizados no município de Valença-BA. Foi realizada a determinação da densidade de esporos nas amostras de solo e a taxa de colonização micorrízica nas amostras de raízes. A partir dos resultados obtidos, constatou-se maior densidade de esporos no solo rizosférico de plantas da variedade Tenera (valores entre 91,2 e 446,6 esporos/30g de solo) em comparação às plantas da variedade Dura (valores entre 41,8 e 292,4 esporos/30 g de solo). Plantas da variedade Tenera também apresentaram maior taxa de colonização micorrízica quando comparadas às plantas da variedade Dura. Independente da variedade estudada, na AR-3 foi registrada maior densidade de esporos e colonização micorrízica, em relação às demais áreas. Abstract The production and generation of renewable energy of agricultural origin plays an important role in Brazil. Among the oilseeds, oil palm has great potential for biodiesel production. Despite facing obstacles regarding the low technological level of the production chain and the predominant presence of old plantations, oil palm has found ideal edaphoclimatic conditions in Bahia’s Baixo Sul region. Among several beneficial microorganisms associated with plants, arbuscular mycorrhizal fungi stand out for promoting improvements in plant nutrition and plant health, making them resistant to adverse environmental factors. Thus, in order to assess the occurrence of arbuscular mycorrhizal fungi, soil and roots samples were collected from oil palm plants of Dura and Tenera varieties, in three different oil palm field areas defined as: AR-1 (area 1), AR- 2 (area 2) and AR-3 (area 3), located in the municipality of Valença, Bahia. The spore density in soil samples and the rate of mycorrhizal colonization in root samples were determined. A higher density of spores was found in the rhizospheric soil of the variety Tenera (values ranging from 91.2 to 446.6 spores / 30 g of soil) compared to plants of the variety Dura (values ranging from 41.8 to 292.4 spores / 30 g of soil). Oil palm plants of Tenera variety also showed a higher rate of mycorrhizal colonization when compared to plants of Dura variety. Regardless of the variety, AR-3 revealed a higher density of spores and mycorrhizal colonization compared to the other areas. Keywords: Mycorrhizal colonization, Oil palm, Spontaneous planting.

scholarly journals Response of Arbuscular Mycorrhizal Fungi to Simulated Climate Changes by Reciprocal Translocation in Tibetan Plateau

2015 ◽  
Vol 43 (2) ◽  
pp. 488-493
Author(s):  
Zhaoyong SHI ◽  
Xubin YIN ◽  
Bede MICKAN ◽  
Fayuan WANG ◽  
Ying ZHANG ◽  
...  
Keyword(s):  
Tibetan Plateau ◽  
Arbuscular Mycorrhizal Fungi ◽  
Reciprocal Translocation ◽  
Mycorrhizal Fungi ◽  
Climate Changes ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Spore Density ◽  
The Tibetan Plateau ◽  
Colonization Frequency

Arbuscular mycorrhiza (AM) fungi are considered as an important factor in predicting plants and ecosystem responses to climate changes on a global scale. The Tibetan Plateau is the highest region on Earth with abundant natural resources and one of the most sensitive region to climate changes. To evaluate the complex response of arbuscular mycorrhizal fungi colonization and spore density to climate changes, a reciprocal translocation experiment was employed in Tibetan Plateau. The reciprocal translocation of quadrats to AM colonization and spore density were dynamic. Mycorrhizal colonization frequency presented contrary changed trend with elevations of quadrat translocation. Colonization frequency reduced or increased in majority quadrats translocated from low to high or from high to low elevation. Responses of colonization intensity to translocation of quadrats were more sensitive than colonization frequency. Arbuscular colonization showed inconsistent trend in increased or decreased quadrat. Vesicle colonization decreased with changed of quadrat from low to high elevations. However, no significant trend was observed. Although spore density was dynamic with signs of decreasing or increasing in translocated quadrats, the majority enhanced and declined respectively in descent and ascent quadrat treatments. It is crucial to understand the interactions between AM fungi and prairie grasses to accurately predict effects of climate change on these diverse and sensitive ecosystems. This study provided an opportunity for understanding the effect of climate changes on AM fungi.

scholarly journals Community Analysis of Arbuscular Mycorrhizal Fungi in Roots ofPoncirus trifoliataandCitrus reticulataBased on SSU rDNA

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Peng Wang ◽  
Yin Wang
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Glomus Intraradices ◽  
Arbuscular Mycorrhizal ◽  
Small Subunit ◽  
Poncirus Trifoliata ◽  
Spore Density ◽  
Morphological Observation ◽  
Trifoliate Orange ◽  
Hyphal Length

Morphological observation of arbuscular mycorrhizal fungi (AMF) species in rhizospheric soil could not accurately reflect the actual AMF colonizing status in roots, while molecular identification of indigenous AMF colonizing citrus rootstocks at present was rare in China. In our study, community of AMF colonizing trifoliate orange (Poncirus trifoliataL. Raf.) and red tangerine (Citrus reticulataBlanco) were analyzed based on small subunit of ribosomal DNA genes. Morphological observation showed that arbuscular mycorrhizal (AM) colonization, spore density, and hyphal length did not differ significantly between two rootstocks. Phylogenetic analysis showed that 173 screened AMF sequences clustered in at least 10 discrete groups (GLO1~GLO10), all belonging to the genus ofGlomusSensu Lato. Among them, GLO1 clade (clustering with uncultured Glomus) accounting for 54.43% clones was the most common in trifoliate orange roots, while GLO6 clade (clustering withGlomus intraradices) accounting for 35.00% clones was the most common in red tangerine roots. Although, Shannon-Wiener indices exhibited no notable differences between both rootstocks, relative proportions of observed clades analysis revealed that composition of AMF communities colonizing two rootstocks varied severely. The results indicated that native AMF species in citrus rhizosphere had diverse colonization potential between two different rootstocks in the present orchards.

scholarly journals Isolation and identification of indigenous Arbuscular Mycorrhizal Fungi (AMF) of forest clove rhizosphere from Maluku, Indonesia

2021 ◽  
Vol 22 (8) ◽  
Author(s):  
Asri Subkhan Mahulette ◽  
Anggra Alfian ◽  
ABDUL KARIM KILKODA ◽  
IMELDA JEANETTE LAWALATA ◽  
DESSY ARIYANI MARASABESSY ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Soil Samples ◽  
Spore Density ◽  
Wild Type ◽  
Isolation And Identification ◽  
Spore Count ◽  
Spore Number ◽  
Total Spore Count

Abstract. Mahulette AS, Alfian A, Kilkoda KA, Lawalata IJ, Marasabessy DA, Tanasale VL, Makaruku MH. 2021. Isolation and identification of indigenous Arbuscular Mycorrhizal Fungi (AMF) of forest clove rhizosphere from Maluku, Indonesia. Biodiversitas 22: 3613-3619. Forest clove is classified as wild-type and endemic to the Maluku (Moluccas) Islands, Indonesia. The different condition of growing areas causes various types of Arbuscular Mycorrhizal Fungi (AMF) associated with forest clove. The study aimed to identify and obtain indigenous AMF inoculums from the forest clove rhizosphere from two distribution areas in Maluku. The results of AMF identification found two types of spores from the genus Glomus in the rhizosphere of forest cloves from Ambon Island with a spore density of 35/50 g of soil. In comparison, three spores were found in Seram Island, two from the genus Scutellospora and one from the Acaulospora. With an overall spore density of 5/50 g of soil. After culture trapping, there was a change in type and an increase in spore density in soil samples from the rhizosphere of the two forest clove distribution areas. Soil samples from Ambon after trapping culture obtained two new types of spores from the genus Acaulospora with a total spore number of 57/50 g soil while in soil samples from Seram found three new types of spores from the genus Glomus with a total spore count of 104/50 g of soil.

scholarly journals NATIVE MICROBIOTA AND ARBUSCULAR MYCORRHIZAL FUNGI ON GROWTH OF Paspalum millegrana SCHRAD

2019 ◽  
Vol 32 (2) ◽  
pp. 345-353
Author(s):  
JOHNY DE JESUS MENDONÇA ◽  
LARISSA DE SOUZA GOIS ◽  
JACILENE FRANCISCA SOUZA SANTOS ◽  
TAMIRIS APARECIDA DE CARVALHO SANTOS ◽  
FRANCISCO SANDRO RODRIGUES HOLANDA ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Endophytic Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Mycorrhizal Colonization ◽  
Volume Number ◽  
The Family ◽  
Symbiotic Fungi ◽  
Shoot Mass ◽  
Gigaspora Albida

ABSTRACT Paspalum millegrana grass is a member of the family Poaceae native to the Americas, whose interaction with native symbiotic fungi has not yet been reported. The objective of this study was to evaluate the interactions between the native microorganisms and arbuscular mycorrhizal fungi in the development of P. millegrana Schrad. The experimental design was completely randomized with seven treatments (control, without AMF; native microbial inoculant; native + UFLA05 Gigaspora albida; native + UFLA351 Rhizoglomus clarum; native + UFLA372 Claroideoglomus etunicatum; native + UFLA401 Acaulospora morrowiae, and a mix of all treatments). The substrate was autoclaved sand and coconut powder at 2:1, with eight repetitions. The variables analyzed were: mycorrhizal colonization, dark septate endophytic fungi colonization, number of mycorrhizal spores, dry shoot mass, dry root mass, root length and volume, number of tiller and mycorrhizal dependence. Mycorrhizal arbuscular fungi and dark septate endophytic fungi colonized P. millegrana. The sporulation of arbuscular mycorrhizal fungi associated with P. millegrana was influenced by mycorrhizal colonization, depending on the fungus-plant interaction. P. millegrana was responsive to native + UFLA05 and native + UFLA351. No correlation between tiller emergence and mycorrhizal colonization of P. millegrana was observed.

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