Marker assisted stacking/pyramiding of stem rust, leaf rust and powdery mildew disease resistance genes (Sr2/Lr27/Yr30, Sr24/Lr24 and Sr36/Pm6) for durable resistance in wheat (Triticum aestivum L.)

2020 ◽  
Vol 11 (03) ◽  
1970 ◽  
Vol 21 ◽  
pp. 193-198
Author(s):  
I. O. Sozinov ◽  
N. O. Kozub ◽  
A. V. Karelov ◽  
L. A. Pylypenko ◽  
H. Ya. Bidnyk ◽  
...  

Aim. The aim of the study was to compare diversity of groups of winter common wheat varieties of the Steppe and the Central Forest-Steppe of Ukraine by storage protein loci and some disease resistance genes and to reveal peculiarities of varieties released after 1995. Methods. SDS and APAG electrophoresis was used to identify genotypes at the Glu-1, Gli-1, and some minor gliadin loci. PCR analysis was employed to study alleles of the disease resistance genes Lr34/Yr18/Pm38/Sr57/Bdv1, Tsn1, Tsc2, TDF_076_2D, and Cre-8. Results. Significant differences in frequencies of alleles at most marker loci were revealed. Nonrandom associations between disease resistance gene alleles as well as storage protein alleles were detected. Conclusions. The retention of a set of predominant alleles of a certain zone in different periods of breeding was confirmed. The appearance of new allele associations in the groups of varieties of the Steppe (in particular Gli-A1g and Glu-B1al) and the Central Forest-Steppe (1AL/1RS and Glu-B1d) in the last two decades was noted. Keywords: Triticum aestivum L., varieties, storage proteins, resistance genes, alleles.


2011 ◽  
Vol 59 (3) ◽  
pp. 241-248 ◽  
Author(s):  
G. Vida ◽  
M. Cséplő ◽  
G. Gulyás ◽  
I. Karsai ◽  
T. Kiss ◽  
...  

Among the factors which determine yield reliability an important role is played by disease resistance. One of the breeding aims in the Martonvásár institute is to develop wheat varieties with resistance to major diseases. The winter wheat varieties bred in Martonvásár are examined in artificially inoculated nurseries and greenhouses for resistance to economically important pathogens. The effectiveness of designated genes for resistance to powdery mildew and leaf rust has been monitored over a period of several decades. None of the designated major resistance genes examined in greenhouse tests is able to provide complete resistance to powdery mildew; however, a number of leaf rust resistance genes provide full protection against pathogen attack (Lr9, Lr19, Lr24, Lr25, Lr28 and Lr35). In the course of marker-assisted selection, efficient resistance genes (Lr9, Lr24, Lr25 and Lr29) have been incorporated into Martonvásár wheat varieties. The presence of Lr1, Lr10, Lr26, Lr34 and Lr37 in the Martonvásár gene pool was identified using molecular markers. New sources carrying alien genetic material have been tested for powdery mildew and leaf rust resistance. Valuable Fusarium head blight resistance sources have been identified in populations of old Hungarian wheat varieties. Species causing leaf spots (Pyrenophora tritici-repentis, Septoria tritici and Stagonospora nodorum) have gradually become more frequent over the last two decades. Tests on the resistance of the host plant were begun in Martonvásár four years ago and regular greenhouse tests on seedlings have also been initiated.


1985 ◽  
Vol 33 (2) ◽  
pp. 133-153 ◽  
Author(s):  
Jan Valkoun ◽  
Karl Hammer ◽  
Dagmar Kučerová ◽  
Pavel Bartoš

1977 ◽  
Vol 19 (4) ◽  
pp. 711-716 ◽  
Author(s):  
P. L. Dyck

The genetics of seedling resistance to leaf rust (Puccinia recondita Rob. ex. Desm.) was investigated in what (Triticum aestivum L.) introductions PI 268454, PI 58548 and PI 268316, originally collected in Afghanistan, China and Iran, respectively. PI 268454 was heterogeneous for resistance. A selection (PI 268454a) has a gene that confers a 1+ reaction while a second selection (PI 268454b) probably has resistance gene Lr2b. PI 58548 has two genes for resistance, one giving a 1+ reaction and the second a 2+. These two genes interact to produce a; 1 reaction. PI 268316 has three interacting genes, one giving a 1+ reaction, the second a 2+ and a third resistance gene similar to LrB. The gene giving the 1+ reaction was common to all three introductions. PI 58548 and PI 268316 carry different genes for infection type 2+. Backcross lines of the single genes were produced. Implications to breeding for disease resistance of genes interacting to produce different phenotype are discussed.


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