In Vitro Propagation of Heliconia psittacorum by Bud Culture

A protocol was developed for in vitro propagation of Heliconia psittacorum L.f. by culture of terminal and axillary buds of rhizomes. Cultures were initiated on modified Murashige and Skoog (MS) medium containing 40 μm BA, 150 ml coconut water/liter, 30 g sucrose/liter, and 2 g Gelrite/liter. Shoot multiplication was achieved on the above medium without coconut water, but supplemented with 10 μm BA. Shoots were rooted on MS basal medium and successfully acclimated to greenhouse conditions. Chemical names used: N-(phenylmethyl)-1 H-purin-6-amine (BA).

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In Vitro Propagation of Apios americana

HortScience ◽

10.21273/hortsci.25.11.1439 ◽

1990 ◽

Vol 25 (11) ◽

pp. 1439-1440 ◽

Cited By ~ 2

Author(s):

E.R.M. Wickremesinhe ◽

W.J. Blackmon ◽

B.D. Reynolds

Keyword(s):

Gibberellic Acid ◽

In Vitro Propagation ◽

Basal Medium ◽

Shoot Proliferation ◽

Axillary Buds ◽

Ms Medium ◽

Butanoic Acid ◽

Apios Americana

Shoot proliferation from axillary buds of Apios americana Medikus (apios, groundnut) was obtained on a modified Murashige and Skoog (MS) medium supplemented with 2.22 μm BAP, 0.5 μm IBA, and 3.0 μm GA3. Existed shoots rooted on MS basal medium. About 60% of the rooted plants were successfully established in soil. Chemical names used: 1 H-indole-3-butanoic acid (IBA). gibberellic acid (GA3), N6-benzylaminopurine (BAP).

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Micropropagation of Pyracantha coccinea

HortScience ◽

10.21273/hortsci11301-16 ◽

2017 ◽

Vol 52 (2) ◽

pp. 271-273

Author(s):

Chao Dong ◽

Xue Li ◽

Yue Xi ◽

Zong-Ming Cheng

Keyword(s):

In Vitro Propagation ◽

Basal Medium ◽

Ornamental Plant ◽

Axillary Buds ◽

Nodal Segments ◽

Ms Medium ◽

Southeast Europe ◽

Rooting Medium ◽

Pyracantha Coccinea

Pyracantha coccinea is a thorny evergreen shrub native to southeast Europe to southeast Asia. It is a popular ornamental plant because of its showy bright red fruits and small white flowers. However, in vitro vegetative propagation of P. coccinea has not been studied. Nodal segments with one or two axillary buds (1 to 1.5 cm in length) were cut and disinfected in a solution of 0.1% (v/v) mercuric chloride (HgCl2) for 5 minutes, and proliferated on Murashige and Skoog (MS) basal medium supplemented with various concentrations 6-benzylaminopurine (6-BA). After 4 weeks, newly formed shoots were transferred to proliferation and rooting media containing various concentrations of indole-3-butyric acid (IBA). Establishment of axillary buds was significantly better with an establishing rate of 67% on basal MS medium augmented with 6.6 µm 6-BA. The best medium for proliferation of shoots was three-fourth basal MS supplemented with 1.5 µm IBA, with a proliferation rate of 3.4 axillary bud. The optimum rooting medium was one-fourth MS basal medium containing 93 µm IBA. Rooting of shoots was as much as 77%. Rooted plantlets were transferred to pots containing vermiculite:perlite:peat (6:1:2) and acclimatized to ambient greenhouse conditions with a 95% survival rate. This protocol can be used for in vitro propagation of P. coccinea.

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In vitro propagation of the bay laurel (Laurus nobilis.L) in Morocco

South Asian Journal of Experimental Biology ◽

10.38150/sajeb.4(3).p96-103 ◽

2014 ◽

Vol 4 (3) ◽

pp. 96-103

Author(s):

Abdelali Chourfi ◽

Tajelmolk Alaoui ◽

Ghizlane Echchgadda

Keyword(s):

Seed Germination ◽

In Vitro Culture ◽

In Vitro Propagation ◽

Aerial Part ◽

Basal Medium ◽

Axillary Buds ◽

Laurus Nobilis

Laurus nobilis L. is among the species which are most threatened by massive degradation in Morocco. The multiplication by seed or by cuttings gives very low percentages of recovery that is insufficient to meet the demand of growing market. In vitro culture proves to be a tremendous asset to solve this problem. Our work has focused on the study of seed germination of this species and its multiplication from microcuttings. Finally, we studied the ac-climatization ability of the plantlets resulting from this germination. The study of the germination, via the further measurement of the length of the aerial part and the roots and the number of axillary buds for nine weeks, showed that the MS basal medium was more efficient than media 1/2M.S and WPM. Among the eight tested hormones, IAA yielded the best growth of the plantlets. Hormonal combination of NAA and kinetin resulted into a per-centage of the greatest success in reaching 67 % micropropagation. The study also revealed that the MS basal medium in the presence of the IAA plants can acclimate most easily in two types of substrates with improved development in the peat alone.

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Micropropagation of Cypripedium formosanum Hayata through Axillary Buds from Mature Plants

HortScience ◽

10.21273/hortsci.45.9.1369 ◽

2010 ◽

Vol 45 (9) ◽

pp. 1369-1372 ◽

Cited By ~ 5

Author(s):

Yung-I Lee

Keyword(s):

Average Length ◽

Basal Medium ◽

Shoot Multiplication ◽

Shoot Formation ◽

Axillary Buds ◽

Orchid Species ◽

Optimum Result ◽

Aseptic Culture ◽

Slipper Orchid

A micropropagation protocol for an endangered slipper orchid species, Cypripedium formosanum Hayata, through axillary buds from adult plants has been developed. The season of explant collection is crucial for the initial success of an aseptic culture. Explants collected in the middle of January gave the highest percentage of explant survival (54.2%) and shoot-forming percentage (41.7%). Of the two cytokinins tested, N6-benzyladenine (BA) was found to be superior to thidiazuron for normal shoot formation. The optimum result was obtained in quarter-strength Murashige and Skoog medium containing 22.2 or 44.4 μM BA in which the cultures produced 6.3 and 7.1 shoots per explant with 10.6 to 11.7 mm average length after 90 d of culture. Regenerated shoots rooted for 60 d in the basal medium with 1 g·L−1 activated charcoal and 20 g·L−1 potato homogenate were ready for growth in pots. This is the first report on shoot multiplication in vitro from mature plants of Cypripedium that provides a reliable method for propagating the selected elites.

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IN VITRO PROPAGATION OF ALGERIAN IVY (HEDERA CANARIENSIS L.)

HortScience ◽

10.21273/hortsci.25.9.1123g ◽

1990 ◽

Vol 25 (9) ◽

pp. 1123g-1124

Author(s):

Karim H. Al-Juboory ◽

David J. Williams

Keyword(s):

Root Length ◽

In Vitro Propagation ◽

Inverse Relationship ◽

Basal Medium ◽

Shoot Tip ◽

Ms Medium ◽

Shoot Tip Explants ◽

Strength Medium

Shoot tip explants of Algerian Ivy Heder a canariensis were cultured on MS basal medium supplemented with a combination of salt strength and NAA and IBA. More roots per explant developed on full salt strength medium combined with NAA. The most roots per explant were obtained with a combination of IBA and 1/4 MS salt. There was an inverse relationship between an increase in IBA or NAA concentration and root length and number. Shoots proliferated better on full MS salt combined with NAA and IBA. The highest level of NAA (40 uM) and 0.1 uM TDZ produced the most shoots and roots, the longest roots, the highest rooting percentage, the largest plants with the most leaves and the best callus quality per explant. The leaves from in vitro were cultured on MS medium with varying levels of Thidiazuron (TDZ) and NAA in the presence of light produced the highest number of roots.

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In vitro propagation of popular banana cultivar (Musa spp. Cv. Patakpura)

Bangladesh Journal of Agricultural Research ◽

10.3329/bjar.v44i4.45699 ◽

2020 ◽

Vol 44 (4) ◽

pp. 641-648

Author(s):

Bandita Deo ◽

Bikram Keshari ◽

Bikram Pradhan

Keyword(s):

Acetic Acid ◽

In Vitro Propagation ◽

Indole Acetic Acid ◽

Shoot Multiplication ◽

Naphthalene Acetic Acid ◽

Ms Medium ◽

Musa Sp ◽

Initial Culture ◽

Banana Cultivar

The present experiment was conducted to optimize protocols for in vitro propagation of banana (Musa sp.) cv. ‘Patakpura’ (AAB), supplemented with different growth regulators. Shoot tips obtained from sword suckers were cultured aseptically on MS medium supplemented with different concentrations of cytokinins like 6-Benzylaminopurine (BAP) and Kinetin (KN) for multiplication of shootsand auxins such as indole acetic acid (IAA) and naphthalene acetic acid (NAA) for induction of roots. The best result from the initial culture was obtained from MS medium supplimented with 4 mg/l BAP + 0.5 mg/l IAA. The highest shoot fresh weight, shoot length and number of shoots per explant were recorded from MS medium supplemented with 4 mg/l BAP + 0.5 mg/l IAA + 0.25 mg/l NAA. Therefore, the MS medium supplemented with 4 mg/l BAP + 0.5 mg/l IAA + 0.25 mg/l NAA was found to be most effective and productive combination for shoot multiplication and proliferation of the culture in vitro. IAA at a concentration of 1 mg/l was found to be most suitable for rooting of the shoots. Bangladesh J. Agril. Res. 44(4): 641-648, December 2019

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In vitro propagation of Gentiana dinarica Beck.

Open Life Sciences ◽

10.2478/s11535-012-0059-7 ◽

2012 ◽

Vol 7 (4) ◽

pp. 690-697 ◽

Cited By ~ 4

Author(s):

Branka Vinterhalter ◽

Dijana Milošević ◽

Teodora Janković ◽

Jelena Milojević ◽

Dragan Vinterhalter

Keyword(s):

Plant Growth Regulator ◽

In Vitro Propagation ◽

Shoot Multiplication ◽

Ms Medium ◽

Root Initiation ◽

Free Medium ◽

Rare And Endangered Species ◽

Rare And Endangered ◽

Gentiana Dinarica

AbstractGentiana dinarica Beck, rare and endangered species of Balkan Dinaric alps, was in vitro propagated (micropropagated) from axillary buds of plants collected at Mt. Tara, Serbia. G. dinarica preferred MS to WPM medium, with optimal shoot multiplication on MS medium with 3% sucrose, 1.0 mg l−1 BA and 0.1 mg l−1 NAA. Rooting was not clearly separated from shoot multiplication since BA did not completely inhibit root initiation. Spontaneous rooting on plant growth regulator-free medium occurred in some 30% of shoot explants. Rooting was stimulated mostly by decreased mineral salt nutrition and a medium with 0.5 MS salts, 2% sucrose and 0.5–1.0 mg l−1 IBA was considered to be optimal for rooting. Rooted plantlets were successfully acclimated and further cultured in peat-based substrate.

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Fast protocol for high frequency in vitro cloning of Banana (Musa acuminata) cv. Grande Naine

Journal of Applied and Natural Science ◽

10.31018/jans.v9i1.1153 ◽

2017 ◽

Vol 9 (1) ◽

pp. 72-79

Author(s):

S. R. Parida ◽

S. Beura ◽

S. Rout ◽

R. Beura ◽

P. N. Jagadev

Keyword(s):

High Frequency ◽

Basal Medium ◽

Shoot Multiplication ◽

Musa Acuminata ◽

Future Research ◽

Naphthaleneacetic Acid ◽

Maximum Height ◽

Ms Medium ◽

Number Of Leaves

An investigation was conducted on Fast Protocol for High Frequency in vitro cloning of Banana (Musa acuminata) cv. Grande Naine at the Biotechnology-cum-Tissue Culture Center, OUAT, Bhubaneswar, during the year 2012. This has helped to determine the best media compositions for shoot multiplication and rooting of cv. Grande Naine, so as to get optimum results with a minimized cost of production. MS medium supplemented with 4.0 mg/1 Benzylaminopurine (BAP) and 2.0 mg/1 Kinetin gave the highest number of shoot/explants (11.33) in 30 days. However, MS medium when supplemented with 6.0 mg/1 BAP produced a maximum number of leaves (19.07) with a maximum height 2.73 cm. Among various concentrations of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA) for rooting. Half MS medium supplemented with 1.0 mg/1 IBA was found to be ideal for early rooting and producing more number of roots in 21 days. However, MS basal medium was found to be the best treatment to support the formation of long roots. This protocol can be very useful to the future research worker and as well as entrepreneurs for mass production of banana (Musa acuminata) cv. Grande Naine.

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Plant Regeneration from Leaf Explants of the Medicinal Herb Wedelia chinensis

Horticulturae ◽

10.3390/horticulturae7100407 ◽

2021 ◽

Vol 7 (10) ◽

pp. 407

Author(s):

Yung-Ting Tsai ◽

Kin-Ying To

Keyword(s):

Plant Regeneration ◽

In Vitro Propagation ◽

Liver Toxicity ◽

Leaf Explants ◽

Basal Medium ◽

Nodal Segments ◽

Naphthaleneacetic Acid ◽

Ms Medium ◽

Average Percentage

Wedelia chinensis, belonging to the Asteraceae family, has been used in folk medicine in East and South Asia for the treatment of common inflammatory diseases and protection against liver toxicity. Previously, in vitro propagation through different tissue explants has been reported, including through nodal segments, axillary buds, and shoot tips, whereas leaf segments failed to proliferate. Here, we report on the in vitro propagation of W. chinensis by culturing young leaf explants in MS medium supplemented with 0.5 mg/L α-naphthaleneacetic acid (NAA), 0.75 mg/L thidiazuron (TDZ), 1 mg/L gibberellic acid (GA3), 3.75 mg/L adenine, 3% sucrose, and 0.8% agar at pH 5.8. Calli were observed in all explants derived from the youngest top two leaves, and the average percentage of shoot regeneration was 23% from three independent experiments. Then, several shoots were excised, transferred onto MS basal medium supplemented with 3% sucrose and 0.8% agar at pH 5.8, and cultured in a growth chamber for 1 to 2 months. Roots were easily induced. Finally, plantlets carrying shoots and roots were transferred into soil, and all of them grew healthily in a greenhouse. No morphological variation was observed between the regenerated plantlets and the donor wild-type plants. In addition, we also established root cultures of W. chinensis in culture medium (MS medium, 3 mg/L NAA, 3% sucrose, pH 5.8) with or without 0.8% agar. To the best of our knowledge, this is the first paper reporting plant regeneration from leaf explants in the herbal plant W. chinensis.

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Micropropagation of Baptisia `Purple Smoke'

HortScience ◽

10.21273/hortsci.34.2.353 ◽

1999 ◽

Vol 34 (2) ◽

pp. 353-354 ◽

Cited By ~ 1

Author(s):

James R. Ault ◽

Kayri Havens

Keyword(s):

Plant Growth Regulator ◽

Potassium Salt ◽

Shoot Growth ◽

Basal Medium ◽

Shoot Multiplication ◽

Naphthalene Acetic Acid ◽

Ms Medium ◽

Single Node ◽

Shoot Initiation

Shoot explants from actively growing, greenhouse-maintained plants of Baptisia `Purple Smoke' were cultured in vitro for shoot initiation on Murashige and Skoog (MS) basal medium containing vitamins and supplemented with 30 g·L–1 sucrose, 8.87 μm BA, and 4.14 μm K-IBA. All subsequent media were supplemented with 2.47 mm NaH2PO4 to enhance shoot growth. Single-node explants were subcultured for shoot multiplication on MS medium with either no plant growth regulator or with 2.22, 4.44, 8.87, 17.74, or 35.48 mm BA in combination with 0.0 or 4.14 μm K-IBA. Explants produced a maximum of 4.1 shoots on the medium with 2.22 μm BA. Shoots rooted on all concentrations of K-IBA (2.07, 4.14, 10.36, or 20.72 μm) and K-NAA (2.23, 4.46, 11.15, or 22.29 μm) tested. Maximum rooting was 100% on MS medium with 11.15 μm K-NAA; however, this treatment induced copious stem callusing. Rooted shoots were greenhouse-acclimatized for 2.5 weeks. Overall survival was 86%. For optimal rooting and subsequent acclimatization, treatment with 2.23 μm K-NAA is recommended; this resulted in 83% rooting and 87% acclimatization. Chemical names used: N6 benzyladenine (BA); potassium salt of indole-3-butyric acid (K-IBA); potassium salt of 1-naphthalene acetic acid (K-NAA).

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