An improved pre-culture procedure for the detection of Salmonella in frozen chicken meat by duplex polymerase chain reaction

Arcobacters are food and waterborne pathogens associated with human and animal infections. The objective of the present study was to investigate the prevalence and diversity of Arcobacter spp. in commercially sold chicken meat in İzmir region of Turkey. For this purpose, 100 samples including legs (n=40), 17 chicken quarters (n=17), drumstickers (n=16), breasts (n=11), wings (n=10), and carcasses (n=6) were collected from different retail markets. A total of 65 isolates were confirmed as Arcobacter spp. from 55 samples by genus-specific polymerase chain reaction (PCR). The prevalence of Arcobacter spp. was 32.5, 81.3, 64.7, 72.7, 83.3, and 50% for legs, drumstickers, chicken quarters, breasts, carcasses and wings, respectively. Based on the multiplex-PCR, most of the isolates were identified as A. butzleri (n=45, 80%), followed by A. cryaerophilus (n=2, 3.6%), A. skirrowii (n=1, 1.8%) and 17 isolates (30.9%) could not be identified at the species level.

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Molecular characterization of Candida dubliniensis and Candida albicans in the oral cavity of drug abusers using duplex polymerase chain reaction

Current Medical Mycology ◽

10.18502/cmm.4.1.29 ◽

2018 ◽

Vol 4 (1) ◽

Cited By ~ 1

Author(s):

Parastoo Hassani Abharian ◽

Parvin Dehghan ◽

Peyman Hassani Abharian ◽

Sepideh Tolouei

Keyword(s):

Polymerase Chain Reaction ◽

Candida Albicans ◽

Oral Cavity ◽

Candida Species ◽

Species Complex ◽

Candida Dubliniensis ◽

Drug Abusers ◽

Chain Reaction ◽

Polymerase Chain ◽

Duplex Polymerase Chain Reaction

Background and Purpose: Candida dubliniensis is closely related to the most pathogenic and prevalent yeast, namely C. albicans. Candida species can opportunistically overgrow in vulnerable individuals and cause a variety of diseases. The current study aimed to identify and isolate C. dubliniensis species present in the Candida albicans species complex identified in the oral cavity of drug abusers. Materials and Methods: This study was conducted on 53 strains of C. albicans species complex, isolated from the oral mucosa of drug abusers in Isfahan, Iran. DNA extraction was accomplished through boiling procedure. Duplex polymerase chain reaction (PCR) was performed to amplify ITS1-5.8S-ITS2 region using four specific primers. Fungal species were identified based on the difference in the size of the bands created in the Agarose gel. Results: Out of the 53 isolates under study, 30 (56.6%) and 14 (26.4%) samples were identified as C. albicans and C. dubliniensis, respectively. In the remaining 9 samples (17%), both types of Candida species were confirmed. Conclusion: The findings of the present study revealed the presence of a noticeable amount of C. dubliniensis in the oral cavity of drug abusers. Therefore, the probable presence of this fungus should be considered during the examination of oral infection among this group. To date, no research has directly investigated this issue in Iran.

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