scholarly journals DUPLEX POLYMERASE CHAIN REACTION WITH CONFRONTING TWO-PAIR PRIMERS (PCR-CTPP) FOR GENOTYPING ALCOHOL DEHYDROGENASE   SUBUNIT (ADH2) AND ALDEHYDE DEHYDROGENASE 2 (ALDH2)

2003 ◽  
Vol 38 (5) ◽  
pp. 407-410 ◽  
Author(s):  
A. Tamakoshi ◽  
N. Hamajima ◽  
H. Kawase ◽  
K. Wakai ◽  
N. Katsuda ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Alcohol Dehydrogenase ◽  
Aldehyde Dehydrogenase ◽  
Aldehyde Dehydrogenase 2 ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Duplex Polymerase Chain Reaction

scholarly journals TaqMan Real-Time Polymerase Chain Reaction and Pyrosequencing using Single Nucleotide Polymorphism Protocol for Rapid Determination of ALDH2 *2 in a Chinese Population

2015 ◽  
Vol 14 (9) ◽  
pp. 1679-1684
Author(s):  
J Li ◽  
J Wu ◽  
Y Zhang ◽  
X Wang ◽  
J Jin ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Aldehyde Dehydrogenase ◽  
Real Time Pcr ◽  
Chinese Population ◽  
Allele Frequencies ◽  
Genotype Frequency ◽  
Aldehyde Dehydrogenase 2 ◽  
Chain Reaction ◽  
Polymerase Chain

Purpose: To establish a rapid molecular method for the detection of aldehyde dehydrogenase 2 (ALDH2), and determine whether the polymorphic ALDH2 gene is associated with drinking behavior in a Chinese population.Methods: The gene polymorphism of ALDH2 *2 was detected using pyrosequencing and TaqMan realtime polymerase chain reaction (PCR) techniques. Genotyping of 176 volunteers were performed at The Third Hospital of Wuhan, Hubei, China. Genetic associations with alcohol use behavior was assessed.Results: Pyrosequencing and TaqMan real time PCR methods were successfully developed to identify ALDH2 *2 polymorphisms. The allele frequencies of ALDH2 *2 were 21.31 % in the Chinese population. While the genotype frequency of heterozygous ALDH2 *1/ *2 plus homozygous ALDH2 *2/ *2 was 46.67 % in non-drinkers, it was 30.17 % in the alcoholics group (p = 0.030). The allele frequency of ALDH2 *2 was 15.09 % in the alcoholics group and 28.33 % among non-drinkers (p = 0.011). There were  substantial differences in allele frequencies of ALDH2 genotypes between this study’s Chinese sample and other ethnic groups in Asia; the frequency of ALDH2 *2 allele was significantly higher than that in European and Americans.Conclusion: The developed pyrosequencing and TaqMan real time PCR methods are rapid, accurate, high-throughput, convenient, and reliable for detecting ALDH2 polymorphisms. ALDH2 *2 gene protects against the development of alcoholism. The frequencies of ALDH2 in this Chinese population differ from those of other  ethnic populations.Keywords: Aldehyde dehydrogenase 2, Polymorphism, TaqMan real time Polymerase Chain Reaction, Pyrosequencing, Allele, Alcoholics, Genotype frequency

scholarly journals Molecular characterization of Candida dubliniensis and Candida albicans in the oral cavity of drug abusers using duplex polymerase chain reaction

2018 ◽  
Vol 4 (1) ◽  
Author(s):  
Parastoo Hassani Abharian ◽  
Parvin Dehghan ◽  
Peyman Hassani Abharian ◽  
Sepideh Tolouei
Keyword(s):  
Polymerase Chain Reaction ◽  
Candida Albicans ◽  
Oral Cavity ◽  
Candida Species ◽  
Species Complex ◽  
Candida Dubliniensis ◽  
Drug Abusers ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Duplex Polymerase Chain Reaction

  Background and Purpose: Candida dubliniensis is closely related to the most pathogenic and prevalent yeast, namely C. albicans. Candida species can opportunistically overgrow in vulnerable individuals and cause a variety of diseases. The current study aimed to identify and isolate C. dubliniensis species present in the Candida albicans species complex identified in the oral cavity of drug abusers. Materials and Methods: This study was conducted on 53 strains of C. albicans species complex, isolated from the oral mucosa of drug abusers in Isfahan, Iran. DNA extraction was accomplished through boiling procedure. Duplex polymerase chain reaction (PCR) was performed to amplify ITS1-5.8S-ITS2 region using four specific primers. Fungal species were identified based on the difference in the size of the bands created in the Agarose gel. Results: Out of the 53 isolates under study, 30 (56.6%) and 14 (26.4%) samples were identified as C. albicans and C. dubliniensis, respectively. In the remaining 9 samples (17%), both types of Candida species were confirmed. Conclusion: The findings of the present study revealed the presence of a noticeable amount of C. dubliniensis in the oral cavity of drug abusers. Therefore, the probable presence of this fungus should be considered during the examination of oral infection among this group. To date, no research has directly investigated this issue in Iran.

Fetal Rhesus D genotyping on amniocytes in alloimmunised pregnancies using fluorescence duplex polymerase chain reaction

1997 ◽  
Vol 104 (1) ◽  
pp. 15-19 ◽  
Author(s):  
+Gerd Crombach ◽  
Frauke Picard ◽  
Matthias Beckmann ◽  
Boris Tutschek Resident ◽  
Reiner Bald ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Duplex Polymerase Chain Reaction

Rapid identification of Fonsecaea by duplex polymerase chain reaction in isolates from patients with chromoblastomycosis

2007 ◽  
Vol 57 (3) ◽  
pp. 267-272 ◽  
Author(s):  
Tânia Sueli de Andrade ◽  
Arlete Emily Cury ◽  
Luiz Guilherme Martins de Castro ◽  
Mario Hiroyuki Hirata ◽  
Rosario Dominguez Crespo Hirata
Keyword(s):  
Polymerase Chain Reaction ◽  
Rapid Identification ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Duplex Polymerase Chain Reaction

scholarly journals Association study of the Ile349val polymorphism of the gene ADH1C and alcohol dependence

2011 ◽  
Vol 60 (1) ◽  
pp. 7-10 ◽  
Author(s):  
André Soares Rebello ◽  
Rodrigo Moura-Neto ◽  
Maria da Glória da Costa Carvalho
Keyword(s):  
Polymerase Chain Reaction ◽  
Alcohol Dehydrogenase ◽  
Alcohol Dependence ◽  
Alcoholics Anonymous ◽  
Control Group ◽  
Chain Reaction ◽  
Alcohol Dependence Syndrome ◽  
Genotype Difference ◽  
Polymerase Chain ◽  
Janeiro City

OBJECTIVE: The aim of this study was to investigate the polymorphism Ile349Val of the enzyme alcohol dehydrogenase ADH1C gene among individuals with alcohol dependence syndrome (ADS) attending Alcoholics Anonymous (AA) meetings. METHODS: A total of 120 subjects residing in Rio de Janeiro city participated in this study. Subjects were divided into two groups: a group consisting of 54 individuals from the ADS group and 66 individuals that declared not having any alcohol dependence (control group). DNA was extracted from mouth epithelial cells by phenol-chloroform method and further submitted to amplification by polymerase chain reaction (PCR). RESULTS: Our results did not show differences between the genotypes of control individuals and ADS subjects. Nevertheless, we found increased rates of alcoholism in families of ADS subjects as compared to controls. CONCLUSIONS: Our results did not show any genotype difference on the ADH1C gene when control and AA genotypes are compared.

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