Aspen produces large numbers of seeds, even though it mainly reproduces asexually with root suckers. The aim of this study was to find out how different moisture conditions affect emergence and survival of Populus tremula L. seedlings. This was studied with a sowing experiment (totally randomized factorial design). There were altogether 10 blocks, each containing 16 microsites and three treatments (sowing time, watering, sowing shelter) replicated twice in each block. Seedlings emerged on 56% of microsites. Sowing time affected seedling emergence. Both the proportion of microsites with seedlings and the number of seedlings per microsite were lower after first than after second sowing, when the weather was rainier. Watering increased the number of seedlings per microsite, but the proportion of micro sites with at least one seedling was not affected. Sowing shelter had a negative effect on the seedling emergence, especially after second sowing. The survival of seedlings was low (10%) and strongly dependent on watering. The effect of block and its interactions with treatments indicated that seedling emergence and survival depended also on seedbed conditions. We conclude that sexual reproduction of aspen may occur in nature, but it is rare. The seeds also maintained their germinability longer than earlier observed.