scholarly journals Detection and genotyping of Leptospira spp. from the kidneys of a seemingly healthy pig slaughtered for human consumption

2015 ◽  
Vol 9 (05) ◽  
pp. 530-532 ◽  
Author(s):  
Ashutosh Verma ◽  
Esteban Soto ◽  
Oscar Illanes ◽  
Souvik Ghosh ◽  
Carmen Fuentealba
Keyword(s):  
Quantitative Pcr ◽  
Multilocus Sequence Typing ◽  
Human Consumption ◽  
Caribbean Island ◽  
Pathogenic Leptospira ◽  
Zoonotic Pathogen ◽  
Leptospira Spp ◽  
Swine Population ◽  
The Caribbean

Introduction: Leptospirosis is a zoonotic disease caused by pathogenic Leptospira spp.  Leptospirosis is maintained in an environment due to chronic kidney infection of a wide variety of domestic, peridomestic and wild reservoir mammals. In this study the role of pigs in maintenance of leptospires on the Caribbean island of St. Kitts was investigated. Methodology: The condemned kidneys of 60 pigs slaughtered at a St. Kitts abattoir were screened by a quantitative-PCR for the presence of Leptospira spp. Positive samples were genotyped using a six-gene based multilocus sequence typing scheme. Results: Leptospiral DNA was detected in the kidneys of one of the 60 pigs. Multilocus sequence typing identified the infecting species to be L. interrogans. Conclusions: Detection of this zoonotic pathogen in the kidneys of a seemingly healthy pig raises concerns regarding the subclinical carriers of the disease among the island’s swine population.

scholarly journals A quantitative PCR (TaqMan) assay for pathogenic Leptospira spp

2002 ◽  
Vol 2 (1) ◽  
Author(s):  
Lee D Smythe ◽  
Ina L Smith ◽  
Greg A Smith ◽  
Michael F Dohnt ◽  
Meegan L Symonds ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Taqman Assay ◽  
Pathogenic Leptospira ◽  
Leptospira Spp

scholarly journals Vulnerability and Resilience in the Caribbean Island States; the Role of Connectivity

2021 ◽  
Author(s):  
Edwina E. Pereira ◽  
Albert E. Steenge
Keyword(s):  
Important Variable ◽  
Small States ◽  
Caribbean Island ◽  
Island States ◽  
Caribbean Area ◽  
The Caribbean ◽  
The One ◽  
Dutch Caribbean ◽  
Political Economic

AbstractIt is well-known that small states, because of their size, tend to be less endowed with natural resources than big ones. This makes small states vulnerable and raises the question if specific policies can be implemented to offset the drawbacks of their small size and to increase resilience. We address this question in this paper, thereby focusing on the role of connectivity – between states, organisations, parties, or otherwise – in understanding a country’s vulnerability and resilience. Here ‘policies’ are interpreted as ‘institutions’ in the sense of Douglass C. North (1990), i.e. as ‘humanly devised constraints that structure political, economic and social interaction’. We focus on the Caribbean area, which is characterised by a wide variety of small states, each with its own set of rules and regulations. Within this area, we concentrate on the relationship between three Dutch Caribbean islands, i.e., Aruba, Curaçao, and Sint Maarten, on the one hand, and the Netherlands, the former colonizer, on the other hand. As a first step we have measured the economic vulnerability and resilience of 17 Caribbean island states, both dependent and independent, employing the theoretical framework proposed by Lino Briguglio. The outcomes show that the three Dutch island states are performing comparatively well, although there are individual differences. We provide a first effort to explain this outcome in terms of the continuing interest of the three island states to keep their ties to the former colonizer viable. Here the presence of ‘systemic interest’ as shown by the stakeholders appears to be a most important variable.

On your knees and pray! The role of religion in the development of a metal scene in the Caribbean island of Puerto Rico

2014 ◽  
Vol 7 (2) ◽  
pp. 243-257
Author(s):  
Nelson Varas-Díaz ◽  
Eliut Rivera-Segarra ◽  
Sigrid Mendoza ◽  
Osvaldo González-Sepúlveda
Keyword(s):  
Puerto Rico ◽  
Caribbean Island ◽  
The Caribbean ◽  
Role Of Religion

scholarly journals Bats are a potential reservoir of pathogenic Leptospira species in Colombia

2019 ◽  
Vol 13 (04) ◽  
pp. 278-283 ◽  
Author(s):  
Jose Mateus ◽  
Natalia Gómez ◽  
María Teresa Herrera-Sepúlveda ◽  
Marylin Hidalgo ◽  
Jairo Pérez-Torres ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Potential Role ◽  
Caribbean Region ◽  
Pathogenic Leptospira ◽  
Glossophaga Soricina ◽  
Leptospira Spp ◽  
Potential Reservoir ◽  
Causative Agents ◽  
The Caribbean ◽  
Leptospira Species

Introduction: Bats have become an epidemiologically significant source of pathogenic microorganisms, such as leptospires, the causative agents of leptospirosis. However, little information exists about bats and their potential role as a reservoir of pathogenic Leptospira spp. in Colombia. The aim of this study was to evaluate the presence of pathogenic Leptospira spp. in the kidneys of bats from the Caribbean region of Colombia deposited in the collection of mammals of the Museo Javeriano de Historia Natural (MPUJ-MAMM). Methodology: DNA was extracted from twenty-six kidney samples from a total of 13 species of bats captured in Colombia. First, 16S ribosomal RNA conventional PCR was performed to detect the presence of Leptospira spp. Then, in samples that tested positive, LipL32 PCR was performed to detect pathogenic Leptospira spp. by sequencing and phylogenetic analysis. Results: The presence of Leptospira spp. was observed in 7/26 (26.9%) bats from the following 6 species: Carollia perspicillata, Glossophaga soricina, Dermanura phaeotis, Uroderma bilobatum, Desmodus rotundus, and Lophostoma silvicolum, and pathogenic Leptospira spp. were detected in 4/26 samples (15.4%). Conclusions: This study suggests that bats present in the Caribbean region of Colombia could be potential reservoirs of pathogenic Leptospira spp.

scholarly journals Subtyping Salmonella isolated from pet dogs with multilocus sequence typing (MLST) and clustered regularly interspaced short palindromic repeats (CRISPRs)

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Cheng Yang ◽  
Wangfeng Shao ◽  
Lingling Wei ◽  
Lingxiao Chen ◽  
Aihua Zhu ◽  
...  
Keyword(s):  
Multilocus Sequence Typing ◽  
Sequence Type ◽  
Jiangsu Province ◽  
Zoonotic Pathogen ◽  
Recognition Ability ◽  
Prevention And Treatment ◽  
Common Serotypes ◽  
The Common ◽  
Different Sources ◽  
Positive Effect

AbstractSalmonella, as a zoonotic pathogen, has attracted widespread attention worldwide, especially in the transmission between household pets and humans. Therefore, we investigated the epidemic distribution of dog Salmonella from pet hospitals and breeding base in Xuzhou, Jiangsu Province, China, and used multilocus sequence typing (MLST) and clustered regularly interspaced short palindromic repeats (CRISPRs) to subtype Salmonella isolates. From April 2018 to November 2019, a total of 469 samples were collected from pet hospitals and breeding base, including 339 dog samples and 60 cat samples. S. Kentucky (40.74%) was the most prevalent serotype, but other, such as S. Typhimurium (18.52%) and S. Indiana (18.52%), were also widespread. Eight different sequence type (ST) patterns were identified by MLST and ST198 was the highest proportion of these isolates. CRISPRs analysis showed that 9 different Kentucky CRISPR types (KCTs) was identified from ST198. 48 spacers including 29 (6 News) for CRISPR1 and 19 (4 News) for CRISPR2 that proved the polymorphic of Salmonella genes in samples from different sources. The analysis demonstrated that the common serotypes were widely present in pet hosts in the same area. This analysis shows that CRISPR genes have better recognition ability in the same serotype, which has a positive effect on the traceability of Salmonella and the prevention and treatment of salmonellosis.

Detection and characterization of Leptospira spp. in dogs diagnosed with kidney and/or liver disease in Selangor, Malaysia

2021 ◽  
pp. 104063872110245
Author(s):  
Sabri A. Rahman ◽  
Kuan H. Khor ◽  
Siti Khairani-Bejo ◽  
Seng F. Lau ◽  
Mazlina Mazlan ◽  
...  
Keyword(s):  
Liver Disease ◽  
Mortality Rate ◽  
Direct Detection ◽  
Bacterial Disease ◽  
Pathogenic Leptospira ◽  
Wide Range ◽  
Leptospira Spp ◽  
Rrna Sequencing ◽  
The Common

Leptospirosis is a serious bacterial disease that affects both humans and animals. A wide range of symptoms have been described in humans; the disease in dogs is commonly associated with kidney and/or liver disease. In Malaysia, information about the common serovars infecting dogs is limited. Therefore, we investigated the occurrences of leptospirosis in 124 pet dogs diagnosed with kidney and/or liver disease. Blood, urine, abdominal effusion, and/or kidney and liver were collected from the dogs. Based on microscopic agglutination testing, 53 of 124 (42.7%) dogs were seropositive for leptospiral exposure. Sera were frequently positive to serovars Bataviae ( n = 12), Javanica ( n = 10), and Icterohaemorrhagiae ( n = 10). Direct detection using PCR showed that 42 of 124 (33.9%) of the whole blood and 36 of 113 (31.9%) urine samples were positive for pathogenic Leptospira spp. By PCR, 2 of 23 (9.1%) kidney and 2 of 23 (9.1%) liver were positive for pathogenic Leptospira spp. Abdominal effusion from 4 dogs were PCR-positive for pathogenic Leptospira spp. The species detected were L. interrogans, L. borgpetersenii, L. kirschneri, and L. kmetyi by partial 16S rRNA sequencing. We further identified and characterized 11 Leptospira spp. isolates from 8 dogs as serovars Bataviae, Javanica, and Australis. The mortality rate of the Leptospira-infected dogs was high (18 of 53; 34%).

Protein typing of major outer membrane lipoproteins from Chinese pathogenic Leptospira spp. and characterization of their immunogenicity

Vaccine ◽  
2009 ◽  
Vol 28 (1) ◽  
pp. 243-255 ◽  
Author(s):  
Dongjiao Luo ◽  
Feng Xue ◽  
David M. Ojcius ◽  
Jinfang Zhao ◽  
Yafei Mao ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Pathogenic Leptospira ◽  
Leptospira Spp ◽  
Membrane Lipoproteins
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