One Health Genomic Study of Human and Animal Klebsiella pneumoniae Isolated at Diagnostic Laboratories on a Small Caribbean Island

Klebsiella pneumoniae causes a variety of infections in both humans and animals. In this study, we characterised the genomes of human and animal isolates from two diagnostic laboratories on St. Kitts, a small Caribbean island inhabited by a large population of vervet monkeys. In view of the increased chances of direct or indirect contact with humans and other animal species, we used the One Health approach to assess transmission of K. pneumoniae across host species by sequencing 82 presumptive K. pneumoniae clinical isolates from humans (n = 51), vervets (n = 21), horses (n = 5), dogs (n = 4) and a cat (n = 1). Whole genome sequencing (WGS) was carried out using Illumina technology. De novo assembly was performed in CLC Genomics Workbench v.11.0. Single nucleotide polymorphisms were detected using NASP followed by phylogenetic analysis using IQ-TREE. Virulence and antimicrobial resistance gene contents were analysed using the Kleborate and CGE pipelines. WGS-based analysis showed that 72 isolates were K. pneumoniae sensu stricto and five K. quasipneumoniae and five K. variicola. K. pneumoniae isolates belonged to 35 sequence types (ST), three of which were occasionally shared between humans and animals: ST23, ST37 and ST307. The ST23 strains from vervets formed a separate cluster amongst publicly available sequenced ST23 strains, indicating the presence of a specific vervet sublineage. Animal strains harbored fewer resistance genes and displayed distinct virulence traits that appeared to be host-specific in vervet isolates. Our results show that K. pneumoniae infections on this Caribbean island are usually caused by host-specific lineages.

Mapping the economic loss of ecosystem services caused by the invasive plant species Antigonon leptopus on the Dutch Caribbean Island of St. Eustatius

Invasive species are a worldwide threat to biodiversity, especially on Caribbean islands. Through their impact on the structure and functioning of ecosystems, they also affect ecosystem services. Therefore, invasive species can have profound socio-economic effects. On the Dutch Caribbean Island of St. Eustatius, the invasive perennial vine Coralita is present on roughly 33% of the Island. While ecological damage is evident, effective management strategies are still lacking. This study links the ecological, cultural and societal effects of the invasion to the economy of the Island by estimating the ecosystem service losses due to Coralita in monetary value. We have spatially assessed the economic value of five main ecosystem services (tourism, non-use value, carbon sequestration, archaeology and local cultural and recreational value) to the different habitats on the Island and estimated the loss of these values under three scenarios of Coralita cover: 0%, 3% and 36% dominant cover. The baseline scenario of 0% demonstrated a total ecosystem service value of $2.7 million per year, concentrated on the Quill volcano. The 3% and 36% scenario showed a yearly loss of $39,804 and $576,704, respectively, with the largest losses located on the northern and eastern slopes of the Quill. These areas should be prioritised for management and the known potential gain per area enables choice of strategy, based on cost-benefit considerations. To reduce further economic loss by Coralita, we urgently advise an immediate management strategy and ongoing research into eradication and restoration methods.

Energy-Efficient Building Design for a Tropical Climate: A Field Study on the Caribbean Island Curaçao

Based on an extensive literature review on passive building designs for tropical climates, seven energy-efficient building design principles for tropical climate areas were deduced. These are: 1. To orientate a building design in such a direction that it protects from excessive solar radiation; 2. To accommodate for indoor natural ventilation; 3. That it makes maximal use of indirect instead of direct natural light; 4, That it reduces the amount of heat transmission through the roof as much as possible by natural ventilation between roof and ceiling and by lowering the roof surface temperature; 5. By preventing the use of high thermal mass materials; 6. By reducing through the exterior walls as much as possible heat transmission by e.g., preventing direct sunlight on the external walls and applying reflective paints on the external walls and; 7. By creating outdoor and transition spaces such as balconies, terraces atriums and corridors. The insights from the literature review were used as input to conduct a field study to evaluate the practice of applying passive building design principles. To this end, for 626 buildings on the Caribbean island Curaçao, it was investigated to what extent the recommended passive design principles for tropical climates were actually applied. Based on the results of the field study, several recommendations are made to improve the practice of applying passive building principles.

Antigonon leptopus invasion is associated with plant community disassembly in a Caribbean island ecosystem

Invasions by non-native plant species are widely recognized as a major driver of biodiversity loss. Globally, (sub-)tropical islands form important components of biodiversity hotspots, while being particularly susceptible to invasions by plants in general and vines in particular. We studied the impact of the invasive vine A. leptopus on the diversity and structure of recipient plant communities on the northern Caribbean island St. Eustatius. We used a paired-plot design to study differences in species richness, evenness and community structure under A. leptopus-invaded and uninvaded conditions. Community structure was studied through species co-occurrence patterns. We found that in plots invaded by A. leptopus, species richness was 40–50% lower, and these plots also exhibited lower evenness. The magnitude of these negative impacts increased with increasing cover of A. leptopus. Invaded plots also showed higher degrees of homogeneity in species composition. Species co-occurrence patterns indicated that plant communities in uninvaded plots were characterized by segregation, whereas recipient plant communities in invaded plots exhibited random co-occurrence patterns. These observations suggest that invasion of A. leptopus is not only associated with reduced species richness and evenness of recipient communities in invaded sites, but also with a community disassembly process that may reduce diversity between sites. Given that A. leptopus is a successful invader of (sub-)tropical islands around the globe, these impacts on plant community structure highlight that this invasive species could be a particular conservation concern for these systems.

Major Hospital Fire in Saint Lucia

This paper provides a field report on a hospital fire at the St. Jude hospital in the Eastern Caribbean Island of Saint Lucia. The hospital was completely destroyed by the fire and three deaths were recorded. This paper analyses the emergency response to this hospital fire and discusses the lessons learned from this experience. This is a valuable lesion for developing countries in the Caribbean, especially since there have been four hospital fires reported in the Caribbean within the past decade.

Beyond Tropical Storms: Understanding Disturbance and Forest Dynamics

Most of the knowledge of tropical storm effects on forests is from studies conducted in reserves and protected areas. Effects on other settings such as urban forests, coastal forests, and in landscapes with mixed land uses and covers are, comparatively, less studied. Yet research from a range of forest types and landscape compositions is needed to inform actions associated with management, mitigation, or restoration efforts before and after storms. Studies of forests comprising various conditions, along with long-term observations, would offer unique insights into the varied and cumulative effects of tropical storms on forest ecosystems. The Caribbean island of Puerto Rico is one of the sites where most tropical storm-forest studies are conducted, yet studies come from a limited set of sites. Using Puerto Rico as a case study, we emphasize the need for broader research approaches that represent the wider range of forests that are exposed to tropical storms. Such an approach will provide valuable knowledge and understanding needed to inform and take actions across landscape settings, forest context, and socio-environmental conditions.