scholarly journals Levels of different cytokines in women and men with asymptomatic genital infection caused by Chlamydia

2019 ◽  
Vol 13 (09) ◽  
pp. 847-850
Author(s):  
Alessandra Bua ◽  
Sara Cannas ◽  
Stefania Zanetti ◽  
Paola Molicotti
Keyword(s):  
Immune Response ◽  
Statistical Analysis ◽  
Chlamydia Trachomatis ◽  
Genital Infection ◽  
Healthy Controls ◽  
Rt Pcr ◽  
Chlamydia Trachomatis Infection ◽  
Molecular Assays ◽  
Significant Difference ◽  
Student Test

Introduction: Immune response to genital Chlamydia trachomatis infection is involved in both immunity and pathology. The cytokine profile during infection has been implicated in the disease outcome, either resolution or severe sequelae. Methodology: In total, 3900 patients were analyzed for presence of genital infections caused by Chlamydia using molecular assays. Interleukins (IL) IL-10, IL-17, IL-6, IL-2 and chemokine IP-10 were estimated by ELISA in urine, cervical swabs and semen samples. Statistical analysis was performed using the T student test. Results: A total of 47 out of 3900 samples (1.2%) were found to be positive for Chlamydia trachomatis based on the Real Time (RT) PCR results. Statistical analysis revealed that the differences between Chlamydia trachomatis positive and negative samples regarding levels of cytokines were not significant. Conclusions: Our results demonstrated that no significant difference in cytokine concentrations exists in Chlamydia trachomatis infected patients when compared to healthy controls. In further study, we aim to test on a greater number of positive samples a greater number of cytokines involved in the immune response to Chlamydia trachomatis infections.

scholarly journals Specific-Pathogen-Free Pigs as an Animal Model for Studying Chlamydia trachomatis Genital Infection

2005 ◽  
Vol 73 (12) ◽  
pp. 8317-8321 ◽  
Author(s):  
Daisy Vanrompay ◽  
Thi Q. T. Hoang ◽  
Liselotte De Vos ◽  
Kristel Verminnen ◽  
Taher Harkinezhad ◽  
...  
Keyword(s):  
Immune Response ◽  
Animal Model ◽  
Chlamydia Trachomatis ◽  
Immunoglobulin M ◽  
Large Animal Model ◽  
Genital Infection ◽  
Large Animal ◽  
Specific Pathogen Free ◽  
Humoral Immune ◽  
Specific Pathogen

ABSTRACT The purpose of the present study was to evaluate pigs as a large-animal model for female genital infection with two Chlamydia trachomatis human serovar E strains. Sixteen-week-old specific-pathogen-free female pigs (gilts) were intravaginally infected with the trachoma type E reference strain Bour or the urogenital serovar E strain 468. Several conclusions can be drawn from our findings on the pathogenicity of a primary C. trachomatis genital infection in gilts. First of all, we demonstrated that the serovar E strains Bour and 468 could ascend in the genital tract of gilts. The serovar E strains could replicate in the superficial columnar cervical epithelium and in the superficial epithelial layer of the uterus, which are known to be the specific target sites for a C. trachomatis genital infection in women. Second, inflammation and pathology occurred at the replication sites. Third, the organisms could trigger a humoral immune response, as demonstrated by the presence of immunoglobulin M (IgM), IgG, and IgA in both serum and genital secretion samples. Our findings imply that the pig model might be useful for studying the pathology, pathogenesis, and immune response to a C. trachomatis infection of the genital system.

scholarly journals Humoral immune response to plasmid protein pgp3 in patients with Chlamydia trachomatis infection.

1994 ◽  
Vol 62 (12) ◽  
pp. 5491-5497 ◽  
Author(s):  
M Comanducci ◽  
R Manetti ◽  
L Bini ◽  
A Santucci ◽  
V Pallini ◽  
...  
Keyword(s):  
Immune Response ◽  
Chlamydia Trachomatis ◽  
Humoral Immune Response ◽  
Chlamydia Trachomatis Infection ◽  
Humoral Immune

scholarly journals Cellular immune response during uncomplicated genital infection with Chlamydia trachomatis in humans.

1981 ◽  
Vol 34 (1) ◽  
pp. 98-104 ◽  
Author(s):  
R C Brunham ◽  
D H Martin ◽  
C C Kuo ◽  
S P Wang ◽  
C E Stevens ◽  
...  
Keyword(s):  
Immune Response ◽  
Chlamydia Trachomatis ◽  
Cellular Immune Response ◽  
Genital Infection ◽  
Cellular Immune

scholarly journals Comparison of genovars and Chlamydia trachomatis infection loads in ocular samples from children in two distinct cohorts in Sudan and Morocco

2021 ◽  
Vol 15 (8) ◽  
pp. e0009655
Author(s):  
Ehsan Ghasemian ◽  
Aleksandra Inic-Kanada ◽  
Astrid Collingro ◽  
Lamiss Mejdoubi ◽  
Hadeel Alchalabi ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Outer Membrane ◽  
Real Time ◽  
Bacterial Load ◽  
Protein A ◽  
Healthy Controls ◽  
Geographical Differences ◽  
Chlamydia Trachomatis Infection ◽  
Membrane Complex ◽  
Moroccan Patients

Trachoma is a blinding disease caused by repeated conjunctival infection with different Chlamydia trachomatis (Ct) genovars. Ct B genovars have been associated with more severe trachoma symptoms. Here, we investigated associations between Ct genovars and bacterial loads in ocular samples from two distinct geographical locations in Africa, which are currently unclear. We tested ocular swabs from 77 Moroccan children (28 with trachomatous inflammation-follicular (TF) and 49 healthy controls), and 96 Sudanese children (54 with TF and 42 healthy controls) with a Ct-specific real-time polymerase chain reaction (PCR) assay. To estimate bacterial loads, Ct-positive samples were further processed by multiplex real-time qPCR to amplify the chromosomal outer membrane complex B and plasmid open reading frame 2 of Ct. Genotyping was performed by PCR-based amplification of the outer membrane protein A gene (~1120 base pairs) of Ct and Sanger sequencing. Ct-positivities among the Moroccan and Sudanese patient groups were 60·7% and 31·5%, respectively. Significantly more Sudanese patients than Moroccan patients were genovar A-positive. In contrast, B genovars were significantly more prevalent in Moroccan patients than in Sudanese patients. Significantly higher Ct loads were found in samples positive for B genovars (598·596) than A genovar (51·005). Geographical differences contributed to the distributions of different ocular Ct genovars. B genovars may induce a higher bacterial load than A genovars in trachoma patients. Our findings emphasize the importance of conducting broader studies to elucidate if the noted difference in multiplication abilities are genovar and/or endemicity level dependent.

scholarly journals Chlamydia trachomatis screening in resource-limited countries – Comparison of diagnostic accuracy of 3 different assays

2018 ◽  
Vol 12 (09) ◽  
pp. 733-740
Author(s):  
Jelena Zivadin Tosic-Pajic ◽  
Predrag Sazdanovic ◽  
Marija Sorak ◽  
Jelena Cukic ◽  
Aleksandra Arsovic ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Diagnostic Accuracy ◽  
Statistical Significance ◽  
Acute Infection ◽  
False Negative ◽  
Screening Tests ◽  
Rt Pcr ◽  
Serum Igg ◽  
Significant Difference ◽  
Youden’S Index

Introduction: Commercially available assays were evaluated in order to determine diagnostic accuracy of Chlamydia trachomatis specific tests for screening. Methods: The study included 225 sexually active men and women, who were tested for genital chlamydial infection in Institute of Public Health Kragujevac. Three screening tests were used: direct immunofluorescence (DIF) and rapid lateral immunochromatographic test (RT) for qualitative detection of chlamydial antigens and immunoenzyme (ELISA) test for detection of serum levels of anti-chlamydial IgA and IgG antibodies. Diagnostic efficiency of these tests were determined in relation to results obtained by RT-PCR method. Results: Statistical significance between the results obtained by RT-PCR as a gold standard and DIF, RT and ELISA were analyzed using chi-square (χ2) test. Statistical analysis showed a significant difference between RT-PCR and analyzed screening tests: DIF (χ2 = 303; p < 0.001), RT (χ2 = 4.19; p = 0.041), serum IgA (χ2 = 4.19; p = 0.041) and serum IgG (χ2 = 67; p < 0.001) which indicates poor agreement between these tests. Large numbers of false positive (FP) and false negative (FN) results were observed for all tested assays. According to Youden’s index, serum IgG and DIF testing demonstrated the most-balanced sensitivity-specificity rate. RT assay exhibits the highest expanded Youden’s index, as well as the best overall diagnostic accuracy. Conclusions: None of evaluated screening tests can be recommended as individual method for the diagnosis of acute infection. We suppose that RT-PCR is unlikely to be a cost-effective screening strategy within the Serbian health system.

scholarly journals Induction of Protective Immunity against Chlamydia trachomatis Genital Infection by a Vaccine Based on Major Outer Membrane Protein–Lipophilic Immune Response-Stimulating Complexes

2000 ◽  
Vol 68 (12) ◽  
pp. 6798-6806 ◽  
Author(s):  
Joseph U. Igietseme ◽  
Andrew Murdin
Keyword(s):  
Immune Response ◽  
Chlamydia Trachomatis ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Major Outer Membrane Protein ◽  
Genital Infection ◽  
Th1 Cell ◽  
Th1 Response

ABSTRACT The significance of delivery systems in modern vaccine design strategies is underscored by the fact that a promising vaccine formulation may fail in vivo due to an inappropriate delivery method. We evaluated the immunogenicity and efficacy of a candidate vaccine comprising the major outer membrane protein (MOMP) of Chlamydia trachomatis delivered with the lipophilic immune response-stimulating complexes (ISCOMs) as a vehicle with adjuvant properties, in a murine model of chlamydial genital infection. Immunocompetent BALB/c mice were immunized intranasally (IN) or intramuscularly (IM) with MOMP, MOMP-ISCOMs, and live or heat-inactivated C. trachomatis serovar D. The level of local genital mucosal Th1 response was measured by assaying for antigen-specific Th1 cell induction and recruitment into the genital mucosa at different times after immunization. Immunization with MOMP-ISCOMs by the IM route induced the greatest and fastest local genital mucosal Th1 response, first detectable 2 weeks after exposure. Among the other routes and regimens tested, only IN immunization with MOMP-ISCOMs induced detectable and statistically significant levels of local genital mucosal Th1 response during the 8-week test period (P < 0.001). In addition, when T cells from immunized mice were adoptively transferred into syngeneic naive animals and challenged intravaginally with Chlamydia, recipients of IM immunization of MOMP-ISCOMs cleared their infection within 1 week and were resistant to reinfection. Animals that received IN immunization of MOMP-ISCOMs were partially protected, shedding fewer chlamydiae than did control mice. Altogether, the results suggested that IM delivery of MOMP-ISCOMs may be a suitable vaccine regimen potentially capable of inducing protective mucosal immunity against C. trachomatisinfection.

scholarly journals Association of Genital Chlamydia trachomatis Infection with Female Infer-tility, Study in a Tertiary Care Hospital in Eastern India

2015 ◽  
Vol 9 (1) ◽  
pp. 110-116 ◽  
Author(s):  
Mallika Ghosh ◽  
Subhadip Choudhuri ◽  
Reena Ghosh Ray ◽  
Basudev Bhattacharya ◽  
Sujata Bhattacharya
Keyword(s):  
Chlamydia Trachomatis ◽  
Tertiary Care ◽  
Elisa Test ◽  
Care Hospital ◽  
Serological Tests ◽  
Chlamydia Trachomatis Infection ◽  
Sexually Transmitted ◽  
Medical College ◽  
Significant Difference ◽  
Iga Antibody

Background:Chlamydia trachomatisis recognized as one of the most common sexually transmitted pathogen in the world. 50-80% of infected females are asymptomatic. These untreated women are at risk of developing chronic sequelae leading to tubal pathology causing infertility. Infertility is defined as 1 year of unprotected intercourse without pregnancy. It may be primary or secondary.Aim:To find out the association of genitalChlamydia trachomatisinfection with female infertility.Materials and Methodology:This case control study has been carried out in collaboration with R. G. Kar Medical College and Institute of Post Graduate Medical Education &amp; Research, India, between July 2012 and June 2013. 40 infertile and 40 pregnant women were enrolled by purposive sampling as per inclusion and exclusion criteria. ELISA test was performed to detect serum IgG and IgA antibody against recombinant analogs of MOMP and 3 different PCR assays were done targeting MOMP and rRNA DNA from DNA extracted from first void urine.Results:IgG seropositivity was significantly higher (15%vs0%,P=.0255) in cases than controls, though there was no significant difference in the proportion of IgA seropositivity among 2 groups (12.5%vs2.5%,P=0.2007). Out of 80 samples 2 samples showed the production of amplicons with R1 – R2 primers. Only 1 sample gave positive result with production of amplicons with all the 3 primers used (R1 – R2, CT0005 – CT06 and JM15 – JM16).Conclusion:PersistentC. trachomatisinfection must be recognized as a risk factor of infertility in this region of India. The low PCR positivity in FVU sample helps to conclude the diagnostic utility of serological tests in screening of infertile women.

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