scholarly journals Effect of bacterial probiotics bio encapsulated into Artemia franciscana on weight and length of the shortfin silverside (Chirostoma humboldtianum), and PCR DGGE characterization of its intestinal bacterial community

2017 ◽  
Vol 45 (5) ◽  
pp. 1031-1043 ◽  
Author(s):  
Gabriela Vazquez Silva ◽  
Hugo Ramirez Saad ◽  
Jose Aguirre Garrido ◽  
Lino Mayorga Reyes ◽  
Alejandro Azaola Espinosa ◽  
...  
2020 ◽  
Vol 13 (11) ◽  
pp. 1
Author(s):  
A. R. B. Zanco ◽  
A. Ferreira ◽  
G. C. M. Berber ◽  
E. N. Gonzaga ◽  
D. C. C. Sabino

The different integrated production systems can directly interfere with its bacterial community. The present study aimed to assess density, bacterial diversity and the influence of dry and rainy season in different integrated and an exclusive production system. The fallow and a native forest area was assessed to. Samples were collected in 2012 March and September. The isolation were carried out into Petri dishes containing DYGS medium. The number of colony forming units (CFU) was counted after 48 hours and. The bacterial density ranged between 106 and 107 CFU g-1 soil. The crop system affected the dynamics of the bacterial community only in the rainy season. The rainy season showed greater density of total bacteria when compared to the dry period regardless of the cropping system. The dendrograms with 80 % similarity showed thirteen and fourteen groups in the rainy and dry seasons. Isolates with the capacity to solubilize phosphate in vitro were obtained from all areas in the two seasons, but this feature has been prevalent in bacteria isolated during the rainy season


2021 ◽  
Author(s):  
Yurong Wang ◽  
Fanshu Xiang ◽  
Zhendong Zhang ◽  
Qiangchuan Hou ◽  
Zhuang Guo

Chemosphere ◽  
2021 ◽  
pp. 133168
Author(s):  
Balakrishnan Muthukumar ◽  
Punniyakotti Parthipan ◽  
Mohamad S. AlSalhi ◽  
Nataraj Srinivasa Prabhu ◽  
T. Nageswara Rao ◽  
...  

2007 ◽  
Vol 292 (5) ◽  
pp. R2039-R2047 ◽  
Author(s):  
Michael A. Menze ◽  
Steven C. Hand

Evaluation of apoptotic processes downstream of the mitochondrion reveals caspase-9- and low levels of caspase-3-like activities in partly purified extracts of Artemia franciscana embryos. However, in contrast to experiments with extracts of human hepatoma cells, cytochrome c fails to activate caspase-3 or -9 in extracts from A. franciscana. Furthermore, caspase-9 activity is sensitive to exogenous calcium. The addition of 5 mM calcium leads to a 4.86 ± 0.19 fold (SD) ( n = 3) increase in activity, which is fully prevented with 150 mM KCl. As with mammalian systems, high ATP (>1.25 mM) suppresses caspase activity in A. franciscana extracts. A strong inhibition of caspase-9 activity was also found by GTP. Comparison of GTP-induced inhibition of caspase-9 at 0 and 2.5 mM MgCl2 indicates that free (nonchelated) GTP is likely to be the inhibitory form. The strongest inhibition among all nucleotides tested was with ADP. Inhibition by ADP in the presence of Mg2+ is 60-fold greater in diapause embryos than in postdiapause embryos. Because ADP does not change appreciably in concentration between the two physiological states, it is likely that this differential sensitivity to Mg2+-ADP is important in avoiding caspase activation during diapause. Finally, mixtures of nucleotides that mimic physiological concentrations in postdiapause and diapause states underscore the depressive action of these regulators on caspase-9 during diapause. Our biochemical characterization of caspase-like activity in A. franciscana extracts reveals that multiple mechanisms are in place to reduce the probability of apoptosis under conditions of energy limitation in this embryo.


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