Density and morphological characterization of bacteria in integrated and exclusive production systems

2020 ◽  
Vol 13 (11) ◽  
pp. 1
Author(s):  
A. R. B. Zanco ◽  
A. Ferreira ◽  
G. C. M. Berber ◽  
E. N. Gonzaga ◽  
D. C. C. Sabino
Keyword(s):  
Bacterial Community ◽  
Rainy Season ◽  
Production Systems ◽  
Cropping System ◽  
Morphological Characterization ◽  
Native Forest ◽  
Exclusive Production ◽  
Colony Forming Units

The different integrated production systems can directly interfere with its bacterial community. The present study aimed to assess density, bacterial diversity and the influence of dry and rainy season in different integrated and an exclusive production system. The fallow and a native forest area was assessed to. Samples were collected in 2012 March and September. The isolation were carried out into Petri dishes containing DYGS medium. The number of colony forming units (CFU) was counted after 48 hours and. The bacterial density ranged between 106 and 107 CFU g-1 soil. The crop system affected the dynamics of the bacterial community only in the rainy season. The rainy season showed greater density of total bacteria when compared to the dry period regardless of the cropping system. The dendrograms with 80 % similarity showed thirteen and fourteen groups in the rainy and dry seasons. Isolates with the capacity to solubilize phosphate in vitro were obtained from all areas in the two seasons, but this feature has been prevalent in bacteria isolated during the rainy season

scholarly journals Density and morphological characterization of bacteria in integrated and exclusive production systems

2020 ◽  
Vol 13 (11) ◽  
pp. 1
Author(s):  
A. R. B. Zanco ◽  
A. Ferreira ◽  
G. C. M. Berber ◽  
E. N. Gonzaga ◽  
D. C. C. Sabino
Keyword(s):  
Bacterial Community ◽  
Rainy Season ◽  
Production Systems ◽  
Cropping System ◽  
Morphological Characterization ◽  
Native Forest ◽  
Exclusive Production ◽  
Colony Forming Units

The different integrated production systems can directly interfere with its bacterial community. The present study aimed to assess density, bacterial diversity and the influence of dry and rainy season in different integrated and an exclusive production system. The fallow and a native forest area was assessed to. Samples were collected in 2012 March and September. The isolation were carried out into Petri dishes containing DYGS medium. The number of colony forming units (CFU) was counted after 48 hours and. The bacterial density ranged between 106 and 107 CFU g-1 soil. The crop system affected the dynamics of the bacterial community only in the rainy season. The rainy season showed greater density of total bacteria when compared to the dry period regardless of the cropping system. The dendrograms with 80 % similarity showed thirteen and fourteen groups in the rainy and dry seasons. Isolates with the capacity to solubilize phosphate in vitro were obtained from all areas in the two seasons, but this feature has been prevalent in bacteria isolated during the rainy season.

scholarly journals BIODIVERSIDADE DE BACTÉRIAS ANTAGÔNICAS A FUNGOS ASSOCIADOS À SINDROME DA MORTE DO CAPIM-MARANDU (Brachiaria brizantha)

Nativa ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 454-459
Author(s):  
Maira Laís Both Bourscheidt ◽  
Kellen Banhos do Carmo ◽  
Bruno Carneiro Pedreira ◽  
Gilcele de Campos Martin Berber ◽  
Anderson Ferreira
Keyword(s):  
Biological Control ◽  
Rainy Season ◽  
Production Systems ◽  
Dry Season ◽  
Native Forest ◽  
Bacterial Isolates ◽  
Fusarium Spp ◽  
Brachiaria Brizantha

A síndrome da morte do capim-marandu (Brachiaria brizantha) é um dos principais problemas na degradação das pastagens no bioma Amazônia. A morte das forrageiras ocorre durante a época chuvosa por suscetibilidade a fitopatógenos. Assim, objetivou-se estudar sistemas de produção e selecionar bactérias antagônicas a Fusarium spp., um dos agentes fitopatogênicos. Os isolados bacterianos foram obtidos de 10 sistemas de produção, mata nativa e pousio, na chuva e na seca. Realizou-se a caracterização molecular de isolados por sequenciamento. Dois isolados de Fusarium foram testados. Para o patógeno 1, na época da chuva, 7,5% dos isolados apresentaram antagonismo enquanto na seca foi 15%. Para o patógeno 2, nem todos os isolados positivos para o patógeno 1 foram eficientes no controle, demonstrando a ocorrência de interações entre isolados bacterianos e Fusarium spp. Na chuva, para o patógeno 1, a mata apresentou maior número de isolados positivos (20%). Na seca, os tratamentos mata, 1-Floresta e 8-integração lavoura floresta para o patógeno 1, foram os que apresentaram maior número de positivos (25%). Na identificação molecular, 8 foram os gêneros bacterianos encontrados: Burkholderia, Bacillus, Brevibacillus, Streptomyces, Pseudomonas, Escherichia, Paenibacillus e Pandoraea, em que a maioria é descrita como capaz de controlar Fusarium spp. in vitro. Palavras-chave: Burkholderia; controle biológico; integração lavoura-pecuária-floresta.   Biodiversity of bacteria antagonic to fungi associated with syndrome's death palisadegrass (Brachiaria brizantha)   ABSTRACT: The death syndrome of marandu grass (Brachiaria brizantha) is one of the main problems in the degradation of pastures in the Amazon biome. Plant death occurs during the rainy season due to susceptibility to phytopathogens. Thus, the objective was to study production systems and select bacteria antagonistic to Fusarium spp., one of the phytopathogenic agents. Bacterial isolates were obtained from 10 production systems, native forest and fallow, in rain and drought season. Molecular characterization of isolates was carried out by sequencing. Two Fusarium isolates were assessed. For pathogen 1, in the rainy season, 7.5% of the isolates showed antagonism, while in the dry season it was 15%. For pathogen 2, not all isolates positive for pathogen 1 were efficient in the control, showing correlations between bacterial isolates and Fusarium spp. In the rain, for pathogen 1, the forest showed the highest number of positive isolates (20%). In the dry season, the treatments forest, 1-Forest and 8-plant-forest integration for pathogen 1, were ones with the highest number of positives (25%). In the molecular identification, 8 bacterial genera were found: Burkholderia, Bacillus, Brevibacillus, Streptomyces, Pseudomonas, Escherichia, Paenibacillus and Pandoraea, most of which are described as able to controlling Fusarium spp. in vitro. Keywords: Burkholderia; biological control; integrated crop-livestock-forest.

scholarly journals Fabrication and Characterization of Scaffolds of Poly(ε-caprolactone)/Biosilicate® Biocomposites Prepared by Generative Manufacturing Process

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Daniel Aparecido Lopes Vieira da Cunha ◽  
Paulo Inforçatti Neto ◽  
Kelli Cristina Micocci ◽  
Caroline Faria Bellani ◽  
Heloisa Sobreiro Selistre-de-Araujo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Manufacturing Process ◽  
Potential Application ◽  
Morphological Characterization ◽  
Mechanical Compression ◽  
Compression Tests ◽  
Extrusion Head ◽  
Fabrication And Characterization

Scaffolds of poly(ε-caprolactone) (PCL) and their biocomposites with 0, 1, 3, and 5 wt.% Biosilicate® were fabricated by the generative manufacturing process coupled with a vertical miniscrew extrusion head to application for restoration of bone tissue. Their morphological characterization indicated the designed 0°/90° architecture range of pore sizes and their interconnectivity is feasible for tissue engineering applications. Mechanical compression tests revealed an up to 57% increase in the stiffness of the scaffold structures with the addition of 1 to 5 wt.% Biosilicate® to the biocomposite. No toxicity was detected in the scaffolds tested by in vitro cell viability with MC3T3-E1 preosteoblast cell line. The results highlighted the potential application of scaffolds fabricated with poly(ε-caprolactone)/Biosilicate® to tissue engineering.

scholarly journals Morphological characterization of pre- and peri-implantation in vitro cultured, somatic cell nuclear transfer and in vivo derived ovine embryos

Reproduction ◽  
2005 ◽  
Vol 130 (5) ◽  
pp. 681-694 ◽  
Author(s):  
P Tveden-Nyborg ◽  
T T Peura ◽  
K M Hartwich ◽  
S K Walker ◽  
P Maddox-Hyttel
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Morphological Characterization ◽  
Morphological Development ◽  
Embryonic Disc ◽  
Transmission Electron

The processes of cellular differentiation were studied in somatic cell nuclear transfer (SCNT), in vitro cultured (IVC) and in vivo developed (in vivo) ovine embryos on days 7, 9, 11, 13, 17 and 19. SCNT embryos were constructed from in vitro matured oocytes and granulosa cells, and IVC embryos were produced by in vitro culture of in vivo fertilized zygotes. Most SCNT and IVC embryos were transferred to recipients on day 6 while some remained in culture for day 7 processing. In vivo embryos were collected as zygotes, transferred to intermediate recipients and retransferred to final recipients on day 6. All embryos were processed for examination by light and transmission electron microscopy or immunohistochemical labelling for alpha-1-fetoprotein and vimentin. Overall, morphological development of in vivo embryos was superior to IVC and SCNT embryos. Day 7 and particularly day 9 IVC and SCNT embryos had impaired hypoblast development, some lacking identifiable inner cell masses. On day 11, only in vivo and IVC embryos had developed an embryonic disc, and gastrulation was evident in half of in vivo embryos and one IVC embryo. By day 13, all in vivo embryos had completed gastrulation whereas IVC and SCNT embryos remained retarded. On days 17 and 19, in vivo embryos had significantly more somites and a more developed allantois than IVC and SCNT embryos. We conclude that IVC and particularly SCNT procedures cause a retardation of embryo development and cell differentiation at days 7–19 of gestation.

scholarly journals IN VIVO AND IN VITRO STUDIES ON MORPHOLOGICAL CHARACTERIZATION OF SEPTORIA LYCOPERSICI CAUSING LEAF SPOT DISEASE IN TOMATO

2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
Aabid . ◽  
Sabiha Ashraf ◽  
Hilal A. Malik ◽  
Rakshanda Zargar ◽  
Shaheena A. Nagoo ◽  
...  
Keyword(s):  
Leaf Spot ◽  
Morphological Characterization ◽  
Leaf Spot Disease ◽  
Potato Dextrose Agar Medium ◽  
Spot Disease ◽  
Spore Mass ◽  
Septoria Lycopersici

Septoria lycopersici responsible for Septoria leaf spot disease was observed on the leaves of tomato. Septoria lycopersici was isolated and completion of Koch’s postulates confirmed that the fungus was causal agent of the leaf spot disease. The fungus was cultured on potato dextrose agar medium. The fungus was very slow growing with 8-12 mm radial growth as recorded after 30 days of incubation. The fungus produced off white, irregular, hardened blackish mycelial growth oozing spore mass from pycnidia. Pycnidia were dark brown to black, globose to sub globose, ostiolated and thick walled. Pycnidiospores were filiform, straight with pointed to rounded ends.

In vitro morphological characterization of the bursal reticuloepithelial (REp) cells of chicken

1990 ◽  
Vol 46 (10) ◽  
pp. 1060-1063 ◽  
Author(s):  
F. Giannessi ◽  
F. Bianchi ◽  
A. Dolfi ◽  
M. Lupetti ◽  
S. Michelucci
Keyword(s):  
Morphological Characterization

scholarly journals Distribution and Characterization of Monilinia spp. Causing Apple Fruit Decay in Serbia

Plant Disease ◽  
2018 ◽  
Vol 102 (2) ◽  
pp. 359-369 ◽  
Author(s):  
Miljan Vasić ◽  
Ivana Vico ◽  
Wayne M. Jurick ◽  
Nataša Duduk
Keyword(s):  
Pathogen Detection ◽  
Management Strategies ◽  
Brown Rot ◽  
Morphological Characterization ◽  
Apple Fruit ◽  
Postharvest Disease ◽  
Pome Fruit

Brown rot, caused by Monilinia spp., is an economically important pre- and postharvest disease of pome and stone fruits worldwide. In Serbia, apple is the most widely grown pome fruit, and the distribution of economically important Monilinia spp. responsible for apple brown rot is unknown. Hence, we conducted a three year survey, from 2010 to 2012, where 349 isolates were obtained from six orchards and four storage facilities from five different apple cultivars with brown rot symptoms. Morphological characterization of the isolates, multiplex PCR, and phylogenetic analysis revealed four species: M. fructigena, M. laxa, M. fructicola, and Monilia polystroma. All species were found in the orchard and in storage, with M. fructigena predominating, followed by M. polystroma. Representative isolates were analyzed in vitro and in vivo where differences in growth rate, sporulation, and virulence on apple fruit were observed. Findings from this investigation demonstrate diversity in the species responsible for pre- and postharvest apple brown rot, which has significant implications for pathogen detection and for developing disease-specific management strategies.

Sign in / Sign up

Export Citation Format

Share Document