scholarly journals Fibrosis in Chronic Hepatitis C: Correlation between Immunohistochemically-Assessed Virus Load with Steatosis and Cellular Iron Content

2016 ◽  
Vol 4 (4) ◽  
pp. 578-584
Author(s):  
Maha Akl ◽  
Ali EL Hindawi ◽  
Maha Mosaad ◽  
Ahmed Montasser ◽  
Ahmed El Ray ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Viral Load ◽  
Hepatitis C ◽  
Chronic Hepatitis C ◽  
Liver Damage ◽  
Cellular Iron ◽  
Iron Load ◽  
Cirrhotic Group ◽  
Sirius Red

AIM: We aimed study impact of hepatocytic viral load, steatosis, and iron load on fibrosis in chronic hepatitis C and role of VEGF and VEGFR overexpression in cirrhotic cases in evolving HCC.MATERIAL AND METHODS: Total of 120 cases were included from TBRI and Beaujon Hospital as chronic hepatitis C (CHC), post-hepatitis C cirrhosis, and HCC. Cases of CHC were stained for Sirius red, Prussian blue and immunohistochemically (IHC) for HCV-NS3/NS4. HCC were stained IHC for VEGF and by FISH.RESULTS: Stage of fibrosis was significantly correlated with inflammation in CHC (P < 0.01). Noticed iron load did not correlate with fibrosis. Steatosis was associated with higher inflammation and fibrosis. The cellular viral load did not correlate with inflammation, steatosis or fibrosis. VEGF by IHC was significantly higher in cases of HCC when compared to cirrhotic group (P < 0.001). Amplification of VEGFR2 was confirmed in 40% of cases of HCC. Scoring of VEGF by IHC was the good indicator  of VEGFR2 amplification by FISH (P < 0.005).CONCLUSION: Grade of inflammation is the factor affecting fibrosis in CHC. The degree of liver damage is not related to cellular viral load or iron load. Steatosis is associated with higher inflammation and fibrosis. VEGF by IHC is correlated with overexpression of VEGFR2 by FISH.

scholarly journals Role of chemokines and their receptors in viral persistence and liver damage during chronic hepatitis C virus infection

2008 ◽  
Vol 14 (47) ◽  
pp. 7149 ◽  
Author(s):  
Juan R Larrubia ◽  
Selma Benito-Martínez ◽  
Miryam Calvino ◽  
Eduardo Sanz-de-Villalobos ◽  
Trinidad Parra-Cid
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Chronic Hepatitis C ◽  
Liver Damage ◽  
Viral Persistence ◽  
Hepatitis C Virus Infection ◽  
Chronic Hepatitis C Virus

Profiles of HCV core protein and viremia in chronic Hepatitis C: possible protective role of core antigen in liver damage

2005 ◽  
Vol 76 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Alessandro Carabaich ◽  
Mariagrazia Ruvoletto ◽  
Elisabetta Bernardinello ◽  
Natascia Tono ◽  
Luisa Cavalletto ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis C ◽  
Chronic Hepatitis C ◽  
Liver Damage ◽  
Core Protein ◽  
Protective Role ◽  
Core Antigen ◽  
Hcv Core Protein

Stat-1 and IRF-1: two factors in the signaling pathway of interferons related to viral load and liver damage in chronic hepatitis C (CH-C)

2002 ◽  
Vol 36 ◽  
pp. 225
Author(s):  
Elvira Molano ◽  
Esther Larrea ◽  
Patricia Boya ◽  
Elena Baixeras ◽  
Iosu Sola ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Viral Load ◽  
Hepatitis C ◽  
Signaling Pathway ◽  
Chronic Hepatitis C ◽  
Liver Damage ◽  
Two Factors

scholarly journals Intrahepatic TLR3 and IFNL3 Expressions Are Associated with Stages of Fibrosis in Chronic Hepatitis C

Viruses ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1103
Author(s):  
Keyla Santos Guedes de Sá ◽  
Ednelza da Silva Graça Amoras ◽  
Simone Regina Souza da Silva Conde ◽  
Maria Alice Freitas Queiroz ◽  
Izaura Maria Vieira Cayres-Vallinoto ◽  
...  
Keyword(s):  
Immune Response ◽  
Chronic Hepatitis ◽  
Viral Load ◽  
Hepatitis C ◽  
Chronic Hepatitis C ◽  
Control Group ◽  
Gene Expressions ◽  
Healthy Control ◽  
Advanced Stages ◽  
Chronic Hcv

An inefficient immune response against the hepatitis C virus (HCV), combined with viral evasion mechanisms, is responsible for the chronicity of infection. The need to evaluate the innate mechanisms of the immune response, such as TLR3 and IFN-λ3, and their relationship with the virus–host interaction is important for understanding the pathogenesis of chronic hepatitis C. The present study aimed to investigate the gene expressions of TRL3 and IFNL3 in liver tissue, seeking to evaluate whether these could be potential biomarkers of HCV infection. A total of 23 liver biopsy samples were collected from patients with chronic HCV, and 8 biopsies were collected from healthy control patients. RNA extraction, reverse transcription and qPCR were performed to quantify the relative gene expressions of TLR3 and IFNL3. Data on the viral load; AST, ALT, GGT and AFP levels; and the viral genotype were collected from the patients′ medical records. The intrahepatic expression of TLR3 (p = 0.0326) was higher in chronic HCV carriers than in the control group, and the expression of IFNL3 (p = 0.0037) was lower in chronic HCV carriers than in the healthy control group. The expression levels of TLR3 (p = 0.0030) and IFNL3 (p = 0.0036) were higher in the early stages of fibrosis and of necroinflammatory activity in the liver; in contrast, TLR3 and IFNL3 expressions were lower in the more advanced stages of fibrosis and inflammation. There was no correlation between the gene expression and the serum viral load. Regarding the initial METAVIR scale scores, liver transaminase levels were lower in patients with advanced fibrosis when correlated with TLR3 and IFNL3 gene expressions. The results suggest that in the early stages of the development of hepatic fibrosis, TLR3 and IFN-λ3 play important roles in the antiviral response and in the modulation of the tolerogenic liver environment because there is a decrease in the intrahepatic expressions of TLR3 and IFNL3 in the advanced stages of fibrosis, probably due to viral evasion mechanisms.

Association of insulin resistance, viral load, and adipokine levels with liver histology in patients with chronic hepatitis C

2012 ◽  
Vol 24 (12) ◽  
pp. 1393-1399 ◽  
Author(s):  
Hasan S. Zeki Aksu ◽  
Behice Kurtaran ◽  
Yusuf Onlen ◽  
Mustafa Namiduru ◽  
Ahmet C. Inkaya ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Chronic Hepatitis ◽  
Viral Load ◽  
Hepatitis C ◽  
Chronic Hepatitis C ◽  
Liver Histology
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