Assessment of serum levels of anti‑cyclic citrullinated peptide antibodies in patients with psoriatic arthritis: A cross‑sectional study in a Brazilian cohort

The Diagnostic Value of Anti-Cyclic Citrullinated Peptide Antibodies (anti-CCP) in Patients with Rheumatoid ArthritisA cross-sectional study was carried out to analyze the prevalence of anti-CCP antibodies and IgM RF in the sera of 160 randomly selected patients from the Rheumatology Department: 60 with RA, 50 with other rheumatic diseases (non-RA), 50 healthy controls (HC). The mean age of the group was 50.06+/-11.9 years. There were 141 females (88.1%) and 19 males (11.9%). RA patients fulfilled the revised ACR criteria. The mean duration of the disease was 82.4 months. Anti-CCP ELISA kit and IgM RF Latex test were used. The mean anti-CCP values were as follows: RA 60.4+/-57.6, non-RA 2.1+/-3.6, HC 1.3 +/-0.4 U/mL. Respectively, the mean values of IgM RF were: RA 515.8+/-525, non-RA 102+/-294, HC 15+/-57.5. Forty out of 60 (66.6%) RA patients were anti-CCP positive. Forty one out of 60 (68.3%) RA patients were positive for IgM RF. As expected, anti-CCP showed comparable sensitivity (66.8% vs. 68.3%) and higher specificity (98% vs. 87%) than IgM RF, at optimal cut-off values. The presence of either anti-CCP or IgM RF increased the testing sensitivity for the diagnosis of RA to 76.6%. AUC was greater for anti-CCP than for IgM RF (0.92 vs. 0.82).

Download Full-text

Synovial fluid and serum levels of IL-17, IL-23, and CCL-20 in rheumatoid arthritis and psoriatic arthritis: a Tunisian cross-sectional study

Rheumatology International ◽

10.1007/s00296-011-2231-1 ◽

2011 ◽

Vol 33 (1) ◽

pp. 265-266 ◽

Cited By ~ 13

Author(s):

Dalila Mrabet ◽

Lilia Laadhar ◽

Héla Sahli ◽

Béchir Zouari ◽

Slim Haouet ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Psoriatic Arthritis ◽

Synovial Fluid ◽

Serum Levels ◽

Cross Sectional Study ◽

Sectional Study ◽

Cross Sectional

Download Full-text

Relation of rheumatoid factor and anti-cyclic citrullinated peptide antibody with disease activity in rheumatoid arthritis: cross-sectional study

Rheumatology International ◽

10.1007/s00296-013-2734-z ◽

2013 ◽

Vol 33 (9) ◽

pp. 2373-2379 ◽

Cited By ~ 7

Author(s):

Jung-Yoon Choe ◽

Jisuk Bae ◽

Hwajeong Lee ◽

Sang-Cheol Bae ◽

Seong-Kyu Kim

Keyword(s):

Rheumatoid Arthritis ◽

Disease Activity ◽

Rheumatoid Factor ◽

Cross Sectional Study ◽

Sectional Study ◽

Cross Sectional ◽

Cyclic Citrullinated Peptide ◽

Peptide Antibody ◽

Citrullinated Peptide

Download Full-text

Rheumatoid Factor, Anti-cyclic Citrullinated Peptide and HLA-B Locus Antigens in Psoriatic Arthritis Patients in Bangladesh

Journal of Shaheed Suhrawardy Medical College ◽

10.3329/jssmc.v12i2.56892 ◽

2022 ◽

Vol 12 (2) ◽

pp. 109-114

Author(s):

Devolina Bhowmik ◽

Manash Chandra Sarker ◽

Shirin Tarafder ◽

Hosne Jahan ◽

Mst Naznin Tarana ◽

...

Keyword(s):

Psoriatic Arthritis ◽

Rheumatoid Factor ◽

Human Leukocyte ◽

Cross Sectional Study ◽

P Value ◽

Serum Samples ◽

Cross Sectional ◽

Cyclic Citrullinated Peptide ◽

B Locus ◽

Citrullinated Peptide

Introduction: Psoriatic arthritis (PsA) is established as a multifactorial disease resulting from a complex interplay between genetic, environmental and immunological factors. It is a seronegative arthritis but rheumatoid factor may be present in up to 15% of PsA patients Antibodies recognizing a cyclic citrullinated peptide are highly speciûc for rheumatoid arthritis (RA) but their role in PsA remains unclear. An increased prevalence of anti-CCP antibody in PsA is also reported. Study shows that HLA-DRB1 shared epitope is signiûcantly associated with the presence of anti-CCP antibody in PsA patients but this type of association is not found with other human leukocyte antigens. Objectives: The aim of this study was to investigate the frequency of anti-CCP and RF in PsA patients and their associations with HLA-B locus antigens. Methods: In this cross sectional study, we selected 50 unrelated consecutive patients with PsA according to CASPAR criteria for PsA. 6 ml of blood was collected from each patient for HLAB locus typing, RA test and test for anti-CCP. Patient’s serum samples were tested for RF by Nephelometric system and tests for anti-CCP were done by ELISA. HLA-B locus typing was done by PCR with sequence specific primer. Results: Among 50 PsA patients, 27 (54%) are female and 23 (46%) are male. RA test is positive in 10 (20%) patients and anti-CCP is positive in 7 (14%) patients. Significant association was found between HLA-B*37 and RF (p value= < 0.001). Conclusion: RF is present in 10 (20%) and anti-CCP is present in 7 (14%) PsA patients. HLAB* 37 was significantly found in RF positive patients. J Shaheed Suhrawardy Med Coll 2020; 12(2): 109-114

Download Full-text

Immuno-biological Assessments of Temporomandibular Joint Disease in Patients with Immune-mediated Rheumatic Conditions. A cross sectional study of 273 cases

Revista de Chimie ◽

10.37358/rc.17.12.6023 ◽

2018 ◽

Vol 68 (12) ◽

pp. 2987-2991

Author(s):

Cristina Iordache ◽

Bogdan Vascu ◽

Eugen Ancuta ◽

Rodica Chirieac ◽

Cristina Pomirleanu ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Psoriatic Arthritis ◽

Temporomandibular Joint ◽

Final Analysis ◽

Cross Sectional Study ◽

Joint Disease ◽

Sectional Study ◽

Cross Sectional ◽

Immune Parameters ◽

Immune Mediated

Temporomandibular joint (TMJ) is commonly involved in various immune-mediated rheumatic disorders accounting for significant disability and impaired quality of life. The aim of our study was to assess inflammatory and immune parameters in patients with TMJ arthritis related to rheumatoid arthritis (RA), juvenile idiopathic arthritis (JIA), ankylosing spondylitis (AS) and psoriatic arthritis (PsA) and to identify potential relation with severity and dysfunction of TMJ pathology. We performed a cross-sectional study in a cohort of 433 consecutive RA, 32 JIA, 258 AS, and 103 PsA. Only patients presenting with clinically significant TMJ involvement (273) related to their rheumatic condition were included in the final analysis. TMJ involvement is traditionally described in chronic inflammatory rheumatic disorders, particularly in patients with higher levels of inflammation as detected in rheumatoid arthritis and psoriatic arthritis. Disease activity and severity, as well as biological and positive serological assessments (rheumatoid factor, anti-cyclic citrullinated peptide, IL-1) remain significant determinants of the severity of TMJ arthritis.

Download Full-text

Evaluation of the Blood Level of Adiponectin in Pregnant Adolescents

Revista Brasileira de Ginecologia e Obstetrícia / RBGO Gynecology and Obstetrics ◽

10.1055/s-0041-1730288 ◽

2021 ◽

Vol 43 (06) ◽

pp. 429-435

Author(s):

Elaine Cristina Rocha Pádua ◽

Silvia Daher ◽

Isa de Pádua Cintra Sampaio ◽

Edward Araujo Júnior ◽

Cristina Falbo Guazzelli

Keyword(s):

Serum Levels ◽

Cross Sectional Study ◽

Serum Adiponectin ◽

Enzyme Linked Immunosorbent Assay ◽

Sectional Study ◽

Pregnant Adolescents ◽

Cross Sectional ◽

Adolescent Group ◽

Gestational Weight ◽

Intergroup Comparison

Abstract Objective To evaluate serum levels of adiponectin in pregnant adolescents between 30 and 36 weeks of gestation. Method: A prospective cross-sectional study enrolled 67 normal pregnant women between 30 and 36 weeks of gestation and eutrophic (body mass index [BMI]: 18.5–25 kg/m2), of which 36 were adolescents (< 20 years old) and 31 adults (≥ 20 years old). Serum adiponectin levels were determined by enzyme-linked immunosorbent assay (ELISA). The t-student or Mann-Whitney tests were used for intergroup comparison. Results Pregnant adolescents showed significantly higher serum adiponectin concentrations compared with pregnant adults (p = 0.04). No differences were observed in adiponectin levels in younger pregnant adolescents (< 16 years old) compared with older pregnant adolescents (≥ 16 years old). Adiponectin values were divided into 3 subgroups: < 3,000 ng/mL, between 3,000 and 5,000 ng/mL, and > 5,000 ng/mL. Birthweight was significantly higher in women > 5,000 ng/mL when compared with < 3,000 ng/mL in the adolescent group. No association between pregestational adiponectin levels and BMI, gestational weight gain, and gestational age was observed; however, there was a positive relation with birthweight (p = 0.0239). Conclusion Serum adiponectin values in pregnant adolescents between 30 and 36 weeks of gestation were higher compared with pregnant adults; however, no differences between younger and older pregnant adolescents were observed.

Download Full-text