scholarly journals Acer okamotoanum inhibits adipocyte differentiation by the�regulation of adipogenesis and lipolysis in 3T3‑L1 cells

2019 ◽  
Author(s):  
Ji Kim ◽  
Sanghyun Lee ◽  
Hyun Kim ◽  
Eun Cho
Keyword(s):  
Adipocyte Differentiation ◽  
Acer Okamotoanum

scholarly journals Flavonoids from Acer okamotoanum Inhibit Adipocyte Differentiation and Promote Lipolysis in the 3T3-L1 Cells

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1920 ◽  
Author(s):  
Ji Hyun Kim ◽  
Sanghyun Lee ◽  
Eun Ju Cho
Keyword(s):  
Fatty Acid Synthase ◽  
Glucose Transporter ◽  
Adipocyte Differentiation ◽  
Adenosine Monophosphate ◽  
Hormone Sensitive Lipase ◽  
Control Group ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor ◽  
Acer Okamotoanum ◽  
Related Proteins

Flavonoids, quercitrin, isoquercitrin (IQ), and afzelin, were isolated from ethyl acetate fraction of Acer okamotoanum. We investigated anti-obesity effects and mechanisms of three flavonoids from A. okamotoanum in the differentiated 3T3-L1 cells. The differentiated 3T3-L1 cells increased triglyceride (TG) contents, compared with non-differentiated normal group. However, treatments of three flavonoids from A. okamotoanum decreased TG contents without cytotoxicity. In addition, they showed significant down-regulation of several adipogenic transcription factors, such as γ-cytidine-cytidine-adenosine-adenosine-thymidine/enhancer binding protein -α, -β, and peroxisome proliferator-activated receptor-γ, compared with non-treated control group. Furthermore, treatment of the flavonoids inhibited expressions of lipogenesis-related proteins including fatty acid synthase, adipocyte protein 2, and glucose transporter 4. Moreover, IQ-treated group showed significant up-regulation of lipolysis-related proteins such as adipose triglyceride lipase and hormone-sensitive lipase. In addition, flavonoids significantly activated 5′-adenosine monophosphate-activated protein kinase (AMPK) compared to control group. In particular, IQ showed higher inhibition of TG accumulation by regulation of pathways related with both adipogenesis and lipolysis, than other flavonoids. The present results indicated that three flavonoids of A. okamotoanum showed anti-obesity activity by regulation of adipocyte differentiation, lipolysis, and AMPK signaling, suggesting as an anti-obesity functional agents.

Beneficial effects of carrots (Daucus carota) on adipocyte differentiation, glucose uptake, and fat accumulation

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
K Christensen ◽  
D Kotowska ◽  
L Olsen ◽  
S Bhattacharya ◽  
X Fretté ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Daucus Carota ◽  
Adipocyte Differentiation ◽  
Fat Accumulation ◽  
Beneficial Effects

New cromones from Cnidium monnieri fruits inhibit adipocyte differentiation in 3T3-L1 cells

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
SB Kim ◽  
JH Ahn ◽  
YH Jo ◽  
SH Kim ◽  
BY Hwang ◽  
...  
Keyword(s):  
Adipocyte Differentiation

The ubiquitin-like pathway neddylation controls adipocyte differentiation, obesity and metabolism

2016 ◽  
Author(s):  
Corinna Bauder ◽  
Marta Labeur ◽  
Annette Vogl ◽  
Barbara Wolfel ◽  
Wolfgang Wurst ◽  
...  
Keyword(s):  
Adipocyte Differentiation

1697-P: NFIA Differentially Regulates Adipogenesis and Myogenesis through Distinct Molecular Pathways to Ensure Adipocyte Differentiation

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 1697-P
Author(s):  
YUTA HIRAIKE ◽  
HIRONORI WAKI ◽  
KANA MIYAKE ◽  
MISATO OGUCHI ◽  
TOSHIMASA YAMAUCHI ◽  
...  
Keyword(s):  
Adipocyte Differentiation ◽  
Molecular Pathways

Inhibitory Effects of Fucoidan in 3T3-L1 Adipocyte Differentiation

2008 ◽  
Vol 11 (5) ◽  
pp. 557-562 ◽  
Author(s):  
Mi-Ja Kim ◽  
Un-Jae Chang ◽  
Jin-Sil Lee
Keyword(s):  
Adipocyte Differentiation ◽  
Inhibitory Effects

scholarly journals Genome-wide identification of cyclin-dependent kinase (CDK) genes affecting adipocyte differentiation in cattle

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Cuili Pan ◽  
Zhaoxiong Lei ◽  
Shuzhe Wang ◽  
Xingping Wang ◽  
Dawei Wei ◽  
...  
Keyword(s):  
Gene Family ◽  
Expression Analysis ◽  
Adipocyte Differentiation ◽  
Bos Taurus ◽  
Adipogenic Differentiation ◽  
Critical Role ◽  
Bos Indicus ◽  
Specific Expression ◽  
Genome Wide ◽  
A Genome

Abstract Background Cyclin-dependent kinases (CDKs) are protein kinases regulating important cellular processes such as cell cycle and transcription. Many CDK genes also play a critical role during adipogenic differentiation, but the role of CDK gene family in regulating bovine adipocyte differentiation has not been studied. Therefore, the present study aims to characterize the CDK gene family in bovine and study their expression pattern during adipocyte differentiation. Results We performed a genome-wide analysis and identified a number of CDK genes in several bovine species. The CDK genes were classified into 8 subfamilies through phylogenetic analysis. We found that 25 bovine CDK genes were distributed in 16 different chromosomes. Collinearity analysis revealed that the CDK gene family in Bos taurus is homologous with Bos indicus, Hybrid-Bos taurus, Hybrid Bos indicus, Bos grunniens and Bubalus bubalis. Several CDK genes had higher expression levels in preadipocytes than in differentiated adipocytes, as shown by RNA-seq analysis and qPCR, suggesting a role in the growth of emerging lipid droplets. Conclusion In this research, 185 CDK genes were identified and grouped into eight distinct clades in Bovidae, showing extensively homology. Global expression analysis of different bovine tissues and specific expression analysis during adipocytes differentiation revealed CDK4, CDK7, CDK8, CDK9 and CDK14 may be involved in bovine adipocyte differentiation. The results provide a basis for further study to determine the roles of CDK gene family in regulating adipocyte differentiation, which is beneficial for beef quality improvement.

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