Effect of serum from sub-healthy subjects with fatigue on the gene expression profile of skeletal muscle cells

Background: Adenosine plays the role of regulating cell differentiation, proliferation, and apoptosis in various kinds of cells through the B-cell lymphoma 2 (BCL2) pathway. Objectives: Since anti-apoptotic (BCL2) expression plays a role in controlling apoptosis in some cell lines, this study was designed to investigate whether adenosine analogue, NECA (non-selective adenosine receptors agonist), selective adenosine A2B receptor antagonist, PSB 603, and a selective adenosine A2A receptor agonist, CG21680, affect BCL2-gene expression in the skeletal muscle cells of rats. The purpose of this investigation was to test the hypothesis that CG21680 treatment would significantly intensify BCL2 gene expression in rat skeletal muscle. Methods: Flasks measuring 25 cm2 were employed in culturing the rat L6 skeletal muscle cells. After treating these differential cells, the relative mRNA expression level for the BCL2 gene, at varying conditions of treatment, was measured using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Results: From the qRT-PCR analysis results, it was concluded that BCL2 expression was markedly amplified after selective adenosine A2A receptor agonist, CGS21680 (p < 0.01) treatment. More prospective validation for the adenosine receptors’ contribution in modulating apoptosis by NECA was delivered by the outcomes from the combined pre-treatment of the cells with NECA and PSB 603. These outcomes show that when starved skeletal muscle cells are treated with a combination of NECA and 100 nM PSB 603, there was a substantial decrease in comparison to either treatment used on its own. Conclusions: This study’s results showed, for the first time, an increase in BCL2 gene expression within skeletal muscle after CGS21680 treatment. Hence, the prospective escalation in BCL2 protein expression might have a protective role to play against apoptosis and avert damage to the skeletal muscle.

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Altered ROS production, NF-κB activation and interleukin-6 gene expression induced by electrical stimulation in dystrophic mdx skeletal muscle cells

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease ◽

10.1016/j.bbadis.2015.03.012 ◽

2015 ◽

Vol 1852 (7) ◽

pp. 1410-1419 ◽

Cited By ~ 24

Author(s):

Carlos Henríquez-Olguín ◽

Francisco Altamirano ◽

Denisse Valladares ◽

José R. López ◽

Paul D. Allen ◽

...

Keyword(s):

Gene Expression ◽

Skeletal Muscle ◽

Electrical Stimulation ◽

Interleukin 6 ◽

Muscle Cells ◽

Skeletal Muscle Cells ◽

Ros Production

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Low RIP140 expression rescues insulin‐mediated FA oxidation via a rise in MCAD gene expression in skeletal muscle cells exposed to high palmitate availability

The FASEB Journal ◽

10.1096/fasebj.27.1_supplement.1202.11 ◽

2013 ◽

Vol 27 (S1) ◽

Author(s):

Silvana Constantinescu ◽

Lorraine P Turcotte

Keyword(s):

Gene Expression ◽

Skeletal Muscle ◽

Muscle Cells ◽

Skeletal Muscle Cells

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Testosterone induces up-regulation of mitochondrial gene expression in murine C2C12 skeletal muscle cells accompanied by an increase of nuclear respiratory factor-1 and its downstream effectors

Molecular and Cellular Endocrinology ◽

10.1016/j.mce.2019.110631 ◽

2020 ◽

Vol 500 ◽

pp. 110631 ◽

Cited By ~ 5

Author(s):

Lucía Pronsato ◽

Lorena Milanesi ◽

Andrea Vasconsuelo

Keyword(s):

Gene Expression ◽

Skeletal Muscle ◽

Mitochondrial Gene ◽

Muscle Cells ◽

Skeletal Muscle Cells ◽

Mitochondrial Gene Expression ◽

Nuclear Respiratory Factor ◽

Nuclear Respiratory Factor 1 ◽

Downstream Effectors ◽

C2c12 Skeletal Muscle Cells

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Neuromuscular Electrical Stimulation Induces Skeletal Muscle Fiber Remodeling and Specific Gene Expression Profile in Healthy Elderly

Frontiers in Physiology ◽

10.3389/fphys.2019.01459 ◽

2019 ◽

Vol 10 ◽

Cited By ~ 5

Author(s):

Rosa Mancinelli ◽

Luana Toniolo ◽

Ester Sara Di Filippo ◽

Christian Doria ◽

Mariangela Marrone ◽

...

Keyword(s):

Gene Expression ◽

Skeletal Muscle ◽

Electrical Stimulation ◽

Gene Expression Profile ◽

Expression Profile ◽

Muscle Fiber ◽

Neuromuscular Electrical Stimulation ◽

Skeletal Muscle Fiber ◽

Specific Gene ◽

Healthy Elderly

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Gene Expression Profiling of Leiomyoma and Myometrial Smooth Muscle Cells in Response to Transforming Growth Factor-β

Endocrinology ◽

10.1210/en.2004-1377 ◽

2005 ◽

Vol 146 (3) ◽

pp. 1097-1118 ◽

Cited By ~ 72

Author(s):

Xiaoping Luo ◽

Li Ding ◽

Jingxia Xu ◽

Nasser Chegini

Keyword(s):

Gene Expression ◽

Smooth Muscle ◽

Smooth Muscle Cells ◽

Gene Expression Profile ◽

Expression Profile ◽

Binding Protein ◽

Muscle Cells ◽

Time Dependent ◽

Early Gene ◽

A Cell

Altered expression of the TGF-β system is recognized to play a central role in various fibrotic disorders, including leiomyoma. In this study we performed microarray analysis to characterize the gene expression profile of leiomyoma and matched myometrial smooth muscle cells (LSMC and MSMC, respectively) in response to the time-dependent action of TGF-β and, after pretreatment with TGF-β type II receptor (TGF-βRII) antisense oligomer-blocking/reducing TGF-β autocrine/paracrine actions. Unsupervised and supervised assessments of the gene expression values with a false discovery rate selected at P ≤ 0.001 identified 310 genes as differentially expressed and regulated in LSMC and MSMC in a cell- and time-dependent manner by TGF-β. Pretreatment with TGF-βRII antisense resulted in changes in the expression of many of the 310 genes regulated by TGF-β, with 54 genes displaying a response to TGF-β treatment. Comparative analysis of the gene expression profile in TGF-βRII antisense- and GnRH analog-treated cells indicated that these treatments target the expression of 222 genes in a cell-specific manner. Gene ontology assigned these genes functions as cell cycle regulators, transcription factors, signal transducers, tissue turnover, and apoptosis. We validated the expression and TGF-β time-dependent regulation of IL-11, TGF-β-induced factor, TGF-β-inducible early gene response, early growth response 3, CITED2 (cAMP response element binding protein-binding protein/p300-interacting transactivator with ED-rich tail), Nur77, Runx1, Runx2, p27, p57, growth arrest-specific 1, and G protein-coupled receptor kinase 5 in LSMC and MSMC using real-time PCR. Together, the results provide the first comprehensive assessment of the LSMC and MSMC molecular environment targeted by autocrine/paracrine action of TGF-β, highlighting potential involvement of specific genes whose products may influence the outcome of leiomyoma growth and fibrotic characteristics by regulating inflammatory response, cell growth, apoptosis, and tissue remodeling.

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