scholarly journals Selection of suitable reference genes for gene expression studies in normal human ovarian tissues, borderline ovarian tumours and ovarian cancer

2016 ◽  
Vol 14 (6) ◽  
pp. 5725-5731 ◽  
Author(s):  
Olumide Ofinran ◽  
Ujjal Bose ◽  
Daniel Hay ◽  
Summi Abdul ◽  
Cristina Tufatelli ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ovarian Cancer ◽  
Reference Genes ◽  
Expression Studies ◽  
Ovarian Tumours ◽  
Normal Human ◽  
Gene Expression Studies ◽  
Suitable Reference ◽  
Selection Of

scholarly journals Author Correction: Selection of suitable reference genes for gene expression studies in myxosporean (Myxozoa, Cnidaria) parasites

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Anush Kosakyan ◽  
Gema Alama-Bermejo ◽  
Pavla Bartošová-Sojková ◽  
Ana Born-Torrijos ◽  
Radek Šíma ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reference Genes ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Suitable Reference ◽  
Selection Of

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

scholarly journals Selection of reference genes for measuring the expression of aiiO in Ochrobactrum quorumnocens A44 using RT-qPCR

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Dorota M. Krzyżanowska ◽  
Anna Supernat ◽  
Tomasz Maciąg ◽  
Marta Matuszewska ◽  
Sylwia Jafra
Keyword(s):  
Gene Expression ◽  
Quorum Sensing ◽  
Reference Genes ◽  
Significance Threshold ◽  
Expression Studies ◽  
Reverse Transcription Quantitative Pcr ◽  
Gene Expression Studies ◽  
The Stability ◽  
Selection Of

Abstract Reverse transcription quantitative PCR (RT-qPCR), a method of choice for quantification of gene expression changes, requires stably expressed reference genes for normalization of data. So far, no reference genes were established for the Alphaproteobacteria of the genus Ochrobactrum. Here, we determined reference genes for gene expression studies in O. quorumnocens A44. Strain A44 was cultured under 10 different conditions and the stability of expression of 11 candidate genes was evaluated using geNorm, NormFinder and BestKeeper. Most stably expressed genes were found to be rho, gyrB and rpoD. Our results can facilitate the choice of reference genes in the related Ochrobactrum strains. O. quorumnocens A44 is able to inactivate a broad spectrum of N-acyl homoserine lactones (AHLs) – the quorum sensing molecules of many Gram-negative bacteria. This activity is attributed to AiiO hydrolase, yet it remains unclear whether AHLs are the primary substrate of this enzyme. Using the established RT-qPCR setup, we found that the expression of the aiiO gene upon exposure to two AHLs, C6-HLS and 3OC12-HSL, does not change above the 1-fold significance threshold. The implications of this finding are discussed in the light of the role of quorum sensing-interfering enzymes in the host strains.

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