Knockdown of GluA2 induces apoptosis in non-small-cell lung cancer A549 cells through the p53 signaling pathway

Purpose:125I seeds were effective in the treatment of non-small cell lung cancer in previous research. However, the exact signaling pathway-mediated apoptosis mechanism is still unclear. The present study analyzed the effects and potential mechanisms of 125I seed on the growth and migration of A549 cells.Methods: Lung cancer A549 cells were irradiated with 125I seed for various times. MTT, invasion assay, and flow cytometry were used to detect the proliferation, invasion, and apoptosis of treated cells, respectively. A Nimblegen genome-wide expression profile chip was used to evaluate gene expression changes in 125I seed-treated A549 cells. Validation studies were performed using phosphorylated protein chip technology, Western blot, nude mouse tumor xenograft assay, and immunohistochemical experiments. All statistical analyses were performed using unpaired Student's t tests and Kruskal-Wallis test.Results: Irradiation with 125I seed inhibited A549 cell proliferation and invasion and induced apoptosis (primarily early apoptosis). Irradiation with 125I seed also caused the downregulation of p38MAPK, degradation of mouse double-minute 2 homolog (MDM2), and higher expression of p53, which eventually resulted in non-small cell lung cancer cell apoptosis.Conclusion:125I seed irradiation activated the p38MAPK/MDM2/p53 signaling pathway and promoted non-small cell lung cancer cell apoptosis. Future clinical studies targeting this signal may provide a new potential therapeutic approach for non-small cell lung cancer.

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HAX1 enhances the survival and metastasis of non‐small cell lung cancer through the AKT / mTOR and MDM2 /p53 signaling pathway

Thoracic Cancer ◽

10.1111/1759-7714.13634 ◽

2020 ◽

Vol 11 (11) ◽

pp. 3155-3167

Author(s):

Zhigang Liang ◽

Yuan Zhong ◽

Lifei Meng ◽

Yi Chen ◽

Yahui Liu ◽

...

Keyword(s):

Lung Cancer ◽

Small Cell Lung Cancer ◽

Signaling Pathway ◽

Cell Lung Cancer ◽

Small Cell ◽

Small Cell Lung ◽

P53 Signaling ◽

P53 Signaling Pathway

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Systematic Analysis Identifies NUF2 as an Immunological and Prognostic Biomarker for Non-small cell lung cancer

10.21203/rs.3.rs-789591/v1 ◽

2021 ◽

Author(s):

xia li ◽

zhongquan yi ◽

lianlian zhang ◽

jin zhou ◽

wenchun song ◽

...

Keyword(s):

Lung Cancer ◽

Small Cell Lung Cancer ◽

Signaling Pathway ◽

Cell Lung Cancer ◽

Small Cell ◽

Functional Enrichment ◽

Small Cell Lung ◽

Hub Genes ◽

P53 Signaling ◽

P53 Signaling Pathway

Abstract Background Non-small cell lung cancer (NSCLC) is a part of the most common cancers in the world. A lot of efforts have been made to clarify the etiology of non-small cell lung cancer, but the molecular mechanism of non-small cell lung cancer is still unclear. Methods In order to identify candidate genes in the occurrence and progression of non-small cell lung cancer, GSE19804 GSE118370 GSE19188 GSE27262 and GSE33532 microarray data sets were downloaded from the Gene Expression General (GEO) database. Identify differentially expressed genes (DEGS) and perform functional enrichment analysis. The protein-protein interaction network (PPI) was constructed, and the module analysis was performed using STRING and Cytoscape. A total of 562 DEGS were identified, consisting of 98 downregulated genes and 464 upregulated genes. Abundant functions and pathways of DEGS include p53 signaling pathway, Cell adhesion molecules,Leukocyte transendothelial migration,Vascular smooth muscle contraction,Complement and coagulation cascades and Axon guidance. Tumor immunity was assessed to investigate the functions of hub genes. Results Totally 562 genes were found to be dysregulated. 12 genes were considered to be the hub genes. NUF2 was considered as the potential immunotherapeutic targets with future clinical significance.12 hub genes were detected, and biological process analysis showed that these genes are mainly enriched in p53 signaling pathway,Progesterone-mediated oocyte maturation,Cell cycle, Oocyte meiosis and Cellular senescence Survival analysis shows that NUF2 may be linked to the occurrence, invasion or recurrence of non-small cell lung cancer. Conclusion NUF2 genes discovered in this study help us include the molecular mechanisms of the occurrence and progression of non-small cell lung cancer, and provide candidate targets for the diagnosis and treatment of non-small cell lung cancer.

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PTEN/pAkt/p53 signaling pathway correlates with the radioresponse of non-small cell lung cancer

International Journal of Molecular Medicine ◽

10.3892/ijmm_00000372 ◽

2010 ◽

Vol 25 (4) ◽

Cited By ~ 4

Author(s):

Kim

Keyword(s):

Lung Cancer ◽

Small Cell Lung Cancer ◽

Signaling Pathway ◽

Cell Lung Cancer ◽

Small Cell ◽

Small Cell Lung ◽

P53 Signaling ◽

P53 Signaling Pathway

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Phanginin R Induces Cytoprotective Autophagy via JNK/c-Jun Signaling Pathway in Non-Small Cell Lung Cancer A549 Cells

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520620666200414095828 ◽

2020 ◽

Vol 20 (8) ◽

pp. 982-988 ◽

Cited By ~ 1

Author(s):

Le-Le Zhang ◽

Han Bao ◽

Yu-Lian Xu ◽

Xiao-Ming Jiang ◽

Wei Li ◽

...

Keyword(s):

Lung Cancer ◽

Small Cell Lung Cancer ◽

Signaling Pathway ◽

Cell Lung Cancer ◽

Biological Activities ◽

A549 Cells ◽

Immunofluorescence Assay ◽

Small Cell ◽

Autophagic Flux ◽

Small Cell Lung

Background: Cassane-type diterpenoids are widely distributed in the medical plants of genus Caesalpinia. To date, plenty of cassane diterpenoids have been isolated from the genus Caesalpinia, and some of them were documented to exhibit multiple biological activities. However, the effects of these compounds on autophagy have never been reported. Objective: To investigate the effects and mechanisms of the cassane diterpenoids including Phanginin R (PR) on autophagy in Non-Small Cell Lung Cancer (NSCLC) A549 cells. Methods: Western blot analysis and immunofluorescence assay were performed to investigate the effects of the compounds on autophagic flux in A549 cells. The pathway inhibitor and siRNA interference were used to investigate the mechanism of PR. MTT assay was performed to detect cell viability. Results: PR treatment upregulated the expression of phosphatidylethanolamine-modified microtubule-associated protein Light-Chain 3 (LC3-II) in A549 cells. Immunofluorescence assay showed that PR treatment increased the production of red-fluorescent puncta in mRFP-GFP-LC3 plasmid-transfected cells, indicating PR promoted autophagic flux in A549 cells. PR treatment activated the c-Jun N-terminal Kinase (JNK) signaling pathway while it did not affect the classical Akt/mammalian Target of Rapamycin (mTOR) pathway. Pretreatment with the JNK inhibitor SP600125 or siRNA targeting JNK or c-Jun suppressed PR-induced autophagy. In addition, cotreatment with the autophagy inhibitor Chloroquine (CQ) or inhibition of the JNK/c-Jun signaling pathway increased PR-induced cytotoxicity. Conclusion: PR induced cytoprotective autophagy in NSCLC A549 cells via the JNK/c-Jun signaling pathway, and autophagy inhibition could further improve the anti-cancer potential of PR.

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MicroRNA‑21 promotes the proliferation, migration and invasion of non‑small cell lung cancer A549 cells by regulating autophagy activity via AMPK/ULK1 signaling pathway

Experimental and Therapeutic Medicine ◽

10.3892/etm.2018.6370 ◽

2018 ◽

Cited By ~ 2

Author(s):

Shuping Li ◽

Xiaofei Zeng ◽

Ruidong Ma ◽

Li Wang

Keyword(s):

Lung Cancer ◽

Small Cell Lung Cancer ◽

Signaling Pathway ◽

Cell Lung Cancer ◽

A549 Cells ◽

Small Cell ◽

Migration And Invasion ◽

Small Cell Lung ◽

Autophagy Activity

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WITHDRAWN: Evodiamine triggers apoptosis in human non-small cell lung cancer A549 cells via targeting MTDH-mediated signaling pathway

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2014.12.013 ◽

2014 ◽

Author(s):

Yanmei Zou ◽

Peng Zhang ◽

Feng Zhu ◽

Hua Xiong

Keyword(s):

Lung Cancer ◽

Small Cell Lung Cancer ◽

Signaling Pathway ◽

Cell Lung Cancer ◽

A549 Cells ◽

Small Cell ◽

Small Cell Lung

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The Potential Role of Cathepsin K in Non-Small Cell Lung Cancer

Molecules ◽

10.3390/molecules25184136 ◽

2020 ◽

Vol 25 (18) ◽

pp. 4136

Author(s):

Hui Yang ◽

Jasmine Heyer ◽

Hui Zhao ◽

Shengxian Liang ◽

Rui Guo ◽

...

Keyword(s):

Lung Cancer ◽

Small Cell Lung Cancer ◽

Signaling Pathway ◽

Cell Lung Cancer ◽

A549 Cells ◽

Cathepsin K ◽

Small Cell ◽

Mtor Signaling ◽

Mtor Signaling Pathway ◽

Small Cell Lung

(1) Background: Cathepsin K has been found overexpressed in several malignant tumors. However, there is little information regarding the involvement of Cathepsin K in non-small cell lung cancer (NSCLC). (2) Methods: Cathepsin K expression was tested in human NSCLC cell lines A549 and human embryo lung fibroblast MRC-5 cells using Western blot and immunofluorescence assay. Cathepsin K was transiently overexpressed or knocked down using transfection with a recombinant plasmid and siRNA, respectively, to test the effects on cell proliferation, migration, invasion, and on the mammalian target of rapamycin (mTOR) signaling pathway. (3) Results: Expression of Cathepsin K was increased significantly in A549 cells and diffused within the cytoplasm compared to the MRC-5 cells used as control. Cathepsin K overexpression promoted the proliferation, migration, and invasion of A549 cells, accompanied by mTOR activation. Cathepsin K knockdown reversed the above malignant behavior and inhibited the mTOR signaling activation, suggesting that Cathepsin K may promote the progression of NSCLC by activating the mTOR signaling pathway. (4) Conclusion: Cathepsin K may potentially represent a viable drug target for NSCLC treatment.

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