scholarly journals Comparison of different cytogenetic methods and tissue suitability for the study of chromosomes in Cimex lectularius (Heteroptera, Cimicidae)

2016 ◽  
Vol 10 (4) ◽  
pp. 731-752 ◽  
Author(s):  
David Sadílek ◽  
Robert B. Angus ◽  
František Šťáhlavský ◽  
Jitka Vilímová
Keyword(s):  
Chromosome Number ◽  
Sex Chromosomes ◽  
Cytogenetic Analysis ◽  
Cimex Lectularius ◽  
Chromosome Research ◽  
Holokinetic Chromosomes ◽  
Pros And Cons ◽  
Embryo Tissue ◽  
Mitosis And Meiosis ◽  
Cytogenetic Methods

In the article we summarize the most common recent cytogenetic methods used in analysis of karyotypes in Heteroptera. We seek to show the pros and cons of the spreading method compared with the traditional squashing method. We discuss the suitability of gonad, midgut and embryo tissue inCimexlectulariusLinnaeus, 1758 chromosome research and production of figures of whole mitosis and meiosis, using the spreading method.The hotplate spreading technique has many advantages in comparison with the squashing technique. Chromosomal slides prepared from the testes tissue gave the best results, tissues of eggs and midgut epithelium are not suitable. Metaphase II is the only division phase in which sex chromosomes can be clearly distinguished. Chromosome number determination is easy during metaphase I and metaphase II. Spreading of gonad tissue is a suitable method for the cytogenetic analysis of holokinetic chromosomes ofCimexlectularius.

New cytogenetic data on Coreoidea (Hemiptera: Heteroptera) with special reference to Coreidae

Zootaxa ◽  
2012 ◽  
Vol 3313 (1) ◽  
pp. 53 ◽  
Author(s):  
HAILIN YANG ◽  
HU LI ◽  
XUN DAI ◽  
JIAN CHANG ◽  
WANZHI CAI
Keyword(s):  
Chromosome Number ◽  
Special Reference ◽  
Sex Chromosomes ◽  
Meiotic Division ◽  
Phylogenetic Relationships ◽  
Male Meiosis ◽  
Point Of View ◽  
Diploid Chromosome Number ◽  
Holokinetic Chromosomes ◽  
The Family

Some cytogenetic aspects of six Chinese species of Coreoidea were studied. The material included five species from the familyCoreidae: Hydarella orientalis (Distant), Homoeocerus bannaensis Hsiao, Cletus graminis Hsiao & Cheng, Paradasynus lon-girostris Hsiao, Acanthocoris scaber (Linnaeus), and one species from the family Stenocephalidae: Stenocephalus femoralisReuter. All species show holokinetic chromosomes, post-reductional meiotic division of XO sex chromosomes, a pre-reduc-tional type of meiosis for autosomes and m-chromosomes, intersticial chiasmata in most autosomes, and one chiasma per biva-lent in male meiosis. In the species studied, the diploid chromosome number ranged from 13 to 21. It was 13 in S. femoralis (10+ 2m + XO), 15 in Hy. orientalis (12 + 2m + XO), 17 in Ho. bannaensis (14 + 2m + XO) and C. graminis (14 + 2m + XO), 19in P. longirostris (16 + 2m + XO), and 21 in A. scaber (18 + 2m + XO). Hy. orientalis represents the first cytogenetically stud-ied species in subfamily Hydarinae. The phylogenetic relationships among Coreoidea are briefly discussed from a cytogenetic point of view.

Genetic and cytogenetic analysis of the fruit fly Rhagoletis cerasi (Diptera: Tephritidae)

Genome ◽  
2008 ◽  
Vol 51 (7) ◽  
pp. 479-491 ◽  
Author(s):  
Ilias Kounatidis ◽  
Nikolaos Papadopoulos ◽  
Kostas Bourtzis ◽  
Penelope Mavragani-Tsipidou
Keyword(s):  
Salivary Gland ◽  
Sex Chromosomes ◽  
Cytogenetic Analysis ◽  
Polytene Chromosomes ◽  
Fruit Fly ◽  
Pest Species ◽  
Heteromorphic Sex Chromosomes ◽  
Weak Points ◽  
Heterogametic Sex ◽  
Rhagoletis Cerasi

The European cherry fruit fly, Rhagoletis cerasi , is a major agricultural pest for which biological, genetic, and cytogenetic information is limited. We report here a cytogenetic analysis of 4 natural Greek populations of R. cerasi, all of them infected with the endosymbiotic bacterium Wolbachia pipientis . The mitotic karyotype and detailed photographic maps of the salivary gland polytene chromosomes of this pest species are presented here. The mitotic metaphase complement consists of 6 pairs of chromosomes, including one pair of heteromorphic sex chromosomes, with the male being the heterogametic sex. The analysis of the salivary gland polytene complement has shown a total of 5 long chromosomes (10 polytene arms) that correspond to the 5 autosomes of the mitotic nuclei and a heterochromatic mass corresponding to the sex chromosomes. The most prominent landmarks of each polytene chromosome, the “weak points”, and the unusual asynapsis of homologous pairs of polytene chromosomes at certain regions of the polytene elements are also presented and discussed.

scholarly journals One more Microtus species with asynaptic sex chromosomes

2018 ◽  
Vol 70 (3) ◽  
pp. 443-447
Author(s):  
Jelena Blagojevic ◽  
Marija Rajicic ◽  
Vladimir Jovanovic ◽  
Tanja Adnadjevic ◽  
Ivana Budinski ◽  
...  
Keyword(s):  
Chromosome Number ◽  
Sex Chromosomes ◽  
Diploid Chromosome Number ◽  
First Finding ◽  
Chromosomal Polymorphisms ◽  
Southeastern Serbia

Arvicoline voles are known as a karyotypically extremely polymorphic group in which the genus Microtus leads with the highest rate of karyotype change. A member of this genus, the European pine vole Microtus subterraneus (de Selys-Longchamps, 1836), is widely distributed in Europe and parts of Asia. There are two cytotypes differing in diploid chromosome number, 2n=54 and 52, each showing different chromosomal polymorphisms. At two localities in southeastern Serbia, Mt. Jastrebac and Vlasina, we found the 2n=52 cytotype. Meiotic preparations from males revealed the presence of asynaptic sex chromosomes. Although asynaptic sex chromosomes are frequent in Microtus, this is the first finding for M. subterraneus. From summarized data it appears that two-thirds of the studied species, mainly from Microtus and Terricola subgenera, possess asynaptic sex chromosomes.

scholarly journals High Chromosome Number and Giant Postreductional Sex Chromosomes in the Beetle Walterianella venusta Schaufuss (Chrysomelidae, Alticinae)

1969 ◽  
Vol 47 (3) ◽  
pp. 154-163
Author(s):  
Niilo Virkki
Keyword(s):  
Chromosome Number ◽  
Sex Chromosomes ◽  
High Chromosome Number

A high chromosome number for a coleopteran, 22-J-X+y, was encountered in an alticine, Walterianella venusta Schaufuss. The autosomes are very short, metacentric; the sex chromosomes very long and also metacentric. The sex chromosomes are desynaptic and postreductional in spermatogenesis. It is assumed that two trends operate in the evolution of the alticine chromosomes, first, the autosomal chromatin being incorporated repeatedly in the sex chromosomes, and second, the occurrence of the multiplication of the short autosomes.

KARYOLOGICAL RELATIONSHIPS IN TURTLES (REPTILIA: CHELONIA)

1972 ◽  
Vol 14 (4) ◽  
pp. 859-868 ◽  
Author(s):  
A. Dean Stock
Keyword(s):  
Chromosome Number ◽  
Sex Chromosomes ◽  
Mitotic Chromosomes ◽  
Meiotic Chromosomes ◽  
Telocentric Chromosomes ◽  
Chromosome Karyotype

The mitotic chromosomes of 33 species of chelonians representing 22 genera and six families were investigated. Chromosome number and morphology are the same for most members of a given family and range from 66 in Trionyx to 34 in Pelomedusa. Most emydid genera have 50 chromosomes. The karyotype of Chelydra (2n = 52) is similar to those of some testudinid and emydid genera and is unlike the 56 chromosome karyotype of kinosternid turtles. The three genera of tortoises examined, Gopherus, Testudo, and Geochelone, have 52 chromosomes, but Gopherus differs in karyotypic details. The karyotype of Geochelone is like that of Chelydra and the 52 chromosome genera of emydid turtles. The African pleurodiran Pelomedusa has three additional pairs of small acrocentric or telocentric chromosomes not present in the earlier described karyotype of Podocnemis. Examination of meiotic chromosomes revealed frequencies of chiasmata formation similar to those reported earlier. Sex chromosomes were not distinguishable.

First Record on Sex Chromosomes in a Species of the Family Cynodontidae: Cynodon gibbus (Agassiz, 1829)

2020 ◽  
Vol 160 (1) ◽  
pp. 29-37 ◽  
Author(s):  
Vanessa S. Pinheiro Figliuolo ◽  
Leonardo Goll ◽  
Patrik Ferreira Viana ◽  
Eliana Feldberg ◽  
Maria C. Gross
Keyword(s):  
Chromosome Number ◽  
Repetitive Dna ◽  
Sex Chromosomes ◽  
First Record ◽  
Diploid Chromosome Number ◽  
Fish Family ◽  
Female Heterogamety ◽  
The Family

The fish family Cynodontidae belongs to the superfamily Curimatoidea, together with the Hemiodontidae, Serrasalmidae, Parodontidae, Prochilodontidae, Chilodontidae, Curimatidae, and Anostomidae. The majority of the species of this superfamily that have been analyzed to date have a diploid chromosome number of 2n = 54. Differentiated sex chromosomes (with female heterogamety) have been observed only in the Prochilodontidae, Parodontidae, and Anostomidae. The present study provides the first description of differentiated sex chromosomes in the cynodontid species Cynodon gibbus, which has a ZZ/ZW system, and shows that repetitive DNA has played a fundamental role in the differentiation of these sex chromosomes.

scholarly journals The Role of Cytogenetic Methods in the Diagnosis of Haematological Diseases

2021 ◽  
Author(s):  
Amina Aščerić ◽  
Mirela Mačkić Durović ◽  
Hilada Nefić
Keyword(s):  
Chromosomal Aberrations ◽  
Cytogenetic Analysis ◽  
Chromosomal Abnormalities ◽  
Haematological Malignancies ◽  
Diagnosis And Prognosis ◽  
Conventional Cytogenetic Analysis ◽  
Frequent Use ◽  
Conventional Cytogenetics ◽  
Cytogenetic Methods

Introduction: Conventional cytogenetics by the use of standard karyotyping allows the study of numerical and structural chromosomal aberrations. Haematological malignancies include a number of cancer types that originate in the blood cells of the bone marrow or of the lymphatic system. Cytogenetic methods are traditionally used for the sake of diagnosis and prognosis of these diseases. However, with the ever more frequent use of molecular methods in the diagnostic laboratories, the importance of the conventional cytogenetic analysis in the diagnosis of haematological diseases needs to be reassessed. Aim: To evaluate the role of cytogenetic methods in the diagnosis of haematological malignancies. Materials and Methods: A retrospective analysis of cytogenetic findings of 146 patients with various haematological malignancies was performed. All of the findings were made over a period of three years at the Centre for Genetics at the Medical Faculty of the University of Sarajevo in Bosnia and Herzegovina. Microsoft Excel 2019 was used for the analysis and presentation of data in the form of tables and graphs. Results: The results of the present study showed that the use of conventional cytogenetic analysis is a good diagnostic method for 50.68% (74) of patients in whom chromosomal aberrations were detected. Conclusion: Cytogenetics remains the most comprehensive method for assessing chromosomal abnormalities due to its ability to detect clinically relevant aneuploidies and additional cytogenetic abnormalities that cannot be detected by locus-specific assays.

scholarly journals Determination of somatic and sex chromosomes of three Egyptian birds species using cytogenetic analysis

Genetika ◽  
2017 ◽  
Vol 49 (1) ◽  
pp. 285-295 ◽  
Author(s):  
A.H. Abu-Almaaty
Keyword(s):  
Sex Chromosomes ◽  
Chromosome Numbers ◽  
Related Species ◽  
Cytogenetic Analysis ◽  
Morphometric Measurement ◽  
Evolutionary Patterns ◽  
Taxonomic Relationships ◽  
Corvus Ruficollis

Study of karyotypes has been revealing important information on the taxonomic relationships and evolutionary patterns in various groups of birds. Karyotypes analysis and morphometric measurement of the chromosomes of three birds species of Geopelia cuneata (Columbiformes), Oriolus oriolus and Corvus ruficollis (Passeriformes) and their karyological have been studied. The diploid chromosome numbers of three species were, 2n=72, 2n=80 and 2n =80 respectively. The karyotypes of three species were different. The somatic and sex chromosomes and karyotypes of three species discussed and compared with other related species in the present study.

scholarly journals Karyotype and genome size determination of Jarilla chocola, an additional sister clade of Carica papaya

Plant Omics ◽  
2021 ◽  
pp. 50-56
Author(s):  
Dessireé Patricia Zerpa-Catanho ◽  
Tahira Jatt ◽  
Ray Ming
Keyword(s):  
Chromosome Number ◽  
Genome Size ◽  
Sex Chromosomes ◽  
Dna Content ◽  
Carica Papaya ◽  
Haploid Genome ◽  
Diploid Genome ◽  
Significant Difference ◽  
Heteromorphic Sex Chromosomes

Jarilla chocola is an herbaceous plant species that belongs to the Jarilla genus and the Caricaceae family. No information on chromosome number or genome size has been reported for J. chocola that confirms the occurrence of dysploidy events and explore the existence of heteromorphic sex chromosomes. Therefore, the total number of chromosomes of this species was determined by karyotyping and counting the number of chromosomes observed, and the genome size of female and male plants was estimated separately by flow cytometry. Results showed that J. chocola has eight pairs of chromosomes (2n = 2x = 16), and its chromosomes are classified as metacentric for five pairs, submetacentric for two pairs and telocentric for one pair. The nuclear DNA content (1C-value) in picograms and diploid genome size was estimated separately from female and male plants using two species as the standards, Phaseolus vulgaris (1C = 0.60 pg) and Carica papaya (1C = 0.325 pg), to look for the possible existence of heteromorphic sex chromosomes. C. papaya proved to be a better standard for the determination of J. chocola DNA content and diploid genome size. No significant difference on the DNA content was observed between female (1C = 1.02 ± 0.003 pg) and male (1C = 1.02 ± 0.008 pg) plants. The estimated genome size of J. chocola per haploid genome in base pairs was calculated from the obtained C-values. Results showed an estimated genome size per haploid genome of 1018.44 ± 3.07 Mb and 1022.08 ± 7.76 Mb for female and male plants, respectively. Due to the observed chromosome number and genome size, only the occurrence of one of two previously reported dysploidy events in Jarilla could be confirmed for J. chocola and no evidence of heteromorphic sex chromosomes was found. These results provide fundamental information of the J. chocola genome and will expedite investigation of sex chromosomes and genome evolution in this species, the Jarilla genus and the Caricaceae family

Sign in / Sign up

Export Citation Format

Share Document