scholarly journals Lake Poso's shrimp fauna revisited: the description of five new species of the genus Caridina (Crustacea, Decapoda, Atyidae) more than doubles the number of endemic lacustrine species

ZooKeys ◽  
2021 ◽  
Vol 1009 ◽  
pp. 81-122
Author(s):  
Werner Klotz ◽  
Thomas von Rintelen ◽  
Daisy Wowor ◽  
Chris Lukhaup ◽  
Kristina von Rintelen
Keyword(s):  
New Species ◽  
Incomplete Lineage Sorting ◽  
Taxonomic Revision ◽  
Morphological Characters ◽  
Integrative Approach ◽  
Species Flock ◽  
Lineage Sorting ◽  
Introgressive Hybridisation ◽  
First Case ◽  
Eleven Species

Lake Poso, an ancient lake system on the Indonesian island Sulawesi, harbours an endemic species flock of six, four lacustrine and two riverine species of the freshwater shrimp genus Caridina. In this study, five new lacustrine species are described, bringing the total to eleven species altogether. The number of lacustrine species is more than doubled to nine species compared to the last taxonomic revision in 2009. One of them, Caridina mayamareenae Klotz, Wowor & von Rintelen, sp. nov., even represents the first case of an atyid shrimp associated with freshwater snails which is morphologically adapted to living in shells. An integrative approach was used by providing a combination of morphological, ecological, and molecular data. Based on standard morphological characters, distribution, substrate preferences, and colouration of living specimens in the field, five distinct undescribed species could be distinguished. To support our species-hypothesis based on the mitochondrial genes 16S and COI, a molecular phylogeny was used for all eleven species from Lake Poso. All species form a well-supported monophyletic group, but only four morphospecies consistently correspond to mtDNA clades – a possible reason could be introgressive hybridisation, incomplete lineage sorting, or not yet fixed species boundaries. These results are discussed further in the context of adaptive radiation, which turned out to be more diverse than previously described. Finally, yet importantly, subjecting all new species to similar threats and to the same IUCN category and criterion than the previously described species from the lake is recommended.

scholarly journals Tardigrades of Kristianstads Vattenrike Biosphere Reserve with description of four new species from Sweden

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Edoardo Massa ◽  
Roberto Guidetti ◽  
Michele Cesari ◽  
Lorena Rebecchi ◽  
K. Ingemar Jönsson
Keyword(s):  
New Species ◽  
Laser Scanning ◽  
New Records ◽  
Biosphere Reserve ◽  
Morphological Characters ◽  
Sem Analysis ◽  
Morphological Data ◽  
Integrative Approach ◽  
Confocal Laser ◽  
Biological Value

AbstractKristianstads Vattenrike Biosphere Reserve [KVBR] is a UNESCO designated area of Sweden possessing high biological value. Although several studies on tardigrades inhabiting Sweden have been performed, the KVBR area has been neglected. The current study investigates the tardigrade fauna of five areas of the biosphere reserve and includes 34 samples of different substrates analysed quantitatively and qualitatively. In total, 33 species of tardigrades were found in the samples, including 22 new records for the Skåne region, 15 new records for Sweden, and four species new to science. Mesobiotus emiliae sp. nov., Xerobiotus gretae sp. nov., Itaquascon magnussoni sp. nov., and Thulinius gustavi sp. nov. were described with an integrative approach (when possible) using morphological characters (light, electron scanning, and confocal laser scanning microscopies) and molecular markers (ITS2, 18S, 28S, cox1). A new protocol to increase morphological data was developed recovering mounted specimens within old slides for SEM analysis. Emended diagnoses for the genus Itaquascon and the transfer of Platicrista itaquasconoide to the genus Meplitumen are proposed. This study enriches the knowledge of the tardigrade biodiversity both within the KVBR and in Sweden and contributes to the rapidly increasing number of tardigrade species reported worldwide. The 33 species identified in the KVBR area represents 28% of all water bear species found in Sweden so far. The restricted study areas and limited number of samples collected suggests that the KVBR is very rich of tardigrades.

Taxonomic revision of species of Haematoloechus Looss, 1899 (Digenea: Plagiorchioidea), with molecular phylogenetic analysis and the description of three new species from Mexico

Zootaxa ◽  
2018 ◽  
Vol 4526 (3) ◽  
pp. 251 ◽  
Author(s):  
VIRGINIA LEÓN-RÈGAGNON ◽  
JANET TOPAN
Keyword(s):  
New Species ◽  
Taxonomic Revision ◽  
Morphological Characters ◽  
Nomen Nudum ◽  
Phylogenetic Hypothesis ◽  
Molecular Phylogenetic ◽  
Phylogenetic Hypotheses ◽  
Three New Species ◽  
Host Parasite ◽  
Species Inquirendae

Lung flukes of the genus Haematoloechus Looss, 1899 are common parasites of anurans worldwide, but the taxonomy of the group has been confusing. In this taxonomic revision, 89 species of Haematoloechus (= Pneumonoeces Looss, 1902, Ostioloides Odening, 1960, Ostiolum Pratt, 1903, Skrjabinoeces Sudarikov, 1950, Neohaematoloechus Odening, 1960, Metahaematoloechus Yamaguti, 1971) are listed. Of these, 70 are considered valid, three are species inquirendae (H. legrandi Mañé–Garzón & Gil, 1959, H. latoricensis Kozák, 1968 & H. vitelloconfluentum (Rai, 1962) Saeed, Al–Barwari & Al-Harmni, 2007), one is a nomen nudum H. sudarikovi Belouss, 1962, 14 are junior synonyms and one belongs to Ostioloides. This publication also describes three new species, H. occidentalis n. sp., H. veracruzanus n. sp. and H. mexicanus n. sp., parasitizing species of Rana Linnaeus in Mexico and redescribes Haematoloechus caballeroi (Skrjabin & Antipin, 1962) Yamaguti, 1971. The phylogenetic hypotheses based on sequences of mitochondrial and ribosomal DNA of Haematoloechus spp. show that genera proposed on the basis of morphological characters are not supported. The host records for species of Haematoloechus, together with the phylogenetic hypothesis of the genus, suggest that this host-parasite association predates the ranid diversification in the Cretaceous. 

scholarly journals A taxonomic revision of the family Harrimaniidae (Hemichordata: Enteropneusta) with descriptions of seven species from the Eastern Pacific

Zootaxa ◽  
2010 ◽  
Vol 2408 (1) ◽  
pp. 1 ◽  
Author(s):  
C. DELAND ◽  
C. B. CAMERON ◽  
K. P. RAO ◽  
W. E. RITTER ◽  
T. H. BULLOCK
Keyword(s):  
New Species ◽  
Taxonomic Revision ◽  
Morphological Characters ◽  
Eastern Pacific ◽  
Nomen Nudum ◽  
Phylogenetic Hypothesis ◽  
Number Of Species ◽  
Dichotomous Key ◽  
The Family ◽  
The Status

The family Harrimaniidae (Hemichordata: Enteropneusta) is revised on the basis of morphological characters. The number of harrimaniid genera is increased to nine by the addition of Horstia n. gen., Mesoglossus n. gen., Ritteria n. gen. and Saxipendium, a genus previously assigned to the monospecific family Saxipendiidae. The number of species is increased to 34, resulting from the description of five new species from the eastern Pacific — Horstia kincaidi, Mesoglossus intermedius, M. macginitiei, Protoglossus mackiei and Ritteria ambigua. A description is supplied for a sixth harrimaniid species, Stereobalanus willeyi Ritter & Davis, 1904, which previously had the status of a nomen nudum. Four harrimaniids previously assigned to the genus Saccoglossus are transfered to the genus Mesoglossus — M. bournei, M. caraibicus, M. gurneyi and M. pygmaeus, while Saccoglossus borealis is reassigned to the genus Harrimania. Notes on habitat and zoogeography are included for the seven foregoing species and a table of diagnostic characters for existing and new species and a dichotomous key to the enteropneust families and harrimaniid genera are provided. Finally, a phylogenetic hypothesis concerning the Harrimaniidae is postulated, with discussion on the evolution of the group.

A new riparian Mantidactylus (Brygoomantis) frog from the Tsaratanana and Manongarivo Massifs in northern Madagascar

Zootaxa ◽  
2018 ◽  
Vol 4486 (4) ◽  
pp. 575 ◽  
Author(s):  
MIGUEL VENCES ◽  
ANDREA HILDENBRAND ◽  
KATHARINA M. WARMUTH ◽  
FRANCO ANDREONE ◽  
FRANK GLAW
Keyword(s):  
New Species ◽  
Nuclear Dna ◽  
Taxonomic Revision ◽  
Morphological Characters ◽  
Molecular Data ◽  
Test Case ◽  
Nominal Species ◽  
Type Specimens ◽  
The Status ◽  
Morphological Differences

The subgenus Brygoomantis in the Madagascar-endemic genus Mantidactylus contains 12 nominal species but is in urgent need of taxonomic revision as many additional, genetically divergent but undescribed candidate species have been identified. We here take a first step towards a better resolution of this group by describing a new species, Mantidactylus schulzi sp. nov., occurring at the Tsaratanana and Manongarivo Massifs, differentiated in genetic, bioacoustic and sometimes morphological characters from its closest relatives. We show that upon detailed study, most species in Brygoomantis can be delimited by concordant differentiation of mitochondrial and nuclear DNA, and by bioacoustic and morphological differences. We flag this group of morphologically similar frogs as a test case where molecular data on historical type specimens by ancient DNA methods might be needed to reach a satisfying clarification of taxonomy and nomenclature. However, the status of the new species M. schulzi is not in doubt as it is morphologically distinct from most historical type specimens, and microendemic to a region in northern Madagascar from where no earlier names exist. 

scholarly journals Three new species of Leptanilloides Mann from Andean Ecuador (Formicidae: Leptanilloidinae)

Zootaxa ◽  
2006 ◽  
Vol 1201 (1) ◽  
pp. 47 ◽  
Author(s):  
DAVID A. DONOSO ◽  
JUAN M. VIEIRA ◽  
ALEXANDER L. WILD
Keyword(s):  
New Species ◽  
Phenotypic Variation ◽  
Morphological Characters ◽  
Generic Diagnosis ◽  
Army Ant ◽  
First Case ◽  
Three New Species ◽  
Worker Caste

We report three new species of the ant genus Leptanilloides from Andean Ecuador. Leptanilloides nomada sp. n. and L. caracola sp. n. are described from the worker caste, the latter from a unique specimen; L. nubecula sp. n. is described from workers, males, and two gyne subcastes previously unknown for the entire subfamily. Phenotypic variation in morphological characters in the new species L. nomada and L. nubecula is more plastic than suggested by the traditional generic diagnosis. These records represent the first case of three Leptanilloides species occurring in sympatry. We discuss aspects of the army ant syndrome and its relevance to the genus Leptanilloides.Tres nuevas especies del género de hormigas Leptanilloides son descritas a partir de especimenes encontrados en Los Andes ecuatorianos. Leptanilloides nomada sp. n. y L. caracola sp. n. son descritas en base a obreras, ésta última es descrita a partir de un sólo espécimen. L. nubecula sp. n. es descrita en base a obreras, machos y dos subcastas de ginas previamente desconocidas para toda la subfamilia. La variación fenotípica encontrada en algunos caracteres morfológicos de las nuevas especies L. nomada y L. nubecula es más plástica de lo que sugiere la diagnosis genérica tradicional. Estos registros constituyen el primer ejemplo de tres especies de Leptanilloides viviendo en simpatría. Discutimos aspectos del síndrome de las hormigas legionarias y su aplicación al género Leptanilloides.

Taxonomic revision of the “mexicanus” group of the genus Vaejovis C. L. Koch, 1836 (Scorpiones: Vaejovidae)

Zootaxa ◽  
2019 ◽  
Vol 4596 (1) ◽  
pp. 1 ◽  
Author(s):  
CONTRERAS-FÉLIX GERARDO A. ◽  
FRANCKE B. OSCAR F.
Keyword(s):  
New Species ◽  
Natural History ◽  
Taxonomic Revision ◽  
Morphological Characters ◽  
The Other ◽  
Species Groups ◽  
Males And Females ◽  
First Time

Within the scorpion genus Vaejovis C.L. Koch, the “mexicanus” group is composed of species distributed in the mountains of México. This group presents taxonomic problems, because its characterization and the species included in the group have varied through the years. In the present work, we redefine this group based on several morphological characters, and we differentiate it from the other two species groups within the genus: “vorhiesi” and “nit dulus+nigrescens”. Additionally, five new species are described: Vaejovis ceboruco sp. nov., Vaejovis nanchititla sp. nov., Vaejovis santibagnezi sp. nov., Vaejovis talpa sp. nov. and Vaejovis tapalpa sp. nov; the males of three species are described for the first time (V. dugesi, V. nigrofemoratus and V. tesselatus); and the updated diagnosis for all species is included. Keys for the identification of males and females of the 30 species included in this group are given. Lastly, notes on the natural history and distribution of some species are provided, with maps of known distribution for all the species.

A revision of the genus Bubastes Laporte & Gory, 1836 (Coleoptera: Buprestidae)

Zootaxa ◽  
2020 ◽  
Vol 4832 (1) ◽  
pp. 1-75
Author(s):  
SVATOPLUK BÍLÝ ◽  
MARK HANLON
Keyword(s):  
New Species ◽  
Western Australia ◽  
New South ◽  
New South Wales ◽  
South Australia ◽  
Taxonomic Revision ◽  
Morphological Characters ◽  
Northern Territory ◽  
New Synonyms ◽  
South Wales

Taxonomic revision of the genus Bubastes Laporte & Gory, 1836. Thirteen new species are described: Bubastes barkeri sp. nov. (New South Wales, Queensland, Victoria), B. deserta sp. nov. (South Australia), B. dichroa sp. nov. (Western Australia), B. flavocaerulea sp. nov. (New South Wales, Queensland), B. hasenpuschi sp. nov. (Queensland), B. iridiventris sp. nov. (Western Australia), B. iris sp. nov. (Western Australia), B. macmillani sp. nov. (Western Australia), B. magnifica sp. nov. (Queensland, New South Wales), B. michaelpowelli sp. nov. (Western Australia), B. pilbarensis sp. nov. (Western Australia), B. remota sp. nov. (Northern Territory) and B. viridiaurea sp. nov. (Western Australia). The following seventeen new synonyms are proposed: Bubastes thomsoni Obenberger, 1928, syn. nov. = B. australasiae Obenberger, 1922, B. olivina Obenberger, 1920, syn. nov. = Neraldus bostrychoides Théry 1910, B. boisduvali Obenberger, 1941, syn. nov. = B. erbeni Obenberger, 1941, B. borealis Obenberger, 1941, syn. nov. = B. globicollis Thomson, 1879, B. laticollis Blackburn, 1888, syn. nov. = B. globicollis Thomson, 1879, B. simillima Obenberger, 1922, syn. nov. = B. globicollis Thomson, 1879, B. obscura Obenberger, 1922, syn. nov. = B. inconsistans Thomson, 1879, B. septentrionalis Obenberger, 1941, syn. nov. = B. inconsistans Thomson, 1879, B. viridicupraea Obenberger, 1922, syn. nov. = B. inconsistans Thomson, 1879, B. blackburni Obenberger, 1941, syn. nov. = B. kirbyi Obenberger, 1928, B. chapmani Obenberger, 1941, syn. nov. = B. kirbyi Obenberger, 1928, B. aenea Obenberger, 1922, syn. nov. = B. niveiventris Obenberger, 1922, B. saundersi Obenberger, 1928, syn. nov. = B. odewahni Obenberger, 1928, B. occidentalis Blackburn, 1891, syn. nov. = B. sphaenoida Laporte & Gory, 1836, B. persplendens Obenberger, 1920, syn. nov. = B. sphaenoida Laporte & Gory, 1836, B. splendens Blackburn, 1891, syn. nov. = B. sphaenoida Laporte & Gory, 1836 and B. strandi Obenberger, 1920, syn. nov. = B. suturalis Carter, 1915. Neotype is designated and redescribed for Bubastes cylindrica W. J. Macleay, 1888 and lectotypes are designated for Bubastes thomsoni Obenberger, 1928 and B. leai Carter, 1924. Morphological characters of the genus are presented and all species are illustrated (incl. historical types) and a key is provided for all species of the genus. 

Integrative approach reveals underestimated Peyssonneliales diversity in Brazil: registering the first occurrence of Ramicrusta and Incendia, with the description of three new species

Phytotaxa ◽  
2020 ◽  
Vol 439 (1) ◽  
pp. 39-55
Author(s):  
EDILENE MARIA DOS SANTOS PESTANA ◽  
GOIA DE MATTOS LYRA ◽  
GABRIEL DO NASCIMENTO SANTOS ◽  
CIBELE CONCEIÇÃO DOS SANTOS ◽  
VALÉRIA CASSANO ◽  
...  
Keyword(s):  
New Species ◽  
Original Description ◽  
Morphological Characters ◽  
Type Locality ◽  
Integrative Approach ◽  
Brazilian Coast ◽  
Monophyletic Clade ◽  
Sister Relationship ◽  
First Occurrence ◽  
Three New Species

Peyssonneliaceae represents a monophyletic clade of red encrusting algae classified Peyssonneliales. Molecular analyses have been important for revealing unknown diversity in this family, which includes small, cryptic organisms, frequently distinguished solely by discrete anatomical characters. Brazil however, remains virtually unknown. To assess this diversity, phylogenetic and barcoding analyses using rbcL, COI-5P and SSU data, as well as morpho-anatomical analyses were performed. Here we report the first occurrence of two genera on the Brazilian coast: Ramicrusta and Incendia, and describe three new species: Ramicrusta fujiiana sp. nov., Ramicrusta paradoxa sp. nov. and Incendia yoneshigueana sp. nov. Ramicrusta was resolved as a monophyletic clade in the three reconstructed phylogenies, and had congruent topologies. In the rbcL phylogeny, Ramicrusta fujiiana formed a well-supported clade sister to a well-supported clade including R. aranea (Vanuatu, type locality) and R. textilis (Jamaica, type locality). Morphological characters of R. paradoxa are not in agreement with the original description of the genus. Therefore, we present an updated and broader concept of Ramicrusta. Incendia was resolved as a monophyletic clade with full support for rbcL. Incendia yoneshigueana is in a sister relationship with I. glabra (Vanuatu, type locality). This study extends the distributions of Ramicrusta and Incendia, proposes three new species, and confirms the effectiveness of rbcL, COI-5P and SSU in inferring phylogenetic relationships and delimiting species in the Peyssonneliales. The integrative approach established clear taxonomic concepts for the studied genera and species, and identified useful characters for genera delimitation in Peyssonneliaceae.

Corrigendum to: Systematics and distribution of world hyptiogastrine wasps (Hymenoptera : Gasteruptiidae)

2002 ◽  
Vol 16 (6) ◽  
pp. 957 ◽  
Author(s):  
J. T. Jennings ◽  
A. D. Austin
Keyword(s):  
New Species ◽  
New Zealand ◽  
South America ◽  
New Caledonia ◽  
Cladistic Analysis ◽  
Taxonomic Revision ◽  
Morphological Characters ◽  
The Family ◽  
New Hebrides ◽  
New Britain

This study examines the phylogeny, taxonomy, distribution and biology of the gasteruptiid subfamily Hyptiogastrinae and, at the same time, presents an overview of the family. Following a cladistic analysis of 35 discrete morphological characters, two monophyletic genera are recognised, Hyptiogaster Kieffer and Pseudofoenus s. l. Kieffer. As a consequence, the genera Aulacofoenus Kieffer, Crassifoenus Crosskey, and Eufoenus Szépligeti are synonymised with Pseudofoenus. A total of 88 species are recognised for the subfamily, 10 species of Hyptiogaster, which are restricted to mainland Australia, and 78 species of Pseudofoenus, 40 of which are described as new. Pseudofoenus has a restricted Gondwanan distribution and is found in Australia including Tasmania (65 spp.), New Guinea and New Britain (5 spp.), the south-west Pacific (New Caledonia, New Hebrides and Fiji – 2 spp.), New Zealand (4 spp.) and South America (2 spp.). No new species have been recorded from either New Zealand or South America. For Pseudofoenus, information on the distribution of each species, their biology (if known) and an identification key are presented.Following a taxonomic revision, the following new species are described: P. baileyi, sp. nov., P. baitetaensis, sp. nov., P. beverlyae, sp. nov., P. caperatus, sp. nov., P. cardaleae, sp. nov., P. carrabinensis, sp. nov., P. claireae, sp. nov., P. collessi, sp. nov., P. coorowensis, sp. nov., P. crosskeyi, sp. nov., P. douglasorum, sp. nov., P. eliseae, sp. nov., P. ericae, sp. nov., P. eustonensis, sp. nov., P. feckneri, sp. nov., P. gressitti, sp. nov., P. gullanae, sp. nov., P. hackeri, sp. nov., P. imbricatus, sp. nov., P. iqbali, sp. nov., P. kadowi, sp. nov., P. karimuiensis, sp. nov., P. kelleri, sp. nov., P. leinsterensis, sp. nov., P. macdonaldi, sp. nov., P. malkini, sp. nov., P. marshalli, sp. nov., P. masneri, sp. nov., P. mitchellae, sp. nov., P. morganensis, sp. nov., P. nalbarraensis, sp. nov., P. pumilis, sp. nov., P. schmidti, sp. nov., P. stevensi, sp. nov., P. tasmaniensis, sp. nov., P. taylori, sp. nov., P. umboiensis, sp. nov., P. walkeri, sp. nov. and P. zborowskii, sp. nov. The synonymy of Aulacofoenus, Crassifoenus and Eufoenus with Pseudofoenus result in the following new combinations: from Aulacofoenus: P. bungeyi (Jennings & Austin), comb. nov., P. deletangi (Schletterer), comb. nov., P. fallax (Schletterer), comb. nov., P. fletcheri (Jennings & Austin), comb. nov., P. goonooensis (Jennings & Austin), comb. nov., P. infumatus (Schletterer), comb. nov., P. kurmondi (Jennings & Austin), comb. nov., P. loxleyi (Jennings & Austin), comb. nov., P. marionae (Jennings & Austin), comb. nov., P. perenjorii (Jennings & Austin), comb. nov., P. swani (Jennings & Austin), comb. nov., P. thoracicus (Guérin Menéville), comb. nov., P. whiani (Jennings & Austin), comb. nov. and P. wubinensis (Jennings & Austin), comb. nov.; from Crassifoenus: P. houstoni (Jennings & Austin), comb. nov., P. grossitarsis (Kieffer), comb. nov and P. macronyx (Schletterer), comb. nov.; and from Eufoenus: P. antennalis (Schletterer), comb. nov., P. australis (Westwood), comb. nov., P. crassitarsis (Kieffer), comb. nov., P. darwini (Westwood), comb. nov., P. extraneus (Turner), comb. nov., P. ferrugineus (Crosskey), comb. nov., P. floricolus (Turner), comb. nov., P. inaequalis (Turner), comb. nov., P. melanopleurus (Crosskey), comb. nov., P. minimus (Turner), comb. nov., P. nitidiusculus (Turner), comb. nov., P. patellatus (Westwood), comb. nov., P. pilosus (Kieffer), comb. nov., P. reticulatus (Crosskey), comb. nov., P. rieki (Crosskey), comb. nov., P. ritae (Cheesman), comb. nov. and P. spinitarsis (Westwood), comb. nov. Pseudofoenus microcephalus (Crosskey), comb. nov. is transferred from Hyptiogaster and Eufoenus flavinervis (Kieffer) remains incertae sedis.

scholarly journals Phylogeny and Species Delimitation of Chinese Medicago (Leguminosae) and Its Relatives Based on Molecular and Morphological Evidence

2021 ◽  
Vol 11 ◽  
Author(s):  
Jinyuan Chen ◽  
Guili Wu ◽  
Nawal Shrestha ◽  
Shuang Wu ◽  
Wei Guo ◽  
...  
Keyword(s):  
Phylogenetic Relationship ◽  
Species Delimitation ◽  
Large Scale ◽  
Incomplete Lineage Sorting ◽  
Phylogenetic Analyses ◽  
Population Based ◽  
Integrative Approach ◽  
Morphological Evidence ◽  
Sampling Errors ◽  
Lineage Sorting

Medicago and its relatives, Trigonella and Melilotus comprise the most important forage resources globally. The alfalfa selected from the wild relatives has been cultivated worldwide as the forage queen. In the Flora of China, 15 Medicago, eight Trigonella, and four Melilotus species are recorded, of which six Medicago and two Trigonella species are introduced. Although several studies have been conducted to investigate the phylogenetic relationship within the three genera, many Chinese naturally distributed or endemic species are not included in those studies. Therefore, the taxonomic identity and phylogenetic relationship of these species remains unclear. In this study, we collected samples representing 18 out of 19 Chinese naturally distributed species of these three genera and three introduced Medicago species, and applied an integrative approach by combining evidences from population-based morphological clusters and molecular data to investigate species boundaries. A total of 186 individuals selected from 156 populations and 454 individuals from 124 populations were collected for genetic and morphological analyses, respectively. We sequenced three commonly used DNA barcodes (trnH-psbA, trnK-matK, and ITS) and one nuclear marker (GA3ox1) for phylogenetic analyses. We found that 16 out of 21 species could be well delimited based on phylogenetic analyses and morphological clusters. Two Trigonella species may be merged as one species or treated as two subspecies, and Medicago falcata should be treated as a subspecies of the M. sativa complex. We further found that major incongruences between the chloroplast and nuclear trees mainly occurred among the deep diverging lineages, which may be resulted from hybridization, incomplete lineage sorting and/or sampling errors. Further studies involving a finer sampling of species associated with large scale genomic data should be employed to better understand the species delimitation of these three genera.

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