scholarly journals Two new species of the Balkan genus Paladilhiopsis Pavlović, 1913 (Caenogastropoda, Moitessieriidae)

ZooKeys ◽  
2021 ◽  
Vol 1046 ◽  
pp. 157-176
Author(s):  
Sebastian Hofman ◽  
Jozef Grego ◽  
Aleksandra Rysiewska ◽  
Artur Osikowski ◽  
Andrzej Falniowski
Keyword(s):  
Balkan Peninsula ◽  
Reproductive Organs ◽  
Phylogeny Reconstruction ◽  
Cytochrome Oxidase Subunit ◽  
Species Determination ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Histone 3 ◽  
Two New Species ◽  
Nuclear Histone ◽  
Mitochondrial Cytochrome Oxidase

The Balkan Peninsula is inhabited by the worldwide most diverse subterranean gastropod fauna. This fauna is still poorly studied, since its habitats are not easily accessible, and its sampled populations are mostly not rich in specimens’ numbers. Often only empty shells are known, but the shell is hardly useful, not only in phylogeny reconstruction, but even in species determination. The exclusively obligatory subterranean family Moitessieriidae is especially poorly studied. Representatives of the genus Paladilhiopsis Pavlović, 1913 (Moitessieriidae) collected at three localities, distributed in Croatia and Bosnia & Herzegovina, were studied. The pigmentation of their shells and soft parts, as well as the female and male reproductive organs in one taxon, are presented. The partial sequences of the molecular markers mitochondrial cytochrome oxidase subunit I (COI) and nuclear histone 3 (H3) were used to infer their systematic status and phylogenetic relationships. Two species new to science are described. For one of them, also studied anatomically, 15 specimens were sequenced for COI, and all show the same haplotype.

scholarly journals A new species of Kerkia Radoman, 1978 (Caenogastropoda, Hydrobiidae) from Bosnia and Herzegovina

ZooKeys ◽  
2020 ◽  
Vol 973 ◽  
pp. 17-33
Author(s):  
Sebastian Hofman ◽  
Aleksandra Rysiewska ◽  
Artur Osikowski ◽  
Andrzej Falniowski
Keyword(s):  
New Species ◽  
Bosnia And Herzegovina ◽  
Histone H3 ◽  
Reproductive Organs ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Nuclear Histone ◽  
Mitochondrial Cytochrome Oxidase ◽  
A New Species ◽  
Female Reproductive Organs

A new species of Kerkia, K. briani Rysiewska & Osikowski, sp. nov. is described from the spring Polički Studenac Vrelo (Crkvina), adjacent to the Trebišnjica River (Bosnia and Herzegovina) collected with Bou-Rouch technique, pumped from an interstitial habitat 50 cm below the bottom of the spring. The shell, female reproductive organs, and the penis are described and illustrated. Mitochondrial cytochrome oxidase subunit I (COI) and nuclear histone H3 partial sequences confirm the distinctness of the new species, and molecularly based phylogenetic relationships of Kerkia are briefly presented.

scholarly journals The Naticidae (Mollusca: Gastropoda) of Giglio Island (Tuscany, Italy): Shell characters, live animals, and a molecular analysis of egg masses

Zootaxa ◽  
2008 ◽  
Vol 1770 (1) ◽  
pp. 1 ◽  
Author(s):  
THOMAS HUELSKEN ◽  
CARINA MAREK ◽  
STEFAN SCHREIBER ◽  
IRIS SCHMIDT ◽  
MICHAEL HOLLMANN
Keyword(s):  
Molecular Analysis ◽  
18S Rrna ◽  
Mitochondrial Cytochrome ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Egg Masses ◽  
Histone 3 ◽  
Giglio Island ◽  
Mitochondrial Cytochrome Oxidase ◽  
Systematic Collection

We investigated the occurence of members of the predatory caenogastropod family Naticidae in the littoral of the island of Giglio, Tuscany, Italy. We recorded a total of 8 species, all but one represented by both empty shells and living specimens. As most studies of Mediterranean naticids are based solely on empty shells, we here provide images of living animals for 7 out of the 8 species encountered; for several of these species this is the first photographic documentation of the animal. Our survey included a systematic collection of egg masses (”sand collars”) which were hatched in the laboratory. The larvae obtained as well as the sand collars themselves were used for molecular analysis of the species based on gene fragments of mitochondrial cytochrome oxidase subunit I (COI), histone 3 (H3), the mitochondrial 16S rRNA (16S), and 18S rRNA (18S). We show that such molecular analysis allows the confirmation of the identity of naticid species without having access to adult specimens or shells. This approach identified one additional naticid species for which no adult specimens or shells were found. Additionally, our molecular analysis allows consideration of naticid phylogeny.

scholarly journals New data on the valvatiform-shelled Hydrobiidae (Caenogastropoda, Truncatelloidea) from southern Greece

ZooKeys ◽  
2021 ◽  
Vol 1062 ◽  
pp. 31-47
Author(s):  
Sebastian Hofman ◽  
Jozef Grego ◽  
Zoltán Fehér ◽  
Zoltán Péter Erőss ◽  
Aleksandra Rysiewska ◽  
...  
Keyword(s):  
New Genus ◽  
Reproductive Organs ◽  
Single Specimen ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Geographic Ranges ◽  
New Localities ◽  
Mitochondrial Cytochrome Oxidase ◽  
Southern Greece ◽  
Female Reproductive Organs

The minute valvatiform-shelled Hydrobiidae are less studied than other hydrobiid gastropods. In this paper, new data on these snails are presented, which have been collected at twelve springs in southern Greece: one in Boeotia, one on Evvoia Island, and ten on the Peloponnese Peninsula. Mitochondrial cytochrome oxidase subunit I (COI) and nuclear histone (H3) have been used to confirm the determinations and infer the relationships of the studied gastropods. They represent the genera Daphniola, Graecoarganiella and Isimerope. New localities, expanding the known geographic ranges, have been presented for Daphniola hadei and Daphniola louisi. A species of Daphniola found at two localities has been identified as a species new to science, and its description, including the shell, penis, and female reproductive organs is given. Possible relationships between Graecoarganiella and Isimerope are discussed; their representatives are possibly new species. At one locality a single specimen likely represents a new genus: it was found to be most closely related with Islamia, but genetically (p-distance) too distant to be congeneric with Islamia.

Vappolotes, a new genus of coelotine spiders (Araneae, Agelenidae) from Guizhou, China

Zootaxa ◽  
2019 ◽  
Vol 4701 (5) ◽  
pp. 434-442
Author(s):  
BING LI ◽  
ZHE ZHAO ◽  
YUANXUE CHEN ◽  
ZHIYAN WU ◽  
SHUQIANG LI
Keyword(s):  
New Species ◽  
New Genus ◽  
Molecular Level ◽  
Southern China ◽  
Mitochondrial Cytochrome ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Two New Species ◽  
Partial Fragment ◽  
Mitochondrial Cytochrome Oxidase

A new genus of the subfamily Coelotinae F.O. Pickard-Cambridge, 1893, Vappolotes Zhao et S. Li gen. n., with two new species, V. ganlongensis Zhao et S. Li sp. n. (♂♀) and V. jianpingensis Zhao et S. Li sp. n. (♀), is described. The genus is restricted to southern China (Guizhou). Its relationship to other coelotine genera is discussed. A partial fragment of the mitochondrial cytochrome oxidase subunit I of both species were obtained to aid species identification at the molecular level. 

scholarly journals Crenobiont, stygophile and stygobiont molluscs in the hydrographic area of the Trebišnjica River Basin

ZooKeys ◽  
2021 ◽  
Vol 1047 ◽  
pp. 61-89
Author(s):  
Andrzej Falniowski ◽  
Brian Lewarne ◽  
Aleksandra Rysiewska ◽  
Artur Osikowski ◽  
Sebastian Hofman
Keyword(s):  
New Genus ◽  
Histone H3 ◽  
Reproductive Organs ◽  
Karst Area ◽  
New Genus And Species ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Galba Truncatula ◽  
Mitochondrial Cytochrome Oxidase ◽  
Female Reproductive Organs

In the paper the crenobiont, stygophile and stygobiont malacofauna of the karst area of Popovo Polje around Trebinje (Eastern Herzegovina, BiH) is presented. The materials were collected from springs, caves and interstitial habitats (with a Bou-Rouch pump) at 23 localities. The following species were found: Pisidium cf. personatum A.W. Malm, 1855, Theodoxus callosus (Deshayes, 1833), Sadleriana fluminensis (Küster, 1852), Radomaniola curta (Küster, 1852), Radomaniola cf. bosniaca (Radoman, 1973), Kerkia briani Rysiewska & Osikowski, 2020, Montenegrospeum bogici (Pešić & Glöer, 2012), Litthabitella chilodia (Westerlund, 1886), Travunijana vruljakensis Grego & Glöer, 2019, a new genus and species of the Sadlerianinae, Emmericia ventricosa Brusina, 1870, Iglica cf. absoloni (A.J. Wagner, 1914), Plagigeyeria tribunicae Schütt, 1963, Paladilhiopsis arion Rysiewska & Osikowski, 2021, Valvata montenegrina Glöer & Pešić, 2008, Radix labiata (Rossmässler, 1835), Galba truncatula (O. F. Müller, 1774), Ancylus recurvus Martens, 1783, Ancylus sp. and the amphibiotic Succinea cf. putris (Linnaeus, 1758). The redescription of the genus Travunijana Grego & Glöer, 2019, applying the characteristics of shell, female reproductive organs and penis, is also presented. The new genus and species are described, based on the shell, penis, radula and fragmentary data on the female reproductive organs. For all species, the mitochondrial cytochrome oxidase subunit I (COI) is applied to confirm the determination; in the case of Travunijana and the new genus, the nuclear histone H3 locus is also used, in order to infer both their distinctiveness and phylogenetic relationships.

scholarly journals Exploring Monacha cantiana (Montagu, 1803) phylogeography: cryptic lineages and new insights into the origin of the English populations (Eupulmonata, Stylommatophora, Hygromiidae)

ZooKeys ◽  
2018 ◽  
Vol 765 ◽  
pp. 1-41 ◽  
Author(s):  
Joanna R. Pieńkowska ◽  
Giuseppe Manganelli ◽  
Folco Giusti ◽  
Alessandro Hallgass ◽  
Andrzej Lesicki
Keyword(s):  
Morphological Analysis ◽  
Genetic Distances ◽  
Northern Italy ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Histone 3 ◽  
Italian Origin ◽  
The Uk ◽  
Distinct Taxon ◽  
Mitochondrial Cytochrome Oxidase

Molecular analysis of nucleotide sequences of mitochondrial cytochrome oxidase subunit 1 (COI) and 16S ribosomal DNA (16SrDNA) as well as nuclear histone 3 (H3) and internal transcribed spacer 2 of rDNA (ITS2) gene fragments together with morphological analysis of shell and genitalia features showed that English, French and Italian populations usually assigned to Monachacantiana consist of four distinct lineages (CAN-1, CAN-2, CAN-3, CAN-4). One of these lineages (CAN-1) included most of the UK (five sites) and Italian (five sites) populations examined. Three other lineages represented populations from two sites in northern Italy (CAN-2), three sites in northern Italy and Austria (CAN-3), and two sites in south-eastern France (CAN-4). The taxonomic and nomenclatural setting is only currently available for lineages CAN-1 and CAN-4; a definitive frame for the other two requires much more research. The lineage CAN-1 corresponds to the true M.cantiana (Montagu, 1803) because it is the only one that includes topotypical English populations. The relationships and genetic distances support the hypothesis of the Italian origin of this lineage which was probably introduced to England by the Romans. The lineage CAN-4 is attributed to M.cemenelea (Risso, 1826), for which a neotype has been designated and deposited. Its diagnostic sequences of COI, 16SrDNA, H3 and ITS2 genes have also been deposited in GenBank. Molecular and morphological (shell and genitalia) features showed that M.parumcincta (Rossmässler, 1834) is a distinct taxon from the M.cantiana lineages.

scholarly journals Two new freshwater crab species of the genus Nanhaipotamon Bott, 1968 (Crustacea, Decapoda, Potamidae) from Huizhou, Guangdong Province, southern China

Zootaxa ◽  
2021 ◽  
Vol 5026 (2) ◽  
pp. 221-238
Author(s):  
CHAO HUANG ◽  
SIYING MAO ◽  
HSI-TE SHIH
Keyword(s):  
New Species ◽  
Close Relationships ◽  
Southern China ◽  
Guangdong Province ◽  
Crab Species ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
The Third ◽  
Two New Species ◽  
Mitochondrial Cytochrome Oxidase

Two morphologically distinct new species of Nanhaipotamon Bott, 1968, are described from Huizhou, Guangdong Province, southern China. The smooth carapace and lack of flagellum on the third maxilliped exopod immediately separate N. incendium n. sp. from all other known Nanhaipotamon. It is also the first species of this genus to be recorded from a relatively high altitude of 700 m a.s.l. Nanhaipotamon aureomarginatum n. sp. is externally much more typical of species of its genus but can be distinguished from congeners by its unique male first gonopod. The two new species are more or less sympatric with a morphological disparate congener, which we tentatively assign as N. aff. aculatum Dai, 1997 and N. aff. hongkongense (Shen, 1940), respectively, but with different habitats. A genetic analysis using the mitochondrial cytochrome oxidase subunit I shows that while the two new species are sufficiently distinct from other known species of Nanhaipotamon, they have extraordinarily close relationships with their respective sympatric congeners, which calls for further investigation. The ecology of these two new species are also noted.  

First rearing record and DNA sequence of Chromatomyia aizoon (Hering, 1932) (Diptera: Agromyzidae) associated with Saxifraga paniculata Miller from the Czech Republic

2020 ◽  
Vol 69 (3) ◽  
pp. 283-288
Author(s):  
Miloš Černý ◽  
Jiří Kocián ◽  
Jan Ševčík
Keyword(s):  
Czech Republic ◽  
Cytochrome Oxidase ◽  
Dna Sequence ◽  
Partial Sequence ◽  
Mitochondrial Cytochrome ◽  
Cytochrome Oxidase Subunit ◽  
Cytochrome Oxidase Subunit I ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
The Czech Republic ◽  
Mitochondrial Cytochrome Oxidase

Abstract A male of Chromatomyia aizoon (Hering, 1932) (Diptera: Agromyzidae) was reared from a leaf mine at Saxifraga paniculata collected in Štramberk (Northern Moravia, Czech Republic). Partial sequence of the mitochondrial cytochrome oxidase subunit I (COI barcode region, 658 bp) is also provided.

Morphology and molecular phylogeny of ornamental freshwater prawns of the genus Macrobrachium (Decapoda, Caridea, Palaemonidae) from China with the description of a new species

Crustaceana ◽  
2021 ◽  
Vol 94 (10) ◽  
pp. 1201-1220
Author(s):  
Qinghua Chen ◽  
Wenjian Chen ◽  
Yuanwei Hu ◽  
Ka Yan Ma ◽  
Zhaoliang Guo
Keyword(s):  
New Species ◽  
Colour Pattern ◽  
Molecular Evidence ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Closely Related Species ◽  
Distribution Maps ◽  
Mitochondrial Cytochrome Oxidase ◽  
A New Species

Abstract This study dealt with three species of ornamental palaemonid freshwater prawns of the genus Macrobrachium, based on morphological and molecular analysis. Macrobrachium pentazona He, Gao & Guo, 2009; M. laevis Zheng, Chen & Guo, 2019; and M. bilineare sp. nov. are distinguishable from closely related species by segmental ratios, spination of the second pereiopods and the slender scaphocerite. Macrobrachium bilineare sp. nov. can easily be recognized in the field by its bright colour pattern. Molecular evidence of mitochondrial cytochrome oxidase subunit I (COI), also supports the characterization of this new species, raising the total number of Macrobrachium spp. known from China to 41. Detailed description, illustrations, colour photographs, habitat information, distribution maps and features of conservation significance are also briefly discussed.

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