scholarly journals Exploring Monacha cantiana (Montagu, 1803) phylogeography: cryptic lineages and new insights into the origin of the English populations (Eupulmonata, Stylommatophora, Hygromiidae)

ZooKeys ◽  
2018 ◽  
Vol 765 ◽  
pp. 1-41 ◽  
Author(s):  
Joanna R. Pieńkowska ◽  
Giuseppe Manganelli ◽  
Folco Giusti ◽  
Alessandro Hallgass ◽  
Andrzej Lesicki
Keyword(s):  
Morphological Analysis ◽  
Genetic Distances ◽  
Northern Italy ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Histone 3 ◽  
Italian Origin ◽  
The Uk ◽  
Distinct Taxon ◽  
Mitochondrial Cytochrome Oxidase

Molecular analysis of nucleotide sequences of mitochondrial cytochrome oxidase subunit 1 (COI) and 16S ribosomal DNA (16SrDNA) as well as nuclear histone 3 (H3) and internal transcribed spacer 2 of rDNA (ITS2) gene fragments together with morphological analysis of shell and genitalia features showed that English, French and Italian populations usually assigned to Monachacantiana consist of four distinct lineages (CAN-1, CAN-2, CAN-3, CAN-4). One of these lineages (CAN-1) included most of the UK (five sites) and Italian (five sites) populations examined. Three other lineages represented populations from two sites in northern Italy (CAN-2), three sites in northern Italy and Austria (CAN-3), and two sites in south-eastern France (CAN-4). The taxonomic and nomenclatural setting is only currently available for lineages CAN-1 and CAN-4; a definitive frame for the other two requires much more research. The lineage CAN-1 corresponds to the true M.cantiana (Montagu, 1803) because it is the only one that includes topotypical English populations. The relationships and genetic distances support the hypothesis of the Italian origin of this lineage which was probably introduced to England by the Romans. The lineage CAN-4 is attributed to M.cemenelea (Risso, 1826), for which a neotype has been designated and deposited. Its diagnostic sequences of COI, 16SrDNA, H3 and ITS2 genes have also been deposited in GenBank. Molecular and morphological (shell and genitalia) features showed that M.parumcincta (Rossmässler, 1834) is a distinct taxon from the M.cantiana lineages.

scholarly journals Two new species of the Balkan genus Paladilhiopsis Pavlović, 1913 (Caenogastropoda, Moitessieriidae)

ZooKeys ◽  
2021 ◽  
Vol 1046 ◽  
pp. 157-176
Author(s):  
Sebastian Hofman ◽  
Jozef Grego ◽  
Aleksandra Rysiewska ◽  
Artur Osikowski ◽  
Andrzej Falniowski
Keyword(s):  
Balkan Peninsula ◽  
Reproductive Organs ◽  
Phylogeny Reconstruction ◽  
Cytochrome Oxidase Subunit ◽  
Species Determination ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Histone 3 ◽  
Two New Species ◽  
Nuclear Histone ◽  
Mitochondrial Cytochrome Oxidase

The Balkan Peninsula is inhabited by the worldwide most diverse subterranean gastropod fauna. This fauna is still poorly studied, since its habitats are not easily accessible, and its sampled populations are mostly not rich in specimens’ numbers. Often only empty shells are known, but the shell is hardly useful, not only in phylogeny reconstruction, but even in species determination. The exclusively obligatory subterranean family Moitessieriidae is especially poorly studied. Representatives of the genus Paladilhiopsis Pavlović, 1913 (Moitessieriidae) collected at three localities, distributed in Croatia and Bosnia & Herzegovina, were studied. The pigmentation of their shells and soft parts, as well as the female and male reproductive organs in one taxon, are presented. The partial sequences of the molecular markers mitochondrial cytochrome oxidase subunit I (COI) and nuclear histone 3 (H3) were used to infer their systematic status and phylogenetic relationships. Two species new to science are described. For one of them, also studied anatomically, 15 specimens were sequenced for COI, and all show the same haplotype.

scholarly journals The Naticidae (Mollusca: Gastropoda) of Giglio Island (Tuscany, Italy): Shell characters, live animals, and a molecular analysis of egg masses

Zootaxa ◽  
2008 ◽  
Vol 1770 (1) ◽  
pp. 1 ◽  
Author(s):  
THOMAS HUELSKEN ◽  
CARINA MAREK ◽  
STEFAN SCHREIBER ◽  
IRIS SCHMIDT ◽  
MICHAEL HOLLMANN
Keyword(s):  
Molecular Analysis ◽  
18S Rrna ◽  
Mitochondrial Cytochrome ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Egg Masses ◽  
Histone 3 ◽  
Giglio Island ◽  
Mitochondrial Cytochrome Oxidase ◽  
Systematic Collection

We investigated the occurence of members of the predatory caenogastropod family Naticidae in the littoral of the island of Giglio, Tuscany, Italy. We recorded a total of 8 species, all but one represented by both empty shells and living specimens. As most studies of Mediterranean naticids are based solely on empty shells, we here provide images of living animals for 7 out of the 8 species encountered; for several of these species this is the first photographic documentation of the animal. Our survey included a systematic collection of egg masses (”sand collars”) which were hatched in the laboratory. The larvae obtained as well as the sand collars themselves were used for molecular analysis of the species based on gene fragments of mitochondrial cytochrome oxidase subunit I (COI), histone 3 (H3), the mitochondrial 16S rRNA (16S), and 18S rRNA (18S). We show that such molecular analysis allows the confirmation of the identity of naticid species without having access to adult specimens or shells. This approach identified one additional naticid species for which no adult specimens or shells were found. Additionally, our molecular analysis allows consideration of naticid phylogeny.

scholarly journals The Analysis of Morphological and Genetic Characteristics of Yellowstripe Scad from Muara Baru Modern Fish Market in North Jakarta

2020 ◽  
Vol 12 (2) ◽  
pp. 308
Author(s):  
Rahmat Sawalman ◽  
Hawis Madduppa
Keyword(s):  
Dna Barcoding ◽  
Molecular Analysis ◽  
Fish Species ◽  
Morphological Analysis ◽  
Coi Gene ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Fish Market ◽  
Commercially Important ◽  
Mitochondrial Cytochrome Oxidase

HighlightIdentification and morphological analysis of Selaroides leptolepis fish.Molecular analysis using the DNA Barcoding method with the COI gene (mitochondrial cytochrome oxidase subunit I).The number of base pairs in S. leptolepis from the sequencing results was 675bp.The morphological and molecular similarities of Selaroides leptolepis fishAbstractYellowstripe scad is one of the commercially important fish from the Carangidae family, which is marketed at Muara Baru Modern Fish Market, North Jakarta. In- formation regarding the presence of Selaroides leptolepis fish in the waters allows for effective conservation, and management of marine resources. A morphological identification of fish species is still considered inaccurate, so the molecular anal- ysis is necessary. This study aims to identify commercially important fish species deriving from Muara Baru Modern Fish Market, North Jakarta, employing mor- phological, and molecular analysis. A total of 30 specimens were collected from the field. The morphological analysis utilized a visual identification method, and morphometric measurement, while molecular analysis with DNA barcoding em- ployed the mitochondrial cytochrome oxidase subunit I (COI) gene. The results of the morphological analysis indicated that the fish species were yellowstripe scad (S. leptolepis). It also followed the results of molecular analysis of DNA barcoding that the fish was a S. leptolepis species. Therefore, the combination of morphological and genetic analysis has succeeded in identifying the fish species of S. leptolepis.

First rearing record and DNA sequence of Chromatomyia aizoon (Hering, 1932) (Diptera: Agromyzidae) associated with Saxifraga paniculata Miller from the Czech Republic

2020 ◽  
Vol 69 (3) ◽  
pp. 283-288
Author(s):  
Miloš Černý ◽  
Jiří Kocián ◽  
Jan Ševčík
Keyword(s):  
Czech Republic ◽  
Cytochrome Oxidase ◽  
Dna Sequence ◽  
Partial Sequence ◽  
Mitochondrial Cytochrome ◽  
Cytochrome Oxidase Subunit ◽  
Cytochrome Oxidase Subunit I ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
The Czech Republic ◽  
Mitochondrial Cytochrome Oxidase

Abstract A male of Chromatomyia aizoon (Hering, 1932) (Diptera: Agromyzidae) was reared from a leaf mine at Saxifraga paniculata collected in Štramberk (Northern Moravia, Czech Republic). Partial sequence of the mitochondrial cytochrome oxidase subunit I (COI barcode region, 658 bp) is also provided.

Morphology and molecular phylogeny of ornamental freshwater prawns of the genus Macrobrachium (Decapoda, Caridea, Palaemonidae) from China with the description of a new species

Crustaceana ◽  
2021 ◽  
Vol 94 (10) ◽  
pp. 1201-1220
Author(s):  
Qinghua Chen ◽  
Wenjian Chen ◽  
Yuanwei Hu ◽  
Ka Yan Ma ◽  
Zhaoliang Guo
Keyword(s):  
New Species ◽  
Colour Pattern ◽  
Molecular Evidence ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Closely Related Species ◽  
Distribution Maps ◽  
Mitochondrial Cytochrome Oxidase ◽  
A New Species

Abstract This study dealt with three species of ornamental palaemonid freshwater prawns of the genus Macrobrachium, based on morphological and molecular analysis. Macrobrachium pentazona He, Gao & Guo, 2009; M. laevis Zheng, Chen & Guo, 2019; and M. bilineare sp. nov. are distinguishable from closely related species by segmental ratios, spination of the second pereiopods and the slender scaphocerite. Macrobrachium bilineare sp. nov. can easily be recognized in the field by its bright colour pattern. Molecular evidence of mitochondrial cytochrome oxidase subunit I (COI), also supports the characterization of this new species, raising the total number of Macrobrachium spp. known from China to 41. Detailed description, illustrations, colour photographs, habitat information, distribution maps and features of conservation significance are also briefly discussed.

scholarly journals Epimeria schiaparelli sp. nov., an amphipod crustacean (family Epimeriidae) from the Ross Sea, Antarctica, with molecular characterisation of the species complex  

Zootaxa ◽  
2007 ◽  
Vol 1402 (1) ◽  
pp. 23 ◽  
Author(s):  
ANNE-NINA LÖRZ ◽  
ELIZABETH W. MAAS ◽  
KATRIN LINSE ◽  
GRAHAM D. FENWICK
Keyword(s):  
Species Complex ◽  
Ross Sea ◽  
Similar Species ◽  
Molecular Characterisation ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Antarctic Species ◽  
Amphipod Crustacean ◽  
Colour Patterns ◽  
Mitochondrial Cytochrome Oxidase

Epimeria schiaparelli sp. nov. from the Ross Sea, Antarctica, is described in detail. The new species occurs on the shelf in 130–350 m depth. Epimeria schiaparelli can be distinguished from the most similar species, E. similis Chevreux, 1912 and E. macrodonta Walker, 1906 by a relatively short rostrum and a short second pereonite amongst other characters. Two distinct colour patterns are reported. Partial gene sequences of the mitochondrial cytochrome oxidase subunit I (COI) from 11 specimens of E. schiaparelli confirm that this species is new to science and closely related to E. similis, E. macrodonta and E. reoproi. The recent and historical separation of this Antarctic species is discussed. The syntypes of E. macrodonta consist of two species, so a lectotype is here designated.

Redefinition of Cornexcisia Fan & Kano, 2000 (Diptera: Sarcophagidae), with the first description of the female of Sarcophaga kurahashii (Shinonaga & Tumrasvin, 1979)

Zootaxa ◽  
2019 ◽  
Vol 4668 (3) ◽  
pp. 410-420
Author(s):  
CHAO WANG ◽  
YUNYUN GAO ◽  
THOMAS PAPE ◽  
DONG ZHANG
Keyword(s):  
Cytochrome Oxidase ◽  
Coi Gene ◽  
Mitochondrial Cytochrome ◽  
Gene Sequences ◽  
Cytochrome Oxidase Subunit ◽  
Cytochrome Oxidase Subunit I ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Senior Synonym ◽  
First Time ◽  
Mitochondrial Cytochrome Oxidase

Sarcophaga Meigen, 1826 is proposed as a senior synonym of Cornexcisia Fan & Kano, 2000, syn. nov. and Fanzideia Xue, Verves & Du, 2011, syn. nov. Cornexcisia Fan & Kano, 2000, stat. rev. is given status as a subgenus and is considered a senior synonym of Fanzideia Xue, Verves & Du, 2011, syn. nov. at the subgeneric level. Cornexcisia is argued to contain S. (Cornexcisia) longicornuta (Fan & Kano, 2000), comb. nov., S. (C.) cygnocerca (Xue, Verves & Du, 2011), comb. nov., S. (C.) kurahashii (Shinonaga & Tumrasvin, 1979), subgen. comb. nov. (from Phallosphaera Rohdendorf) and S. (C.) suthep Pape & Bänziger, 2003, subgen. comb. nov. (from Rosellea Rohdendorf). Sarcophaga (C.) kurahashii is newly recorded from China (Yunnan), the male is redescribed and the female is described for the first time, supported by photographs, illustrations and mitochondrial cytochrome oxidase subunit I (COI) gene sequences. Species of Cornexcisia share an exceptionally long postpedicel in the female and the following apomorphic distiphallic appendages in the male: juxta ventro-proximally with an apically divided arm with cuticular pile, and lateral styli bifurcated from the base with each branch elongate, gently curved and slightly expanded apically. A key to the species of Cornexcisia is provided. 

Morphological identification and molecular validation of anchovies (Engraulidae) in the Persian Gulf and Oman Sea

Zootaxa ◽  
2020 ◽  
Vol 4742 (2) ◽  
pp. 375-391
Author(s):  
MAHBOOBEH AFRAND ◽  
IMAN SOURINEJAD ◽  
SEYED ABOLHASSAN SHAHZADEH FAZELI ◽  
ARASH AKBARZADEH ◽  
LALEH PARSA YEGANEH ◽  
...  
Keyword(s):  
Persian Gulf ◽  
Morphological Characteristics ◽  
Morphological Differentiation ◽  
Genetic Distances ◽  
Morphological Identification ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Closely Related Species ◽  
Oman Sea ◽  
The Persian Gulf ◽  
Mitochondrial Cytochrome Oxidase

Validation of species using independent lines of evidence is sometimes desirable when their identification using only one approach is difficult or questionable. The identification of anchovies (Engraulidae) are often challenging based on morphology because closely related species exhibit only slight morphological differentiation. This study utilized morphological characteristics and DNA barcodes for identification and validation of anchovies in the Persian Gulf and Oman Sea. Based on morphology, we identified eight species: Thryssa hamiltonii, T. setirostris, T. vitrirostris, T. whiteheadi, T. dussumieri, Encrasicholina punctifer, E. pseudoheteroloba and Stolephorus indicus. A 658 bp region of mitochondrial cytochrome oxidase subunit I (COI) was generated for 53 specimens from these eight species. From these sequences, we built a Maximum Likelihood phylogenetic tree. In this tree, each species forms a monophyletic group confirming our initial morphological identification. In addition, we provided (and registered in GenBank) the first barcode sequences for T. whiteheadi, an endemic species of this region. Interspecies genetic distances were comprised between 0.168 to 0.275. The largest genetic distance was found between T. vitrirostris and S. indicus and the smallest between T. dussumieri and T. whiteheadi. This study successfully identified eight species of anchovies in the Persian Gulf and Oman Sea based on both morphological and molecular characters. 

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