scholarly journals In silico comparative proteomic analysis of enzymes involved in fatty acid biosynthesis in castor bean (Ricinus communis) and soybean (Glycine max)

2019 ◽  
pp. 1-26
Author(s):  
Qudsia YOUSAFI ◽  
Hafsa Ahmad ALI ◽  
Hamid RASHID ◽  
Muhammad Saad KHAN
Keyword(s):  
Glycine Max ◽  
Fatty Acid ◽  
Sequence Alignment ◽  
Seed Oil ◽  
Fatty Acid Biosynthesis ◽  
Ricinus Communis ◽  
Acid Biosynthesis ◽  
Pairwise Sequence Alignment ◽  
Plant Seed ◽  
Plant Seed Oil

Plant oils are very important for domestic and industrial use. Biodiesel can be obtained from plant seed oil. Biodiesel is currently popular and in demand due to the high cost of petroleum and to avoid pollution. It is time to increase plant seed oil production and conduct research to find ways of enhancing its production. We studied two species of oil seed plants, i.e. Ricinus communis and Glycine max, with varying amounts of oil content. Proteins from six categories of enzymes involved in fatty acid biosynthesis were selected for study. The 3D structures were predicted using different structure prediction tools. The structures were validated and selected on the basis of quality factors. The pairs of proteins were compared by pairwise sequence alignment using Clustal W and structural superposition by Chimera Matchmaker. The physiochemical properties were studied by PROTPARAM. In R. communis, eighteen structures were selected from I Tasser, thirteen from Swiss Model, and two from Raptorx. In G. max, twenty structures were selected from I Tasser, nine from Swiss Model, and four from Raptorx. The highest percent identity in pairwise sequence alignment was observed between the two species for biotin carboxylase. Biotin carrier was least identical between these two species. Monogalactosyldiacylglycerol desaturase (FAD5) showed the highest percentage of structural identity between the two species while ER phosphatidate phosphate was least identical. Eight proteins in both species had an instability index below 40. Eight proteins in R. communis and five in G. max were acidic in nature. Fourteen proteins in R. communis and seventeen in G. max were hydrophobic. The aliphatic index of all proteins was above 50 with which conferes good thermal stability.

Metabolic engineering of fatty acid biosynthesis in Indian mustard (Brassica juncea) improves nutritional quality of seed oil

2007 ◽  
Vol 1 (4) ◽  
pp. 185-197 ◽  
Author(s):  
Saheli Sinha ◽  
Jyoti K. Jha ◽  
Mrinal K. Maiti ◽  
Asitava Basu ◽  
Ujjal K. Mukhopadhyay ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Metabolic Engineering ◽  
Brassica Juncea ◽  
Nutritional Quality ◽  
Seed Oil ◽  
Fatty Acid Biosynthesis ◽  
Indian Mustard ◽  
Acid Biosynthesis

scholarly journals Improving soybean seed oil without poor agronomics

2020 ◽  
Vol 71 (22) ◽  
pp. 6857-6860
Author(s):  
Miguel Alfonso
Keyword(s):  
Fatty Acid ◽  
Unsaturated Fatty Acid ◽  
Seed Oil ◽  
Fatty Acid Biosynthesis ◽  
Soybean Seed ◽  
Acid Biosynthesis ◽  
Experimental Botany ◽  
Tilling By Sequencing

This article comments on: Lakhssassi N, Zhou Z, Liu S, Piya S, Cullen MA, El Baze A, Knizia D, Patil GB, Badad O, Embaby MG, Meksem J, Lakhssassi A, Ghazaleh A, Hewezi T, Meksem K. 2020. Soybean TILLING-by-sequencing+ reveals the role of novel GmSACPD members in unsaturated fatty acid biosynthesis while maintaining healthy nodules. Journal of Experimental Botany 71, 6969–6987.

scholarly journals Genome-wide approaches delineate the additive, epistatic, and pleiotropic nature of variants controlling fatty acid composition in peanut (Arachis hypogaea L.).

2021 ◽  
Author(s):  
Paul I. Otyama ◽  
Kelly Chamberlin ◽  
Peggy Ozias-Akins ◽  
Michelle A. Graham ◽  
Ethalinda K.S. Cannon ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Acid Composition ◽  
Acid Content ◽  
Seed Oil ◽  
Fatty Acid Biosynthesis ◽  
Functional Enrichment ◽  
Acid Biosynthesis ◽  
Genome Wide

The fatty acid composition of seed oil is a major determinant of the flavor, shelf-life, and nutritional quality of peanuts. Major QTLs controlling high oil content, high oleic content, and low linoleic content have been characterized in several seed oil crop species. Here we employ genome-wide association approaches on a recently genotyped collection of 787 plant introduction accessions in the USDA peanut core collection, plus selected improved cultivars, to discover markers associated with the natural variation in fatty acid composition, and to explain the genetic control of fatty acid composition in seed oils. Overall, 251 single nucleotide polymorphisms (SNPs) had significant trait associations with the measured fatty acid components. Twelve SNPs were associated with two or three different traits. Of these loci with apparent pleiotropic effects, 10 were associated with both oleic (C18:1) and linoleic acid (C18:2) content at different positions in the genome. In all 10 cases, the favorable allele had an opposite effect - increasing and lowering the concentration, respectively, of oleic and linoleic acid. The other traits with pleiotropic variant control were palmitic (C16:0), behenic (C22:0), lignoceric (C24:0), gadoleic (C20:1), total saturated, and total unsaturated fatty acid content. One hundred (100) of the significantly associated SNPs were located within 1000 kbp of 55 genes with fatty acid biosynthesis functional annotations. These genes encoded, among others: ACCase carboxyl transferase subunits, and several fatty acid synthase II enzymes. With the exception of gadoleic (C20:1) and lignoceric (C24:0) acid content, which occur at relatively low abundance in cultivated peanut, all traits had significant SNP interactions exceeding a stringent Bonferroni threshold (α = 1%). We detected 7,682 pairwise SNP interactions affecting the relative abundance of fatty acid components in the seed oil. Of these, 627 SNP pairs had at least one SNP within 1000 kbp of a gene with fatty acid biosynthesis functional annotation. We evaluated 168 candidate genes underlying these SNP interactions. Functional enrichment and protein-to-protein interactions supported significant interactions (p-value < 1.0E-16) among the genes evaluated. These results show the complex nature of the biology and genes underlying the variation in seed oil fatty acid composition and contribute to an improved genotype-to-phenotype map for fatty acid variation in peanut seed oil.

Heat Stress Suppresses Brassica napus Seed Oil Accumulation by Inhibition of Photosynthesis and BnWRI1 Pathway

2019 ◽  
Vol 60 (7) ◽  
pp. 1457-1470 ◽  
Author(s):  
Ruizhi Huang ◽  
Zhihong Liu ◽  
Meiqing Xing ◽  
Yong Yang ◽  
Xuelong Wu ◽  
...  
Keyword(s):  
Heat Stress ◽  
Fatty Acid ◽  
Brassica Napus ◽  
Seed Oil ◽  
Fatty Acid Biosynthesis ◽  
De Novo ◽  
Sugar Content ◽  
Light Response ◽  
Acid Biosynthesis ◽  
Oil Accumulation

Abstract Heat stress during Brassica napus seed filling severely impairs yield and oil content. However, the mechanisms underlying heat-stress effects on B. napus seed photosynthesis and oil accumulation remain elusive. In this study, we showed that heat stress resulted in reduction of seed oil accumulation, whereas the seed sugar content was enhanced, which indicated that incorporation of carbohydrates into triacylglycerols was impaired. Photosynthesis and respiration rates, and the maximum quantum yield of photosystem II in developing seeds were inhibited by heat stress. Transcriptome analysis revealed that heat stress led to up-regulation of genes associated with high light response, providing evidence that photoinhibition was induced by heat stress. BnWRI1 and its downstream genes, including genes involved in de novo fatty acid biosynthesis pathway, were down-regulated by heat stress. Overexpression of BnWRI1 with a seed-specific promoter stabilized both oil accumulation and photosynthesis under the heat-stress condition, which suggested BnWRI1 plays an important role in mediating the effect of heat stress on fatty acid biosynthesis. A number of sugar transporter genes were inhibited by heat stress, resulting in defective integration of carbohydrates into triacylglycerols units. The results collectively demonstrated that disturbances of the seed photosynthesis machinery, impairment of carbohydrates incorporation into triacylglycerols and transcriptional deregulation of the BnWRI1 pathway by heat stress might be the major cause of decreased oil accumulation in the seed.

scholarly journals Genome-wide approaches delineate the additive, epistatic, and pleiotropic nature of variants controlling fatty acid composition in peanut (Arachis hypogaea L.)

2021 ◽  
Author(s):  
Paul I Otyama ◽  
Kelly Chamberlin ◽  
Peggy Ozias-Akins ◽  
Michelle A Graham ◽  
Ethalinda K S Cannon ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Acid Composition ◽  
Acid Content ◽  
Seed Oil ◽  
Fatty Acid Biosynthesis ◽  
Functional Enrichment ◽  
Acid Biosynthesis ◽  
Genome Wide

Abstract The fatty acid composition of seed oil is a major determinant of the flavor, shelf-life, and nutritional quality of peanuts. Major QTLs controlling high oil content, high oleic content, and low linoleic content have been characterized in several seed oil crop species. Here we employ genome-wide association approaches on a recently genotyped collection of 787 plant introduction accessions in the USDA peanut core collection, plus selected improved cultivars, to discover markers associated with the natural variation in fatty acid composition, and to explain the genetic control of fatty acid composition in seed oils. Overall, 251 single nucleotide polymorphisms (SNPs) had significant trait associations with the measured fatty acid components. Twelve SNPs were associated with two or three different traits. Of these loci with apparent pleiotropic effects, 10 were associated with both oleic (C18:1) and linoleic acid (C18:2) content at different positions in the genome. In all 10 cases, the favorable allele had an opposite effect—increasing and lowering the concentration, respectively, of oleic and linoleic acid. The other traits with pleiotropic variant control were palmitic (C16:0), behenic (C22:0), lignoceric (C24:0), gadoleic (C20:1), total saturated, and total unsaturated fatty acid content. One hundred (100) of the significantly associated SNPs were located within 1000 kbp of 55 genes with fatty acid biosynthesis functional annotations. These genes encoded, among others: ACCase carboxyl transferase subunits, and several fatty acid synthase II enzymes. With the exception of gadoleic (C20:1) and lignoceric (C24:0) acid content, which occur at relatively low abundance in cultivated peanut, all traits had significant SNP interactions exceeding a stringent Bonferroni threshold (α = 1%). We detected 7,682 pairwise SNP interactions affecting the relative abundance of fatty acid components in the seed oil. Of these, 627 SNP pairs had at least one SNP within 1000 kbp of a gene with fatty acid biosynthesis functional annotation. We evaluated 168 candidate genes underlying these SNP interactions. Functional enrichment and protein-to-protein interactions supported significant interactions (p-value &lt; 1.0E-16) among the genes evaluated. These results show the complex nature of the biology and genes underlying the variation in seed oil fatty acid composition and contribute to an improved genotype-to-phenotype map for fatty acid variation in peanut seed oil.

Inhibition of early steps of de novo fatty-acid biosynthesis by different xenobiotica

1991 ◽  
Vol 81 (2) ◽  
pp. 251-255
Author(s):  
Manfred Focke ◽  
Andrea Feld ◽  
Hartmut K. Lichtenthaler
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Biosynthesis ◽  
De Novo ◽  
Acid Biosynthesis

Development of 'S', 'N’ Heterocycles as Antimycobacterials Targeting Fatty Acid Biosynthesis

2019 ◽  
Vol 15 ◽  
Author(s):  
L. K. Dahiwade ◽  
S. R. Rochlani ◽  
P. B. Choudhari ◽  
R. P. Dhavale ◽  
H. N. Moreira
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Biosynthesis ◽  
Antimycobacterial Activity ◽  
Drug Candidate ◽  
Causative Organism ◽  
Present Communication ◽  
Acid Biosynthesis ◽  
Rational Development ◽  
After Cancer ◽  
Drug Resistant Strains

Background: Mycobacterium tuberculosis is a causative organism of tuberculosis, which is most deadly disease after cancer in a current decade. The development of multidrug and broadly drug- resistant strains making the tuberculosis problem more and more critical. In last 40 years, only one molecule is added to the treatment regimen. Generally, drug design and development programs are targeted proteins whose function is known to be essential to the bacterial cell. Objectives: Reported here are the development of 'S', 'N’ heterocycles as antimycobacterials targeting fatty acid biosynthesis. Material and Methods: In the present communication, rational development of anti-mycobacterial agent's targeting fatty acid biosynthesis has been done by integrating the pocket modelling and virtual analysis. Results: The identified potential 33 lead compounds were synthesized, characterized by physicochemical and spectroscopic methods like IR, NMR spectroscopy and further screened for antimycobacterial activity using isoniazid as standard. All the designed compounds have shown profound antimycobacterial activity. Conclusion: In this present communication, we found that 3c, 3f, 3l and 4k molecules had expressive desirable biological activity and specific interactions with fatty acids. Further optimization of these leads is necessary for the development of potential antimycobacterial drug candidate having less side effects.

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