Survival and metabolic activity of the GanedenBC30 strain of Bacillus coagulans in a dynamic in vitro model of the stomach and small intestine

2010 ◽  
Vol 1 (1) ◽  
pp. 31-36 ◽  
Author(s):  
A. Maathuis ◽  
D. Keller ◽  
S. Farmer
Keyword(s):  
Small Intestine ◽  
Milk Protein ◽  
Intestinal Tract ◽  
In Vitro Model ◽  
Bacillus Coagulans ◽  
Protein Digestion ◽  
Vitro Model ◽  
Gastro Intestinal Tract ◽  
Carbohydrate Digestion

We have investigated the survival and activity of GanedenBC30 during passage through the upper gastro-intestinal tract. GanedenBC30 was tested in a dynamic, validated, in vitro model of the stomach and small intestine (TIM-1) on survival and its potential to aid in digestion of milk protein, lactose and fructose. The survival of GanedenBC30 was high (70%), although germination of the spores was minimal (<10%) under the conditions tested. Survival of the strain in the presence of lactose and fructose was markedly lower (56-59%) than in the absence of the sugars. The amount of digested milk protein available for absorption was somewhat higher (+0.2 g) when GanedenBC30 was added to the milk. When GanedenBC30 was tested with lactose or fructose added to the meal, the cumulative amount of lactate produced was slightly higher (+0.12-0.18 mmol) compared to the GanedenBC30 alone. In conclusion, although the differences in survival of GanedenBC30 are small, these results show the potential of GanedenBC30 to aid in protein digestion and in the digestion of lactose and fructose. If a larger fraction of the Bacillus coagulans cells had germinated, the influence on protein and carbohydrate digestion would probably have been much greater. Importance of the findings: the potential of GanedenBC30 to aid in the digestion of lactose and fructose could be used to prevent occurrence of intestinal symptoms in individuals sensitive to these carbohydrates.

scholarly journals Bacillus coagulans GBI-30, 6086 improves amino acid absorption from milk protein

2020 ◽  
Vol 17 (1) ◽  
Author(s):  
Richard A. Stecker ◽  
Jessica M. Moon ◽  
Travis J. Russo ◽  
Kayla M. Ratliff ◽  
Petey W. Mumford ◽  
...  
Keyword(s):  
Amino Acids ◽  
Small Intestine ◽  
Amino Acid ◽  
Milk Protein ◽  
Bacillus Coagulans ◽  
Protein Concentrate ◽  
Protein Digestion ◽  
Milk Protein Concentrate ◽  
Mean Differences

Abstract Background Probiotic Bacillus coagulans GBI-30, 6086 (BC30) has been shown to increase protein digestion in an in vitro model of the stomach and small intestine. Once active in the small intestine after germination, BC30 aids the digestion of carbohydrates and proteins. The extent to which BC30 administration may impact protein digestion and amino acid appearance in humans after protein ingestion is currently unknown. This study examined the impact of adding BC30 to a 25-g dose of milk protein concentrate on post-prandial changes in blood amino acids concentrations. Methods 14 males and 16 females (n = 30, 26.4 ± 6.5 years; 172.3 ± 10.8 cm; 78.2 ± 14.8 kg; 22.6 ± 7.2% fat) completed two supplementation protocols that each spanned two weeks separated by a washout period that lasted three weeks. Participants were instructed to track their dietary intake and ingest a daily 25-g dose of milk protein concentrate with (MPCBC30) or without (MPC) the addition of BC30. Body composition and demographics were assessed upon arrival to the laboratory. Upon ingestion of their final assigned supplemental dose, blood samples were taken at 0 (baseline), 30, 60, 90, 120, 180, and 240 min post-consumption and analyzed for amino acid concentrations. Results Arginine (p = 0.03) and Isoleucine (p = 0.05) revealed greater area-under-the curve (AUC) in MPCBC30 group compared to MPC. In addition, Arginine (p = 0.02), Serine (p = 0.01), Ornithine (p = 0.02), Methionine (p = 0.04), Glutamic Acid (p = 0.01), Phenylalanine (p = 0.05), Isoleucine (p = 0.04), Tyrosine (p = 0.02), Essential Amino Acids (p = 0.02), and Total Amino Acids (p < 0.01) all revealed significantly greater concentration maximum (CMax) in MPCBC30 compared to MPC. Finally, time to reach CMax (TMax) was significantly faster for Glutamine (p < 0.01), Citrulline (p < 0.01), Threonine (p = 0.04), Alanine (p = 0.02) in MPCBC30 when compared to MPC. Greater mean differences between groups for AUC and CMax in women when compared to the mean differences in men were found for several amino acids. Conclusion In concert with previous in vitro evidence of improved protein digestion and amino acid appearance, these results reveal that adding BC30 to protein sources such as milk protein concentrate can improve AUC, CMax, and faster TMax. Follow-up research should examine differences between gender and explore how aging can impact these outcomes. Retrospectively registered on June 11, 2020 at ClinicalTrials.gov as NCT04427020.

scholarly journals Silicon dioxide nanoparticle exposure affects small intestine function in an in vitro model

2018 ◽  
Vol 12 (5) ◽  
pp. 485-508 ◽  
Author(s):  
Zhongyuan Guo ◽  
Nicole J. Martucci ◽  
Yizhong Liu ◽  
Eusoo Yoo ◽  
Elad Tako ◽  
...  
Keyword(s):  
Small Intestine ◽  
Silicon Dioxide ◽  
In Vitro Model ◽  
Nanoparticle Exposure ◽  
Vitro Model ◽  
Silicon Dioxide Nanoparticle

scholarly journals Quantification of Salmonella Survival and Infection in an In vitro Model of the Human Intestinal Tract as Proxy for Foodborne Pathogens

2017 ◽  
Vol 8 ◽  
Author(s):  
Lucas M. Wijnands ◽  
Peter F. M. Teunis ◽  
Angelina F. A. Kuijpers ◽  
Ellen H. M. Delfgou-Van Asch ◽  
Annemarie Pielaat
Keyword(s):  
Foodborne Pathogens ◽  
Intestinal Tract ◽  
In Vitro Model ◽  
Vitro Model ◽  
Salmonella Survival ◽  
Human Intestinal Tract

Differentiated CaCo-2 cells as an in-vitro model to evaluate de-novo apolipoprotein A-I production in the small intestine

2009 ◽  
Vol 21 (6) ◽  
pp. 642-649 ◽  
Author(s):  
Stefan P.J. Dullens ◽  
Ronald P. Mensink ◽  
Edwin C.M. Mariman ◽  
Jogchum Plat
Keyword(s):  
Small Intestine ◽  
In Vitro Model ◽  
De Novo ◽  
Apolipoprotein A ◽  
Vitro Model

scholarly journals Effects of functional pasta ingredients on different gut microbiota as revealed by TIM-2 in vitro model of the proximal colon

2019 ◽  
Vol 10 (3) ◽  
pp. 301-313
Author(s):  
A. Martina ◽  
G.E. Felis ◽  
M. Corradi ◽  
C. Maffeis ◽  
S. Torriani ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
In Vitro Model ◽  
Bacillus Coagulans ◽  
Proximal Colon ◽  
Gene Profiling ◽  
Rrna Gene ◽  
Dietary Interventions ◽  
Vitro Model ◽  
Microbial Groups

Diet-related modulation of gut microbiota and its metabolic activity represents an intriguing research context, particularly in the case of disorders related to imbalances in gut microbial communities. We here explored the effects of Bacillus coagulans GBI-30, 6086 (BC30), β-glucans, and innovative whole-grain pastas, with or without these functional ingredients, on gut microbiota from three groups of children, presenting different susceptibility to type 1 diabetes, by using the well-controlled TNO in vitro model of the proximal colon (TIM-2). Short- and branched-chain fatty acids production and microbiota composition were assessed by means of gas chromatography and 16S rRNA gene profiling, respectively. In most cases, in vitro dietary interventions caused microbiota-dependent modulations as a result of intergroup variability, but also specific changes in microbial groups were shared between the three microbiotas, highlighting specific diet-microbial taxa connections.

Sign in / Sign up

Export Citation Format

Share Document