Chicory root and inulin stimulate butyrate-producing bacterial communities in an in vitro model of the piglet’s gastro-intestinal tract

We have investigated the survival and activity of GanedenBC30 during passage through the upper gastro-intestinal tract. GanedenBC30 was tested in a dynamic, validated, in vitro model of the stomach and small intestine (TIM-1) on survival and its potential to aid in digestion of milk protein, lactose and fructose. The survival of GanedenBC30 was high (70%), although germination of the spores was minimal (<10%) under the conditions tested. Survival of the strain in the presence of lactose and fructose was markedly lower (56-59%) than in the absence of the sugars. The amount of digested milk protein available for absorption was somewhat higher (+0.2 g) when GanedenBC30 was added to the milk. When GanedenBC30 was tested with lactose or fructose added to the meal, the cumulative amount of lactate produced was slightly higher (+0.12-0.18 mmol) compared to the GanedenBC30 alone. In conclusion, although the differences in survival of GanedenBC30 are small, these results show the potential of GanedenBC30 to aid in protein digestion and in the digestion of lactose and fructose. If a larger fraction of the Bacillus coagulans cells had germinated, the influence on protein and carbohydrate digestion would probably have been much greater. Importance of the findings: the potential of GanedenBC30 to aid in the digestion of lactose and fructose could be used to prevent occurrence of intestinal symptoms in individuals sensitive to these carbohydrates.

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Quantification of Salmonella Survival and Infection in an In vitro Model of the Human Intestinal Tract as Proxy for Foodborne Pathogens

Frontiers in Microbiology ◽

10.3389/fmicb.2017.01139 ◽

2017 ◽

Vol 8 ◽

Cited By ~ 2

Author(s):

Lucas M. Wijnands ◽

Peter F. M. Teunis ◽

Angelina F. A. Kuijpers ◽

Ellen H. M. Delfgou-Van Asch ◽

Annemarie Pielaat

Keyword(s):

Foodborne Pathogens ◽

Intestinal Tract ◽

In Vitro Model ◽

Vitro Model ◽

Salmonella Survival ◽

Human Intestinal Tract

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The fate of chicory root pulp polysaccharides during fermentation in the TNO in vitro model of the colon (TIM-2)

Bioactive Carbohydrates and Dietary Fibre ◽

10.1016/j.bcdf.2014.06.007 ◽

2014 ◽

Vol 4 (1) ◽

pp. 48-57 ◽

Cited By ~ 9

Author(s):

Uttara S. Ramasamy ◽

Koen Venema ◽

Harry Gruppen ◽

Henk A. Schols

Keyword(s):

In Vitro Model ◽

Chicory Root ◽

Vitro Model

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Symbiolite formation: a powerful in vitro model to untangle the role of bacterial communities in the photosynthesis-induced formation of microbialites

The ISME Journal ◽

10.1038/s41396-020-0629-z ◽

2020 ◽

Vol 14 (6) ◽

pp. 1533-1546

Author(s):

Matthew R. Nitschke ◽

Cátia Fidalgo ◽

João Simões ◽

Cláudio Brandão ◽

Artur Alves ◽

...

Keyword(s):

Bacterial Communities ◽

In Vitro Model ◽

Vitro Model

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Bifidobacterium longum D2 enhances microbial degradation of long-chain arabinoxylans in an in vitro model of the proximal colon

Beneficial Microbes ◽

10.3920/bm2015.0023 ◽

2015 ◽

Vol 6 (6) ◽

pp. 849-860 ◽

Cited By ~ 7

Author(s):

P. Truchado ◽

P. Van den Abbeele ◽

A. Rivière ◽

S. Possemiers ◽

L. De Vuyst ◽

...

Keyword(s):

Enzyme Activities ◽

Bacterial Communities ◽

In Vitro Model ◽

Bifidobacterium Longum ◽

Proximal Colon ◽

Sequencing Analysis ◽

Degrading Enzyme ◽

Vitro Model ◽

Long Chain

Long-chain arabinoxylans (LC-AX) are degraded in the colon by intestinal bacteria possessing AX-degrading enzymes, such as bifidobacteria. Enzymatic activity of intestinal bacterial might vary depending on the composition of the gut microbiota. To compare the enzymatic activities of the bacterial gut communities of two healthy individuals (donors D1 and D2), these bacterial communities were inoculated into in vitro model M-SHIME®. Differences in xylanase activities and denaturing gradient gel electrophoresis profiles, in particular a DNA-band corresponding with Bifidobacterium longum, were found in the proximal colon vessel. 16S rRNA gene sequencing analysis demonstrated the presence of two different B. longum species in these bacterial communities, showing 99% gene sequence similarity with B. longum NCC2705 and B. longum. subsp. longum KACC 91563, respectively, further referred to as B. longum D1 and B. longum D2. When grown on LC-AX as the sole added energy source, B. longum D2 displayed significantly higher activities of β-xylanase (5.3-fold), β-xylosidase (2.9-fold), and α-arabinofuranosidase (1.5-fold), respectively, compared to B. longum D1. When B. longum D2 was inoculated in the M-SHIME, inoculated with the bacterial gut communities of the individual with low AX-degrading enzyme activities, the β-xylanase activity increased (1.5-fold) in the proximal vessel. We demonstrated the presence of differences in LC-AX degrading enzyme activities of the bacterial gut communities of two individuals in the in vitro M-SHIME model, which could be linked to the presence of a potent AX-degrading B. longum (D2) strain.

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An in vitro model for investigation of vitamin A effects on wound healing

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000692 ◽

2021 ◽

pp. 1-6

Author(s):

Hoda Keshmiri Neghab ◽

Mohammad Hasan Soheilifar ◽

Gholamreza Esmaeeli Djavid

Keyword(s):

Wound Healing ◽

Endothelial Cell ◽

Vitamin A ◽

In Vitro Model ◽

Cellular Proliferation ◽

Endothelial Cell Migration ◽

Normal Fibroblast ◽

Anti Inflammatory ◽

Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inﬂammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inﬂammation responses.

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