scholarly journals Recovery of Lactobacillus casei strain Shirota (LcS) from faeces of healthy Singapore adults after intake of fermented milk

2019 ◽  
Vol 10 (7) ◽  
pp. 721-728
Author(s):  
W.W.T. Khine ◽  
X.J. Ang ◽  
Y.S. Chan ◽  
W.Q. Lee ◽  
S.Y. Quek ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Lactobacillus Casei ◽  
Culture Method ◽  
Fermented Milk ◽  
Stool Consistency ◽  
Dietary Habit ◽  
Study Condition ◽  
Rrna Sequencing ◽  
Before And After ◽  
Time Point

To validate survival of Lactobacillus casei strain Shirota (LcS) during passage through the gastrointestinal tract of healthy Singaporean young adults, 21 participants (18-25 years old) were asked to consume a 100 ml of fermented milk drink containing 1.0×108 cfu/ml of LcS daily for 14 days, and to maintain their dietary habit and life style. During and at the end of the ingestion period, both culture method (identity confirmed by ELISA) and 16s rRNA sequencing results revealed that viable LcS (7.27 and 7.64 log10 cfu/g of faeces at the ingestion period Day 7 and Day 14, respectively) and Lactobacillus could be recovered from the faeces of all the subjects. The viable LcS count from male and female were comparable for each time point. Before consumption (baseline) and 14 days after cessation of consumption of the fermented milk, LcS was not detected in most of the subjects. In this study condition, the composition of the major gut microbiota (>0.1% in relative abundance of genus) and characteristics of defaecation such as stool consistency and frequency of defecation did not change throughout the study before and after ingestion of LcS. LcS was able to survive passage through the gastrointestinal tract of Singapore adults without sustainable colonisation, but the effect of LcS on microbiota modulation, stool consistency and frequency was not observed under this study condition.

Effect of fermented milk containing Lactobacillus casei strain Shirota on constipation-related symptoms and haemorrhoids in women during puerperium

2015 ◽  
Vol 6 (3) ◽  
pp. 253-262 ◽  
Author(s):  
T. Sakai ◽  
H. Kubota ◽  
A. Gawad ◽  
L. Gheyle ◽  
S. Ramael ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Placebo Group ◽  
Lactobacillus Casei ◽  
Fermented Milk ◽  
Stool Consistency ◽  
Bowel Habit ◽  
Natural Childbirth ◽  
Probiotic Group ◽  
Abdominal Symptoms

Constipation and haemorrhoids are common complaints after childbirth. The objective of this pilot study was to evaluate impact of fermented milk containing Lactobacillus casei strain Shirota (LcS) on stool consistency and frequency, constipation-related symptoms and quality of life, and incidence of haemorrhoids in women during puerperium. Forty women who had natural childbirth were randomised to group consuming either one bottle/day of fermented milk containing at least 6.5×109 cfu of LcS, or placebo, for 6 weeks after childbirth. Subjects filled in a diary on their bowel habits including number of bowel movement, stool consistency and incidence of haemorrhoids, and answered questionnaires on constipation-related symptoms (PAC-SYM) and quality of life (PAC-QOL) during the study period. The probiotic group showed the better scores on overall PAC-SYM (P=0.013), PAC-SYM subscales of abdominal symptoms (P=0.043) and rectal symptoms (P=0.031), and PAC-QOL satisfaction subscale (P=0.037) in comparison with the placebo group. In the probiotic group, two to four subjects experienced haemorrhoids during the first 3 weeks of treatment. The number decreased in week 4 and no one had haemorrhoids on most days in week 5-6. In the placebo group, on average four subjects had haemorrhoids from the beginning, and no obvious change was observed until week 6. No statistically significant effect was observed on stool consistency and frequency. The study products did not cause any adverse event in the subjects. Results of this study indicate that continuous consumption of fermented milk containing LcS might alleviate constipation-related symptoms, provide satisfactory bowel habit and result in earlier recovery from haemorrhoids in women during puerperium. Nonetheless, there are several limitations in interpretation of the results attributed to the study design, including lack of baseline data. Further study is required in order to confirm the efficacy.

scholarly journals Daily Intake of Fermented Milk Containing Lactobacillus casei Shirota (Lcs) Modulates Systemic and Upper Airways Immune/Inflammatory Responses in Marathon Runners

Nutrients ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1678 ◽  
Author(s):  
Mauro Vaisberg ◽  
Vitoria Paixão ◽  
Ewin Almeida ◽  
Juliana Santos ◽  
Roberta Foster ◽  
...  
Keyword(s):  
Placebo Group ◽  
Antimicrobial Peptides ◽  
Nasal Mucosa ◽  
Lactobacillus Casei ◽  
Inflammatory Responses ◽  
Fermented Milk ◽  
Marathon Runners ◽  
Time Points ◽  
Lactobacillus Casei Shirota ◽  
Before And After

Background. Although Lactobacillus casei Shirota (LcS) can benefit the immune status, the effects of LcS in the immune/inflammatory responses of marathon runners has never been evaluated. Therefore, here we evaluated the effect of daily ingestion of fermented milk containing or not LcS in the systemic and upper airway immune/inflammatory responses before and after a marathon. Methods. Forty-two male marathon runners ingested a fermented milk containing 40 billion of LcS/day (LcS group, n = 20) or placebo (unfermented milk, n = 22) during 30 days pre-marathon. Immune/inflammatory parameters in nasal mucosa and serum, as well as concentrations of secretory IgA (SIgA) and antimicrobial peptides in saliva, were evaluated before and after fermented milk ingestion, immediately, 72 h, and 14 d post-marathon. Results. Higher proinflammatory cytokine levels in serum and nasal mucosa, and also lower salivary levels of SIgA and antimicrobial peptides, were found immediately post-marathon in the placebo group compared to other time points and to LcS group. In opposite, higher anti-inflammatory levels and reduced neutrophil infiltration on nasal mucosa were found in the LcS group compared to other time points and to the placebo group. Conclusion. For the first time, it is shown that LcS is able to modulate the systemic and airways immune responses post-marathon.

Obesity management through Ayurveda - A Case Study

2017 ◽  
Vol 2 (4) ◽  
Author(s):  
Dheeraj Kumar Tyagi ◽  
Shivakumar .
Keyword(s):  
Clinical Findings ◽  
Dietary Habit ◽  
Unhealthy Lifestyle ◽  
Lifestyle Management ◽  
Before And After ◽  
Effect Of Treatment ◽  
And Control

Lifestyle disorders are one of the biggest threats for the population living unhealthy lifestyle. Sthoulya (Obesity) is one such disorder which creates lot of physical as well as mental disorder to the sufferer. Due to changing lifestyle, comforts and dietary habit lots of individuals changed their life totally. Obesity is a growing disease in developed and developing countries. Prevalence is drastically hike in past few years. Ayurveda, the science of life with which we can manage and control lots of lifestyle disorders. Focusing on dietary and lifestyle management along with treatment, we can overcome the hazards of obesity which is growing in a uncontrolled manner. The available data is based on the clinical findings only. Aim and objective: To assess the effect of “Guru Cha Atarpanam Chikitsa” in the management of Sthoulya. Setting: Swastharakshana evam Yoga, OPD and IPD, SDMCAH, Hassan. Method: Udwarthana, Parisheka, Shamana Aushadhis, Ahara, and Vihara was followed within the treatment duration and effect of treatment was assessed before and after treatment, advised for follow up. Results: The treatment adopted is effective in the management of Sthoulya and to improve the quality of life.

scholarly journals How to measure fluctuating impairments in people with MS: development of an ambulatory assessment version of the EQ-5D-5L in an exploratory study

2021 ◽  
Author(s):  
Christine Blome ◽  
Jill Carlton ◽  
Christoph Heesen ◽  
Mathieu F. Janssen ◽  
Andrew Lloyd ◽  
...  
Keyword(s):  
Exploratory Study ◽  
Iterative Process ◽  
Face Validity ◽  
Ambulatory Assessment ◽  
Standard Assessment ◽  
Before And After ◽  
Standard Paper ◽  
Subsequent Wave ◽  
Patients Perspective ◽  
Time Point

Abstract Background Health fluctuations even within a single day are typical in multiple sclerosis (MS), but are not captured by widely used questionnaires like the EQ-5D-5L. This exploratory study aimed to develop an ambulatory assessment (AA) version of the EQ-5D-5L (EQ-5D-AA) where patients rate their health on mobile phones multiple times per day over several days, and to assess its feasibility and face validity. Methods An initial EQ-5D-AA version was based on two patient focus groups. It was then tested and continuously developed in an iterative process: patients completed it over several days, followed by debriefing interviews. Findings were used to refine the EQ-5D-AA, with the resulting version being tested by the subsequent wave of patients until participants declared no need for changes anymore. Before and after the AA period, participants completed the standard paper-based EQ-5D-5L asking about ‘today’. Results Focus group participants reported that their impairments often fluctuated between and within days. They regarded an AA with three assessments per day over seven days most appropriate; assessment should be retrospective to the previous assessment, but not all items should be assessed at each time point. Four waves of AA testing were conducted. Thirteen out of the 17 participants preferred the AA over standard assessment as they regarded it more informative, but not too burdensome. Conclusion The newly developed one-week AA of the EQ-5D-5L captures within-day and day-to-day health fluctuations in people with MS. From the patients’ perspective, it is a feasible and face valid way to provide important information beyond what is captured by the standard EQ-5D-5L.

Rehydration with a Caffeinated Beverage during the Nonexercise Periods of 3 Consecutive Days of 2-a-Day Practices

2004 ◽  
Vol 14 (4) ◽  
pp. 419-429 ◽  
Author(s):  
Kelly A. Fiala ◽  
Douglas J. Casa ◽  
Melissa W. Roti
Keyword(s):  
Body Fat ◽  
Hydration Status ◽  
Double Blind ◽  
Psychological Measures ◽  
Mild Heat ◽  
Urine Color ◽  
Significant Difference ◽  
Before And After ◽  
Time Point

The purpose of this study was to assess the influence of rehydration with a caffeinated beverage during non exercise periods on hydration status throughout consecutive practices in the heat. Ten (7 women, 3 men) partially heat-acclimated athletes (age 24 ± ly, body fat 19.2 ± 2%, weight 68.4 ± 4.0 kg, height 170 ± 3 cm) completed 3 successive days of 2-a-day practices (2 h/ practice, 4 h/d) in mild heat (WBGT = 23 °C). The 2 trials (double-blind, random, cross-over design) included; 1) caffeine (CAF) rehydrated with Coca-Cola® and 2) caffeine-free (CF) rehydrated with Caffeine-Free Coca-Cola®. Urine and psychological measures were determined before and after each 2-h practice. A significant difference was found for urine color for the post-AM time point, F = 5.526, P = 0.031. No differences were found among other variables (P > 0.05). In summary, there is little evidence to suggest that the use of beverages containing caffeine during non exercise might hinder hydration status.

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