Effect of Mangosteen Peel, Garlic and Urea Pellet Supplementation on Rumen Fermentation and Microbial Protein Synthesis of Beef Cattle

Abstract Background: Good quality protein as an on-farm feed resources has been in great demand to support the productivity of ruminants. A digestion trial using beef cattle crossbreds was conducted to assess the four dietary treatments of Flemingia macrophylla silage (FMS) supplementation at 0, 0.2, 0.4 and 0.6 kg dry matter (DM)/day in a 4 × 4 Latin square design. Feed DM intakes were measured during the 14 days and sample of feeds, feces, urine, as well as rumen fluid, blood were collected during the 7 days while the animals were on metabolism crates.Results: Based on this experiment revealed that strategic supplementation of FMS increased (P < 0.05) nutrients digestibility (organic matter, crude protein, and acid detergent fiber) enhanced rumen total volatile fatty acid production especially propionic acid (C3), C2:C3 ratio while, remarkably promoted the microbial protein synthesis (MPS) by increasing N-balance and retention of purine derivatives.Conclusion: Under this experiment, the results revealed the potential use of FMS as a good-quality feed to improve nutrients digestibility, rumen fermentation, microbial protein synthesis, and to mitigate methane production. FMS supplementation at 0.6 kg DM/day exhibited the best result.

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Strategic supplementation of Flemingia silage to enhance rumen fermentation efficiency, microbial protein synthesis and methane mitigation in beef cattle

BMC Veterinary Research ◽

10.1186/s12917-020-02703-x ◽

2020 ◽

Vol 16 (1) ◽

Author(s):

Bounnaxay Viennasay ◽

Metha Wanapat

Keyword(s):

Protein Synthesis ◽

Beef Cattle ◽

Rumen Fluid ◽

Latin Square ◽

Rumen Fermentation ◽

Microbial Protein ◽

Fatty Acid Production ◽

Microbial Protein Synthesis ◽

Dietary Treatments ◽

Nutrients Digestibility

Abstract Background Good quality protein as an on-farm feed resource has been in great demand to support the productivity of ruminants. A digestion trial using beef cattle crossbreds was conducted to assess the four dietary treatments of Flemingia macrophylla silage (FMS) supplementation at 0, 0.2, 0.4 and 0.6 kg dry matter (DM)/day in a 4 × 4 Latin square design. Feed DM intakes were measured during the 14 days and sample of feeds, feces, urine, as well as rumen fluid and blood were collected during the 7 days while the animals were on metabolism crates. Results Based on this experiment strategic supplementation of FMS increased (P < 0.05) nutrients digestibility (organic matter, crude protein, and acid detergent fiber) enhanced rumen total volatile fatty acid production especially propionic acid (C3), C2:C3 ratio while, remarkably promoted the microbial protein synthesis (MPS) by increasing N-balance and retention of purine derivatives. Conclusions Under this experiment, the results revealed the potential use of FMS as a good-quality feed to improve nutrients digestibility, rumen fermentation, microbial protein synthesis, and to mitigate methane production. FMS supplementation at 0.6 kg DM/day exhibited the best result.

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Effect of pine-based biochars with differing physiochemical properties on methane production, ruminal fermentation, and rumen microbiota in an artificial rumen (RUSITEC) fed barley silage

Canadian Journal of Animal Science ◽

10.1139/cjas-2020-0129 ◽

2021 ◽

pp. 1-13

Author(s):

Paul Tamayao ◽

Gabriel O. Ribeiro ◽

Tim A. McAllister ◽

Kim H. Ominski ◽

Atef M. Saleem ◽

...

Keyword(s):

Protein Synthesis ◽

Fixed Effects ◽

Block Design ◽

Rumen Fermentation ◽

Ruminal Fermentation ◽

Microbial Protein ◽

Rumen Microbiota ◽

Alpha And Beta Diversity ◽

Microbial Protein Synthesis ◽

Barley Silage

This study investigated the effects of three pine-based biochar products on nutrient disappearance, total gas and methane (CH4) production, rumen fermentation, microbial protein synthesis, and rumen microbiota in a rumen simulation technique (RUSITEC) fed a barley-silage-based total mixed ration (TMR). Treatments consisted of 10 g TMR supplemented with no biochar (control) and three different biochars (CP016, CP024, and CP028) included at 20 g·kg−1 DM. Treatments were assigned to 16 fermenters (n = 4 per treatment) in two RUSITEC units in a randomized block design for a 17 d experimental period. Data were analyzed using MIXED procedure in SAS, with treatment and day of sampling as fixed effects and RUSITEC unit and fermenters as random effects. Biochar did not affect nutrient disappearance (P > 0.05), nor total gas or CH4, irrespective of unit of expression. The volatile fatty acid, NH3-N, total protozoa, and microbial protein synthesis were not affected by biochar inclusion (P > 0.05). Alpha and beta diversity and rumen microbiota families were not affected by biochar inclusion (P > 0.05). In conclusion, biochar did not reduce CH4 emissions nor affect nutrient disappearance, rumen fermentation, microbial protein synthesis, or rumen microbiota in the RUSITEC.

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