Effect of mangosteen peel extract on BPA-exposed murine during gestation

Aim: To evaluate the potential protective effects of mangosteen peel extract against BPA-induced abnormalities on-pregnant mice fetus at implantation stage and offspring at post-parturition. Methodology: Pregnant mice were orally administered with BPA (100mg kg-1 b.wt.) and mangosteen peel extract (200mg kg-1 b.wt.) for 16 days. In order to evaluate the effect of MPE treatment on fetus at implantation stage, the pregnant mice were euthanized at day 18 and the fetus number and morphology were examined. Another group of treated dams, were allowed to undergo parturition for evaluating the of maternal weight, litter size and offspring sex-skewness. Results: Upon feeding Mangosteen peel extract (MPE), the average daily weight gain of dams were not significantly different from the control and BPA treated dams. The fetus derived from BPA treated dams were detected with abnormalities such as under development, haemorrhage and absence of vein, whereas fetus from dam treated with MPE and BPA as well as control were normal. The average litter size of all the treatment groups were not significantly different from the control group. BPA treated mice had lower pups survival up to 6 weeks compared to the groups treated with MPE and control. Test of proportion analysis showed BPA-treated group had significantly higher fraction female ratio. Interpretation: BPA is known as endocrine disruptor causing oxidative stress to female reproductive system, hence mangosteen peel extract contains antioxidant substances that have the potential to ameliorate the adverse effects of BPA exposure on dams during pregnancy and its fetus development.

Test of the Effectiveness of Anti-Dyslipedemia Extract Ethanol Mangosteen Peel (Garcinia Mangostana L.) in Male Mice Fed a High-Fat Diet

Dyslipidemia is a major risk factor for atheroscler heart disease, stroke, and is often defined as abnormalities or disruption of lipid metabolism. Garcinia mangostana L. is famous for its anti-inflammatory properties and is used in the treatment of skin infections and wounds. The main phytochemicals present in this species are anisoprenylated xanthone, many reports of biological effects, such as antioxidant, pro-apoptosis, anti-proliferative, anti-nosiseptif, anti-inflammatory, neuroprotective, hypoglycemic, and anti-obesity. This study aims to find out the effectiveness of mangosteen peel ethanol extract as an anti-dyslipidemia in male wistar rats given a high-fat diet. This type of research is experimental with a Pre-test approach and Post-test group only control design. The samples used were mangosteen peel ethanol extract and male wistar rats, with the size of the sample calculated with Federer's formula. Analyze data with the One-Way Anova Test if the data is normally distributed with advanced tests in the form of Post Hoc Tukey HSD tests to see real differences between treatments. The results of the total cholesterol study in the entire treatment group of mice showed a significant difference in P values < 0.05. Triglyceride levels in the entire treatment group also showed significant differences, this can be seen from the value of P < 0.05 (Value P = 0.029). LDL levels also showed significant differences across the treatment group, which can be seen from the P value of < 0.05. HDL levels make a significant difference in the value of P < 0.05 (Value P = 0.029). SGOT and SGPT levels in the entire group of rat treatment showed significant differences, this was seen from the value of P < 0.05. The conclusion that mangosteen peel ethanol extract significantly lowered total cholesterol, triglyceride levels, LDL levels, SGOT levels compared to the control group. Mangosteen peel ethanol extract can significantly increase HDL levels compared to the control group.

Antibacterial, Antioxidation, UV-Blocking, and Biodegradable Soy Protein Isolate Food Packaging Film with Mangosteen Peel Extract and ZnO Nanoparticles

The objective of this study was to prepare a functional biodegradable soy protein isolate (SPI) food packaging film by introducing a natural antimicrobial agent, mangosteen peel extract (MPE, 10 wt% based on SPI), and different concentrations of functional modifiers, ZnO NPs, into the natural polymer SPI by solution casting method. The physical, antioxidant, antibacterial properties and chemical structures were also investigated. The composite film with 5% ZnO NPs had the maximum tensile strength of 8.84 MPa and the lowest water vapor transmission rate of 9.23 g mm/m2 h Pa. The composite film also exhibited excellent UV-blocking, antioxidant, and antibacterial properties against Escherichia coli and Staphylococcus aureus. The TGA results showed that the introduction of MPE and ZnO NPs improved the thermal stability of SPI films. The microstructure of the films was analyzed by SEM to determine the smooth surface of the composite films. ATR-FTIR and XPS analyses demonstrated the strong hydrogen bonding of SPI, MPE, and ZnO NPs in the films. The presence of ZnO NPs in the composite films was also proved by EDX and XRD. These results suggest that SPI/MPE/ZnO composite film is promising for food-active packaging to extend the shelf life of food products.

THE ENHANCED PHOTOCATALYTIC PROPERTIES OF SILVER PHOSPHATE SYNTHESIZED UNDER MANGOSTEEN PEEL EXTRACT SOLUTION

The synthesis of silver phosphate (Ag3PO4) photocatalyst has been widely developed for organic pollutant degradation. However, the large particle of this photocatalyst limits the photocatalytic activity. The smaller particle size of the Ag3PO4 photocatalyst was successfully prepared using the starting material of AgNO3 and Na2HPO4.12H2O under mangosteen peel extract solution. The starting materials were dissolved in mangosteen peel extract solutions prepared at the concentration of 1% (w/v). The reaction of silver nitrate and phosphate solution was conducted at room temperature. The samples of pristine Ag3PO4 and Ag3PO4 prepared under mangosteen peel extract were studied using XRD, DRS, SEM, BET, and FTIR. All photocatalytic activities were evaluated using Rhodamine B photooxidation under blue light irradiation (LED, 3 Watt). The results showed that the mangosteen peel extract significantly decreased the particle size, lowered the bandgap energy from 2.12 to 2.00 eV, and increased the crystallinity of Ag3PO4. The interaction of xanthones from mangosteen peel extract solution with silver ion might affect the growth particle of Ag3PO4, and inhibit the agglomeration leading to small particle size, more uniform distribution, high crystallinity, and low bandgap energy. These properties enhanced the photocatalytic activity up to 2.9 times higher compared to the sample without the treatment of mangosteen peel extract.

Inhibition test of mangosteen peel extract (Garcinia mangostana L.) against bacterial diseases of tiger grouper (Epinephellus fuscoguttatus)

Cultivation of tiger grouper (Epinephelus fuscoguttatus) in Floating Net Cages in several locations in Bireuen Regency has developed. The potential disease issue can be caused by bacteria, viruses, fungi, or parasites. The purpose of this study was to determine the effectiveness of mangosteen peel extract capsules (Garcinia mangostana L) against bacterial disease in tiger grouper. This research was carried out in April 2019 at the MIPA Laboratory of Al Muslim University and the laboratory of PT. Centra Proteina Prima, Bireuen Regency. The design used was a completely randomized design with four treatments (0%, 10%, 20%, and 30% mangosteen peel extract capsules) with three replications. The parameters studied in this study including the diameter of the inhibition and the observation of MBC (Minimum Bacterial Concentration). The result showed that the administration of mangosteen peel extract capsules with doses of 10%, 20%, and 30% has no significant effect on reducing disease-causing bacteria in grouper grown on TSA media. Positive results were obtained on positive (+) MBC observations, presumably due to the small dose of mangosteen peel extract capsules given so that it could not kill bacteria grown in dishes with an inhibition range of 0 mm.Introduction

Acetobacter garciniae sp. nov., an acetic acid bacterium isolated from fermented mangosteen peel in Thailand

Two isolates, MS16-SU-2T and MS18-SU-3, obtained from fermented mangosteen peel in vinegar were suggested to constitute a new species assignable to the genus Acetobacter based on the results of 16S rRNA gene sequencing. The two isolates showed the highest sequence similarity (98.58%) to Acetobacter tropicalis NBRC 16470T and Acetobacter senegalensis LMG 23690T. However, the calculated similarity values were lower than the threshold for species demarcation. The phylogenetic analysis showed that the branches of the two isolates were separated from other Acetobacter species, and the two isolates constituted a new species in the genus Acetobacter . The genomic DNA of isolate MS16-SU-2T was sequenced. The assembled genome of the isolate was analysed, and the results showed that the highest average nucleotide identity value of 75.9 % was with Acetobacter papayae JCM 25143T and the highest digital DNA–DNA hybridization value of 25.1 % was with Acetobacter fallax LMG 1636T, which were lower than the cutoff values for species delineation. The phylogenetic tree based on the genome sequences showed that the lineage of isolate MS16-SU-2T was most closely related to A. papayae JCM 25143T and Acetobacter suratthaniensis TBRC 1719T, but separated from the branches of these two species. In addition, the two isolates could be distinguished from the type strains of closely related species by their phenotypic characteristics and MALDI-TOF profiles. Therefore, the two isolates, MS16-SU-2T (=TBRC 12339T=LMG 32243T) and MS18-SU-3 (=TBRC 12305), can be assigned to an independent species within the genus Acetobacter , and the name of Acetobacter garciniae sp. nov. is proposed for the two isolates.

Growth Performance, Meat Quality, Meat Oxidation and Intestinal Bacterial Contents of Broilers Fed with Garcinia mangostana Peel Extract

Background: Extracts of mangosteen (Garcinia mangostana) peels possess various biological activities such as antioxidants and anti-bacteria. The aim of this study was to investigate the effects of mangosteen peel extract (MPE) on growth performance, meat quality, oxidation of meat and bacterial contents in the small intestine of broilers. Methods: The 192, 7-day-old, chicks were allocated to 4 treatments with 3 replicates per treatment, each replicate containing 16 chicks. Birds were fed diets supplemented with 0, 0.2, 0.4 or 0.8% of MPE for a period of 5 weeks ad libitum. Growth performance was evaluated weekly. At 42 days old, the birds were slaughtered and the breast muscles were sampled from 4 birds per replicate for determining meat quality and oxidation. To determine bacterial quantities, the residue of feed in the small intestine (ilium and ceca) was sampled from one bird per replicate. Result: The results revealed that MPE had no significant effect on growth performance and intestinal bacterial contents. However, it was found that 0.4 to 0.8% of MPE could improve the meat quality by decreasing drip loss and total water loss in muscles. Moreover, 0.8% MPE group tended to be lower in lipid oxidation. This suggests that 0.8% MPE might protect the broiler breast meat against oxidation.