Effects of Inoculum and Substrate Concentrations in Anaerobic Fermentation of Treated Rice Bran to Acetone, Butanol and Ethanol

Mariculture organic waste (MOW) is rich in organic matter, which is a potential energy resource for anaerobic digestion. In order to enhance the anaerobic fermentation, the MOW was hydrolyzed by multi-enzyme and thermophilic bacteria. It was advantageous for soluble chemical oxygen demand (SCOD) release at MOW concentrations of 6 and 10 g/L with multi-enzyme and thermophilic bacteria pretreatments. For multi-enzyme, the hydrolysis was not obvious at substrate concentrations of 1 and 3 g/L, and the protein and carbohydrate increased with hydrolysis time at substrate concentrations of 6 and 10 g/L. For thermophilic bacteria, the carbohydrate was first released at 2–4 h and then consumed, and the protein increased with hydrolysis time. The optimal enzyme hydrolysis for MOW was determined by measuring the changes of SCOD, protein, carbohydrate, ammonia and total phosphorus, and comparing with acid and alkaline pretreatments.

Download Full-text

Anti-atherogenic and Nephroprotective Effects of Tocotrienol Rich Fraction (TRF) from Palm Oil and Rice Bran Oil

Planta Medica ◽

10.1055/s-0030-1251865 ◽

2010 ◽

Vol 76 (05) ◽

Author(s):

MR Khan ◽

S Siddiqui ◽

K Parveen ◽

WA Siddiqui

Keyword(s):

Rice Bran ◽

Palm Oil ◽

Rice Bran Oil ◽

Tocotrienol Rich Fraction

Download Full-text

The role of rice bran extract on acetyl CoA carboxylase in liver of rats fed a high-fat diet

Planta Medica ◽

10.1055/s-0031-1282786 ◽

2011 ◽

Vol 77 (12) ◽

Author(s):

C Charkhonpunya ◽

S Sireeratawong ◽

S Komindr ◽

N Lerdvuthisopon

Keyword(s):

Rice Bran ◽

High Fat Diet ◽

Acetyl Coa Carboxylase ◽

High Fat ◽

Acetyl Coa ◽

Rice Bran Extract

Download Full-text

Developing a nutraceutical from Egyptian stabilized rice bran: a pharmacological approach

Planta Medica ◽

10.1055/s-0031-1282630 ◽

2011 ◽

Vol 77 (12) ◽

Author(s):

AM Helal ◽

MT Khayyal ◽

HM Abd El Aziz ◽

RM Abdel Salam

Keyword(s):

Rice Bran ◽

Pharmacological Approach

Download Full-text

The comparative effect of rice bran extract on rats fed a high-fat diet for 4 versus 16 weeks

Planta Medica ◽

10.1055/s-0034-1394849 ◽

2014 ◽

Vol 80 (16) ◽

Author(s):

N Munkong ◽

S Sireeratawong ◽

A Wongnoppavich ◽

N Lerdvuthisopon

Keyword(s):

Rice Bran ◽

High Fat Diet ◽

High Fat ◽

Comparative Effect ◽

Rice Bran Extract

Download Full-text

Chromatographic Isolation of the LDH-Isoenzymes of Human Blood Platelets and an Investigation of Their Enzyme Kinetics

Thrombosis and Haemostasis ◽

10.1055/s-0038-1651273 ◽

1968 ◽

Vol 20 (03/04) ◽

pp. 301-313 ◽

Cited By ~ 2

Author(s):

W Schneider ◽

K Schumacher ◽

B Thiede ◽

R Gross

Keyword(s):

Human Blood ◽

Blood Platelets ◽

Deae Cellulose ◽

Order Reaction ◽

Kinetic Properties ◽

Ldh Isoenzymes ◽

Kinetic Investigations ◽

Substrate Concentrations ◽

Chromatographic Isolation ◽

Human Blood Platelets

SummaryThe LDH-isoenzymes of human blood platelets show a distinct predominance of the isoenzymes 2 and 3 upon chromatography on DEAE-cellulose. Small amounts of LDH-1 are also present, while only traces of LDH-4 and -5 can be detected.Enzyme kinetic investigations of the principal isoenzymes LDH-1, -2 and -3 clearly show that the differences in inhibition constants with pyruvate as substrate which are demonstrable at 25° largely disappear at 37°. On the other hand, the differences among the isoenzymes in their affinity for pyruvate and lactate as substrate as well as in with respect to the optimal substrate concentrations of pyruvate are more marked at 37° than at 25°. Also, the type of inhibition found with lactate as substrate is increasingly the expression of a higher order reaction in going from LDH-1 to LDH-3. A dependence of the LDH distribution pattern upon the metabolism of the cell is discussed. A comparison of our results with thrombocytes with those of other workers with erythrocytes and leucocytes makes it unlikely that the LDH pattern is directly dependent upon the existence of an oxidative metabolism. Rather, the redox potential of the cell could be of importance for the nature of the pattern of isoenzymes and for their differing kinetic properties.

Download Full-text

THE INFLUENCE OF ETHANOL ON THE CONVERSION OF PREGNENOLONE TO PROGESTERONE BY HUMAN PLACENTAL MICROSOMES

Acta Endocrinologica ◽

10.1530/acta.0.0760178 ◽

1974 ◽

Vol 76 (1) ◽

pp. 178-188 ◽

Cited By ~ 3

Author(s):

H. Lübbert ◽

K. Pollow ◽

R. Wagner ◽

J. Hammerstein

Keyword(s):

Kinetic Parameters ◽

Enzyme Preparation ◽

Ethanol Concentration ◽

Hydroxysteroid Dehydrogenase ◽

Product Formation ◽

Linear Increase ◽

Maximal Velocity ◽

Microsomal Enzyme ◽

Temperature Optima ◽

Substrate Concentrations

ABSTRACT The effects of ethanol on kinetic parameters of placental Δ5-3β-hydroxysteroid dehydrogenase were studied. In the presence of high pregnenolone concentrations (50 μm, [S] > Km) the microsomal enzyme preparation exhibited an almost linear increase in activity as the ethanol concentration in the medium was raised from 2.5 to 15 % (v/v). At lower substrate concentrations ([S] << Km) ethanol caused inhibition. Other effects of ethanol were: linearity of product formation with time was prolonged; the maximal velocity was markedly increased; the Km for pregnenolone slightly decreased with increasing ethanol concentrations (2.5 to 10 %, v/v) whereas the Km for NAD remained the same. The pH and temperature optima of the reaction were unaffected by ethanol. Other organic solvents caused similar effects.

Download Full-text