In vitro Cytotoxic Activity of Dendrophthoe falcata on Human Breast Adenocarcinoma Cells-MCF-7

2010 ◽  
Vol 7 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Nipun Dashora ◽  
Vijay Sodde ◽  
Kirti S. Prabhu ◽  
Richard Lobo
Keyword(s):  
Cytotoxic Activity ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
Adenocarcinoma Cells ◽  
Dendrophthoe Falcata ◽  
Mcf 7

scholarly journals Preliminary phytochemical screening and anticancer potential of Kohautia aspera (Heyne Ex Roth) Bremek

2019 ◽  
Vol 10 (3) ◽  
pp. 2502-2506
Author(s):  
Kavitha G ◽  
Sivakkumar T ◽  
Elessy Abraham
Keyword(s):  
Breast Adenocarcinoma ◽  
Phytochemical Screening ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
Percolation Method ◽  
Adenocarcinoma Cells ◽  
Successive Extraction ◽  
Potential Evaluation ◽  
Mcf 7

Phytochemical screening followed by anticancer potential evaluation of Kohautia aspera (Heyne ex Roth) Bremek was the purpose of the study. Successive extraction was performed with four solvents of different polarity as well as hot percolation method for the aqueous extract. The anticancer potential of the plant was investigated by standard MTT assay against (MCF-7) human breast adenocarcinoma cells. The extracts showed many phytoconstituents. In vitro cytotoxic activity of plant increased with increasing concentration of extracts. The presence of important phytoconstituents in plants may provide protection against a number of diseases. The investigation concludes that Kohautia aspera (Heyne ex Roth) Bremek possesses significant anticancer potential.

scholarly journals Change in the Expression of BAK، FAS، BAX، TNF-A، BCL-2 and Survivin Apoptosis Genes of the Human Breast Adenocarcinoma Cells (MCF-7) by Staphylococcal Enterotoxin B

2019 ◽  
Author(s):  
Sara Afzali ◽  
Abbas Doosti ◽  
Mansour Heidari ◽  
Nahid Babaei ◽  
Parvaneh Keshavarz
Keyword(s):  
Staphylococcal Enterotoxin ◽  
Breast Adenocarcinoma ◽  
Control Group ◽  
Staphylococcal Enterotoxin B ◽  
Type B ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
Adenocarcinoma Cells ◽  
Enterotoxin B ◽  
Mcf 7

Introduction: Breast cancer is one of the most prevalent malignancies among women. Patients whose suffering from this condition, as a result of the use of conventional therapies often have a poor response to treatment and the relapse among them is frequent. In this study, the effects of staphylococcal enterotoxin type B on BAK، FAS، BAX، TNF-a، BCL-2 و Survivin genes expression in human breast adenocarcinoma cells (MCF-7) was examined. Staphylococcal enterotoxin B is a powerful member of the Staphylococcus aureus toxins family, which is known as an anticancer agent with potential for killing cancer cells. Methods: The experimental study was carried out at the Biotechnology Research Center of Islamic Azad University, Shahrekord Branch. By using Lipofectamine 2000 reagent, MCF-7  cells transfected with the pcDNA3.1(+)-seb (recombinant) and  pcDNA3.1(+) (non-recombinant)  plasmids and were selected by culturing in a selective medium of RPMI- 1640 containing 600 μg / mL antibiotic G418. Then, the expression of BAK, FAS, BAX, TNF-a, BCL-2, and Survivin genes in transfected cells were analyzed by real time PCR. Student's t-test, SPSS Inc., Chicago, IL; Version 16 and also Excel program for statistical analysis were used. Results: The results of this study indicated that staphylococcal enterotoxin type B (SEB) remarkably changes the expression of apoptotic related genes in MCF-7 cell line. It was observed a significant increase in the expression of BAK, FAS, BAX, and TNF-a genes, the expression of BCL-2 and Survivin genes significantly decreased compared to the control group (P=0/032). Conclusion: Staphylococcal enterotoxin type B has an inhibitory effect on the growth, proliferation and invasion of breast adenocarcinoma cells through altering the expression of the genes involved in the apoptosis process. Therefore, it seems that there is a good research field for the use of this toxin in the control and treatment of human breast adenocarcinoma.

scholarly journals 3-{[(2,3-Dichlorophenyl)amino]methyl}-5-(furan-2-ylmethylidene)-1,3-thiazolidine-2,4-dione

Molbank ◽  
10.3390/m1083 ◽  
2019 ◽  
Vol 2019 (4) ◽  
pp. M1083
Author(s):  
Uwabagira ◽  
Sarojini
Keyword(s):  
Breast Adenocarcinoma ◽  
Cyclin Dependent Kinase ◽  
Human Breast ◽  
Hemolysis Assay ◽  
Human Breast Adenocarcinoma ◽  
Inflammatory Agent ◽  
Human Blood Cell ◽  
Anti Inflammatory ◽  
Mcf 7

The compound 3-{[(2,3-Dichlorophenyl)amino]methyl}-5-(furan-2-ylmethylidene)-1,3-thiazolidine-2,4-dione has been designed, synthesized, and screened for its in vitro antibreast cancer activity, using human breast adenocarcinoma cell lines (MCF-7) and in vitro anti-inflammatory activity. By hemolysis assay, it showed that it has a nonhemolytic and nontoxic effect on human blood cell. The title compound 5, subjected to in vitro activities, showed that it is cytotoxic with an IC50 of 42.30 µM and a good anti-inflammatory agent. The docking results against cyclin dependent kinase 2 (CDK2) (PDB ID: 3QQK) gave insights on its inhibitory activity.

Photodynamic activity of 2,6-diiodo-3,5-dithienylvinyleneBODIPYs and their folate-functionalized chitosan-coated Pluronic® F-127 micelles on MCF-7 breast cancer cells

2020 ◽  
Vol 24 (05n07) ◽  
pp. 973-984
Author(s):  
Nthabeleng Molupe ◽  
Balaji Babu ◽  
David O. Oluwole ◽  
Earl Prinsloo ◽  
Lizhi Gai ◽  
...  
Keyword(s):  
Breast Cancer Cells ◽  
Drug Delivery Systems ◽  
Quantum Yields ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
Viable Cells ◽  
Photodynamic Activity ◽  
Mcf 7

A 2,6-diiodo-3,5-dithienylvinyleneBODIPY dye was prepared and encapsulated with folate-chitosan capped Pluronic[Formula: see text] F-127 to provide drug delivery systems for photodynamic therapy (PDT). Moderately enhanced singlet oxygen quantum yields were observed for the dye encapsulation complexes in water. The in vitro dark cytotoxicity and photodynamic activity were investigated on the human breast adenocarcinoma (MCF-7) cell line. Minimal dark cytotoxicity was observed for the BODIPY dyes in 5% DMSO and when encapsulated in folate-functionalized chitosan-coated Pluronic[Formula: see text] F-127 micelles, since the cell viability values are consistently greater than 80% over the 0-40 [Formula: see text] concentration range. Upon irradiation of the samples, significant cytocidal activity was observed for the encapsulation complex of a 2,6-diiodo-8-dimethylaminophenyl-3,5-dithienylvinyleneBODIPY dye with less than 50% viable cells observed at concentrations [Formula: see text].

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