scholarly journals Methane Production and Methanogen Population in Rumen Liquor of Swamp Buffalo as Influenced by Coconut Oil and Mangosteen Peel Powder Supplementation

2011 ◽  
Vol 10 (19) ◽  
pp. 2523-2527 ◽  
Author(s):  
Ruangyote Pilajun ◽  
Metha Wanapat .
Keyword(s):  
Methane Production ◽  
Coconut Oil ◽  
Mangosteen Peel ◽  
Methanogen Population ◽  
Swamp Buffalo

scholarly journals Effects of Methanogenic Inhibitors on Methane Production and Abundances of Methanogens and Cellulolytic Bacteria inIn VitroRuminal Cultures

2011 ◽  
Vol 77 (8) ◽  
pp. 2634-2639 ◽  
Author(s):  
Zhenming Zhou ◽  
Qingxiang Meng ◽  
Zhongtang Yu
Keyword(s):  
Methane Production ◽  
Volatile Fatty Acids ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Cellulolytic Bacteria ◽  
Bacterial Populations ◽  
Content Type ◽  
Specific Pcr ◽  
Methanogen Population ◽  
Propynoic Acid

ABSTRACTThe objective of this study was to systematically evaluate and compare the effects of select antimethanogen compounds on methane production, feed digestion and fermentation, and populations of ruminal bacteria and methanogens usingin vitrocultures. Seven compounds, including 2-bromoethanesulphonate (BES), propynoic acid (PA), nitroethane (NE), ethyltrans-2-butenoate (ETB), 2-nitroethanol (2NEOH), sodium nitrate (SN), and ethyl-2-butynote (EB), were tested at a final concentration of 12 mM. Ground alfalfa hay was included as the only substrate to simulate daily forage intake. Compared to no-inhibitor controls, PA, 2NEOH, and SN greatly reduced the production of methane (70 to 99%), volatile fatty acids (VFAs; 46 to 66%), acetate (30 to 60%), and propionate (79 to 82%), with 2NEOH reducing the most. EB reduced methane production by 23% without a significant effect on total VFAs, acetate, or propionate. BES significantly reduced the propionate concentration but not the production of methane, total VFAs, or acetate. ETB or NE had no significant effect on any of the above-mentioned measurements. Specific quantitative-PCR (qPCR) assays showed that none of the inhibitors significantly affected total bacterial populations but that they did reduce theFibrobacter succinogenespopulation. SN reduced theRuminococcus albuspopulation, while PA and 2NEOH increased the populations of bothR. albusandRuminococcus flavefaciens. Archaeon-specific PCR-denaturing gradient gel electrophoresis (DGGE) showed that all the inhibitors affected the methanogen population structure, while archaeon-specific qPCR revealed a significant decrease in methanogen population in all treatments. These results showed that EB, ETB, NE, and BES can effectively reduce the total population of methanogens but that they reduce methane production to a lesser extent. The results may guide futureinvivostudies to develop effective mitigation of methane emission from ruminants.

Lipid-induced depression of methane production and digestibility in the artificial rumen system (RUSITEC)

1997 ◽  
Vol 77 (2) ◽  
pp. 269-278 ◽  
Author(s):  
Y. Dong ◽  
H. D. Bae ◽  
T. A. McAllister ◽  
G. W. Mathison ◽  
K-J. Cheng
Keyword(s):  
Methane Production ◽  
Canola Oil ◽  
Dry Matter ◽  
Bacterial Colonization ◽  
Coconut Oil ◽  
Metabolizable Energy ◽  
Microbial Populations ◽  
Molar Proportion ◽  
Fermentation Parameters ◽  
Key Words Lipids

An artificial rumen (RUSITEC) equipped with eight fermenters was used to examine the effects of canola oil (CA), cod liver oil (CD) and coconut oil (CO) on methane production, dietary digestibility and rumen microbial populations. The experiment, repeated three times, involved two diets (100% grass hay or a 90%:10% wheat:hay mixture) either untreated (controls) or sprayed with CA, CD or CO (10%, wt/wt) in a 2 × 4 factorial arrangement. Dry matter disappearance and CH4 production were greater (P < 0.05) from untreated concentrate diet than from untreated hay. There were significant interactions (P < 0.05) between diet and lipid type for DM digestion and several of the fermentation parameters. Coconut oil decreased (P < 0.05) DM and neutral detergent fibre disappearance of hay but did not affect digestion of concentrate. All oils, especially CO, depressed (P < 0.05) CH4 production (mmol g−1 digestible DM) and methanogenic populations regardless of diet. Methanogens on CO-treated diets numbered less than 1% of those on CA- and CD-treated diets. Total viable, amylolytic, and cellulolytic bacterial numbers were also substantially reduced (P < 0.05) by CO, but not by CA or CD for both diets. Bacterial colonization and digestion of CO-treated hay was markedly less extensive than that observed on untreated, CA- or CD-treated hay. The molar proportion of propionate produced was increased (P < 0.05) by CA and CD, for the hay diet, but not for the concentrate diet. Coconut oil decreased (P < 0.05) the molar proportion of propionate produced on the hay diet and increased (P < 0.05) it on the concentrate diet. Of the three oils, CO was most inhibitory to CH4 production and depressed fibre digestion. The CA and CD also decreased CH4 production, but did not affect feed digestion in either diet. Consequently, the amount of metabolizable energy of feeds may be increased when diets are supplemented with these oils. Key words: Lipids, methane, digestibility, artificial rumen, bacteria, VFA

Manipulation of ruminal fermentation and methane production by dietary saponins and tannins from mangosteen peel and soapberry fruit

2009 ◽  
Vol 63 (5) ◽  
pp. 389-400 ◽  
Author(s):  
Onanong Poungchompu ◽  
Metha Wanapat ◽  
Chalong Wachirapakorn ◽  
Sadudee Wanapat ◽  
Anusorn Cherdthong
Keyword(s):  
Methane Production ◽  
Ruminal Fermentation ◽  
Mangosteen Peel

scholarly journals The Effect of Different Concentrations of Total Polyphenols from Paulownia Hybrid Leaves on Ruminal Fermentation, Methane Production and Microorganisms

Animals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2843
Author(s):  
Julia Puchalska ◽  
Małgorzata Szumacher-Strabel ◽  
Amlan Kumar Patra ◽  
Sylwester Ślusarczyk ◽  
Min Gao ◽  
...  
Keyword(s):  
Methane Production ◽  
Crude Protein ◽  
Dry Matter ◽  
Protein Concentration ◽  
Microbial Population ◽  
Ruminal Fermentation ◽  
Total Polyphenols ◽  
Methanogen Population ◽  
Hydrogen Availability

This experiment was conducted to study the effects of different concentrations of polyphenols of Paulownia Clon In Vitro 112® leaves or their particular parts on in vitro ruminal fermentation, methane production and microbial population. Paulownia leaves with high (PLH; 31.35 mg/g dry matter (DM)), medium (PLM; 26.94 mg/g DM), and low level of polyphenols (PLL; 11.90 mg/g DM) were used from three plantation areas. Lamina (PLLA; 33.63 mg/g DM) and twigs (PLT; 2.53 mg/g DM) of leaves were also collected from the PLM plantation. The chemical analyses of Paulownia leaves indicated that the content of the most basic nutrients (e.g., crude protein concentration of 185 g/kg of DM) were similar to dehydrated alfalfa. The in vitro results showed that the use of Paulownia leaves with the highest content of total polyphenols (PLH and PLLA) decreased methane production, methanogens numbers, and acetate to propionate ratio. In PLT, lowered methane production was followed by reduced substrate degradability and volatile fatty acid (VFA) concentration along with higher acetate to propionate ratio. Therefore, reduction of methane production in PLH and PLLA was attributed to the lowered methanogen population, whereas in PLT it was caused by decreased substrate degradability with the resultant of limited hydrogen availability to the methanogens.

Manipulation of rumen fermentation and ecology of swamp buffalo by coconut oil and garlic powder supplementation

2011 ◽  
Vol 135 (1) ◽  
pp. 84-92 ◽  
Author(s):  
P. Kongmun ◽  
M. Wanapat ◽  
P. Pakdee ◽  
C. Navanukraw ◽  
Z. Yu
Keyword(s):  
Coconut Oil ◽  
Rumen Fermentation ◽  
Garlic Powder ◽  
Swamp Buffalo

scholarly journals Pengaruh Penambahan Minyak Kelapa, Minyak Biji Bunga Matahari, dan Minyak Kelapa Sawit terhadap Penurunan Produksi Metan di dalam Rumen secara in Vitro (The Effect of Addition Coconut Oil, Sunflower Seed Oil, and Palm Olein on Reducing Ruminal Methane Pro

2012 ◽  
Vol 33 (2) ◽  
pp. 96
Author(s):  
Puput Diah Sitoresmi ◽  
Lies Mira Yusiati ◽  
Hari Hartadi
Keyword(s):  
Methane Production ◽  
Sunflower Seed ◽  
Protein Concentration ◽  
Palm Olein ◽  
Seed Oil ◽  
Coconut Oil ◽  
Microbial Protein ◽  
Sunflower Seed Oil ◽  
Oil Sunflower

<p>This experiment was conducted to determine the effect of vegetable oil, such as coconut oil, sunflower seed oil, and palm olein on methane production, number of protozoa, microbial protein concentration, ammonia (NH3) concentration and carboxymethyl cellulase (CMC-ase) activity in the vitro fermentation of king grass and rice bran by rumen microbial. The experiment consisted of two treatments (i.e.) the effect of coconut oil, sunflower seed oil, and palm olein and level of addition of vegetable oil i.e. 0%, 2.5%, 5.0%, and 7.5%. The fermentation was done using Hohenheim gas test (HGT) metode and incubated at 39°C for 72 hours with three replicates. At the end of the fermentation, methane concentration, number of protozoa, microbial protein concentration, NH3 concentration, CMC-<br />ase activity, and pH were observed. Data obtained were analyzed using analysis of variance and the design using factorial (3x4). The deferences of mean values were analyzed by Duncan’s new multiple range test (DMRT). The result showed that the number of protozoa decreased (P&lt;0.05) as much as 9.8%, 20.85%, and 23.95%, followed by methane supression (P&lt;0.01) much as 11.11%, 15.79%, and 18.51% with oil addition at level 2.5%, 5.0%, and 7.5% compared to control, but no effect on microbial protein concentration, ammonia (NH3) concentration and carboxymethyl cellulase (CMC-ase) activity. It can be concluded that coconut oil had the highest affect on methane production by inhibition of<br />protozoa growth and addition oil up to 5.0% reduced methane production as much as 15.80%.</p><p>(Key words : Coconut oil, Sunflower seed oil, Palm olein, Methane production, Protozoa count, In vitro fermentation)<br /><br /></p>

scholarly journals Mangosteen peel can reduce methane production and rumen biohydrogenation in vitro

2016 ◽  
Vol 46 (4) ◽  
pp. 419 ◽  
Author(s):  
P Shokryzadan ◽  
M.A. Rajion ◽  
Y.M. Goh ◽  
I Ishak ◽  
M.F. Ramlee ◽  
...  
Keyword(s):  
Methane Production ◽  
Mangosteen Peel
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