Lipid-induced depression of methane production and digestibility in the artificial rumen system (RUSITEC)

1997 ◽  
Vol 77 (2) ◽  
pp. 269-278 ◽  
Author(s):  
Y. Dong ◽  
H. D. Bae ◽  
T. A. McAllister ◽  
G. W. Mathison ◽  
K-J. Cheng
Keyword(s):  
Methane Production ◽  
Canola Oil ◽  
Dry Matter ◽  
Bacterial Colonization ◽  
Coconut Oil ◽  
Metabolizable Energy ◽  
Microbial Populations ◽  
Molar Proportion ◽  
Fermentation Parameters ◽  
Key Words Lipids

An artificial rumen (RUSITEC) equipped with eight fermenters was used to examine the effects of canola oil (CA), cod liver oil (CD) and coconut oil (CO) on methane production, dietary digestibility and rumen microbial populations. The experiment, repeated three times, involved two diets (100% grass hay or a 90%:10% wheat:hay mixture) either untreated (controls) or sprayed with CA, CD or CO (10%, wt/wt) in a 2 × 4 factorial arrangement. Dry matter disappearance and CH4 production were greater (P < 0.05) from untreated concentrate diet than from untreated hay. There were significant interactions (P < 0.05) between diet and lipid type for DM digestion and several of the fermentation parameters. Coconut oil decreased (P < 0.05) DM and neutral detergent fibre disappearance of hay but did not affect digestion of concentrate. All oils, especially CO, depressed (P < 0.05) CH4 production (mmol g−1 digestible DM) and methanogenic populations regardless of diet. Methanogens on CO-treated diets numbered less than 1% of those on CA- and CD-treated diets. Total viable, amylolytic, and cellulolytic bacterial numbers were also substantially reduced (P < 0.05) by CO, but not by CA or CD for both diets. Bacterial colonization and digestion of CO-treated hay was markedly less extensive than that observed on untreated, CA- or CD-treated hay. The molar proportion of propionate produced was increased (P < 0.05) by CA and CD, for the hay diet, but not for the concentrate diet. Coconut oil decreased (P < 0.05) the molar proportion of propionate produced on the hay diet and increased (P < 0.05) it on the concentrate diet. Of the three oils, CO was most inhibitory to CH4 production and depressed fibre digestion. The CA and CD also decreased CH4 production, but did not affect feed digestion in either diet. Consequently, the amount of metabolizable energy of feeds may be increased when diets are supplemented with these oils. Key words: Lipids, methane, digestibility, artificial rumen, bacteria, VFA

scholarly journals Effects of Replacing Extruded Maize by Dried Citrus Pulp in a Mixed Diet on Ruminal Fermentation, Methane Production, and Microbial Populations in Rusitec Fermenters

Animals ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1316
Author(s):  
Jairo García-Rodríguez ◽  
Cristina Saro ◽  
Iván Mateos ◽  
Jesús S. González ◽  
María Dolores Carro ◽  
...  
Keyword(s):  
Methane Production ◽  
Microbial Growth ◽  
Microbial Populations ◽  
Mixed Diet ◽  
Ruminal Fermentation ◽  
Citrus Industry ◽  
Ph Values ◽  
Citrus Pulp ◽  
Liquid Phases ◽  
Fermentation Parameters

Citrus pulp is a highly abundant by-product of the citrus industry. The aim of this study was to assess the effects of replacing extruded maize (EM; 20% of total diet) by dried citrus pulp (DCP; 20%) in a mixed diet on rumen fermentation and microbial populations in Rusitec fermenters. The two diets contained 50% alfalfa hay and 50% concentrate, and the same protein level. Four Rusitec fermenters were used in a cross-over design with two 13-d incubation runs. After 7-d of diet adaptation, diet disappearance, fermentation parameters, microbial growth, and microbial populations were assessed. Fermenters receiving the DCP showed greater pH values and fiber disappearance (p < 0.001) and lower methane production (p = 0.03) than those fed EM. Replacing EM by DCP caused an increase in the proportions of propionate and butyrate (p < 0.001) and a decrease in acetate (p = 0.04). Microbial growth, bacterial diversity, and the quantity of bacteria and protozoa DNA were not affected by the diet, but the relative abundances of fungi and archaea were greater (p < 0.03) in solid and liquid phases of DCP fermenters, respectively. Results indicate that DCP can substitute EM, promoting a more efficient ruminal fermentation.

scholarly journals Energy partitioning and methane emission by sheep fed sorghum silages at different maturation stages

2015 ◽  
Vol 67 (3) ◽  
pp. 790-800 ◽  
Author(s):  
F.S. Machado ◽  
N.M. Rodríguez ◽  
L.C. Gonçalves ◽  
J.A.S. Rodrigues ◽  
M.N. Ribas ◽  
...  
Keyword(s):  
Energy Intake ◽  
Methane Production ◽  
Dry Matter ◽  
Methane Emissions ◽  
Energy Partitioning ◽  
Metabolizable Energy ◽  
Energy Utilization ◽  
Maturation Stage ◽  
Maturation Stages ◽  
Gross Energy

Energy partitioning and methane production by sheep fed silages of three commercially available sorghum hybrids (BRS 610, BR 700 and BRS 655) harvested at three maturation stages (milk, soft dough and floury) were evaluated in open circuit respiration chambers. A complete randomized design was used in a 3 × 3 (hybrids × maturity stages) factorial arrangement, and the means were compared by the Student-Newman-Keuls (SNK) test (P<0.05). The intake of dry matter, digestible dry matter, gross energy, digestible energy and metabolizable energy were not affected by maturation stage, but were influenced by hybrid. The net energy intake was influenced by maturity and sorghum genetics. The fecal output represented the main source of energy loss, as percentage of gross energy intake (48% to 52%), followed by heat increment (10% to 19%), methane emissions (4% to 6%) and urine (1% to 2%). There were no differences (P>0.10) among the treatments for the apparent digestibility of gross energy and metabolizability (qm). An interaction (P<0.05) between sorghum hybrid and maturation stages was observed for the efficiency of metabolizable energy utilization for maintenance (km), which ranged between 0.53 and 0.78. No differences (P>0.10) among treatments occurred in the daily methane production. There is substantial genetic diversity within sorghum species, determining different nutritional values. Sorghum genetics and maturity at harvest should not be an opportunity to reduce the contribution of agriculture to methane emissions.

Effects of barley silage particle size and concentrate level on rumen kinetic parameters and fermentation patterns in steers

2003 ◽  
Vol 83 (3) ◽  
pp. 533-539 ◽  
Author(s):  
H. W. Soita ◽  
D. A. Christensen ◽  
J. J. McKinnon
Keyword(s):  
Particle Size ◽  
Dry Matter ◽  
Ammonia Concentration ◽  
Rumen Fermentation ◽  
Neutral Detergent Fiber ◽  
Molar Proportion ◽  
Rumen Ph ◽  
Fermentation Parameters ◽  
Barley Silage ◽  
Concentrate Level

Four Hereford steers (471 ± 31 kg) fitted with ruminal cannulae were used in a 4 × 4 Latin square design to evaluate the effects and possible interactions between silage particle size and concentrate level on digestion and ruminal fermentation parameters. Diets were designed with two forage to concentrate ratios [low forage, 20:80, high forage, 50:50 on a dry matter (DM) basis], combined with two theoretical chop lengths of barley silage; short (SBS) = 4.7 mm and long (LBS) =18.8 mm. Results showed that steers fed diets with SBS consumed more DM than those fed LBS. An interaction (P < 0.05) between particle size and concentrate level was noted for molar proportion of acetate. At the higher concentrate level, feeding SBS diets reduced the molar proportion of acetate but increased the molar proportion of propionate. An interaction (P < 0.05) between particle size and concentrate level was noted for ruminal pH. When compared to LBS, the daily mean rumen pH was lower (P < 0.05) for steers fed SBS with 80% concentrate but not for those fed the 50% concentrate diet. Ruminal ammonia concentration was higher (P < 0.05) for those steers fed the 50% concentrate diets than for those fed the 80% concentrate diets. With high concentrate levels, SBS greatly reduced (P < 0.05) ammonia concentration. Passage rates for particulate matter and the retention time were decreased (P < 0.05) when SBS was fed. Feeding SBS reduced (P < 0.05) total tract digestibility of dry matter, crude protein (CP) and neutral detergent fiber (NDF) (65.5 vs. 68.5%, 66.5 vs. 73.4% and 39.4 vs. 45.9%), respectively. These results suggest that barley silage with a theoretical cut length of 18.8 mm can promote higher rumen pH, ammonia nitrogen, and reduced particulate passage rates. These changes in rumen fermentation parameters should favor optimum rumen function and digestion when high concentrate diets are fed. Key words: Barley silage, particle size, steers, concentrate level, rumen fermentation

scholarly journals Manipulation of Rice Straw Silage Fermentation with Different Types of Lactic Acid Bacteria Inoculant Affects Rumen Microbial Fermentation Characteristics and Methane Production

2021 ◽  
Vol 8 (6) ◽  
pp. 100
Author(s):  
Ehsan Oskoueian ◽  
Mohammad Faseleh Jahromi ◽  
Saeid Jafari ◽  
Majid Shakeri ◽  
Hieu Huu Le ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Rice Straw ◽  
Methane Production ◽  
Dry Matter ◽  
In Vitro Study ◽  
Microbial Populations ◽  
Different Types ◽  
Silage Quality

Bacterial inoculants are known to improve the quality of silage. The objectives of the present study were to evaluate the effects of different types of lactic acid bacteria (LAB; L. plantarum, L. salivarius, L. reuteri, L. brevi, and S. bovis) inoculation (106 cfu/ DM) on rice straw silage quality and to determine these effects on ruminal fermentation characteristics, digestibility and microbial populations in an in vitro condition. Inoculated rice straw was ensiled for 15 and 30 days. For the in vitro study, rumen fluid was obtained from three rumen-fistulated bulls fed on mixed forage and concentrate at 60:40 ratio twice daily. Inoculation with LAB improved (p < 0.05) the rice straw silage quality as indicated by higher dry matter and crude protein contents, decreased pH and butyric acid, and increased propionic acid and LAB numbers, especially after 30 days of ensiling. Results from the in vitro study revealed that starting with the addition of LAB to rice straw silage improved in vitro fermentation characteristics such as increased total volatile fatty acids and dry matter digestibility (p < 0.05). LAB treatments also decreased methane production and methane/total gas ratio after 15 and 30 days of ensiling. From the rumen microbial population perspective, cellulolytic, and fungal zoospores were enhanced, while protozoa and methanogens were decreased by the LAB treatments. Based on these results, it could be concluded that inoculating rice straw silage with LAB (especially for L. plantarum and S. bovis) improved silage quality, rumen fermentation parameters and microbial populations in vitro.

PSXIII-4 Effects of “País” grape marc on in vitro methane production and ruminal fermentation parameters of a forage diet

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 468-468
Author(s):  
Sandra Suescun-Ospina ◽  
Nelson Vera ◽  
Rita Astudillo ◽  
Jorge Avila-Stagno
Keyword(s):  
Methane Production ◽  
Dry Matter ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Corn Silage ◽  
Partial Substitution ◽  
Grape Marc ◽  
Ch4 Production ◽  
Fermentation Parameters

Abstract Grape marc (GM) is a viticulture by-product used as cattle supplement in periods of shortage of conventional feed sources. It contains fats, high concentrations of polyphenols and has been reported to reduce enteric methane (CH4) emissions. In-vitro batch culture was used to study the effects of substitution of mixed hay (MH) for a traditional Chilean variety (Vitis vinifera “País”) of GM on in vitro dry matter disappearance (IVDMD), rumen fermentation parameters (short chain fatty acids, pH, partitioning factor), gas and CH4 production in a 60% forage diet (dry matter, DM). The study was a randomized complete design with 3 treatments and 3 replicates, incubated for 24 h at 39º C. Treatments were: T1 (Control): 20% MH, 40% corn silage, 40% concentrate; T2 = 10% MH, 10% GM, 40% corn silage, 40% concentrate; T3 = 20% GM, 40% corn silage, 40% concentrate. Means were compared with the Tukey test (P &lt; 0.05), and polynomial contrasts. Substitution of MH with GM significantly reduced ammonia nitrogen (NH3-N) by 50% (P &lt; 0.05), although it did not affect IVDMD, gas production or other rumen fermentation parameters (P &gt; 0.05). Total CH4 (mg) linearly decreased (P = 0.013) as concentrations of GM increased. Methane production (mg/g DM incubated) and yield (mg/g DM digested) decreased linearly (P = 0.002 and P = 0.003, respectively) as inclusion of GM increased. Inclusion of GM at 20% reduced CH4 production by 19% and CH4 yield by 16.4%. These results indicate that partial substitution of dietary fiber sources with traditional Chilean País GM in high fiber diets is a viable feeding alternative, and can decrease environmental impact (lower CH4 and ammonia emissions) of ruminant livestock, without negatively affecting rumen fermentation parameters.

scholarly journals Predicting Metabolizable Energy from Digestible Energy for Growing and Finishing Beef Cattle and Relationships to Prediction of Methane

2022 ◽  
Author(s):  
K E Hales ◽  
C A Coppin ◽  
Z K Smith ◽  
Z S McDaniel ◽  
L O Tedeschi ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Methane Production ◽  
Dry Matter ◽  
Metabolizable Energy ◽  
Finishing Cattle ◽  
Digestible Energy ◽  
A Value ◽  
Wide Range ◽  
Data Points ◽  
Finishing Beef Cattle

Abstract Reliable predictions of metabolizable energy (ME) from digestible energy (DE) are necessary to prescribe nutrient requirements of beef cattle accurately. A previously developed database that included 87 treatment means from 23 respiration calorimetry studies has been updated to evaluate the efficiency of converting DE to ME by adding 47 treatment means from 11 additional studies. Diets were fed to growing-finishing cattle under individual feeding conditions. A citation-adjusted linear regression equation was developed where dietary ME concentration (Mcal/kg of dry matter [DM]) was the dependent variable and dietary DE concentration (Mcal/kg) was the independent variable: ME = 1.0001 × DE – 0.3926; r 2 = 0.99, root mean square prediction error [RMSPE] = 0.04, P &lt; 0.01 for the intercept and slope). The slope did not differ from unity (95% CI = 0.936 to 1.065); therefore, the intercept (95% CI = -0.567 to -0.218) defines the value of ME predicted from DE. For practical use, we recommend ME = DE – 0.39. Based on the relationship between DE and ME, we calculated the citation-adjusted loss of methane, which yielded a value of 0.2433 Mcal/kg of DMI (SE = 0.0134). This value was also adjusted for the effects of dry matter intake (DMI) above maintenance, yielding a citation-adjusted relationship: CH4, Mcal/kg = 0.3344 – 0.05639 × multiple of maintenance; r 2 = 0.536, RMSPE = 0.0245, P &lt; 0.01 for the intercept and slope). Both the 0.2433 value and the result of the intake-adjusted equation can be multiplied by DMI to yield an estimate of methane production. These two approaches were evaluated using a second, independent database comprising 129 data points from 29 published studies. Four equations in the literature that used DMI or intake energy to predict methane production also were evaluated with the second database. The mean bias was substantially greater for the two new equations, but slope bias was substantially less than noted for the other DMI-based equations. Our results suggest that ME for growing and finishing cattle can be predicted from DE across a wide range of diets, cattle types, and intake levels by simply subtracting a constant from DE. Mean bias associated with our two new methane emission equations suggests that further research is needed to determine whether coefficients to predict methane from DMI could be developed for specific diet types, levels of DMI relative to body weight, or other variables that affect the emission of methane.

scholarly journals Effect of Addition of Urea to The Barley Hay For Production of Gases In Vitro

2019 ◽  
Vol 12 (2) ◽  
Keyword(s):  
Organic Matter ◽  
Incubation Period ◽  
Methane Production ◽  
Dry Matter ◽  
Nitrogen Concentration ◽  
Ammonia Nitrogen ◽  
Metabolizable Energy ◽  
Barley Straw ◽  
Dry Matter Digestibility

The object of this study were determined the effect of adding urea (0,2%) and molasses (4%) to barley and straw on in vitro gas and methane production, in vitro fermentation characteristic (pH and ammonia nitrogen concentration) and in vitro digestibility, dry matter, organic matter and metabolizable energy, the samples incubated in 39oc water path for 24,48,72 and 96 h., the results indicated that there were significant decrease (p<0.01) in total gas and methane production after 48,72 and 96 h incubation periods in barley straw with 2% urea compared with control (without urea), while 24 h. total gas and methane production increased significant(p<0.01) in straw with 2% urea compared with control. However, metabolizable energy, organic matter digestibility, short chain fatty acids and metabolizable energy for lactation which determine by total gas production after 24 h. incubation increased significantly (p<0.01) in straw with 2% urea compared with no adding urea. The result showed no effects of treatments on pH in all incubation periods except 48h. were pH increased significantly (p<0.01) in compared with control straw with 2% urea .Ammonia nitrogen concentration increased significantly (p<0.01) in straw with 2% urea compared with control after 48h. of incubation period while control treatment increased significantly (p<0.01) after 96h. incubation period compared with straw with 2% urea in ammonia nitrogen concentration, no significant differences found in ammonia nitrogen concentration after 24 and 72 h. incubation period between treatments, moreover, the in vitro dry matter digestibility, organic matter digestibility and metabilizable energy increased significantly (p<0.01) in barley straw with 2% urea compared with control. The result indicated that adding 2% urea to barley straw increased in vitro gas and methane production which lead to increased in vitro organic and dry matter digestibility and metabolizable energy.

scholarly journals Application of Different Types of Lactic Acid Bacteria Inoculant on Ensiled Rice Straw; Effects on Silage Quality, Rumen Fermentation, Methane Production and Microbial Population

2019 ◽  
Author(s):  
Ehsan Oskoueian ◽  
Saeid Jafari ◽  
Reza Noura ◽  
Mohammad Faseleh Jahromi ◽  
Goh Yong Meng ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Rice Straw ◽  
Methane Production ◽  
Dry Matter ◽  
Microbial Population ◽  
Microbial Populations ◽  
Different Types ◽  
Silage Quality

AbstractBacterial inoculants are known to improve quality of silage. The objectives of the present study were to evaluate the effects of different types of lactic acid bacteria (LAB;L. plantarum,L. salivarius, L. reuteri, L. brevisandS. bovis) inoculation (106g−1DM) on rice straw silage quality and to examine these effects on ruminal fermentation characteristics, digestibility and microbial populations in anin vitrocondition. Inoculated rice straw was ensiled for 15 and 30 days. Forin vitrostudy, rumen liquor was obtained from two rumen fistulated mature cows fed on mixed forage and concentrate at 60:40 ratio twice daily. Inoculation of LAB improved (P˂0.05) the rice straw silage quality such as increased dry matter and crude protein contents, decreased pH and butyric acid, and increased propionic acid and LAB contents especially after 30 days of ensiling. Results fromin vitrostudy revealed that addition of LAB to the rice straw silage improved fermentation characteristics such as increased total volatile fatty acids and dry matter digestibility (P˂0.05). LAB treatments also decreased methane production and methane/total gas ratio after 15 and 30 days of ensiling. From the rumen microbial population perspective, cellulolytic, and fungal zoospores were enhanced while protozoa and methanogens were decreased by the LAB treatments. Based on these results, it could be concluded that inoculating rice straw silage with LAB (especially forL. plantarumandS. bovis) improved silage quality, rumen fermentation parameters and microbial populationsin vitro. However,in vivostudies need to confirm those effects.

Effects of Sericea Lespedeza on Feed Intake, Digestion, and Ruminal Methane Emission in Hair Sheep

2021 ◽  
Vol 99 (Supplement_2) ◽  
pp. 41-41
Author(s):  
Luana L Ribeiro ◽  
Ryszard Puchala ◽  
Arthur L Goetsch
Keyword(s):  
Methane Emission ◽  
Feed Intake ◽  
Dry Matter ◽  
Coconut Oil ◽  
Mixed Effects ◽  
Repeated Measure ◽  
Lespedeza Cuneata ◽  
Hair Sheep ◽  
Initial Body Weight ◽  
Sericea Lespedeza

Abstract Eighteen Katahdin (initial body weight of 74 kg; SEM=1.8) and 18 St. Croix ewes (55 kg; SEM=1.3) were used in an experiment with four 6-wk periods to determine effects of dietary level of Sericea lespedeza (Lespedeza cuneata) containing 5.8% condensed tannins (dry matter; DM) and other supplemental ingredients on feed intake, digestion, and ruminal methane emission. Diets were consumed ad libitum and included a concentrate supplement at 0.45% BW (DM). Alfalfa was the basal forage for control (CON), ionophore (ION; lasalocid at 33 mg/kg DM), coconut oil (3%; CCO), and soybean oil (3%; SBO) diets, and forage in moderate- and high-lespedeza diets was a 1:1 mixture of alfalfa and lespedeza and all lespedeza, respectively (MSL and HSL, respectively). Data were analyzed with a 2 x 6 factorial arrangement of treatments, period as a repeated measure, and a mixed effects model. Digestion and methane emission were determined in weeks 4, 10, 16, and 22. Total DM intake was similar among treatments (P = 0.070) but numerically greatest for HSL (1,197, 1,297, 1,491, 1,203, 1,195, and 1,207 g/d; SEM=81.1), OM digestibility ranked (P &lt; 0.05) CON, ION, CCO, and SBO &gt; MSL &gt; HSL (69.2, 57.6, 50.3, 66.3, 66.0, and 68.7%; SEM=1.57), and digestible OM intake was similar among treatments (P = 0.517; 697, 607, 589, 598, 635, and 690 g/d for CON, MSL, HSL, ION, CCO, and SBO, respectively; SEM=50.4). There were no interactions involving time in ruminal methane emission, which was greatest among treatments for CON (P &lt; 0.05) in MJ/d (1.39, 0.93, 0.90, 0.92, 0.85, and 0.96; SEM=0.069) and relative to digestible energy intake (20.6, 15.7, 16.8, 16.1, 13.7, and 13.9% for CON, MSL, HSL, ION, CCO, and SBO, respectively; SEM=1.223). In conclusion, dietary inclusion of Sericea lespedeza may offer a natural and sustainable means of decreasing ruminal methane emission by hair sheep as previously shown in goats, with a magnitude of impact similar to that of some other supplemental dietary ingredients.

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