In vitro Studies on the Toxicity of Culture Filtrates of Different Fungi on the Growth of Fusarium oxysporum f.sp. vasinfectum

AbstractRadopholus similis is one of the key pests of banana worldwide. In this study, nine endophytic Fusarium oxysporum isolates were screened for the production of secondary metabolites antagonistic to R. similis in culture. Undiluted and diluted culture filtrates were tested against motile stages and eggs of R. similis. All isolates tested demonstrated in vitro antagonistic activity, causing paralysis of R. similis motile stages. The percentage of paralysed nematodes increased with increase in the length of exposure time to culture filtrates. After 24 h exposure in culture filtrates up to 100% of the treated nematodes were paralysed compared to 26.5% in the control treatments. Nematode mortality rates after 24 h exposure in culture filtrates ranged from 76.4% to 100%. Paralysis was reversible at lower filtrate concentrations. Radopholus similis males were more sensitive to culture filtrates than females. Culture filtrates of all isolates demonstrated inhibitory effects on hatching of R. similis eggs. The results demonstrate the potential for using endophytic F. oxysporum as biological control agents against R. similis and for toxic derivatives from their secondary metabolism to be used as potential nematicides.

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In vitro Studies on Different Isolates of Fusarium oxysporum f sp. cubense Causing Panama Wilt of Banana in Lower Gangetic Plain

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2017.612.121 ◽

2017 ◽

Vol 6 (12) ◽

pp. 1072-1081

Author(s):

Debashis Rana ◽

Sahar Murmu ◽

Dilip Kumar Misra

Keyword(s):

Fusarium Oxysporum ◽

In Vitro Studies ◽

Gangetic Plain

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Bioactivity of Fusarium oxysporum f. sp. glycines and Sclerotium rolfsii filtrates on egg hatching, survival and infectivity of juveniles of Meloidogyne incognita race 2

Australian Journal of Experimental Agriculture ◽

10.1071/ea02129 ◽

2005 ◽

Vol 45 (1) ◽

pp. 99 ◽

Cited By ~ 3

Author(s):

O. K. Adekunle ◽

O. A. Akinsanmi

Keyword(s):

Fusarium Oxysporum ◽

Meloidogyne Incognita ◽

Sclerotium Rolfsii ◽

Egg Hatching ◽

Population Number ◽

Juvenile Mortality ◽

Soybean Seedlings ◽

Culture Filtrates ◽

Soybean Plants

The effects of culture filtrates of Fusarium oxysporum and Sclerotium rolfsii on egg hatching and juvenile survival of Meloidogyne incognita in vitro and impact of these filtrates on infectivity of M. incognita were investigated on soybean seedlings. Five- and 10-day-old filtrates of F. oxysporum caused 65 and 54% egg-hatching inhibition, while that of S. rolfsii caused 61 and 49% inhibition, respectively. Juveniles of M. incognita died within 6 days when incubated in 5-day-old filtrate of F. oxysporum, while the similar filtrate of S. rolfsii caused 100% juvenile mortality on the fifth day. Filtrates reduced root galling, egg population, number of adult females in soybean plants at harvest and also soil population. Culture filtrates could be used as source of biological nematicides.

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In Vitro Studies on Antifungal Activity of Aqueous Extract of Amorphophallus campanulatus against Fusarium oxysporum f. sp. cubense

Indo Global Journal of Pharmaceutical Sciences ◽

10.35652/igjps.2017.79 ◽

2017 ◽

Vol 07 (01) ◽

Author(s):

Sushmita Goswami ◽

Neha Bhadauria ◽

Shilpi, P.K Paul

Keyword(s):

Antifungal Activity ◽

Fusarium Oxysporum ◽

Aqueous Extract ◽

In Vitro Studies

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Study on usability of Fusarium oxysporum Schlecht.f.sp. tulipae Apt. metabolites for screening for basal rot resistance in tulip

Acta Agrobotanica ◽

10.5586/aa.2001.007 ◽

2013 ◽

Vol 54 (1) ◽

pp. 71-82 ◽

Cited By ~ 3

Author(s):

Małgorzata Podwyszyńska ◽

Czesław Skrzypczak ◽

Krzysztof Fatel ◽

Lech Michalczuk

Keyword(s):

Fusarium Oxysporum ◽

Shoot Growth ◽

Fusaric Acid ◽

Resistance Breeding ◽

Fungus Culture ◽

Basal Rot ◽

Culture Filtrates ◽

Selection Of ◽

Greenhouse Tests

The usefulness of fungus culture filtrates and fusaric acid as selecting agents for Fusarium resistance breeding in tulip was examined on in vitro cultures of shoots and embryonic calli of seven tulip genotypes differing in resistance to Fusarium oxysporum Schlecht. f. sp. tulipae Apt. (F.o.t.) and four virulent F.o.t. isolates. Fusaric acid influenced the shoot growth of all cultivars tested in a similar way, irrespectively of their greenhouse resistance to basal rot. Also, the sensitivity of calli of the cultivars studied to fusaric acid did not correspond with their resistance to F.o.t. evaluated in the greenhouse screening. The phytotoxity of F.o.t. culture filtrates did not depend on their fusaric acid contents. There was a negative correlation between cultivar's resistance to F.o.t in greenhouse tests and the sensitivity of their shoots to fungus culture filtrates in in vitro tests. This indicates that defence mechanism against F.o.t. in tulip tissue may have a nature of hypersensitive response. Considering the results of our study, it may be concluded that the use of fusaric acid or fungus culture filtrates for the in vitro selection of somaclones resistant to F.o.t. in tulip is not feasible.

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