Antifungal Activities (In vitro) of Some Plant Extracts and Smoke on Four Fungal Pathogens of Different Hosts

2002 ◽  
Vol 5 (3) ◽  
pp. 307-309 ◽  
Author(s):  
Shahidul Alam ◽  
Nargis Akhter ◽  
Most. Ferdousi Begum ◽  
M. Sabina Banu . ◽  
M. Rafiqul Islam . ◽  
...  
Author(s):  
Dewa Ngurah Suprapta

Abstract Plant fungal pathogens are frequently found as one of limiting factors for crop production. More than 10,000 species of fungi can cause disease in plants. To control the diseases, many farmers are still rely on the use of chemical fungicides, however most synthetic fungicides can cause acute toxicity, and some cause chronic toxicity as well. Thus, an appropriate technological improvement towards a more effective use of natural resources is required in agriculture to develop environmentally friendly sustainable farming system. This paper highlights the potential of extracts of tropical plants as antifungal agent to control plant fungal diseases. Information and data presented in this paper are mainly derived from selected and related references that previously published in the scientific journals. Many higher plants of tropical origin with fungicidal activities and their potential for fungal disease control of agricultural crops have been studied, however most of the studies have been done under in vitro condition. Some plant extracts showed strong antifungal activities on in vitro as well as in vivo tests, but some plant extracts showed significant antifungal activities on in vitro test, but did not obvious on in vivo tests. A great variation in antifungal activities were shown by plants extracts of different species and plant parts, in one hand, and on the other hand, variation was also observed on the responses of different fungal species to the same plant extract. Since the purpose of the use of plant extract is to control plant fungal diseases, the field trial is needed to ensure the stability of efficacy of certain plant extract. In addition, isolation and identification of active substances in the extracts is needed to assess possible mode of action and side effect of their use.


Author(s):  
Wuraola Funmi Ogundipe ◽  
Ayotunde Ajoke Pitan ◽  
Oluwafemi Michael Adedire ◽  
Adekunle Opeyemi Farinu ◽  
Barry Oyeyemi Oyewole

2010 ◽  
Vol 59 (2) ◽  
pp. 200-205 ◽  
Author(s):  
Ildikó Nyilasi ◽  
Sándor Kocsubé ◽  
Miklós Pesti ◽  
Gyöngyi Lukács ◽  
Tamás Papp ◽  
...  

The in vitro antifungal activities of primycin (PN) and various statins against some opportunistic pathogenic fungi were investigated. PN completely inhibited the growth of Candida albicans (MIC 64 μg ml−1) and Candida glabrata (MIC 32 μg ml−1), and was very effective against Paecilomyces variotii (MIC 2 μg ml−1), but had little effect on Aspergillus fumigatus, Aspergillus flavus or Rhizopus oryzae (MICs >64 μg ml−1). The fungi exhibited different degrees of sensitivity to the statins; fluvastatin (FLV) and simvastatin (SIM) exerted potent antifungal activities against a wide variety of clinically important fungal pathogens. Atorvastatin, rosuvastatin and lovastatin (LOV) had a slight effect against all fungal isolates tested, whereas pravastatin was completely ineffective. The in vitro interactions between PN and the different statins were investigated using a standard chequerboard titration method. When PN was combined with FLV, LOV or SIM, both synergistic and additive effects were observed. The extent of inhibition was higher when these compounds were applied together, and the concentrations of PN and the given statin needed to block fungal growth completely could be decreased by several dilution steps. Similar interactions were observed when the variability of the within-species sensitivities was investigated.


2011 ◽  
Vol 10 (14) ◽  
pp. 2625-2629 ◽  
Author(s):  
Yanar Yusuf ◽  
Kadioğlu Izzet ◽  
Gouml kccedil e Ayhan ◽  
Demirtaş İbrahim ◽  
Gouml ren Nezhun ◽  
...  

2021 ◽  
Vol 58 (04) ◽  
pp. 1263-1275
Author(s):  
Rashid Iqbal Khan

Plant extracts (PE’s) has emerged as a safer alternative to manage the fungal pathogens affecting tomato productivity. The current study aimed to evaluate the antimicrobial potential of methanolic fenugreek extract against Alternaria solani, a causal agent of early blight disease in tomato. Fenugreek extract was used at different concentrations of 5%, 10%, 15%, 20% and 25% under in vitro conditions. Results concluded that 25% fenugreek extract significantly reduced the radial growth (2.5 cm) of A. solani under in vitro conditions. Based on in vitro results, three concentrations (5%, 15% and 25%) of fenugreek extract was examined under greenhouse and field conditions. The outcomes expressed that 5% fenugreek extract reduced the disease severity up to 30.19% under greenhouse conditions and up to 40.53% under field trials. Although, application of fenugreek extract had exhibited non-significant impact on vegetative and reproductive growth parameters. However, its application had proved better results as compared to those plants which are infected with A. solani but received zero application of fenugreek extract. Furthermore, the effectiveness of plant extracts was evaluated by variant photosynthetic, antioxidative, polyphenolic and hypersensitive response of A. solani affected tomato plants. The 25% fenugreek extract application had augmented the chlorophyll pigments along with the significant increment of superoxide dismutase (174.16 U mg-1 protein), peroxidase (7.61 µmol min-1 g-1 protein) and catalase activity (4.73 nmol min-1 g-1 protein). Similar outcomes were observed regarding phenolic compounds, where 5% fenugreek extract application had enhanced flavonoid levels (26.62 mg QuE g-1), tannins (1.28 mg TE g-1 extract) and total phenolic contents (2.39 mg GAE g-1) in tomato leaves demonstrating its protective effect against early blight. In dose response, 25% fenugreek extract was most effective in reducing lipid peroxidation and enhancing H2O2 levels. The outcomes of study exhibited the fenugreek extract as an effective strategy to be used against A. solani to control early blight infection in tomato plants. Thus, it can serve as suitable fungicide alternative for resource-poor agriculture areas mainly in developing countries.


2012 ◽  
Vol 151 (4) ◽  
pp. 482-491 ◽  
Author(s):  
J. I. G. MASANGWA ◽  
T. A. S. AVELING ◽  
Q. KRITZINGER

SUMMARYThe aim of the present investigation was to evaluate the antifungal activities of plant extracts which can be used to control bean and cowpea anthracnose. Acetone, ethyl acetate and water extracts of Ipomoea batatas, Carica papaya, Allium sativum, Syzygium cordatum, Chlorophytum comosum and Agapanthus caulescens were screened in vitro for their antifungal activities against Colletotrichum lindemuthianum and Colletotrichum dematium of common bean and cowpea using the agar disc infusion and microtitre double-dilution techniques. The same extracts were then tested for antifungal activity in vivo as seed treatments against anthracnose disease. The water extracts of Carica and Syzygium were active against C. lindemuthianum and had minimum inhibitory concentrations (MICs) of 1·56 mg/ml. Syzygium, Allium and Chlorophytum water extracts were active against C. dematium and MICs were 3·13, 6·25 and 12·5 mg/ml, respectively. The MICs of Allium, Syzygium and Agapanthus acetone extracts were 0·78, 3·13 and 6·25 mg/ml, respectively, against C. lindemuthianum and 0·78, 6·25 and 3·13 mg/ml against C. dematium. Agapanthus water extracts and all the acetone extracts tested in vivo effectively reduced the incidence and severity of bean anthracnose disease in the greenhouse. Agapanthus acetone, Allium water, and both acetone and water extracts of Carica and Syzygium performed well in vivo in reducing cowpea anthracnose disease and compared well with reductions due to the application of the synthetic fungicide fludioxonil+mefenoxam (the commercial product Celest® XL) applied at 25 gai/l and also with levels in the non-inoculated control. The Agapanthus, Carica, Syzygium and Allium extracts were active on both Colletotrichum spp. in vitro and also reduced anthracnose disease of bean and cowpea and are potential seed treatments in anthracnose disease control. The easy seed treatment process and the accessibility of plants used in the present study could lead to high adoption of the use of the plant extracts as seed treatments by resource-poor, smallholder farmers.


2019 ◽  
Vol 6 (12) ◽  
pp. 123-132
Author(s):  
Jahangir Abdullah Koka ◽  
Abdul Hamid Wani ◽  
Mohd Yaqub Bhat ◽  
Shazia Parveen ◽  
Mohammad Afaan Fazili ◽  
...  

Egg plant Solanum melongena L. (Solanales: Solanaceae) is an important vegetable grown in Kashmir. It is attacked by number of fungal pathogens in storage and in the field. These fungi caused several fungal rot diseases resulting heavy losses to the growers. Therefore, present study was carried out to study the incidence and management of fungal rot of egg plant using some selected plant extracts. It was revealed from the study that Trichothecium roseum (Pers.) Link (1809) (Sordariomycetes: Hypocreales) causing decaying of egg plant under storage. Study was also undertaken to evaluate the efficacy of some plant extract against Trichothecium roseum on inhibition of spore germination and mycelial growth under in vitro conditions. It was observed from the results that amongst the plant extracts, plant extract of Ajuga bracteosa at highest concentration was found most effective against Trichothecium roseum and cause highest inhibition in the mycelial growth and spore germination followed by plant extract of Taraxicum officinale, Mentha arvensis and Iris kashmiriana at the same concentrations. Other concentrations of plant extracts also bought about significant reduction in mycelial growth and spore germination of the test fungus but to a lesser extent as compared to control.


Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
WR Cunha ◽  
FM Dos Santos ◽  
JA Peixoto ◽  
RCS Veneziani ◽  
AEM Crotti ◽  
...  

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